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The map label for this gene is fmt

Identifier: 88608400

GI number: 88608400

Start: 78013

End: 78936

Strand: Reverse

Name: fmt

Synonym: NSE_0094

Alternate gene names: 88608400

Gene position: 78936-78013 (Counterclockwise)

Preceding gene: 88608610

Following gene: 88608026

Centisome position: 9.19

GC content: 40.58

Gene sequence:

>924_bases
GTGAAAATACTTTTTATGGGATCATCCGCGTTTGCGGTTCCAACGCTTTTGAAACTCATAGCAGACCCGCAATTCGAGGT
CTTAGAGGTCTATACCAAACCTCCAAAACCTAAGGGCCGCGGCCTCTTAACTTCGATGACGCAAGTACATTCGACATCTT
TGGAGTATGGCCTTTCTGTAAAAACACCTTTTTCTCTAAAAGGAGAGGAACTTCCTCCTTGTGACGTAATTGTTGTTGTC
TCTTATGGTCTTATCATACCAGCTAAATTACTATCACATCCAAAGTTGGTACCTCTTAATATTCACCCATCTCTTTTACC
TAGATGGCGTGGTCCGTCGCCAATTCAATATACAATCTTGAAGGGAGATAAAGAGGCAGGCGTGTCTATAATACGCGTCA
CACCTGAACTAGATGCTGGAGCGATATACATACAAAAGGCTATTCCTCTTGATGGAACCGAGACATATTCAATTTTACAC
GATCGACTCGCGAATCTCGGTGCCTCAATGCTTCATGGCGTATTGGAAACTTTGAACACAATCAGCCCTGTACCACAAGC
TGAAGAACAAGCTACCTATTCAAAGAAACTTCCTAATTCAGTAAGCATTGATTTCTCTGAAGGGATAGCATCATTTTTAA
GAAAGCTAAGAGTTTTTGGTGCTGTTTCAATCGAGTTATTTGGCAAGCACATAAAGATAGTAGAAGTTTGCAAGACCGCG
GATTCACCATCTGCTGATGTGGGTGTCAAAAAAGACTCTAAAAAGATCCTTCTTCAATTTAAGGATGGATGTGCAACCAT
AAAAACGGTGAAACCAGAGAATAAAAGGGAAATGTCCACAGAAGATTTCCTCAACGGAATACAGAGTAAATATGGGACCC
ATGCTGACTCGCATGTTTTTATAGATTATAAAGAAAACTATTAA

Upstream 100 bases:

>100_bases
GCTATAGCTCAGTCGGTTAGAGCACTCCGCTTATAACGGAGAGGTCGCTGGTTCAAGTCCAGTTAGCCCTACTGGATGGT
TCTAATTTGCTTCGAGCTCA

Downstream 100 bases:

>100_bases
CTTAACCCACTACCTAGCAGCAGAACCCCTTGTCTTCACACCGGATGGAAACGGCTTTTTCACGACAACGCTCGCAACAC
GCAGCTATAAACCACCACAA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKILFMGSSAFAVPTLLKLIADPQFEVLEVYTKPPKPKGRGLLTSMTQVHSTSLEYGLSVKTPFSLKGEELPPCDVIVVV
SYGLIIPAKLLSHPKLVPLNIHPSLLPRWRGPSPIQYTILKGDKEAGVSIIRVTPELDAGAIYIQKAIPLDGTETYSILH
DRLANLGASMLHGVLETLNTISPVPQAEEQATYSKKLPNSVSIDFSEGIASFLRKLRVFGAVSIELFGKHIKIVEVCKTA
DSPSADVGVKKDSKKILLQFKDGCATIKTVKPENKREMSTEDFLNGIQSKYGTHADSHVFIDYKENY

Sequences:

>Translated_307_residues
MKILFMGSSAFAVPTLLKLIADPQFEVLEVYTKPPKPKGRGLLTSMTQVHSTSLEYGLSVKTPFSLKGEELPPCDVIVVV
SYGLIIPAKLLSHPKLVPLNIHPSLLPRWRGPSPIQYTILKGDKEAGVSIIRVTPELDAGAIYIQKAIPLDGTETYSILH
DRLANLGASMLHGVLETLNTISPVPQAEEQATYSKKLPNSVSIDFSEGIASFLRKLRVFGAVSIELFGKHIKIVEVCKTA
DSPSADVGVKKDSKKILLQFKDGCATIKTVKPENKREMSTEDFLNGIQSKYGTHADSHVFIDYKENY
>Mature_307_residues
MKILFMGSSAFAVPTLLKLIADPQFEVLEVYTKPPKPKGRGLLTSMTQVHSTSLEYGLSVKTPFSLKGEELPPCDVIVVV
SYGLIIPAKLLSHPKLVPLNIHPSLLPRWRGPSPIQYTILKGDKEAGVSIIRVTPELDAGAIYIQKAIPLDGTETYSILH
DRLANLGASMLHGVLETLNTISPVPQAEEQATYSKKLPNSVSIDFSEGIASFLRKLRVFGAVSIELFGKHIKIVEVCKTA
DSPSADVGVKKDSKKILLQFKDGCATIKTVKPENKREMSTEDFLNGIQSKYGTHADSHVFIDYKENY

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI164663775, Length=314, Percent_Identity=31.5286624203822, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI21614513, Length=323, Percent_Identity=26.9349845201238, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI238814322, Length=139, Percent_Identity=33.0935251798561, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789683, Length=299, Percent_Identity=33.7792642140468, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1788589, Length=191, Percent_Identity=25.130890052356, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI133930964, Length=227, Percent_Identity=29.0748898678414, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319458, Length=145, Percent_Identity=35.8620689655172, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45550868, Length=310, Percent_Identity=28.0645161290323, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI28571984, Length=236, Percent_Identity=30.5084745762712, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585660, Length=257, Percent_Identity=26.8482490272374, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 33687; Mature: 33687

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILFMGSSAFAVPTLLKLIADPQFEVLEVYTKPPKPKGRGLLTSMTQVHSTSLEYGLSV
CEEEEECCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCEEE
KTPFSLKGEELPPCDVIVVVSYGLIIPAKLLSHPKLVPLNIHPSLLPRWRGPSPIQYTIL
CCCCCCCCCCCCCCCEEEEEHHCHHHHHHHHCCCCEEEEECCHHHCCCCCCCCCEEEEEE
KGDKEAGVSIIRVTPELDAGAIYIQKAIPLDGTETYSILHDRLANLGASMLHGVLETLNT
ECCHHHCEEEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISPVPQAEEQATYSKKLPNSVSIDFSEGIASFLRKLRVFGAVSIELFGKHIKIVEVCKTA
CCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCEEEEHHCCHHHHHHHHHCC
DSPSADVGVKKDSKKILLQFKDGCATIKTVKPENKREMSTEDFLNGIQSKYGTHADSHVF
CCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCHHCCCHHHHHHHHHHHHCCCCCCEEE
IDYKENY
EEECCCC
>Mature Secondary Structure
MKILFMGSSAFAVPTLLKLIADPQFEVLEVYTKPPKPKGRGLLTSMTQVHSTSLEYGLSV
CEEEEECCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCEEE
KTPFSLKGEELPPCDVIVVVSYGLIIPAKLLSHPKLVPLNIHPSLLPRWRGPSPIQYTIL
CCCCCCCCCCCCCCCEEEEEHHCHHHHHHHHCCCCEEEEECCHHHCCCCCCCCCEEEEEE
KGDKEAGVSIIRVTPELDAGAIYIQKAIPLDGTETYSILHDRLANLGASMLHGVLETLNT
ECCHHHCEEEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISPVPQAEEQATYSKKLPNSVSIDFSEGIASFLRKLRVFGAVSIELFGKHIKIVEVCKTA
CCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCEEEEHHCCHHHHHHHHHCC
DSPSADVGVKKDSKKILLQFKDGCATIKTVKPENKREMSTEDFLNGIQSKYGTHADSHVF
CCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCHHCCCHHHHHHHHHHHHCCCCCCEEE
IDYKENY
EEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA