Click here to switch to the map view.

The map label for this gene is trmB

Identifier: 88608619

GI number: 88608619

Start: 817520

End: 818173

Strand: Reverse

Name: trmB

Synonym: NSE_0922

Alternate gene names: 88608619

Gene position: 818173-817520 (Counterclockwise)

Preceding gene: 88608266

Following gene: 88608489

Centisome position: 95.25

GC content: 41.44

Gene sequence:

>654_bases
GTGTTTGTACACAATGAAGTAAATCTGCGGCTGGAAAAGAAACAGTTTCTCAGATCTTATGGGAGGCGTCGTGGTCGTAA
TTTTAGGGAGAATAAGCTTGTCGGTTTAGAAAGGTTTCTTGCGTCTCATGAGAAACTCGAAAATCTCAAGAGTGAGATCG
TATTGGAGGTTGGTTTTGGTTTCGGTGAACACATATTAAAGAAGGCTATAGATTATCCGGACAAGGTATTCGTCGGCGCT
GAAGTATACGTAAATGGTATAGCCAGGCTCCTAGAGCAAGTGGAGCTTCATGAACTATCAAATGTTCTAATTTGGAATGA
CGATGTTCGTCTTCTGTTGGAAAAGTTGCTTTGTAAAGTTTTTCATAAAGTATACATACTATTTCCTGATCCCTGGCCCA
AGAGGAGGCACCACAAACGTAGGATTGTAGATGTGCCATTTTTTCAGCTCCTGAGTCGTAGCTTGCATCCATTAGCTGAG
GTTGTCGTTGCAACTGACGATCACGATTATGCTCAGCAAATATATCAATCTGCCTTGGAAGTCTTTTCTAAAGTTGAGAA
TACGGAAATACCAAAGAATGCCAGCCGTTTCGCATCAAAAGCAAGTAACAGGATATTCGGTTACAAGATGGTATATGACG
CAACTGACGCATGA

Upstream 100 bases:

>100_bases
TCGTAGGAGACCTGAGACTGAATAATAATGAATTGTGAGGGTATTATACTTTTTCTTTTCAGGTGTTAGCCTTTCGTTTT
TAGGTGATCTTTGAGTCGGT

Downstream 100 bases:

>100_bases
ATTTTTTCGTTTAGTTCTGTTCTCTTATTTGTGAATAGAACGCGTACCAGGTGTGTTTCGGAACTACCAGAGCATTCTCC
AAGACTTTTTGCTACATTGT

Product: putative tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MFVHNEVNLRLEKKQFLRSYGRRRGRNFRENKLVGLERFLASHEKLENLKSEIVLEVGFGFGEHILKKAIDYPDKVFVGA
EVYVNGIARLLEQVELHELSNVLIWNDDVRLLLEKLLCKVFHKVYILFPDPWPKRRHHKRRIVDVPFFQLLSRSLHPLAE
VVVATDDHDYAQQIYQSALEVFSKVENTEIPKNASRFASKASNRIFGYKMVYDATDA

Sequences:

>Translated_217_residues
MFVHNEVNLRLEKKQFLRSYGRRRGRNFRENKLVGLERFLASHEKLENLKSEIVLEVGFGFGEHILKKAIDYPDKVFVGA
EVYVNGIARLLEQVELHELSNVLIWNDDVRLLLEKLLCKVFHKVYILFPDPWPKRRHHKRRIVDVPFFQLLSRSLHPLAE
VVVATDDHDYAQQIYQSALEVFSKVENTEIPKNASRFASKASNRIFGYKMVYDATDA
>Mature_217_residues
MFVHNEVNLRLEKKQFLRSYGRRRGRNFRENKLVGLERFLASHEKLENLKSEIVLEVGFGFGEHILKKAIDYPDKVFVGA
EVYVNGIARLLEQVELHELSNVLIWNDDVRLLLEKLLCKVFHKVYILFPDPWPKRRHHKRRIVDVPFFQLLSRSLHPLAE
VVVATDDHDYAQQIYQSALEVFSKVENTEIPKNASRFASKASNRIFGYKMVYDATDA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=219, Percent_Identity=30.5936073059361, Blast_Score=93, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_NEOSM (Q2GCL0)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506786.1
- ProteinModelPortal:   Q2GCL0
- SMR:   Q2GCL0
- STRING:   Q2GCL0
- GeneID:   3932004
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0922
- TIGR:   NSE_0922
- eggNOG:   COG0220
- OMA:   GRNFREN
- PhylomeDB:   Q2GCL0
- ProtClustDB:   CLSK2528039
- BioCyc:   NSEN222891:NSE_0922-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 25438; Mature: 25438

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: BINDING 56-56 BINDING 81-81 BINDING 108-108 BINDING 130-130 BINDING 134-134 BINDING 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVHNEVNLRLEKKQFLRSYGRRRGRNFRENKLVGLERFLASHEKLENLKSEIVLEVGFG
CCCCCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEHHHCCC
FGEHILKKAIDYPDKVFVGAEVYVNGIARLLEQVELHELSNVLIWNDDVRLLLEKLLCKV
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHH
FHKVYILFPDPWPKRRHHKRRIVDVPFFQLLSRSLHPLAEVVVATDDHDYAQQIYQSALE
HHHEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VFSKVENTEIPKNASRFASKASNRIFGYKMVYDATDA
HHHHHHCCCCCCHHHHHHHHHCCCEEEEEEEECCCCC
>Mature Secondary Structure
MFVHNEVNLRLEKKQFLRSYGRRRGRNFRENKLVGLERFLASHEKLENLKSEIVLEVGFG
CCCCCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEHHHCCC
FGEHILKKAIDYPDKVFVGAEVYVNGIARLLEQVELHELSNVLIWNDDVRLLLEKLLCKV
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHH
FHKVYILFPDPWPKRRHHKRRIVDVPFFQLLSRSLHPLAEVVVATDDHDYAQQIYQSALE
HHHEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VFSKVENTEIPKNASRFASKASNRIFGYKMVYDATDA
HHHHHHCCCCCCHHHHHHHHHCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA