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The map label for this gene is gatB

Identifier: 88608306

GI number: 88608306

Start: 72068

End: 73534

Strand: Direct

Name: gatB

Synonym: NSE_0084

Alternate gene names: 88608306

Gene position: 72068-73534 (Clockwise)

Preceding gene: 88608259

Following gene: 88608086

Centisome position: 8.39

GC content: 43.08

Gene sequence:

>1467_bases
ATGTATTATATAGATGGAAAGACTGCTCGATGGGAGGTTGTTATCGGACTGGAGGTACATGCACAGATTCTCTCCAAAAA
TAAATTATTCTCCGATGCTCCATGTGATTCTACTAAGGGGCCTAATACGGCAGTGACTCTTTTTGATGCTGCAATGCCAG
GAGTCCTGCCAGTTTTGAATTTTAACTGTGTTGAGAAAGCTATTAGAGCTGGTTTAGGTCTTGGAGGGAAGATTAATTTA
TACTCGGTATTCGAGAGGAAACACTACTTTTATCCAGATCTCCCGCATGGTTACCAGATTACCCAAAATAGCTATCCGAT
CATAACTGGTGGGTGTGTGCGAATAAGAAATCCTGATAAAGTCATTAGGATAAATCGCATACACATCGAACAGGATGCAG
GCAAAAGCATACATAACCTCGCACCTGGCAAAACATATATAGACCTTAATAGGGCCGGAATCCCATTGATGGAGATAGTC
TCAGAGCCCGATATATCATCTCCTGCTGAGGCAGTCGCTTATGTGGACAAGTTGAAGCTTATTCTGCAGTATGTTTGTGC
TTCGAACGCTAACATGGAAAAAGGTGAGCTTCGCTGTGATGCAAACGTTTCGGTTCGAAGGGGAGGTGAGTCGGGGCTGG
GTACAAGATGTGAGATAAAAAATCTCAACTCGACTAAAAGCCTGGCTCAGGCGATAGAACATGAAGCTAAAAGACAAGTA
GAAGTTCTTGAAAGTGGTGGCACGATTGTGGTCGAAACAAAACTTTTTGATGTGGACACTCTAAGTACTATTGCCACAAG
ATCCAAGGAATCTGCAACCGAATACCGTTATTTTGCGGATCCTGACTTGCTTCCTCTTGTTTTGGAGGAAAAGTATGTAG
AGGATATTAAGGCTTCGATGCCTGAGTTACCCGACGAGAGAGAAAATAGATATGTGAGAGAGCTTAACTTATCTCCATAT
GATGCGAACGTGCTTGTTTCGAATGTTAATGCCTCGCGATATTTTGATGCCCTTTTGGAACTTGGTCATCAGCCAAAACT
TGCTGCTAGATGGATCACTGTTGAGCTCTTCGGTTTGCTTAACAAGCGTGGTATCAAAATCGTGGAAAGTCCTGTGACTC
CAGCTTTGATGGATGAACTTCTGAGACTTGTGCATAGCGCGGAAATATCAGAGCGAACGGCAAAAGAAGTGCTACGAAAA
AGCTTTGAAGGTATGGGTTCGCCGAAAGAAATTGTGGAACGCGAGAGCTTACGACAAGTCACGGACGATAAGGTTATTCT
CGGGTATGTAAAAGAGGTATTAGATGAAAATCCTACGAAGCTTGGAGAGTATTTCATGGGTAAAGAGAAAATGCTTGCCT
TTTTGGTTGGGCAAGTGATTAAGAAAAGCAACGGGAATGCAAGTCCCCATTTGGTAAACAAAGTTTTACTTGAGGAACTC
GAAAGGCGTCGCGCACAGAATGGATAA

Upstream 100 bases:

>100_bases
TGGACGTAATACCTGCATTTTATACATTCATAAGGTGAAAGGGGGTGTACCTTTGGTCGTGGTTTGCATAGAATGATCCA
CTGGGAGGTCGTTTGAGGTC

Downstream 100 bases:

>100_bases
GAAGTTCATGGAGCTAGCAATGAGTGTTGCTCGTCAGTCTGATTGTGAAGTGCCTGTTGGGGCGGTCCTAGTTAGGCAGG
GTGCTGTTATTGCCTCATCT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MYYIDGKTARWEVVIGLEVHAQILSKNKLFSDAPCDSTKGPNTAVTLFDAAMPGVLPVLNFNCVEKAIRAGLGLGGKINL
YSVFERKHYFYPDLPHGYQITQNSYPIITGGCVRIRNPDKVIRINRIHIEQDAGKSIHNLAPGKTYIDLNRAGIPLMEIV
SEPDISSPAEAVAYVDKLKLILQYVCASNANMEKGELRCDANVSVRRGGESGLGTRCEIKNLNSTKSLAQAIEHEAKRQV
EVLESGGTIVVETKLFDVDTLSTIATRSKESATEYRYFADPDLLPLVLEEKYVEDIKASMPELPDERENRYVRELNLSPY
DANVLVSNVNASRYFDALLELGHQPKLAARWITVELFGLLNKRGIKIVESPVTPALMDELLRLVHSAEISERTAKEVLRK
SFEGMGSPKEIVERESLRQVTDDKVILGYVKEVLDENPTKLGEYFMGKEKMLAFLVGQVIKKSNGNASPHLVNKVLLEEL
ERRRAQNG

Sequences:

>Translated_488_residues
MYYIDGKTARWEVVIGLEVHAQILSKNKLFSDAPCDSTKGPNTAVTLFDAAMPGVLPVLNFNCVEKAIRAGLGLGGKINL
YSVFERKHYFYPDLPHGYQITQNSYPIITGGCVRIRNPDKVIRINRIHIEQDAGKSIHNLAPGKTYIDLNRAGIPLMEIV
SEPDISSPAEAVAYVDKLKLILQYVCASNANMEKGELRCDANVSVRRGGESGLGTRCEIKNLNSTKSLAQAIEHEAKRQV
EVLESGGTIVVETKLFDVDTLSTIATRSKESATEYRYFADPDLLPLVLEEKYVEDIKASMPELPDERENRYVRELNLSPY
DANVLVSNVNASRYFDALLELGHQPKLAARWITVELFGLLNKRGIKIVESPVTPALMDELLRLVHSAEISERTAKEVLRK
SFEGMGSPKEIVERESLRQVTDDKVILGYVKEVLDENPTKLGEYFMGKEKMLAFLVGQVIKKSNGNASPHLVNKVLLEEL
ERRRAQNG
>Mature_488_residues
MYYIDGKTARWEVVIGLEVHAQILSKNKLFSDAPCDSTKGPNTAVTLFDAAMPGVLPVLNFNCVEKAIRAGLGLGGKINL
YSVFERKHYFYPDLPHGYQITQNSYPIITGGCVRIRNPDKVIRINRIHIEQDAGKSIHNLAPGKTYIDLNRAGIPLMEIV
SEPDISSPAEAVAYVDKLKLILQYVCASNANMEKGELRCDANVSVRRGGESGLGTRCEIKNLNSTKSLAQAIEHEAKRQV
EVLESGGTIVVETKLFDVDTLSTIATRSKESATEYRYFADPDLLPLVLEEKYVEDIKASMPELPDERENRYVRELNLSPY
DANVLVSNVNASRYFDALLELGHQPKLAARWITVELFGLLNKRGIKIVESPVTPALMDELLRLVHSAEISERTAKEVLRK
SFEGMGSPKEIVERESLRQVTDDKVILGYVKEVLDENPTKLGEYFMGKEKMLAFLVGQVIKKSNGNASPHLVNKVLLEEL
ERRRAQNG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=38.1818181818182, Blast_Score=321, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI115534094, Length=303, Percent_Identity=39.6039603960396, Blast_Score=212, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=502, Percent_Identity=32.0717131474104, Blast_Score=232, Evalue=1e-61,
Organism=Drosophila melanogaster, GI45550808, Length=489, Percent_Identity=34.560327198364, Blast_Score=278, Evalue=5e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_NEOSM (Q2GEW1)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_505985.1
- ProteinModelPortal:   Q2GEW1
- SMR:   Q2GEW1
- STRING:   Q2GEW1
- GeneID:   3932268
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0084
- TIGR:   NSE_0084
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q2GEW1
- ProtClustDB:   PRK05477
- BioCyc:   NSEN222891:NSE_0084-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54471; Mature: 54471

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYIDGKTARWEVVIGLEVHAQILSKNKLFSDAPCDSTKGPNTAVTLFDAAMPGVLPVLN
CEEECCCCEEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHCCCHHHHCC
FNCVEKAIRAGLGLGGKINLYSVFERKHYFYPDLPHGYQITQNSYPIITGGCVRIRNPDK
HHHHHHHHHHCCCCCCEEEHEEHHHCCCCCCCCCCCCEEEECCCCCEEECCEEEECCCCC
VIRINRIHIEQDAGKSIHNLAPGKTYIDLNRAGIPLMEIVSEPDISSPAEAVAYVDKLKL
EEEEEEEEEECCCCCHHHHCCCCCEEEEECCCCCHHHHHHCCCCCCCHHHHHHHHHHHHH
ILQYVCASNANMEKGELRCDANVSVRRGGESGLGTRCEIKNLNSTKSLAQAIEHEAKRQV
HHHHHHHCCCCCCCCCEEECCCCEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
EVLESGGTIVVETKLFDVDTLSTIATRSKESATEYRYFADPDLLPLVLEEKYVEDIKASM
HHHHCCCEEEEEEEEECCHHHHHHHHCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHC
PELPDERENRYVRELNLSPYDANVLVSNVNASRYFDALLELGHQPKLAARWITVELFGLL
CCCCCHHHCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
NKRGIKIVESPVTPALMDELLRLVHSAEISERTAKEVLRKSFEGMGSPKEIVERESLRQV
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TDDKVILGYVKEVLDENPTKLGEYFMGKEKMLAFLVGQVIKKSNGNASPHLVNKVLLEEL
CCCHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ERRRAQNG
HHHHCCCC
>Mature Secondary Structure
MYYIDGKTARWEVVIGLEVHAQILSKNKLFSDAPCDSTKGPNTAVTLFDAAMPGVLPVLN
CEEECCCCEEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHCCCHHHHCC
FNCVEKAIRAGLGLGGKINLYSVFERKHYFYPDLPHGYQITQNSYPIITGGCVRIRNPDK
HHHHHHHHHHCCCCCCEEEHEEHHHCCCCCCCCCCCCEEEECCCCCEEECCEEEECCCCC
VIRINRIHIEQDAGKSIHNLAPGKTYIDLNRAGIPLMEIVSEPDISSPAEAVAYVDKLKL
EEEEEEEEEECCCCCHHHHCCCCCEEEEECCCCCHHHHHHCCCCCCCHHHHHHHHHHHHH
ILQYVCASNANMEKGELRCDANVSVRRGGESGLGTRCEIKNLNSTKSLAQAIEHEAKRQV
HHHHHHHCCCCCCCCCEEECCCCEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
EVLESGGTIVVETKLFDVDTLSTIATRSKESATEYRYFADPDLLPLVLEEKYVEDIKASM
HHHHCCCEEEEEEEEECCHHHHHHHHCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHC
PELPDERENRYVRELNLSPYDANVLVSNVNASRYFDALLELGHQPKLAARWITVELFGLL
CCCCCHHHCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
NKRGIKIVESPVTPALMDELLRLVHSAEISERTAKEVLRKSFEGMGSPKEIVERESLRQV
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TDDKVILGYVKEVLDENPTKLGEYFMGKEKMLAFLVGQVIKKSNGNASPHLVNKVLLEEL
CCCHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ERRRAQNG
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA