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The map label for this gene is ispH

Identifier: 88608647

GI number: 88608647

Start: 356761

End: 357672

Strand: Reverse

Name: ispH

Synonym: NSE_0438

Alternate gene names: 88608647

Gene position: 357672-356761 (Counterclockwise)

Preceding gene: 88608525

Following gene: 88608199

Centisome position: 41.64

GC content: 37.83

Gene sequence:

>912_bases
ATGCAAATATTCGTTGCCTGCCCTAGGGGCTTCTGCGCAGGGGTGGAACGTGCAATAGAAACAGTTGAGATAGCAATAAG
AAGATATCCAGAAAAAAAAATTTTTGTCTACCATGAGATAGTCCATAATTCCCGCGTTATACAGGATTTCAAAGCACGAG
GTGTGGATTTTATAGAGGACATCACTAAAGTAGAACCAAAGTCAGTATTGATTTTTAGCGCACACGGTGTTTCTGAAGAA
ATTGAAAAATACGTAAAGTCACTAGATTTAGTGCTTGTGGATGCAACCTGTCCACTGGTCTCAAAAATACATCGTGAAGT
TCAAAGATTTGAAAAAGAAGGTTTTATTATTCTAGTCATAGGAAATAGAAATCATGCTGAAATAATCGGAACAGTTGGAA
GAACTAAAACACAAGCCTATATCGTCCAAGATCCTGAAGATATAGATAATCTCCCCTCAATAACTGGAAAGTTGGCATAC
GTCACACAGAGTACACTTGCGCTTGAGTACACATCAACAATGATAAACAAGATAAAAGAAAAGTTCCCAAATGTGGTTGG
ACGGTCTGATGTATGTTATGCGACTCAAAACAGACAAGAAGCTGTTAAAGCACTTGCCAAAGAAGTGGATCTGATGTTGA
TAGTTGGTTCTACCAACAGTTCTAATTCAAGGAATCTAATGCAGGTCTCATCAAAGATATGCCAAAAAGCATTTCTCATA
GATAATTACAACGAAGTCGAACCCGATTGGTTTGAAGGAGTAGACAGAGTAGGAATCAGTTCGGGTGCTTCTGCTCCGGA
AATACTTGTACAAGATCTTGTATCATTTTTGAAAAAAAGATTTCCTTCTGCAGAAGTTTCAGACTTCAAATTTAAGGAAG
AGAAAGTGCAATTCAAGCTCCCAATTATATAG

Upstream 100 bases:

>100_bases
TGATCCTAACACAAAAATGTGCAAAACCCTGTGAGAATTGACAAATTCCTTGCTGAGTGCTACCGTGTACACAGTCTATT
CCTGTAAAGAAGAATTTAAG

Downstream 100 bases:

>100_bases
AGAAGTAAGATAGAACCCCAATTCCTATCTGATTTATAGTGCTTCCACCTTCGCCATCATATACAATTTGATGCTTCTCA
CACATATCTGCTATCAATAA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MQIFVACPRGFCAGVERAIETVEIAIRRYPEKKIFVYHEIVHNSRVIQDFKARGVDFIEDITKVEPKSVLIFSAHGVSEE
IEKYVKSLDLVLVDATCPLVSKIHREVQRFEKEGFIILVIGNRNHAEIIGTVGRTKTQAYIVQDPEDIDNLPSITGKLAY
VTQSTLALEYTSTMINKIKEKFPNVVGRSDVCYATQNRQEAVKALAKEVDLMLIVGSTNSSNSRNLMQVSSKICQKAFLI
DNYNEVEPDWFEGVDRVGISSGASAPEILVQDLVSFLKKRFPSAEVSDFKFKEEKVQFKLPII

Sequences:

>Translated_303_residues
MQIFVACPRGFCAGVERAIETVEIAIRRYPEKKIFVYHEIVHNSRVIQDFKARGVDFIEDITKVEPKSVLIFSAHGVSEE
IEKYVKSLDLVLVDATCPLVSKIHREVQRFEKEGFIILVIGNRNHAEIIGTVGRTKTQAYIVQDPEDIDNLPSITGKLAY
VTQSTLALEYTSTMINKIKEKFPNVVGRSDVCYATQNRQEAVKALAKEVDLMLIVGSTNSSNSRNLMQVSSKICQKAFLI
DNYNEVEPDWFEGVDRVGISSGASAPEILVQDLVSFLKKRFPSAEVSDFKFKEEKVQFKLPII
>Mature_303_residues
MQIFVACPRGFCAGVERAIETVEIAIRRYPEKKIFVYHEIVHNSRVIQDFKARGVDFIEDITKVEPKSVLIFSAHGVSEE
IEKYVKSLDLVLVDATCPLVSKIHREVQRFEKEGFIILVIGNRNHAEIIGTVGRTKTQAYIVQDPEDIDNLPSITGKLAY
VTQSTLALEYTSTMINKIKEKFPNVVGRSDVCYATQNRQEAVKALAKEVDLMLIVGSTNSSNSRNLMQVSSKICQKAFLI
DNYNEVEPDWFEGVDRVGISSGASAPEILVQDLVSFLKKRFPSAEVSDFKFKEEKVQFKLPII

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=307, Percent_Identity=43.6482084690554, Blast_Score=265, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_NEOSM (Q2GDX3)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506323.1
- STRING:   Q2GDX3
- GeneID:   3932177
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0438
- TIGR:   NSE_0438
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- PhylomeDB:   Q2GDX3
- ProtClustDB:   CLSK2528057
- BioCyc:   NSEN222891:NSE_0438-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34151; Mature: 34151

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIFVACPRGFCAGVERAIETVEIAIRRYPEKKIFVYHEIVHNSRVIQDFKARGVDFIED
CEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCHHHHHHHHHCCCHHHHH
ITKVEPKSVLIFSAHGVSEEIEKYVKSLDLVLVDATCPLVSKIHREVQRFEKEGFIILVI
HHHCCCCEEEEEECCCCHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHCCEEEEEE
GNRNHAEIIGTVGRTKTQAYIVQDPEDIDNLPSITGKLAYVTQSTLALEYTSTMINKIKE
CCCCCCEEEECCCCCCEEEEEEECCHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
KFPNVVGRSDVCYATQNRQEAVKALAKEVDLMLIVGSTNSSNSRNLMQVSSKICQKAFLI
HCCHHCCCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHC
DNYNEVEPDWFEGVDRVGISSGASAPEILVQDLVSFLKKRFPSAEVSDFKFKEEKVQFKL
CCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEC
PII
CCC
>Mature Secondary Structure
MQIFVACPRGFCAGVERAIETVEIAIRRYPEKKIFVYHEIVHNSRVIQDFKARGVDFIED
CEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCHHHHHHHHHCCCHHHHH
ITKVEPKSVLIFSAHGVSEEIEKYVKSLDLVLVDATCPLVSKIHREVQRFEKEGFIILVI
HHHCCCCEEEEEECCCCHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHCCEEEEEE
GNRNHAEIIGTVGRTKTQAYIVQDPEDIDNLPSITGKLAYVTQSTLALEYTSTMINKIKE
CCCCCCEEEECCCCCCEEEEEEECCHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
KFPNVVGRSDVCYATQNRQEAVKALAKEVDLMLIVGSTNSSNSRNLMQVSSKICQKAFLI
HCCHHCCCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHC
DNYNEVEPDWFEGVDRVGISSGASAPEILVQDLVSFLKKRFPSAEVSDFKFKEEKVQFKL
CCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEC
PII
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA