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The map label for this gene is greA

Identifier: 88608560

GI number: 88608560

Start: 585762

End: 586241

Strand: Reverse

Name: greA

Synonym: NSE_0669

Alternate gene names: 88608560

Gene position: 586241-585762 (Counterclockwise)

Preceding gene: 88608087

Following gene: 88608309

Centisome position: 68.25

GC content: 40.62

Gene sequence:

>480_bases
ATGAGAAAGTTACCTATCACTAGGGAGGGGTATTGTAAACTTCAGGAGGAACTAGAAAAGCTTGTCAAAGTGGTGAAGCC
GTCAGTTGTAGCCGCAGTTGCTCAGGCTAGAGAATTAGGTGATCTTTCTGAGAATGCTGAATACCATGAAGCACGAAAAG
AGCAAAGTTTTGTTGAAGGGAAAATACGGGAATTGCAGTTATGTCTTTCAGAGGCAGAAGTGATAGATGTTTCTCAATTT
ACAGGCGAAAGGGTAAAATTTGGTGCCTCCGTGACGCTAGAAAATATGGAAAGTAAGAGCGTGCTAATCTACCAAATTGT
AGGTAACCTCGAGTCTGATATAAAGGAAGGAAAAATATCTGTGTCCTCACCAATCGGAAAGGCAGTAATGAATAAGGAAG
TTGGTGAAATCGTGGAAATAGATCTCCCGAGTGGTAAGAAAACCTATAAAATCTTGGGTGTGGAATTTAGACAAAGTTGA

Upstream 100 bases:

>100_bases
GGAAATCCATGATTTGTGTACTCTTAGAGGATTTTGGTGTAGAATCACGGCGCTTGCTTTTATTTTGTTGGCATAATATT
TGTCTTTGGGAGGGTACAAG

Downstream 100 bases:

>100_bases
AGCGTCTCTTCGATACAAGAGCTATAATTGCTCGCAGGAAGCGTTTTGCGGGGGTGGATGCGTCCCATTATTATCTTTTA
CTGGAGATATATGCCCTTCT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MRKLPITREGYCKLQEELEKLVKVVKPSVVAAVAQARELGDLSENAEYHEARKEQSFVEGKIRELQLCLSEAEVIDVSQF
TGERVKFGASVTLENMESKSVLIYQIVGNLESDIKEGKISVSSPIGKAVMNKEVGEIVEIDLPSGKKTYKILGVEFRQS

Sequences:

>Translated_159_residues
MRKLPITREGYCKLQEELEKLVKVVKPSVVAAVAQARELGDLSENAEYHEARKEQSFVEGKIRELQLCLSEAEVIDVSQF
TGERVKFGASVTLENMESKSVLIYQIVGNLESDIKEGKISVSSPIGKAVMNKEVGEIVEIDLPSGKKTYKILGVEFRQS
>Mature_159_residues
MRKLPITREGYCKLQEELEKLVKVVKPSVVAAVAQARELGDLSENAEYHEARKEQSFVEGKIRELQLCLSEAEVIDVSQF
TGERVKFGASVTLENMESKSVLIYQIVGNLESDIKEGKISVSSPIGKAVMNKEVGEIVEIDLPSGKKTYKILGVEFRQS

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=45.2229299363057, Blast_Score=140, Evalue=3e-35,
Organism=Escherichia coli, GI87082261, Length=156, Percent_Identity=33.974358974359, Blast_Score=97, Evalue=6e-22,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_NEOSM (Q2GD99)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506547.1
- ProteinModelPortal:   Q2GD99
- SMR:   Q2GD99
- STRING:   Q2GD99
- GeneID:   3931739
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0669
- TIGR:   NSE_0669
- eggNOG:   COG0782
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- PhylomeDB:   Q2GD99
- ProtClustDB:   PRK00226
- BioCyc:   NSEN222891:NSE_0669-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17688; Mature: 17688

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLPITREGYCKLQEELEKLVKVVKPSVVAAVAQARELGDLSENAEYHEARKEQSFVEG
CCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
KIRELQLCLSEAEVIDVSQFTGERVKFGASVTLENMESKSVLIYQIVGNLESDIKEGKIS
HHHHHHHHHHHHHEEEHHHHCCCCEECCCEEEECCCCCCCEEEHHHHCCHHHHHHHCCEE
VSSPIGKAVMNKEVGEIVEIDLPSGKKTYKILGVEFRQS
CCCCHHHHHHHHHCCCEEEEECCCCCCEEEEEEEEECCC
>Mature Secondary Structure
MRKLPITREGYCKLQEELEKLVKVVKPSVVAAVAQARELGDLSENAEYHEARKEQSFVEG
CCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
KIRELQLCLSEAEVIDVSQFTGERVKFGASVTLENMESKSVLIYQIVGNLESDIKEGKIS
HHHHHHHHHHHHHEEEHHHHCCCCEECCCEEEECCCCCCCEEEHHHHCCHHHHHHHCCEE
VSSPIGKAVMNKEVGEIVEIDLPSGKKTYKILGVEFRQS
CCCCHHHHHHHHHCCCEEEEECCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA