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The map label for this gene is pccA

Identifier: 88608321

GI number: 88608321

Start: 725044

End: 727002

Strand: Reverse

Name: pccA

Synonym: NSE_0815

Alternate gene names: 88608321

Gene position: 727002-725044 (Counterclockwise)

Preceding gene: 88608481

Following gene: 88608260

Centisome position: 84.63

GC content: 41.7

Gene sequence:

>1959_bases
ATGTCAATACAAAAAGTCCTAATTGCTAATCGCGGGGAGATAGTATCAAGGATAGCACGTACCTTAAAAAAATTGGGTAA
GAAGAGTGTTGCTATTTATTCCGATCTCGATGTTAATGCGGAGTATATTCGGCATACAGATGAAGCAATCTACATAGGGG
GTGTTACCGTTAGTGAGAGCTATAACAATATGGAATCCATACTCACAGCGGTCAAAAAAAGTGGGGCAGATGCTGTACAT
CCTGGGTATGGGTTTTTGTCTGAAAATCCTAGTTTTGCCCGATCTGTTGAGCAAGCTGGGTTTACCTTTATTGGTCCTAG
CTCAGAGTGCATCTCCATGATGAGTGATAAGGTGATGGCAAAAAAGATTGCTCAGGAGGCCTCAATCAATCTTGTACCCG
GGTATTTAGGGGAAATCTCTGATTATGAACAAGCTGTCTCTATTGCGAAAGAAATAGGTTTCCCAGTTCTGATAAAGGCT
GCCGCCGGAGGGGGAGGTAAAGGAATGCGGTTGGTCAATAGAGAAGAGGAGTTACGCGAAGCTATGCAGCTTGCTTGTCA
TGAGGCTCTGAATTTCTTTGCTGATGAACGAATTTTCATTGAGAAATATATAACGAACCCCAGACATATAGAAATACAGC
TGATTGCTGACAAAAATGGCCACGTTATTTGTTTGGGTGAGCGAGAATGTTCGGTACAGAGGCGGCATCAAAAGGTTGTT
GAGGAGGCTCCCAGTCTTGCACTTGATCGGGGACTCAGAGAAAAGATGTATGAACAGTCTATTAGATTAGCCCGTGCAGC
TGGTTACACTTCTGTTGGGACGGTGGAATATATACTAGACCAGGAGCGAAATTTCTACTTTATGGAAATGAATGCTCGCA
TCCAAGTTGAACACCCGGTAACCGAAATGATTACTGATATTGATATAGTCGAAGAGATGATCCGCATCGCTGAGGGCGAT
GATCTATCAAAAAAGAAAAGTGTTACGAAGTTTAACGGTTGGTCATTTGAAGCACGCATATATGCAGAGGATCCCATGAA
CAACTTTATGCCAACGAGTGGTTTCGTATGTTGTGCTGATTTTCCTACTGATGTCAGGGTTGAAAGTAGTGTAGGTACGG
GCTCCAAGGTTGGTATGTTTTATGATCCAATGATGGCAAAGATTATCGTGAAGGGTAGAACGCGTTCTGATGCGTTAGAA
AGAATGTGTGAGGCGCTTCAGATGACGTGCATAGTGGGTGTAAAGAATAATTTAGTTTTTTTAGAATCCCTGTTTCGTAA
TGCTGATTTTATTAATGGAAATATAAGCACCCATTTTATAGAAAAGCAGTACCAAGGGGTAAGTTGGTATGAGTCTCTCG
ATAAATGTGCGGCCCTGCGCTTTATTGCAGTGACTTTATATGCCATGTTAGACATATGGAGTGGTTCGTTTGAAGAAAGT
CGTCCCTTTATTGTAAGACTTGGGAGTCAGGAATATGAAGTTGATGTCTCTATTGCTATGGGTACATTTAGCTTCACTCT
TGCTAAGGAGCGATATATTGTTGAGTCTCTGAGAGCAACAAAAGAAGGCCTGTTTCTTGCAAAGATCAATGATGAAGCGT
TTGCCGCGAGGTTCTTCAACACTGATCAATTATACAAACGAGTTGAGTACGATGGTTTTTCATTGCTATGTATTGCTCTT
CCTAAGGAAGTGTATACGAAGTGGGTATCCGTACAAAAGAATGCTTCAGGTATACAACAATCAGATAAAATTATGTCTGC
AATGACAGGGGTCGTAGTAAGCATTTACGTTAGTGTTGGTGACATGATCAGACCAGGTGATCCGTTGTTTTTAGTCGAAG
CAATGAAAATGCAGAACACCATCACTGCCGAACGAGCTGGTACTGTGAAGGAAGTCTGCGTGATGCACGGTCAAAATGTA
AGCGAGGGTGATTTATTATTGAAGTTTTTAAAAGTATAA

Upstream 100 bases:

>100_bases
ACTAACAGCATACCTGGACCTGGGTGTGAGGCTAAAGGGAAAATAGAGATCATTAGTGCTGCAGACTTGCTTGCAAGAAA
AATTCATTCAGTTTACCTAG

Downstream 100 bases:

>100_bases
GTAAAGATCTGGATTTTATTTAGCTCCAAAAGCGGTTAATCTAATGATAAACGATTATGGGGTTCTTCTACGATTTGTCC
CTGCGTGTGTAATTCCTGTA

Product: propionyl-CoA carboxylase subunit alpha

Products: NA

Alternate protein names: Acetyl-CoA carboxylase subunit A; ACC [H]

Number of amino acids: Translated: 652; Mature: 651

Protein sequence:

>652_residues
MSIQKVLIANRGEIVSRIARTLKKLGKKSVAIYSDLDVNAEYIRHTDEAIYIGGVTVSESYNNMESILTAVKKSGADAVH
PGYGFLSENPSFARSVEQAGFTFIGPSSECISMMSDKVMAKKIAQEASINLVPGYLGEISDYEQAVSIAKEIGFPVLIKA
AAGGGGKGMRLVNREEELREAMQLACHEALNFFADERIFIEKYITNPRHIEIQLIADKNGHVICLGERECSVQRRHQKVV
EEAPSLALDRGLREKMYEQSIRLARAAGYTSVGTVEYILDQERNFYFMEMNARIQVEHPVTEMITDIDIVEEMIRIAEGD
DLSKKKSVTKFNGWSFEARIYAEDPMNNFMPTSGFVCCADFPTDVRVESSVGTGSKVGMFYDPMMAKIIVKGRTRSDALE
RMCEALQMTCIVGVKNNLVFLESLFRNADFINGNISTHFIEKQYQGVSWYESLDKCAALRFIAVTLYAMLDIWSGSFEES
RPFIVRLGSQEYEVDVSIAMGTFSFTLAKERYIVESLRATKEGLFLAKINDEAFAARFFNTDQLYKRVEYDGFSLLCIAL
PKEVYTKWVSVQKNASGIQQSDKIMSAMTGVVVSIYVSVGDMIRPGDPLFLVEAMKMQNTITAERAGTVKEVCVMHGQNV
SEGDLLLKFLKV

Sequences:

>Translated_652_residues
MSIQKVLIANRGEIVSRIARTLKKLGKKSVAIYSDLDVNAEYIRHTDEAIYIGGVTVSESYNNMESILTAVKKSGADAVH
PGYGFLSENPSFARSVEQAGFTFIGPSSECISMMSDKVMAKKIAQEASINLVPGYLGEISDYEQAVSIAKEIGFPVLIKA
AAGGGGKGMRLVNREEELREAMQLACHEALNFFADERIFIEKYITNPRHIEIQLIADKNGHVICLGERECSVQRRHQKVV
EEAPSLALDRGLREKMYEQSIRLARAAGYTSVGTVEYILDQERNFYFMEMNARIQVEHPVTEMITDIDIVEEMIRIAEGD
DLSKKKSVTKFNGWSFEARIYAEDPMNNFMPTSGFVCCADFPTDVRVESSVGTGSKVGMFYDPMMAKIIVKGRTRSDALE
RMCEALQMTCIVGVKNNLVFLESLFRNADFINGNISTHFIEKQYQGVSWYESLDKCAALRFIAVTLYAMLDIWSGSFEES
RPFIVRLGSQEYEVDVSIAMGTFSFTLAKERYIVESLRATKEGLFLAKINDEAFAARFFNTDQLYKRVEYDGFSLLCIAL
PKEVYTKWVSVQKNASGIQQSDKIMSAMTGVVVSIYVSVGDMIRPGDPLFLVEAMKMQNTITAERAGTVKEVCVMHGQNV
SEGDLLLKFLKV
>Mature_651_residues
SIQKVLIANRGEIVSRIARTLKKLGKKSVAIYSDLDVNAEYIRHTDEAIYIGGVTVSESYNNMESILTAVKKSGADAVHP
GYGFLSENPSFARSVEQAGFTFIGPSSECISMMSDKVMAKKIAQEASINLVPGYLGEISDYEQAVSIAKEIGFPVLIKAA
AGGGGKGMRLVNREEELREAMQLACHEALNFFADERIFIEKYITNPRHIEIQLIADKNGHVICLGERECSVQRRHQKVVE
EAPSLALDRGLREKMYEQSIRLARAAGYTSVGTVEYILDQERNFYFMEMNARIQVEHPVTEMITDIDIVEEMIRIAEGDD
LSKKKSVTKFNGWSFEARIYAEDPMNNFMPTSGFVCCADFPTDVRVESSVGTGSKVGMFYDPMMAKIIVKGRTRSDALER
MCEALQMTCIVGVKNNLVFLESLFRNADFINGNISTHFIEKQYQGVSWYESLDKCAALRFIAVTLYAMLDIWSGSFEESR
PFIVRLGSQEYEVDVSIAMGTFSFTLAKERYIVESLRATKEGLFLAKINDEAFAARFFNTDQLYKRVEYDGFSLLCIALP
KEVYTKWVSVQKNASGIQQSDKIMSAMTGVVVSIYVSVGDMIRPGDPLFLVEAMKMQNTITAERAGTVKEVCVMHGQNVS
EGDLLLKFLKV

Specific function: This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA [H]

COG id: COG4770

COG function: function code I; Acetyl/propionyl-CoA carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI189095269, Length=679, Percent_Identity=41.5316642120766, Blast_Score=496, Evalue=1e-140,
Organism=Homo sapiens, GI65506442, Length=679, Percent_Identity=41.5316642120766, Blast_Score=495, Evalue=1e-140,
Organism=Homo sapiens, GI295821183, Length=655, Percent_Identity=41.5267175572519, Blast_Score=491, Evalue=1e-138,
Organism=Homo sapiens, GI116805327, Length=680, Percent_Identity=38.0882352941176, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI106049528, Length=448, Percent_Identity=41.2946428571429, Blast_Score=346, Evalue=4e-95,
Organism=Homo sapiens, GI106049295, Length=448, Percent_Identity=41.2946428571429, Blast_Score=346, Evalue=4e-95,
Organism=Homo sapiens, GI106049292, Length=448, Percent_Identity=41.2946428571429, Blast_Score=346, Evalue=4e-95,
Organism=Homo sapiens, GI38679977, Length=505, Percent_Identity=31.6831683168317, Blast_Score=243, Evalue=4e-64,
Organism=Homo sapiens, GI38679967, Length=505, Percent_Identity=31.6831683168317, Blast_Score=243, Evalue=4e-64,
Organism=Homo sapiens, GI38679971, Length=505, Percent_Identity=31.6831683168317, Blast_Score=243, Evalue=5e-64,
Organism=Homo sapiens, GI38679974, Length=505, Percent_Identity=31.6831683168317, Blast_Score=243, Evalue=5e-64,
Organism=Homo sapiens, GI38679960, Length=505, Percent_Identity=31.6831683168317, Blast_Score=243, Evalue=5e-64,
Organism=Homo sapiens, GI134142062, Length=502, Percent_Identity=31.2749003984064, Blast_Score=239, Evalue=6e-63,
Organism=Escherichia coli, GI1789654, Length=444, Percent_Identity=43.9189189189189, Blast_Score=350, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17567343, Length=675, Percent_Identity=38.962962962963, Blast_Score=470, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI71987519, Length=661, Percent_Identity=37.3676248108926, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17562816, Length=462, Percent_Identity=42.8571428571429, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71997168, Length=507, Percent_Identity=31.5581854043393, Blast_Score=245, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI71997163, Length=507, Percent_Identity=31.5581854043393, Blast_Score=245, Evalue=7e-65,
Organism=Caenorhabditis elegans, GI133931226, Length=453, Percent_Identity=29.8013245033113, Blast_Score=205, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6319685, Length=505, Percent_Identity=40, Blast_Score=382, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6319695, Length=451, Percent_Identity=41.6851441241685, Blast_Score=311, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6321376, Length=465, Percent_Identity=41.0752688172043, Blast_Score=304, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6323863, Length=685, Percent_Identity=28.6131386861314, Blast_Score=249, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6324343, Length=729, Percent_Identity=28.1207133058985, Blast_Score=248, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24651757, Length=682, Percent_Identity=35.6304985337243, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24651759, Length=643, Percent_Identity=34.6811819595645, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24652212, Length=467, Percent_Identity=43.254817987152, Blast_Score=350, Evalue=2e-96,
Organism=Drosophila melanogaster, GI24652210, Length=467, Percent_Identity=43.254817987152, Blast_Score=350, Evalue=2e-96,
Organism=Drosophila melanogaster, GI24652214, Length=467, Percent_Identity=43.254817987152, Blast_Score=350, Evalue=2e-96,
Organism=Drosophila melanogaster, GI19921944, Length=467, Percent_Identity=43.254817987152, Blast_Score=350, Evalue=2e-96,
Organism=Drosophila melanogaster, GI24652216, Length=467, Percent_Identity=43.254817987152, Blast_Score=350, Evalue=2e-96,
Organism=Drosophila melanogaster, GI281363050, Length=467, Percent_Identity=43.254817987152, Blast_Score=350, Evalue=2e-96,
Organism=Drosophila melanogaster, GI24652224, Length=467, Percent_Identity=43.254817987152, Blast_Score=350, Evalue=2e-96,
Organism=Drosophila melanogaster, GI24652222, Length=467, Percent_Identity=43.254817987152, Blast_Score=350, Evalue=2e-96,
Organism=Drosophila melanogaster, GI24652220, Length=467, Percent_Identity=43.254817987152, Blast_Score=350, Evalue=2e-96,
Organism=Drosophila melanogaster, GI24652218, Length=467, Percent_Identity=43.254817987152, Blast_Score=350, Evalue=2e-96,
Organism=Drosophila melanogaster, GI161076407, Length=708, Percent_Identity=28.6723163841808, Blast_Score=249, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24586460, Length=708, Percent_Identity=28.6723163841808, Blast_Score=249, Evalue=5e-66,
Organism=Drosophila melanogaster, GI161076409, Length=708, Percent_Identity=28.6723163841808, Blast_Score=249, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24586458, Length=708, Percent_Identity=28.6723163841808, Blast_Score=248, Evalue=6e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.3.4.14; =6.4.1.2 [H]

Molecular weight: Translated: 72945; Mature: 72814

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQKVLIANRGEIVSRIARTLKKLGKKSVAIYSDLDVNAEYIRHTDEAIYIGGVTVSES
CCCCCEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCEEEEECEEEECC
YNNMESILTAVKKSGADAVHPGYGFLSENPSFARSVEQAGFTFIGPSSECISMMSDKVMA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHH
KKIAQEASINLVPGYLGEISDYEQAVSIAKEIGFPVLIKAAAGGGGKGMRLVNREEELRE
HHHHHHCCCEECCCHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEECCHHHHHHH
AMQLACHEALNFFADERIFIEKYITNPRHIEIQLIADKNGHVICLGERECSVQRRHQKVV
HHHHHHHHHHHHHCCCCEEEHHHCCCCCEEEEEEEECCCCCEEEEECCHHHHHHHHHHHH
EEAPSLALDRGLREKMYEQSIRLARAAGYTSVGTVEYILDQERNFYFMEMNARIQVEHPV
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCEEEEEECCEEEECCCH
TEMITDIDIVEEMIRIAEGDDLSKKKSVTKFNGWSFEARIYAEDPMNNFMPTSGFVCCAD
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCEECCCEEEEEEEECCCCCCCCCCCCEEEEEC
FPTDVRVESSVGTGSKVGMFYDPMMAKIIVKGRTRSDALERMCEALQMTCIVGVKNNLVF
CCCCEEEECCCCCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCHH
LESLFRNADFINGNISTHFIEKQYQGVSWYESLDKCAALRFIAVTLYAMLDIWSGSFEES
HHHHHCCCCEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RPFIVRLGSQEYEVDVSIAMGTFSFTLAKERYIVESLRATKEGLFLAKINDEAFAARFFN
CCEEEEECCCCEEEEEEEEEEEEEEEHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHCC
TDQLYKRVEYDGFSLLCIALPKEVYTKWVSVQKNASGIQQSDKIMSAMTGVVVSIYVSVG
HHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
DMIRPGDPLFLVEAMKMQNTITAERAGTVKEVCVMHGQNVSEGDLLLKFLKV
CCCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SIQKVLIANRGEIVSRIARTLKKLGKKSVAIYSDLDVNAEYIRHTDEAIYIGGVTVSES
CCCCEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCEEEEECEEEECC
YNNMESILTAVKKSGADAVHPGYGFLSENPSFARSVEQAGFTFIGPSSECISMMSDKVMA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHH
KKIAQEASINLVPGYLGEISDYEQAVSIAKEIGFPVLIKAAAGGGGKGMRLVNREEELRE
HHHHHHCCCEECCCHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEECCHHHHHHH
AMQLACHEALNFFADERIFIEKYITNPRHIEIQLIADKNGHVICLGERECSVQRRHQKVV
HHHHHHHHHHHHHCCCCEEEHHHCCCCCEEEEEEEECCCCCEEEEECCHHHHHHHHHHHH
EEAPSLALDRGLREKMYEQSIRLARAAGYTSVGTVEYILDQERNFYFMEMNARIQVEHPV
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCEEEEEECCEEEECCCH
TEMITDIDIVEEMIRIAEGDDLSKKKSVTKFNGWSFEARIYAEDPMNNFMPTSGFVCCAD
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCEECCCEEEEEEEECCCCCCCCCCCCEEEEEC
FPTDVRVESSVGTGSKVGMFYDPMMAKIIVKGRTRSDALERMCEALQMTCIVGVKNNLVF
CCCCEEEECCCCCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCHH
LESLFRNADFINGNISTHFIEKQYQGVSWYESLDKCAALRFIAVTLYAMLDIWSGSFEES
HHHHHCCCCEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RPFIVRLGSQEYEVDVSIAMGTFSFTLAKERYIVESLRATKEGLFLAKINDEAFAARFFN
CCEEEEECCCCEEEEEEEEEEEEEEEHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHCC
TDQLYKRVEYDGFSLLCIALPKEVYTKWVSVQKNASGIQQSDKIMSAMTGVVVSIYVSVG
HHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
DMIRPGDPLFLVEAMKMQNTITAERAGTVKEVCVMHGQNVSEGDLLLKFLKV
CCCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]