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The map label for this gene is tyrS

Identifier: 88608544

GI number: 88608544

Start: 283091

End: 284266

Strand: Reverse

Name: tyrS

Synonym: NSE_0345

Alternate gene names: 88608544

Gene position: 284266-283091 (Counterclockwise)

Preceding gene: 88608645

Following gene: 88608168

Centisome position: 33.09

GC content: 40.65

Gene sequence:

>1176_bases
ATGCTGAAAATAATTGAGGAGAGAGGTTATTTTTTCCAGTGTACGGACAGAGAGGCATTAGAGCATCGTCTACATTCAGG
TGAAAAGCTTGTCTTCTATTTAGGTTTTGATTGCACAGCATCTGCTATACATCTGGGACATCTAGTACCGCTGATGCTGA
TGCGCTTGGTAAAGAGCTATGGACACGAAGTAATAATACTACTCGGCGGTGGTACTACTCTCATAGGAGATCCATCTGGA
AAAGACAAATCCCGCAAAATACTTTCCAGGGAAGAAATAAATAGGAATGTTGATACCATCAGCAAAATCGTGAAGCGGGT
TGTAGGAGATGTGCGGTTTGTTAACAATGTTGACTGGCTAGAAGACATTAGATACATAGATTTTCTCCGAAACGTGGGAC
GTCACTTTTCTATAAACAGAATGCTCACCTTTGATAGTGTGAAAAGTCGTTTAGATCGTGAGTCTCATCTTAGTTTTTTA
GAATTCAGTTACATGATACTTCAAGCTTACGATTTCGTTGAATTAAACCGCCTGTATAACTGCAGTGTACAAGTAGGTGG
AGGAGATCAGTGGGGCAACATAGTCAATGGTGTGGAATTGGGAAGAAAATTGAGAAATACACAGTTGTTTGGTTTGACCG
TACCATTGATCACAACCGCTTCAGGAAAAAAAATGGGCAAAACTGAAGAAGGAGCCGTCTGGTTAGATGCAGATCTATAC
TCGCCCCATGATTATTGGCAATATTTCCGGAATACTGAGGATTCGGATGTGGAAAGATTCTTACGACTTTTTACTGAGCT
ACCAATCGATGAGATAGAAACTCTTTCGGCGTGTGATGATGCTCAAATAAATGAGGCAAAAAAGGTGCTAGCCACCGAGG
CAACTAGAATATGTCACGGCAGTGTGATTGCAGAAGAGGTCAGCACAAGTATAATCAAGGCCTTTGAAGAAGATGATCTG
TCTCAGTTACCAGAAATAAGGGTTCCACATGGTGCAAACATAATCGATGTAGTCATTAGGATCGAAGCTGCTTCCTCTAG
AACTCAAGCAAAAAGATTGATAGAGTCAGGTGCAGTTAAAATAGATAAGAAAAAGATACTTACCTACAACCTCGACTTGC
CGGAGCAATTTGTTGTGAGTGTTGGAAAAAAACAGCGCAATATTATAGTGACATAA

Upstream 100 bases:

>100_bases
AAACACTTGGAGTGGTTGGAGCAGCAATTATCTTTAATTCAAAGCCTGGGAATCCAGCAGTACCTGGTGAAGCACGGTTG
AAGGCTGAGTTCTCTTGGTC

Downstream 100 bases:

>100_bases
CACACCTCCTGTGATAGATAGTGGGACCATGTCCATTGGATCCTCGCTTTATCTGCTTCTCACTGCGACGTGCGCCAGAA
TCTCCTCCCACTGCTGCCGG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MLKIIEERGYFFQCTDREALEHRLHSGEKLVFYLGFDCTASAIHLGHLVPLMLMRLVKSYGHEVIILLGGGTTLIGDPSG
KDKSRKILSREEINRNVDTISKIVKRVVGDVRFVNNVDWLEDIRYIDFLRNVGRHFSINRMLTFDSVKSRLDRESHLSFL
EFSYMILQAYDFVELNRLYNCSVQVGGGDQWGNIVNGVELGRKLRNTQLFGLTVPLITTASGKKMGKTEEGAVWLDADLY
SPHDYWQYFRNTEDSDVERFLRLFTELPIDEIETLSACDDAQINEAKKVLATEATRICHGSVIAEEVSTSIIKAFEEDDL
SQLPEIRVPHGANIIDVVIRIEAASSRTQAKRLIESGAVKIDKKKILTYNLDLPEQFVVSVGKKQRNIIVT

Sequences:

>Translated_391_residues
MLKIIEERGYFFQCTDREALEHRLHSGEKLVFYLGFDCTASAIHLGHLVPLMLMRLVKSYGHEVIILLGGGTTLIGDPSG
KDKSRKILSREEINRNVDTISKIVKRVVGDVRFVNNVDWLEDIRYIDFLRNVGRHFSINRMLTFDSVKSRLDRESHLSFL
EFSYMILQAYDFVELNRLYNCSVQVGGGDQWGNIVNGVELGRKLRNTQLFGLTVPLITTASGKKMGKTEEGAVWLDADLY
SPHDYWQYFRNTEDSDVERFLRLFTELPIDEIETLSACDDAQINEAKKVLATEATRICHGSVIAEEVSTSIIKAFEEDDL
SQLPEIRVPHGANIIDVVIRIEAASSRTQAKRLIESGAVKIDKKKILTYNLDLPEQFVVSVGKKQRNIIVT
>Mature_391_residues
MLKIIEERGYFFQCTDREALEHRLHSGEKLVFYLGFDCTASAIHLGHLVPLMLMRLVKSYGHEVIILLGGGTTLIGDPSG
KDKSRKILSREEINRNVDTISKIVKRVVGDVRFVNNVDWLEDIRYIDFLRNVGRHFSINRMLTFDSVKSRLDRESHLSFL
EFSYMILQAYDFVELNRLYNCSVQVGGGDQWGNIVNGVELGRKLRNTQLFGLTVPLITTASGKKMGKTEEGAVWLDADLY
SPHDYWQYFRNTEDSDVERFLRLFTELPIDEIETLSACDDAQINEAKKVLATEATRICHGSVIAEEVSTSIIKAFEEDDL
SQLPEIRVPHGANIIDVVIRIEAASSRTQAKRLIESGAVKIDKKKILTYNLDLPEQFVVSVGKKQRNIIVT

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=368, Percent_Identity=34.5108695652174, Blast_Score=205, Evalue=5e-53,
Organism=Escherichia coli, GI1787925, Length=386, Percent_Identity=42.4870466321244, Blast_Score=307, Evalue=8e-85,
Organism=Caenorhabditis elegans, GI25145802, Length=311, Percent_Identity=36.6559485530547, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25145805, Length=243, Percent_Identity=36.2139917695473, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6325160, Length=326, Percent_Identity=36.5030674846626, Blast_Score=221, Evalue=1e-58,
Organism=Drosophila melanogaster, GI20130383, Length=401, Percent_Identity=34.4139650872818, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44444; Mature: 44444

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIIEERGYFFQCTDREALEHRLHSGEKLVFYLGFDCTASAIHLGHLVPLMLMRLVKSY
CCEEEECCCCEEEECCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHC
GHEVIILLGGGTTLIGDPSGKDKSRKILSREEINRNVDTISKIVKRVVGDVRFVNNVDWL
CCEEEEEECCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHH
EDIRYIDFLRNVGRHFSINRMLTFDSVKSRLDRESHLSFLEFSYMILQAYDFVELNRLYN
HHHHHHHHHHHHCCCEEECCEEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHEEE
CSVQVGGGDQWGNIVNGVELGRKLRNTQLFGLTVPLITTASGKKMGKTEEGAVWLDADLY
EEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCCCCCCCCCCEEEECCCC
SPHDYWQYFRNTEDSDVERFLRLFTELPIDEIETLSACDDAQINEAKKVLATEATRICHG
CCHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
SVIAEEVSTSIIKAFEEDDLSQLPEIRVPHGANIIDVVIRIEAASSRTQAKRLIESGAVK
HHHHHHHHHHHHHHHHHCHHHHCCCEECCCCCCEEEEEEEEECCCCHHHHHHHHHHCCEE
IDKKKILTYNLDLPEQFVVSVGKKQRNIIVT
EECEEEEEEECCCCHHHHHHHCCCCCCEECC
>Mature Secondary Structure
MLKIIEERGYFFQCTDREALEHRLHSGEKLVFYLGFDCTASAIHLGHLVPLMLMRLVKSY
CCEEEECCCCEEEECCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHC
GHEVIILLGGGTTLIGDPSGKDKSRKILSREEINRNVDTISKIVKRVVGDVRFVNNVDWL
CCEEEEEECCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHH
EDIRYIDFLRNVGRHFSINRMLTFDSVKSRLDRESHLSFLEFSYMILQAYDFVELNRLYN
HHHHHHHHHHHHCCCEEECCEEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHEEE
CSVQVGGGDQWGNIVNGVELGRKLRNTQLFGLTVPLITTASGKKMGKTEEGAVWLDADLY
EEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCCCCCCCCCCEEEECCCC
SPHDYWQYFRNTEDSDVERFLRLFTELPIDEIETLSACDDAQINEAKKVLATEATRICHG
CCHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
SVIAEEVSTSIIKAFEEDDLSQLPEIRVPHGANIIDVVIRIEAASSRTQAKRLIESGAVK
HHHHHHHHHHHHHHHHHCHHHHCCCEECCCCCCEEEEEEEEECCCCHHHHHHHHHHCCEE
IDKKKILTYNLDLPEQFVVSVGKKQRNIIVT
EECEEEEEEECCCCHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA