Click here to switch to the map view.

The map label for this gene is nadE

Identifier: 88608610

GI number: 88608610

Start: 79285

End: 80091

Strand: Reverse

Name: nadE

Synonym: NSE_0097

Alternate gene names: 88608610

Gene position: 80091-79285 (Counterclockwise)

Preceding gene: 88607972

Following gene: 88608400

Centisome position: 9.32

GC content: 41.51

Gene sequence:

>807_bases
TTGGAAAAATACTATTTATCTGTGCTCCATGAACTGAGAAAGTACTTAAAAAACACAGACATGCTAGATGTAATCGTTGG
ACTTTCTGGAGGAATAGACTCGGCCTTAGTTGCAGCAATCGCGACTGATGCATTTGGTAATAGTCGCGTGCGAACCGTGA
TGATGCCTTCTCCCTATACAAGCACCGATAGTCTCGATGATGCACTAGAGATCGCTCATAGGTTGGGCGTGGCTCACACA
GTGATCCGCATAGATGGACTGCTTCAGTCATTCAAAGAGGTTTTTTCACACTGCGTAACATGGGAAGAACAGGATATCAC
AGAACAGAACTTGCAATCACGTATACGCGCTGTCACCTTGATGGGACTTTCAAATAAAACCAAGAGCATGGTGCTTTCGA
CCAGCAACAGGTCTGAGATCCTTACAGGTTACTTTACCCTCTATGGCGATTCATGCGGCGGATATGCACCTATTTCACCA
TTTTTAAAAACCCAGGTTTACAAACTTGCACAATGGCGTAATGGCAATATACCAGAAAGCAGTATGCTACAGAAGATAAG
TATTATCCCAGAATCGGTATTAAGAAAAGCTCCAACCGCTGAGTTAAAGTACAACCAGAGAGATTGTGACGAACTTCCAG
AGTATGAAATACTCGACCAAATTTTAACCGCCATCTCATGTAAACAAGATTTAACAATTTTAAGTATAGATGAACGAATA
GTCTCAATGGTCGTTGAACGGGTTAAAAAAGCTCAATACAAGAGAAGATACTTACCTATTGGGCCTGAACTACTTGAGAT
TAATTAG

Upstream 100 bases:

>100_bases
TCTACCCAAGGTTTTCCGGTGAATGCAAACGTATGATCTCTTTACCTCGCACAAAGTAAAGGTTATCTGTAATGCTTACG
TATAAAAACATCACCCTAAA

Downstream 100 bases:

>100_bases
AGTCCTAGATTCTTGCTTAAATTGAGCATATACTTCAACTATTCTTTAATTTCTATTCTATAACATCAACTAAGCATTGA
TGTTGTATTATATGCTGCAA

Product: NAD+ synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MEKYYLSVLHELRKYLKNTDMLDVIVGLSGGIDSALVAAIATDAFGNSRVRTVMMPSPYTSTDSLDDALEIAHRLGVAHT
VIRIDGLLQSFKEVFSHCVTWEEQDITEQNLQSRIRAVTLMGLSNKTKSMVLSTSNRSEILTGYFTLYGDSCGGYAPISP
FLKTQVYKLAQWRNGNIPESSMLQKISIIPESVLRKAPTAELKYNQRDCDELPEYEILDQILTAISCKQDLTILSIDERI
VSMVVERVKKAQYKRRYLPIGPELLEIN

Sequences:

>Translated_268_residues
MEKYYLSVLHELRKYLKNTDMLDVIVGLSGGIDSALVAAIATDAFGNSRVRTVMMPSPYTSTDSLDDALEIAHRLGVAHT
VIRIDGLLQSFKEVFSHCVTWEEQDITEQNLQSRIRAVTLMGLSNKTKSMVLSTSNRSEILTGYFTLYGDSCGGYAPISP
FLKTQVYKLAQWRNGNIPESSMLQKISIIPESVLRKAPTAELKYNQRDCDELPEYEILDQILTAISCKQDLTILSIDERI
VSMVVERVKKAQYKRRYLPIGPELLEIN
>Mature_268_residues
MEKYYLSVLHELRKYLKNTDMLDVIVGLSGGIDSALVAAIATDAFGNSRVRTVMMPSPYTSTDSLDDALEIAHRLGVAHT
VIRIDGLLQSFKEVFSHCVTWEEQDITEQNLQSRIRAVTLMGLSNKTKSMVLSTSNRSEILTGYFTLYGDSCGGYAPISP
FLKTQVYKLAQWRNGNIPESSMLQKISIIPESVLRKAPTAELKYNQRDCDELPEYEILDQILTAISCKQDLTILSIDERI
VSMVVERVKKAQYKRRYLPIGPELLEIN

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 30217; Mature: 30217

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKYYLSVLHELRKYLKNTDMLDVIVGLSGGIDSALVAAIATDAFGNSRVRTVMMPSPYT
CCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCC
STDSLDDALEIAHRLGVAHTVIRIDGLLQSFKEVFSHCVTWEEQDITEQNLQSRIRAVTL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
MGLSNKTKSMVLSTSNRSEILTGYFTLYGDSCGGYAPISPFLKTQVYKLAQWRNGNIPES
HCCCCCCHHEEEECCCCCHHHEEHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHH
SMLQKISIIPESVLRKAPTAELKYNQRDCDELPEYEILDQILTAISCKQDLTILSIDERI
HHHHHHHHCHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCEEEEHHHHH
VSMVVERVKKAQYKRRYLPIGPELLEIN
HHHHHHHHHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure
MEKYYLSVLHELRKYLKNTDMLDVIVGLSGGIDSALVAAIATDAFGNSRVRTVMMPSPYT
CCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCC
STDSLDDALEIAHRLGVAHTVIRIDGLLQSFKEVFSHCVTWEEQDITEQNLQSRIRAVTL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
MGLSNKTKSMVLSTSNRSEILTGYFTLYGDSCGGYAPISPFLKTQVYKLAQWRNGNIPES
HCCCCCCHHEEEECCCCCHHHEEHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHH
SMLQKISIIPESVLRKAPTAELKYNQRDCDELPEYEILDQILTAISCKQDLTILSIDERI
HHHHHHHHCHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCEEEEHHHHH
VSMVVERVKKAQYKRRYLPIGPELLEIN
HHHHHHHHHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]