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The map label for this gene is gltA

Identifier: 88608282

GI number: 88608282

Start: 651157

End: 652353

Strand: Direct

Name: gltA

Synonym: NSE_0730

Alternate gene names: 88608282

Gene position: 651157-652353 (Clockwise)

Preceding gene: 88608460

Following gene: 88608039

Centisome position: 75.8

GC content: 44.19

Gene sequence:

>1197_bases
ATGAATTCTGAAAAAGTAAAAGTGAATGTGGAGGAATTTTTAAAAAGCACAGCTGGACCCTTCGGTGTCTCTGCCTCTTT
GGAACCAAAGGGATTCTTTTATGATCCAGGGTATGCAAAAACTGCTCCATGCAAATCTAGCATTACTTTCATAGATGGTG
AAAATGGGATACTTCTGCACAGGGGCTACCCAATAGAGGAATTGGCACAAACTTCTGACTTTTTGCAGGTCGGCTACCTT
CTCATTGACGGCAAACTTCCAGATTCCGCTGCTACGCGAGAGTTTGAAGAGAGGATAAAACACGTACCGCAGCTTCCTGA
GTGTGTCTTGAAAAATATCCGTTCTTTCCCAACGAGTGCGCACCCGATGGCAGTTTTAATCTCTGCACTTGCTTCGCTCG
CCTCAGTGTACGGAGAAATGGACTTTAATCAGAAGAAGTTACTTTTAGTTGCCTATACGCCATGGCTCATAGCGCATATA
TACCGACACATCAATTCTCTGGAGTTCGTTGAACCTGACATCAGGCTTCCTTATACCGAGAATTTCCTGTACATGATGTT
TGGTGAGAAAAAGCCCAAAGATGCGGAAATCTTGGACAAAATCTTTATCTTGCATGCGGACCATGGTCAAACTGCTTCGA
CCTCTGTTGCACGGATGGTTGTATCTTCAGGAGCAAGCCCGATTGCGGCATGTTCTGCGGCTACAGCTAGTTTATGGGGA
CCACTGCACGGTGGCGCAAATGAGCGAGTCCTGGAAATGTTGAGAGGTATAGTTGAAAGCGGTGAGAAGGTAGAAAACTT
CATTGAGCGCGTTAAAAGAAAAGAAGAGAGACTTATGGGTTTTGGGCACAGGGTGTATAAAAACTATGATCCTCGTGCAG
CCATATTAAAGCAAAAGGCTCATGATGTGCTAAAAGCCGATTCACCTATTCTAAAAACTGCTGGCGAACTTGAAACAATC
ACAGCAAGGGAAGAGTACTTTCTGAGTCGCAAATTATATCCTAATGTCGACTTCTATTCGGGAATAGTGCTAGACGCACT
GGGAATATCATCTTGCATGTTCACACCGATTTTCGCACTTGCTAGAACAGTAGGTTGGGTTGCACAAATCACAGAGTTCT
TGAGTGACAGTGAACAAAAACTTTGCAGACCTAGACAAATTTACGTAGGCGAACCAAGAAGAAACGTACACGGTTGA

Upstream 100 bases:

>100_bases
GTACCGTGCCTGCATACAAAATTAGAGAAATACCTATAGAATTTTGTTAACCACGCAGCAAGAGCCATTATAGTACTCAA
CTGGAGAATATTTAAAGAAG

Downstream 100 bases:

>100_bases
TCAGCTTTTAGTGGTTCTACGCTCGTGCGGAGAACCACATAAAAGTTACCAAAAGGAACACTTTGTACCACATTTCGGGC
GTTGTGCAGCGATTCTATGC

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNSEKVKVNVEEFLKSTAGPFGVSASLEPKGFFYDPGYAKTAPCKSSITFIDGENGILLHRGYPIEELAQTSDFLQVGYL
LIDGKLPDSAATREFEERIKHVPQLPECVLKNIRSFPTSAHPMAVLISALASLASVYGEMDFNQKKLLLVAYTPWLIAHI
YRHINSLEFVEPDIRLPYTENFLYMMFGEKKPKDAEILDKIFILHADHGQTASTSVARMVVSSGASPIAACSAATASLWG
PLHGGANERVLEMLRGIVESGEKVENFIERVKRKEERLMGFGHRVYKNYDPRAAILKQKAHDVLKADSPILKTAGELETI
TAREEYFLSRKLYPNVDFYSGIVLDALGISSCMFTPIFALARTVGWVAQITEFLSDSEQKLCRPRQIYVGEPRRNVHG

Sequences:

>Translated_398_residues
MNSEKVKVNVEEFLKSTAGPFGVSASLEPKGFFYDPGYAKTAPCKSSITFIDGENGILLHRGYPIEELAQTSDFLQVGYL
LIDGKLPDSAATREFEERIKHVPQLPECVLKNIRSFPTSAHPMAVLISALASLASVYGEMDFNQKKLLLVAYTPWLIAHI
YRHINSLEFVEPDIRLPYTENFLYMMFGEKKPKDAEILDKIFILHADHGQTASTSVARMVVSSGASPIAACSAATASLWG
PLHGGANERVLEMLRGIVESGEKVENFIERVKRKEERLMGFGHRVYKNYDPRAAILKQKAHDVLKADSPILKTAGELETI
TAREEYFLSRKLYPNVDFYSGIVLDALGISSCMFTPIFALARTVGWVAQITEFLSDSEQKLCRPRQIYVGEPRRNVHG
>Mature_398_residues
MNSEKVKVNVEEFLKSTAGPFGVSASLEPKGFFYDPGYAKTAPCKSSITFIDGENGILLHRGYPIEELAQTSDFLQVGYL
LIDGKLPDSAATREFEERIKHVPQLPECVLKNIRSFPTSAHPMAVLISALASLASVYGEMDFNQKKLLLVAYTPWLIAHI
YRHINSLEFVEPDIRLPYTENFLYMMFGEKKPKDAEILDKIFILHADHGQTASTSVARMVVSSGASPIAACSAATASLWG
PLHGGANERVLEMLRGIVESGEKVENFIERVKRKEERLMGFGHRVYKNYDPRAAILKQKAHDVLKADSPILKTAGELETI
TAREEYFLSRKLYPNVDFYSGIVLDALGISSCMFTPIFALARTVGWVAQITEFLSDSEQKLCRPRQIYVGEPRRNVHG

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=319, Percent_Identity=27.2727272727273, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1786939, Length=404, Percent_Identity=45.049504950495, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1786527, Length=366, Percent_Identity=30.8743169398907, Blast_Score=162, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17555174, Length=363, Percent_Identity=28.3746556473829, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6319850, Length=321, Percent_Identity=27.1028037383178, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324328, Length=321, Percent_Identity=26.1682242990654, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24640124, Length=322, Percent_Identity=25.776397515528, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24640126, Length=322, Percent_Identity=25.776397515528, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI21356863, Length=319, Percent_Identity=26.6457680250784, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 44438; Mature: 44438

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSEKVKVNVEEFLKSTAGPFGVSASLEPKGFFYDPGYAKTAPCKSSITFIDGENGILLH
CCCCEEEEEHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEEECCCCEEEE
RGYPIEELAQTSDFLQVGYLLIDGKLPDSAATREFEERIKHVPQLPECVLKNIRSFPTSA
CCCCHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
HPMAVLISALASLASVYGEMDFNQKKLLLVAYTPWLIAHIYRHINSLEFVEPDIRLPYTE
CHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCC
NFLYMMFGEKKPKDAEILDKIFILHADHGQTASTSVARMVVSSGASPIAACSAATASLWG
CEEEEEECCCCCCCHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
PLHGGANERVLEMLRGIVESGEKVENFIERVKRKEERLMGFGHRVYKNYDPRAAILKQKA
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
HDVLKADSPILKTAGELETITAREEYFLSRKLYPNVDFYSGIVLDALGISSCMFTPIFAL
HHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ARTVGWVAQITEFLSDSEQKLCRPRQIYVGEPRRNVHG
HHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MNSEKVKVNVEEFLKSTAGPFGVSASLEPKGFFYDPGYAKTAPCKSSITFIDGENGILLH
CCCCEEEEEHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEEECCCCEEEE
RGYPIEELAQTSDFLQVGYLLIDGKLPDSAATREFEERIKHVPQLPECVLKNIRSFPTSA
CCCCHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
HPMAVLISALASLASVYGEMDFNQKKLLLVAYTPWLIAHIYRHINSLEFVEPDIRLPYTE
CHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCC
NFLYMMFGEKKPKDAEILDKIFILHADHGQTASTSVARMVVSSGASPIAACSAATASLWG
CEEEEEECCCCCCCHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
PLHGGANERVLEMLRGIVESGEKVENFIERVKRKEERLMGFGHRVYKNYDPRAAILKQKA
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
HDVLKADSPILKTAGELETITAREEYFLSRKLYPNVDFYSGIVLDALGISSCMFTPIFAL
HHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ARTVGWVAQITEFLSDSEQKLCRPRQIYVGEPRRNVHG
HHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]