Click here to switch to the map view.

The map label for this gene is mraW

Identifier: 88608489

GI number: 88608489

Start: 816499

End: 817389

Strand: Reverse

Name: mraW

Synonym: NSE_0921

Alternate gene names: 88608489

Gene position: 817389-816499 (Counterclockwise)

Preceding gene: 88608619

Following gene: 88608837

Centisome position: 95.16

GC content: 40.74

Gene sequence:

>891_bases
ATGAGTGTTACTGGTGGCTCAAAAGATGAGATATTACACAAGCCAGTTCTTTTGAGCGAAGCTCTGAAATTTCTTGCTCC
GAAGAATGGTGGTGTTTATGTAGATGCAACATTTGGTGCAGGCGGTTACACCAGAGCTATTTTGAGCAGTGTCGATTGTT
TTGTGTACGCTATTGACCGTGATGAGACAGTAAGACGATTCTTCCAGGTTATAGAGAATGATTTTCCCAAGCGTACAAAC
TTCATAAATGGTAATTTTGTGGATATCGGATCTTTGCTGGGTGAAGTGAAGGTGGACGGGATTGTGTTTGACTTAGGTGT
TTCAACAATGCAATTGAAGGTTGCTGATAGAGGTTTCTCTTTTCTTCACGAGGGTCCTTTAGACATGCGTATGGATAGAA
GTACTCTACTAACGGCTGAGACAGTTGTTAATAGTTACACTGAAGTGAAAATTGCCTCCATTATCTACCAGTTTGGTGAA
GAGAGAATGTCAAGGAAAATAGCTCATGCAATTGTTAATGCAAGACGCAAAAAAAGAATCACGACAACCAGCAGTCTAGC
AGAAATCGTGCGATCTTGTTTTCCGAAAAGATACTATAAGATAGATCCGGCGACAAAGACTTTTCAGGCCCTGAGAATTT
TCATAAATGATGAATTGGGTGCCCTTGAATGCGGACTGAGAACAGCGCTGACAATGCTGAAAGTTGGAAGTAGGGCAGTT
GTTGTTAGCTTCCATTCTCTTGAAGATAGAATAGTCAAACATTTATTTAGAAGTGTTCATGGAAATGGGTTTAATTTGCT
CACGAAGAAGATTGTTGTTCCAAGTAGAGATGAAGTGAGAGAGAATCCAAGCTCCCGTTCCGCAAGAATGAGAGTGATAG
AGAAGGTATGA

Upstream 100 bases:

>100_bases
GTGAATAGAACGCGTACCAGGTGTGTTTCGGAACTACCAGAGCATTCTCCAAGACTTTTTGCTACATTGTTAACTGGATA
AAGTGTTCTTGAGTGCTCAA

Downstream 100 bases:

>100_bases
AAAGCTCACGTTGTGCTCATGAAGTACAATTCTTAGAGCTTCGTGCTCATTAAAATTGTTTTTGTACTAATTTTTGGATT
AAGTGTTGTAAATCGCGCAT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MSVTGGSKDEILHKPVLLSEALKFLAPKNGGVYVDATFGAGGYTRAILSSVDCFVYAIDRDETVRRFFQVIENDFPKRTN
FINGNFVDIGSLLGEVKVDGIVFDLGVSTMQLKVADRGFSFLHEGPLDMRMDRSTLLTAETVVNSYTEVKIASIIYQFGE
ERMSRKIAHAIVNARRKKRITTTSSLAEIVRSCFPKRYYKIDPATKTFQALRIFINDELGALECGLRTALTMLKVGSRAV
VVSFHSLEDRIVKHLFRSVHGNGFNLLTKKIVVPSRDEVRENPSSRSARMRVIEKV

Sequences:

>Translated_296_residues
MSVTGGSKDEILHKPVLLSEALKFLAPKNGGVYVDATFGAGGYTRAILSSVDCFVYAIDRDETVRRFFQVIENDFPKRTN
FINGNFVDIGSLLGEVKVDGIVFDLGVSTMQLKVADRGFSFLHEGPLDMRMDRSTLLTAETVVNSYTEVKIASIIYQFGE
ERMSRKIAHAIVNARRKKRITTTSSLAEIVRSCFPKRYYKIDPATKTFQALRIFINDELGALECGLRTALTMLKVGSRAV
VVSFHSLEDRIVKHLFRSVHGNGFNLLTKKIVVPSRDEVRENPSSRSARMRVIEKV
>Mature_295_residues
SVTGGSKDEILHKPVLLSEALKFLAPKNGGVYVDATFGAGGYTRAILSSVDCFVYAIDRDETVRRFFQVIENDFPKRTNF
INGNFVDIGSLLGEVKVDGIVFDLGVSTMQLKVADRGFSFLHEGPLDMRMDRSTLLTAETVVNSYTEVKIASIIYQFGEE
RMSRKIAHAIVNARRKKRITTTSSLAEIVRSCFPKRYYKIDPATKTFQALRIFINDELGALECGLRTALTMLKVGSRAVV
VSFHSLEDRIVKHLFRSVHGNGFNLLTKKIVVPSRDEVRENPSSRSARMRVIEKV

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=38.8724035608309, Blast_Score=215, Evalue=4e-56,
Organism=Homo sapiens, GI165377202, Length=188, Percent_Identity=42.0212765957447, Blast_Score=145, Evalue=6e-35,
Organism=Escherichia coli, GI1786270, Length=310, Percent_Identity=38.7096774193548, Blast_Score=206, Evalue=1e-54,
Organism=Drosophila melanogaster, GI62472493, Length=332, Percent_Identity=36.4457831325301, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI28571637, Length=332, Percent_Identity=36.4457831325301, Blast_Score=181, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_NEOSM (Q2GCL1)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506785.1
- ProteinModelPortal:   Q2GCL1
- SMR:   Q2GCL1
- STRING:   Q2GCL1
- GeneID:   3932131
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0921
- TIGR:   NSE_0921
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   Q2GCL1
- ProtClustDB:   PRK00050
- BioCyc:   NSEN222891:NSE_0921-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 33162; Mature: 33031

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: BINDING 59-59 BINDING 86-86 BINDING 104-104 BINDING 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTGGSKDEILHKPVLLSEALKFLAPKNGGVYVDATFGAGGYTRAILSSVDCFVYAIDR
CCCCCCCHHHHHHCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCEEEEEECC
DETVRRFFQVIENDFPKRTNFINGNFVDIGSLLGEVKVDGIVFDLGVSTMQLKVADRGFS
CHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHEECCEEEECCCCEEEEEECCCCCH
FLHEGPLDMRMDRSTLLTAETVVNSYTEVKIASIIYQFGEERMSRKIAHAIVNARRKKRI
HHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TTTSSLAEIVRSCFPKRYYKIDPATKTFQALRIFINDELGALECGLRTALTMLKVGSRAV
CHHHHHHHHHHHHCCHHEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEE
VVSFHSLEDRIVKHLFRSVHGNGFNLLTKKIVVPSRDEVRENPSSRSARMRVIEKV
EEEHHHHHHHHHHHHHHHHCCCCCEEHHHEEECCCHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SVTGGSKDEILHKPVLLSEALKFLAPKNGGVYVDATFGAGGYTRAILSSVDCFVYAIDR
CCCCCCHHHHHHCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCEEEEEECC
DETVRRFFQVIENDFPKRTNFINGNFVDIGSLLGEVKVDGIVFDLGVSTMQLKVADRGFS
CHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHEECCEEEECCCCEEEEEECCCCCH
FLHEGPLDMRMDRSTLLTAETVVNSYTEVKIASIIYQFGEERMSRKIAHAIVNARRKKRI
HHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TTTSSLAEIVRSCFPKRYYKIDPATKTFQALRIFINDELGALECGLRTALTMLKVGSRAV
CHHHHHHHHHHHHCCHHEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEE
VVSFHSLEDRIVKHLFRSVHGNGFNLLTKKIVVPSRDEVRENPSSRSARMRVIEKV
EEEHHHHHHHHHHHHHHHHCCCCCEEHHHEEECCCHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA