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The map label for this gene is grpE

Identifier: 88608234

GI number: 88608234

Start: 179931

End: 180494

Strand: Direct

Name: grpE

Synonym: NSE_0212

Alternate gene names: 88608234

Gene position: 179931-180494 (Clockwise)

Preceding gene: 88608465

Following gene: 88608758

Centisome position: 20.95

GC content: 41.31

Gene sequence:

>564_bases
GTGAGAGATATGAGTGAAAAGCAGCCAGAAAAACACAAAAAAGCGGAAGGTAAGCAGAATGCTGAATTAAAGGCAGATAT
TCAAGAAAAGCTTCTGAAAGTCGGTTTTGTTTCTGAAGAAGAGTTCAATAGGGAAAGGGAACGGTGGAAAAAGAGGTTGG
CTTATGCACTTGCTGAACAGGAAAATCTTAAAAAAAGTGCTCAAAAGGAAATAGAGAAAGTGCGGGATTTTGCTATTTTG
GACCTTGTTAAAGAGATTTTGGTGAGTGTTGAAAGTCTTGAGAGGGCGGTAGCTCATATGCTTGAACATAATGTTGAAGG
ACCCGTGTTTGAAGGTTCAAAACTTACCCTTGATGCGATTTTTTCCGCTCTTAAAAAAAATGGCATAGAAAAGATCGAAG
CCAAAGGAGCGCGGTTTGATCACGATCTACATCAGGCAGTGAGTACGGTCAAAGCTGCAGATTTGCCTAACAACACGGTT
TTTGAAGTTCTGCAGGATGGCTATACAATTAAGGGGCGCCTTTTGCGTCCGGCAGTGGTGGTTGTTGTGGAAAATTTAGA
GTAA

Upstream 100 bases:

>100_bases
TTAGATCTCGTTCGTGCATGAGAAGTTTTCATTTACTGGATTCGATAATATAACCTATACTGAGTGTGTATGATAATAAG
GTTAACCAACAAAAGTTCGT

Downstream 100 bases:

>100_bases
GACTCTTCTCTTAAAAGGTAAATTGTTTAATGGTACGGTCTACGGTGGAAGATAAAGCTGTTTTGTCTGGGATCCAGCCA
ACTGGGTATGTGCAGCTTGG

Product: co-chaperone GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MRDMSEKQPEKHKKAEGKQNAELKADIQEKLLKVGFVSEEEFNRERERWKKRLAYALAEQENLKKSAQKEIEKVRDFAIL
DLVKEILVSVESLERAVAHMLEHNVEGPVFEGSKLTLDAIFSALKKNGIEKIEAKGARFDHDLHQAVSTVKAADLPNNTV
FEVLQDGYTIKGRLLRPAVVVVVENLE

Sequences:

>Translated_187_residues
MRDMSEKQPEKHKKAEGKQNAELKADIQEKLLKVGFVSEEEFNRERERWKKRLAYALAEQENLKKSAQKEIEKVRDFAIL
DLVKEILVSVESLERAVAHMLEHNVEGPVFEGSKLTLDAIFSALKKNGIEKIEAKGARFDHDLHQAVSTVKAADLPNNTV
FEVLQDGYTIKGRLLRPAVVVVVENLE
>Mature_187_residues
MRDMSEKQPEKHKKAEGKQNAELKADIQEKLLKVGFVSEEEFNRERERWKKRLAYALAEQENLKKSAQKEIEKVRDFAIL
DLVKEILVSVESLERAVAHMLEHNVEGPVFEGSKLTLDAIFSALKKNGIEKIEAKGARFDHDLHQAVSTVKAADLPNNTV
FEVLQDGYTIKGRLLRPAVVVVVENLE

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=151, Percent_Identity=31.1258278145695, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1788967, Length=126, Percent_Identity=34.1269841269841, Blast_Score=80, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324806, Length=150, Percent_Identity=32, Blast_Score=83, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24653432, Length=183, Percent_Identity=26.775956284153, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21274; Mature: 21274

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDMSEKQPEKHKKAEGKQNAELKADIQEKLLKVGFVSEEEFNRERERWKKRLAYALAEQ
CCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ENLKKSAQKEIEKVRDFAILDLVKEILVSVESLERAVAHMLEHNVEGPVFEGSKLTLDAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
FSALKKNGIEKIEAKGARFDHDLHQAVSTVKAADLPNNTVFEVLQDGYTIKGRLLRPAVV
HHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEHHHHHHHHH
VVVENLE
HEECCCC
>Mature Secondary Structure
MRDMSEKQPEKHKKAEGKQNAELKADIQEKLLKVGFVSEEEFNRERERWKKRLAYALAEQ
CCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ENLKKSAQKEIEKVRDFAILDLVKEILVSVESLERAVAHMLEHNVEGPVFEGSKLTLDAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
FSALKKNGIEKIEAKGARFDHDLHQAVSTVKAADLPNNTVFEVLQDGYTIKGRLLRPAVV
HHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEHHHHHHHHH
VVVENLE
HEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA