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The map label for this gene is gpsA

Identifier: 88608564

GI number: 88608564

Start: 193716

End: 194720

Strand: Reverse

Name: gpsA

Synonym: NSE_0228

Alternate gene names: 88608564

Gene position: 194720-193716 (Counterclockwise)

Preceding gene: 88608186

Following gene: 88608181

Centisome position: 22.67

GC content: 39.6

Gene sequence:

>1005_bases
ATGGTGCTCATGGACTCAGTCGTTATTGGTGGGGGAGCATGGGGAACTGCAATCGCAAACCTTCTCGCGTTCAACACCCA
ACGCGTGACAATTTTTTGCAGGAATACAACGGTTATCGATAGTATAAACAAGAGACATATTAACACTAAATACCTACCTA
CTTTCCCGCTCAACAAAAACATTTCCGCTACGAGCAGGATGGACGTCTTAAAAAATGCGGAGTTGATCTTCGTAGCCGTT
CCATCTCAGAGCATGCGAGAACTCCTCCAAAAAGTCAAAGAAAATATCAAAGAGAGTGTCCAAATCATACTGTGTAATAA
GGGAATAGAGAGAGAATCTTTGCTTTTGATGAGTGAAGTCGTTCATGAAGAACTTCCTAAAAATGATATCTTCGTCCTTT
CTGGACCAAATTTTGCACATGAAGTACTTAGCAAGAAACCTTCTTTTAGTAACTTAGCTGGGCGTAACAAAACCAGTTAT
GACAAGATAGCGAATGCGCTCTCGACGGAAACATTTTTTACAAAATATATCACTGATATTAACGGCACACAGATACTCGG
GGCTTTCAAGAACGTTATAGCAATAATTTGTGGCCTACTGGTTCGTATGGATGCAGGTTCGAATACTCTGTCAGCGCTTA
TGAGCTTAGCATTGGAGGAAGCTCGCTCTTTCATAACAATAAAGAATGGAAATCCAGATACTATAATGGAATTTTGTGGG
ATAGGTGATCTCGTTCTCACATGCTTTTCAAATAAATCACGCAATTTTAGATATGGTTACAGACTTGTCGATGGCTACAG
TGAAAACGCGTTAGTTGAAGGAAAATCAACCTTGGAATCATTACACGAATTGGCTCGTATACATAACATAAACTGTGTAC
TAACAAATACGTTATACACTGTAACTCAATTTAATTCATATGGCACGAGCTCTTTTGAGCAAGATATCAAACGGGAGCTC
AACTCAGCTTTTATGAGCTTGTTAGGTTGCGCGAAAAATCCATAA

Upstream 100 bases:

>100_bases
CTTCATCGTAACACCACATGATCATTTCAGCTTTGTGGTTAACGGGCTTCTGTGATGACAGATAGACAGCTTTGATGTAC
CATTCTATCGACAATCAAGT

Downstream 100 bases:

>100_bases
AATTGGATAAGTAATCCATTAAGAATAGGGATCGCTATGATACTACTACAATCCTGAGAAATGGCGCTTCTGACATATCC
TCAAGTATGTTAATATAAAG

Product: glycerol-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MVLMDSVVIGGGAWGTAIANLLAFNTQRVTIFCRNTTVIDSINKRHINTKYLPTFPLNKNISATSRMDVLKNAELIFVAV
PSQSMRELLQKVKENIKESVQIILCNKGIERESLLLMSEVVHEELPKNDIFVLSGPNFAHEVLSKKPSFSNLAGRNKTSY
DKIANALSTETFFTKYITDINGTQILGAFKNVIAIICGLLVRMDAGSNTLSALMSLALEEARSFITIKNGNPDTIMEFCG
IGDLVLTCFSNKSRNFRYGYRLVDGYSENALVEGKSTLESLHELARIHNINCVLTNTLYTVTQFNSYGTSSFEQDIKREL
NSAFMSLLGCAKNP

Sequences:

>Translated_334_residues
MVLMDSVVIGGGAWGTAIANLLAFNTQRVTIFCRNTTVIDSINKRHINTKYLPTFPLNKNISATSRMDVLKNAELIFVAV
PSQSMRELLQKVKENIKESVQIILCNKGIERESLLLMSEVVHEELPKNDIFVLSGPNFAHEVLSKKPSFSNLAGRNKTSY
DKIANALSTETFFTKYITDINGTQILGAFKNVIAIICGLLVRMDAGSNTLSALMSLALEEARSFITIKNGNPDTIMEFCG
IGDLVLTCFSNKSRNFRYGYRLVDGYSENALVEGKSTLESLHELARIHNINCVLTNTLYTVTQFNSYGTSSFEQDIKREL
NSAFMSLLGCAKNP
>Mature_334_residues
MVLMDSVVIGGGAWGTAIANLLAFNTQRVTIFCRNTTVIDSINKRHINTKYLPTFPLNKNISATSRMDVLKNAELIFVAV
PSQSMRELLQKVKENIKESVQIILCNKGIERESLLLMSEVVHEELPKNDIFVLSGPNFAHEVLSKKPSFSNLAGRNKTSY
DKIANALSTETFFTKYITDINGTQILGAFKNVIAIICGLLVRMDAGSNTLSALMSLALEEARSFITIKNGNPDTIMEFCG
IGDLVLTCFSNKSRNFRYGYRLVDGYSENALVEGKSTLESLHELARIHNINCVLTNTLYTVTQFNSYGTSSFEQDIKREL
NSAFMSLLGCAKNP

Specific function: De novo phospholipid biosynthesis; glycerol-3 phosphate formation. [C]

COG id: COG0240

COG function: function code C; Glycerol-3-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI33695088, Length=269, Percent_Identity=29.7397769516729, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI24307999, Length=265, Percent_Identity=27.1698113207547, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1790037, Length=304, Percent_Identity=31.5789473684211, Blast_Score=147, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17507425, Length=318, Percent_Identity=24.8427672955975, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI32564399, Length=270, Percent_Identity=30.7407407407407, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI32564403, Length=279, Percent_Identity=30.1075268817204, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI193210136, Length=279, Percent_Identity=30.1075268817204, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI193210134, Length=263, Percent_Identity=25.8555133079848, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324513, Length=305, Percent_Identity=30.4918032786885, Blast_Score=106, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6320181, Length=273, Percent_Identity=29.3040293040293, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI22026922, Length=278, Percent_Identity=28.0575539568345, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI17136204, Length=265, Percent_Identity=26.4150943396226, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17136200, Length=265, Percent_Identity=26.4150943396226, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17136202, Length=266, Percent_Identity=27.0676691729323, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45551945, Length=268, Percent_Identity=24.2537313432836, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281362270, Length=268, Percent_Identity=24.2537313432836, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPDA_NEOSM (Q2GEH4)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506122.1
- ProteinModelPortal:   Q2GEH4
- SMR:   Q2GEH4
- STRING:   Q2GEH4
- GeneID:   3932274
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0228
- TIGR:   NSE_0228
- eggNOG:   COG0240
- HOGENOM:   HBG586392
- OMA:   NVAKGIE
- PhylomeDB:   Q2GEH4
- ProtClustDB:   CLSK2527997
- BioCyc:   NSEN222891:NSE_0228-MONOMER
- HAMAP:   MF_00394
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR006168
- InterPro:   IPR006109
- InterPro:   IPR011128
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR11728
- PIRSF:   PIRSF000114
- PRINTS:   PR00077

Pfam domain/function: PF07479 NAD_Gly3P_dh_C; PF01210 NAD_Gly3P_dh_N; SSF48179 6DGDH_C_like

EC number: =1.1.1.94

Molecular weight: Translated: 37034; Mature: 37034

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00957 NAD_G3PDH

Important sites: ACT_SITE 190-190 BINDING 107-107 BINDING 107-107 BINDING 139-139 BINDING 254-254 BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLMDSVVIGGGAWGTAIANLLAFNTQRVTIFCRNTTVIDSINKRHINTKYLPTFPLNKN
CEEECCEEECCCHHHHHHHHHHHCCCCEEEEEECCCCHHHCCCHHCCCCCCCCCCCCCCC
ISATSRMDVLKNAELIFVAVPSQSMRELLQKVKENIKESVQIILCNKGIERESLLLMSEV
CCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHH
VHEELPKNDIFVLSGPNFAHEVLSKKPSFSNLAGRNKTSYDKIANALSTETFFTKYITDI
HHHHCCCCCEEEEECCCHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
NGTQILGAFKNVIAIICGLLVRMDAGSNTLSALMSLALEEARSFITIKNGNPDTIMEFCG
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHC
IGDLVLTCFSNKSRNFRYGYRLVDGYSENALVEGKSTLESLHELARIHNINCVLTNTLYT
CHHHHHHHHCCCCCCEEECEEEECCCCCCCEECCHHHHHHHHHHHHHHCCEEEEECCEEE
VTQFNSYGTSSFEQDIKRELNSAFMSLLGCAKNP
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVLMDSVVIGGGAWGTAIANLLAFNTQRVTIFCRNTTVIDSINKRHINTKYLPTFPLNKN
CEEECCEEECCCHHHHHHHHHHHCCCCEEEEEECCCCHHHCCCHHCCCCCCCCCCCCCCC
ISATSRMDVLKNAELIFVAVPSQSMRELLQKVKENIKESVQIILCNKGIERESLLLMSEV
CCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHH
VHEELPKNDIFVLSGPNFAHEVLSKKPSFSNLAGRNKTSYDKIANALSTETFFTKYITDI
HHHHCCCCCEEEEECCCHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
NGTQILGAFKNVIAIICGLLVRMDAGSNTLSALMSLALEEARSFITIKNGNPDTIMEFCG
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHC
IGDLVLTCFSNKSRNFRYGYRLVDGYSENALVEGKSTLESLHELARIHNINCVLTNTLYT
CHHHHHHHHCCCCCCEEECEEEECCCCCCCEECCHHHHHHHHHHHHHHCCEEEEECCEEE
VTQFNSYGTSSFEQDIKRELNSAFMSLLGCAKNP
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA