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The map label for this gene is metG

Identifier: 88608152

GI number: 88608152

Start: 797690

End: 799201

Strand: Direct

Name: metG

Synonym: NSE_0893

Alternate gene names: 88608152

Gene position: 797690-799201 (Clockwise)

Preceding gene: 88608772

Following gene: 88608124

Centisome position: 92.86

GC content: 40.67

Gene sequence:

>1512_bases
ATGCGCTATTACGTTACCACTCCAGTGTATTATGTAAATGATAATCCACATATAGGTCACGCATACAGCAGCTTGATAGC
TGATGTACTGACAAGAGCACTTAGATTAGCCGGTCATGAAGTACGTTTCACAACAGGAACCGATGAGCACGGACAAAAAG
TGCAAAATACAGCCAAAAAACATGGAAAATGTGAAATAGACTTTGTAAACCATGTAAGTAGACGCTTTCGTGATTTATGC
GTGGCAATGCATTACGATTTCTCTGACTTCATACGCACAACCGAAACTAGACATAAAGAAGCTGTTCAAACATTGTGGAG
CACCCTAAGTAAGAATAACTCTATTTCCCTGGGTGAGTACTCAGGATGGTACTCCGAGAGAGACGAAGCCTTTTATACAG
AAGCCGAACTGCAGAACAGCCTTGCTCCAACAGGGTCACCCGTGTCGTGGACAAAAGAAGCATGTTACTTTTTTAAACTC
TCTCAATTCTCTGAACCGCTAAAGAAGCTTTATGCTGAGAATCCAACATTTATACAACCAAAAAGTAGATTCAATGAGAT
GCTGGCCTTCCTTAATACTGGTATGCACGACCTATCCATTTCTAGAACTCGAGTCCAGTGGGGAATAAATGTTCCAGATA
ACCCAACACACACAGTGTATGTCTGGCTTGATGCACTAACGAATTATATATCTTCCGTTGGCTACCCTAATACAAGTGCT
GACTCTTTATTTTCCGCTTTTTGGGACAATGCACGCATAATACACATAATAGGAAAGGACATATTACGCTTCCACTCAAT
ATACTGGCCAGCCTTCCTTACGGCAGCAAATTTGAAAACGCCCGATGTGATACTTGCACACGGCTGGTGGCTTAATGATG
GCGAAAAAATTTCGAAATCACTTGGAAACATAATAGACCCATTCGAATTAATTTCTGAATTTGGTGTAGATCCAGTACGA
TATTTCTTTATAAGAGAAATAACAGTTGGGAAAGATGGTAACTTCAGCAAGGTAAATTTAAAAGAACGAATCAATAGTGA
ACTAGCTGACAATATAGGAAACCTTTTACATAGGACACTCACTTTAACAGCTAAGTTACTCGGCCCAAAAGTGAGTGAGA
TCTACGAATCAGAAAATGAGCTCCTAGAATTTGCGTACTCGCTAGCTACAGAGTACAAAAATGACATCGAACTACTCGAC
CTTAATAGCAGTTTGGACAGGATAATACAACTTTCCAATAAGGCAAACGAATATATATCCATGGAAGAGCCCTGGAAACT
AAAGGAAAAACCTGAAACAGCAGGTAGAATCTTACATTCAGTACTGGAAGCACTCAGAATAATTGCTATAGGACTTCAGC
CATTTGTTCCACAATCTGCAAGTACTATGCTCGATCTGCTTGCAGTTCCACCAGAAGAACGAAAACTAGCAAACATGAAC
AAGGAATCACGTATAAGACCCGGCACACAGATCAAGCTGCCGGCCTCACCACTGTTCAGAAAACACCAATAG

Upstream 100 bases:

>100_bases
TTTTCACATAACCCTTGCGCCTTACTAAGTCTCTAATACGCAACCTATGAAAGGAGGCAACAAATATGTTAGAGTGGCCT
CAATTCTTTTCGCTCATCCC

Downstream 100 bases:

>100_bases
TTGTTGTAACTCCACAAATACAGAGGGAAACAAGACTAAGAGAACGTGGCAACCCATTCTTTGAGTTGCGACAACGAAAG
AGACCCAACTTTACGATCCA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MRYYVTTPVYYVNDNPHIGHAYSSLIADVLTRALRLAGHEVRFTTGTDEHGQKVQNTAKKHGKCEIDFVNHVSRRFRDLC
VAMHYDFSDFIRTTETRHKEAVQTLWSTLSKNNSISLGEYSGWYSERDEAFYTEAELQNSLAPTGSPVSWTKEACYFFKL
SQFSEPLKKLYAENPTFIQPKSRFNEMLAFLNTGMHDLSISRTRVQWGINVPDNPTHTVYVWLDALTNYISSVGYPNTSA
DSLFSAFWDNARIIHIIGKDILRFHSIYWPAFLTAANLKTPDVILAHGWWLNDGEKISKSLGNIIDPFELISEFGVDPVR
YFFIREITVGKDGNFSKVNLKERINSELADNIGNLLHRTLTLTAKLLGPKVSEIYESENELLEFAYSLATEYKNDIELLD
LNSSLDRIIQLSNKANEYISMEEPWKLKEKPETAGRILHSVLEALRIIAIGLQPFVPQSASTMLDLLAVPPEERKLANMN
KESRIRPGTQIKLPASPLFRKHQ

Sequences:

>Translated_503_residues
MRYYVTTPVYYVNDNPHIGHAYSSLIADVLTRALRLAGHEVRFTTGTDEHGQKVQNTAKKHGKCEIDFVNHVSRRFRDLC
VAMHYDFSDFIRTTETRHKEAVQTLWSTLSKNNSISLGEYSGWYSERDEAFYTEAELQNSLAPTGSPVSWTKEACYFFKL
SQFSEPLKKLYAENPTFIQPKSRFNEMLAFLNTGMHDLSISRTRVQWGINVPDNPTHTVYVWLDALTNYISSVGYPNTSA
DSLFSAFWDNARIIHIIGKDILRFHSIYWPAFLTAANLKTPDVILAHGWWLNDGEKISKSLGNIIDPFELISEFGVDPVR
YFFIREITVGKDGNFSKVNLKERINSELADNIGNLLHRTLTLTAKLLGPKVSEIYESENELLEFAYSLATEYKNDIELLD
LNSSLDRIIQLSNKANEYISMEEPWKLKEKPETAGRILHSVLEALRIIAIGLQPFVPQSASTMLDLLAVPPEERKLANMN
KESRIRPGTQIKLPASPLFRKHQ
>Mature_503_residues
MRYYVTTPVYYVNDNPHIGHAYSSLIADVLTRALRLAGHEVRFTTGTDEHGQKVQNTAKKHGKCEIDFVNHVSRRFRDLC
VAMHYDFSDFIRTTETRHKEAVQTLWSTLSKNNSISLGEYSGWYSERDEAFYTEAELQNSLAPTGSPVSWTKEACYFFKL
SQFSEPLKKLYAENPTFIQPKSRFNEMLAFLNTGMHDLSISRTRVQWGINVPDNPTHTVYVWLDALTNYISSVGYPNTSA
DSLFSAFWDNARIIHIIGKDILRFHSIYWPAFLTAANLKTPDVILAHGWWLNDGEKISKSLGNIIDPFELISEFGVDPVR
YFFIREITVGKDGNFSKVNLKERINSELADNIGNLLHRTLTLTAKLLGPKVSEIYESENELLEFAYSLATEYKNDIELLD
LNSSLDRIIQLSNKANEYISMEEPWKLKEKPETAGRILHSVLEALRIIAIGLQPFVPQSASTMLDLLAVPPEERKLANMN
KESRIRPGTQIKLPASPLFRKHQ

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=519, Percent_Identity=38.3429672447013, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI14043022, Length=554, Percent_Identity=22.7436823104693, Blast_Score=116, Evalue=5e-26,
Organism=Escherichia coli, GI1788432, Length=502, Percent_Identity=23.5059760956175, Blast_Score=159, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI212645096, Length=502, Percent_Identity=35.8565737051793, Blast_Score=291, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI71992781, Length=395, Percent_Identity=34.4303797468354, Blast_Score=204, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17540794, Length=546, Percent_Identity=23.992673992674, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321610, Length=523, Percent_Identity=34.225621414914, Blast_Score=282, Evalue=8e-77,
Organism=Drosophila melanogaster, GI21358483, Length=503, Percent_Identity=39.9602385685885, Blast_Score=348, Evalue=4e-96,
Organism=Drosophila melanogaster, GI24655311, Length=560, Percent_Identity=23.9285714285714, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 57478; Mature: 57478

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYYVTTPVYYVNDNPHIGHAYSSLIADVLTRALRLAGHEVRFTTGTDEHGQKVQNTAKK
CCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHH
HGKCEIDFVNHVSRRFRDLCVAMHYDFSDFIRTTETRHKEAVQTLWSTLSKNNSISLGEY
CCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCC
SGWYSERDEAFYTEAELQNSLAPTGSPVSWTKEACYFFKLSQFSEPLKKLYAENPTFIQP
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECC
KSRFNEMLAFLNTGMHDLSISRTRVQWGINVPDNPTHTVYVWLDALTNYISSVGYPNTSA
HHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCH
DSLFSAFWDNARIIHIIGKDILRFHSIYWPAFLTAANLKTPDVILAHGWWLNDGEKISKS
HHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECEECCCHHHHHHH
LGNIIDPFELISEFGVDPVRYFFIREITVGKDGNFSKVNLKERINSELADNIGNLLHRTL
HCCCCCHHHHHHHHCCCHHHHHEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
TLTAKLLGPKVSEIYESENELLEFAYSLATEYKNDIELLDLNSSLDRIIQLSNKANEYIS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCHHHCCC
MEEPWKLKEKPETAGRILHSVLEALRIIAIGLQPFVPQSASTMLDLLAVPPEERKLANMN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHCCC
KESRIRPGTQIKLPASPLFRKHQ
HHHCCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MRYYVTTPVYYVNDNPHIGHAYSSLIADVLTRALRLAGHEVRFTTGTDEHGQKVQNTAKK
CCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHH
HGKCEIDFVNHVSRRFRDLCVAMHYDFSDFIRTTETRHKEAVQTLWSTLSKNNSISLGEY
CCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCC
SGWYSERDEAFYTEAELQNSLAPTGSPVSWTKEACYFFKLSQFSEPLKKLYAENPTFIQP
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECC
KSRFNEMLAFLNTGMHDLSISRTRVQWGINVPDNPTHTVYVWLDALTNYISSVGYPNTSA
HHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCH
DSLFSAFWDNARIIHIIGKDILRFHSIYWPAFLTAANLKTPDVILAHGWWLNDGEKISKS
HHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECEECCCHHHHHHH
LGNIIDPFELISEFGVDPVRYFFIREITVGKDGNFSKVNLKERINSELADNIGNLLHRTL
HCCCCCHHHHHHHHCCCHHHHHEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
TLTAKLLGPKVSEIYESENELLEFAYSLATEYKNDIELLDLNSSLDRIIQLSNKANEYIS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCHHHCCC
MEEPWKLKEKPETAGRILHSVLEALRIIAIGLQPFVPQSASTMLDLLAVPPEERKLANMN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHCCC
KESRIRPGTQIKLPASPLFRKHQ
HHHCCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA