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The map label for this gene is prs

Identifier: 88608481

GI number: 88608481

Start: 726974

End: 727891

Strand: Reverse

Name: prs

Synonym: NSE_0816

Alternate gene names: 88608481

Gene position: 727891-726974 (Counterclockwise)

Preceding gene: 88608783

Following gene: 88608321

Centisome position: 84.74

GC content: 44.77

Gene sequence:

>918_bases
ATGAAAGTCCTCATGGGTAGCAAAGCTGGGAAACTTGGCCAGGAAATAGCGTCGCTCCTTAACCTGGCTGTGGTTCGTGC
ACAGGCAAATAGATTTCCTGATGGCGAAAGTGATGTTCTTGTGCACCATGAAGATGTTATTCCTGGAACTAAGGTGCTCG
TCATCCAGAGTCTTTGTAGTGATGCTGACATAATGGAATTTTTATTCATCGTGGATGCTTTGAACAGACTAGGGGTACGT
GATATTGCTTTAGTCTCCCCATATTTTGGTTATGCTCGTGCCGATAGGATTGTCGCGCCTGGCTCTGCCATTTCAGCAAA
AGTAGTTGCACGCCTTATTGGAGAGAGGATTTCATTGCTTTTTGTCTTAGATGTACACTTTCCTCAATTTGAGGGTTTCT
TCGAGATCCCAGTGTACAACATTCTTCCGTGGACGCTGCTTAAGGAAAGTGTGCATGTGGGTCACAATATGGCCCTTGTT
GCTCCCGATGTGGGTGCTGTAAAAAAGTTAAAGCCTCTCTCCGAAGAGCTGGGCACAAATCTTGTTATCATGAATAAACG
CAGGCCCAGAGCATGTCTATCCGAGGTTACTGAGGTGGTTGGGGAAATTCATGGTAGAGACTGCATAATAGTTGATGATA
TCGTCTGTGGTGGGGGGACCCTCTGCAATTCGGCTGCTAAACTTAAGGAGCTTGGTGCAAAATCTGTTGCTGCGTTTGTT
ACCCACGGCGTATTTTCTGGTAGCGCAAGACAGAAAATTATGGACTCACAGATAGATAGGATTGTTGTCACTAACAGCAT
ACCTGGACCTGGGTGTGAGGCTAAAGGGAAAATAGAGATCATTAGTGCTGCAGACTTGCTTGCAAGAAAAATTCATTCAG
TTTACCTAGATGTCAATACAAAAAGTCCTAATTGCTAA

Upstream 100 bases:

>100_bases
GTTGCCGTTTCTTTGAGAACGTGACCCCTTGTGGTGGATTTGTTTTACCGTATTCGATATCCTTTTCTAATAAATTCAAC
GTGGCTGTTGTGCTAATAAC

Downstream 100 bases:

>100_bases
TCGCGGGGAGATAGTATCAAGGATAGCACGTACCTTAAAAAAATTGGGTAAGAAGAGTGTTGCTATTTATTCCGATCTCG
ATGTTAATGCGGAGTATATT

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKVLMGSKAGKLGQEIASLLNLAVVRAQANRFPDGESDVLVHHEDVIPGTKVLVIQSLCSDADIMEFLFIVDALNRLGVR
DIALVSPYFGYARADRIVAPGSAISAKVVARLIGERISLLFVLDVHFPQFEGFFEIPVYNILPWTLLKESVHVGHNMALV
APDVGAVKKLKPLSEELGTNLVIMNKRRPRACLSEVTEVVGEIHGRDCIIVDDIVCGGGTLCNSAAKLKELGAKSVAAFV
THGVFSGSARQKIMDSQIDRIVVTNSIPGPGCEAKGKIEIISAADLLARKIHSVYLDVNTKSPNC

Sequences:

>Translated_305_residues
MKVLMGSKAGKLGQEIASLLNLAVVRAQANRFPDGESDVLVHHEDVIPGTKVLVIQSLCSDADIMEFLFIVDALNRLGVR
DIALVSPYFGYARADRIVAPGSAISAKVVARLIGERISLLFVLDVHFPQFEGFFEIPVYNILPWTLLKESVHVGHNMALV
APDVGAVKKLKPLSEELGTNLVIMNKRRPRACLSEVTEVVGEIHGRDCIIVDDIVCGGGTLCNSAAKLKELGAKSVAAFV
THGVFSGSARQKIMDSQIDRIVVTNSIPGPGCEAKGKIEIISAADLLARKIHSVYLDVNTKSPNC
>Mature_305_residues
MKVLMGSKAGKLGQEIASLLNLAVVRAQANRFPDGESDVLVHHEDVIPGTKVLVIQSLCSDADIMEFLFIVDALNRLGVR
DIALVSPYFGYARADRIVAPGSAISAKVVARLIGERISLLFVLDVHFPQFEGFFEIPVYNILPWTLLKESVHVGHNMALV
APDVGAVKKLKPLSEELGTNLVIMNKRRPRACLSEVTEVVGEIHGRDCIIVDDIVCGGGTLCNSAAKLKELGAKSVAAFV
THGVFSGSARQKIMDSQIDRIVVTNSIPGPGCEAKGKIEIISAADLLARKIHSVYLDVNTKSPNC

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=305, Percent_Identity=30.8196721311475, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI28557709, Length=305, Percent_Identity=30.8196721311475, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI4506129, Length=300, Percent_Identity=31, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI84875539, Length=303, Percent_Identity=31.023102310231, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI4506133, Length=300, Percent_Identity=27.6666666666667, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787458, Length=275, Percent_Identity=39.2727272727273, Blast_Score=192, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17554702, Length=303, Percent_Identity=33.003300330033, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25149168, Length=303, Percent_Identity=33.003300330033, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71989924, Length=303, Percent_Identity=33.003300330033, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17554704, Length=301, Percent_Identity=32.890365448505, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320946, Length=276, Percent_Identity=32.9710144927536, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319403, Length=279, Percent_Identity=32.9749103942652, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321776, Length=293, Percent_Identity=30.7167235494881, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324511, Length=74, Percent_Identity=41.8918918918919, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322667, Length=203, Percent_Identity=27.0935960591133, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21355239, Length=280, Percent_Identity=30.3571428571429, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI45551540, Length=306, Percent_Identity=28.7581699346405, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281362873, Length=315, Percent_Identity=26.031746031746, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24651454, Length=315, Percent_Identity=26.031746031746, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24651458, Length=315, Percent_Identity=26.031746031746, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24651456, Length=315, Percent_Identity=26.031746031746, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45552010, Length=171, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24651462, Length=171, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24651464, Length=171, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 32845; Mature: 32845

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLMGSKAGKLGQEIASLLNLAVVRAQANRFPDGESDVLVHHEDVIPGTKVLVIQSLCS
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHC
DADIMEFLFIVDALNRLGVRDIALVSPYFGYARADRIVAPGSAISAKVVARLIGERISLL
CHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHCCCEECCCCHHHHHHHHHHHHHHEEEE
FVLDVHFPQFEGFFEIPVYNILPWTLLKESVHVGHNMALVAPDVGAVKKLKPLSEELGTN
EEEEECCCCCCCEEECCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCC
LVIMNKRRPRACLSEVTEVVGEIHGRDCIIVDDIVCGGGTLCNSAAKLKELGAKSVAAFV
EEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECEEECCCHHHHHHHHHHHHHHHHHHHHH
THGVFSGSARQKIMDSQIDRIVVTNSIPGPGCEAKGKIEIISAADLLARKIHSVYLDVNT
HHHHCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHEEEEEEC
KSPNC
CCCCC
>Mature Secondary Structure
MKVLMGSKAGKLGQEIASLLNLAVVRAQANRFPDGESDVLVHHEDVIPGTKVLVIQSLCS
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHC
DADIMEFLFIVDALNRLGVRDIALVSPYFGYARADRIVAPGSAISAKVVARLIGERISLL
CHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHCCCEECCCCHHHHHHHHHHHHHHEEEE
FVLDVHFPQFEGFFEIPVYNILPWTLLKESVHVGHNMALVAPDVGAVKKLKPLSEELGTN
EEEEECCCCCCCEEECCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCC
LVIMNKRRPRACLSEVTEVVGEIHGRDCIIVDDIVCGGGTLCNSAAKLKELGAKSVAAFV
EEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECEEECCCHHHHHHHHHHHHHHHHHHHHH
THGVFSGSARQKIMDSQIDRIVVTNSIPGPGCEAKGKIEIISAADLLARKIHSVYLDVNT
HHHHCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHEEEEEEC
KSPNC
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]