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The map label for this gene is thrS

Identifier: 88608148

GI number: 88608148

Start: 238350

End: 240263

Strand: Reverse

Name: thrS

Synonym: NSE_0294

Alternate gene names: 88608148

Gene position: 240263-238350 (Counterclockwise)

Preceding gene: 88608295

Following gene: 88608274

Centisome position: 27.97

GC content: 41.8

Gene sequence:

>1914_bases
TTGGGACGTGTCGTGTTAACCGTAACTCTTTTGTCATCTTCTTTCAGCCTATCCTCTGGTAAGACCGTTGGATCTGTAAT
ATCTGATTTTGATGCTGAGATTTTTTCTAAGACAATTGCAGCTTCGGTAAATGGGGTTAGTGTTGATCTCTCTTACCGTC
TCGACTGTGATGCAAATATAGAACCCATTCTCCTCGAAAGCGAAGCGGGAATTGAGATACTAAGACATGATTGTGCTCAT
GTCATGGCACAAGCAGTACAGGAACTATACCCAGAAACGAAATTAGGAATAGGACCAGTCATTGATGATGGTTTTTATTA
TGACTTTTCTACTCCCATCGCTTTCTCCGACAGTGATTTCGAAAAAATAGAAGAGAAGATTAGAGAAATCATTCAGAGGA
ACTACCGGATAATACGTGAAGAATGGAGCAGAGACGATGCAGTTTCGTTTTTCCAAAAAAAGGGCGAAAAGTATAAGGTT
GAACTTATCAGTAGGATTCCGCCTGAAGAAAAGATTTCTATATACAAGCAGGGTGATTTTATTGACTTGTGCCGTGGTCC
GCATGCCCCTTCGACAGGTTGGTTGAAACATTTTAAATTACTGAAGGTATCGACTGCTTACTGGCTTGGTGACGCAAAAA
GCGACTCTTTGCAGCGTATATATGGCACCGCATGGGCAACAAAAGACGGATTGGAAGCTTATCTTTCCTTCCTACATGAA
GCAAAAAAGAGGGATCATCGTGCTATTGGCAAGACAATGGAACTTTTTCATCTACAAGAAGAAGCTCAAGGACAGGTCTT
TTGGCACGAGAATGGCTTCATCCTTTACAGAGTATTGGAGAATTACATCCGTAACAAGCTCAAGACACACGGTTATCGCG
AGGTAAAAACTCCACTCGTATACAACAAATGCCTGTGGGAAAGATCAGGACATTGGTCTAAGTTCAAAGAGAATATGTTC
GTTATAAGATCGGATGATGGTGAATTTTCACTAAAACCGATGAACTGTCCTGCACATATTGAGGTCTACAAGCAGTCTCT
AAAAAGTTATAGAGATTTGCCACTGCGGCTAGCAGAATTTGGTATGTGTCACAGAAATGAAGCTTCTGGAGCACTGCATG
GTCTAATGAGGGTCAGAGGTTTTGTTCAGGATGATGCACATATTTTCTGTACACCTCAGCAAATCTGTGACGAGACTATT
GCGTTTTGCGGATTACTAAAAGAGGTCTACAGGGATCTTGGATTCACAGAAGTAGAGGTGCGCTTTTCAGATCGCCCAGA
AGTACGTCTTGGAAATGACGAAACCTGGGATAAGGCAGAGATTGCTCTGAAATCTGCGATGGATAAAGCAGGGTTGATTT
ATACACTCAACCCCGGAGAAGGAGCGTTCTATGGACCAAAAATAGAGTTTGTACTGGTAGATGCACTAAAAAGAAAATGG
CAATGTGGAACTTTGCAGGTAGATTTTGTGTTGCCTGAACGTCTGGATGCAACCTATATAGACCATGAAGGGAAAAAGCA
GCACCCTGTAATGCTGCACAGAGCTATTTTAGGTACGTTTGAACGTTTCTTGGGTATCCTGATTGAACATTATGATGGCA
AATTTCCATTGTGGCTCGCTCCCACACAGGTAGCAGTCCTCACCATTACGGAAAAGGTTGAAGAATATGCAAAGTCGCTA
CTGAACCATCTATCCAAAACAGGTATACGTGCTGTCTCTGACTTTAGCAATCAAAGAATTGATTACAAAATCAGATATCA
TACTACAAGAAGGGTCCCAATTTTGTGGATCATCGGTAGGATTGAGGAAAAAAGCAATAGCGTCACGGTACGAAGAATGG
GTTCAAGTGAATCTGAAGTGAAGACAGTTGATACAGCCCTTGGGGATATATTGAGGGAGTTCAATGTTAGATAA

Upstream 100 bases:

>100_bases
GTGCGTTCGAATCGCACCGAGATCACGGGTTTTTAGAGTTTCTTAGGAGCATTTTTTTGGATCCTGTGTTCGCGGTGCTA
CTTTTGGTTGAGAGTCACAT

Downstream 100 bases:

>100_bases
ATAAGTAGTACTAATATAAGAAGAATTTGAGGTTCAATAAATTTGAAGAATAAAGAAAAAAGTTTTACTAACCGTACTCC
AAGTAGGTCTAATACAGACA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 637; Mature: 636

Protein sequence:

>637_residues
MGRVVLTVTLLSSSFSLSSGKTVGSVISDFDAEIFSKTIAASVNGVSVDLSYRLDCDANIEPILLESEAGIEILRHDCAH
VMAQAVQELYPETKLGIGPVIDDGFYYDFSTPIAFSDSDFEKIEEKIREIIQRNYRIIREEWSRDDAVSFFQKKGEKYKV
ELISRIPPEEKISIYKQGDFIDLCRGPHAPSTGWLKHFKLLKVSTAYWLGDAKSDSLQRIYGTAWATKDGLEAYLSFLHE
AKKRDHRAIGKTMELFHLQEEAQGQVFWHENGFILYRVLENYIRNKLKTHGYREVKTPLVYNKCLWERSGHWSKFKENMF
VIRSDDGEFSLKPMNCPAHIEVYKQSLKSYRDLPLRLAEFGMCHRNEASGALHGLMRVRGFVQDDAHIFCTPQQICDETI
AFCGLLKEVYRDLGFTEVEVRFSDRPEVRLGNDETWDKAEIALKSAMDKAGLIYTLNPGEGAFYGPKIEFVLVDALKRKW
QCGTLQVDFVLPERLDATYIDHEGKKQHPVMLHRAILGTFERFLGILIEHYDGKFPLWLAPTQVAVLTITEKVEEYAKSL
LNHLSKTGIRAVSDFSNQRIDYKIRYHTTRRVPILWIIGRIEEKSNSVTVRRMGSSESEVKTVDTALGDILREFNVR

Sequences:

>Translated_637_residues
MGRVVLTVTLLSSSFSLSSGKTVGSVISDFDAEIFSKTIAASVNGVSVDLSYRLDCDANIEPILLESEAGIEILRHDCAH
VMAQAVQELYPETKLGIGPVIDDGFYYDFSTPIAFSDSDFEKIEEKIREIIQRNYRIIREEWSRDDAVSFFQKKGEKYKV
ELISRIPPEEKISIYKQGDFIDLCRGPHAPSTGWLKHFKLLKVSTAYWLGDAKSDSLQRIYGTAWATKDGLEAYLSFLHE
AKKRDHRAIGKTMELFHLQEEAQGQVFWHENGFILYRVLENYIRNKLKTHGYREVKTPLVYNKCLWERSGHWSKFKENMF
VIRSDDGEFSLKPMNCPAHIEVYKQSLKSYRDLPLRLAEFGMCHRNEASGALHGLMRVRGFVQDDAHIFCTPQQICDETI
AFCGLLKEVYRDLGFTEVEVRFSDRPEVRLGNDETWDKAEIALKSAMDKAGLIYTLNPGEGAFYGPKIEFVLVDALKRKW
QCGTLQVDFVLPERLDATYIDHEGKKQHPVMLHRAILGTFERFLGILIEHYDGKFPLWLAPTQVAVLTITEKVEEYAKSL
LNHLSKTGIRAVSDFSNQRIDYKIRYHTTRRVPILWIIGRIEEKSNSVTVRRMGSSESEVKTVDTALGDILREFNVR
>Mature_636_residues
GRVVLTVTLLSSSFSLSSGKTVGSVISDFDAEIFSKTIAASVNGVSVDLSYRLDCDANIEPILLESEAGIEILRHDCAHV
MAQAVQELYPETKLGIGPVIDDGFYYDFSTPIAFSDSDFEKIEEKIREIIQRNYRIIREEWSRDDAVSFFQKKGEKYKVE
LISRIPPEEKISIYKQGDFIDLCRGPHAPSTGWLKHFKLLKVSTAYWLGDAKSDSLQRIYGTAWATKDGLEAYLSFLHEA
KKRDHRAIGKTMELFHLQEEAQGQVFWHENGFILYRVLENYIRNKLKTHGYREVKTPLVYNKCLWERSGHWSKFKENMFV
IRSDDGEFSLKPMNCPAHIEVYKQSLKSYRDLPLRLAEFGMCHRNEASGALHGLMRVRGFVQDDAHIFCTPQQICDETIA
FCGLLKEVYRDLGFTEVEVRFSDRPEVRLGNDETWDKAEIALKSAMDKAGLIYTLNPGEGAFYGPKIEFVLVDALKRKWQ
CGTLQVDFVLPERLDATYIDHEGKKQHPVMLHRAILGTFERFLGILIEHYDGKFPLWLAPTQVAVLTITEKVEEYAKSLL
NHLSKTGIRAVSDFSNQRIDYKIRYHTTRRVPILWIIGRIEEKSNSVTVRRMGSSESEVKTVDTALGDILREFNVR

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=605, Percent_Identity=38.5123966942149, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI38570111, Length=593, Percent_Identity=38.2799325463744, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI20070344, Length=632, Percent_Identity=36.5506329113924, Blast_Score=380, Evalue=1e-105,
Organism=Escherichia coli, GI1788013, Length=587, Percent_Identity=48.8926746166951, Blast_Score=629, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=140, Percent_Identity=30.7142857142857, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71984192, Length=588, Percent_Identity=39.9659863945578, Blast_Score=448, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI71984184, Length=588, Percent_Identity=39.9659863945578, Blast_Score=448, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322112, Length=655, Percent_Identity=38.3206106870229, Blast_Score=459, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322654, Length=448, Percent_Identity=33.2589285714286, Blast_Score=271, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24583839, Length=638, Percent_Identity=36.833855799373, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24583842, Length=638, Percent_Identity=36.833855799373, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI28574141, Length=638, Percent_Identity=36.833855799373, Blast_Score=446, Evalue=1e-125,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_NEOSM (Q2GEB1)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506185.1
- ProteinModelPortal:   Q2GEB1
- SMR:   Q2GEB1
- STRING:   Q2GEB1
- GeneID:   3931448
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0294
- TIGR:   NSE_0294
- eggNOG:   COG0441
- HOGENOM:   HBG352811
- OMA:   TAKDPEC
- PhylomeDB:   Q2GEB1
- ProtClustDB:   PRK00413
- BioCyc:   NSEN222891:NSE_0294-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 73073; Mature: 72942

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRVVLTVTLLSSSFSLSSGKTVGSVISDFDAEIFSKTIAASVNGVSVDLSYRLDCDANI
CCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCC
EPILLESEAGIEILRHDCAHVMAQAVQELYPETKLGIGPVIDDGFYYDFSTPIAFSDSDF
CEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCEEEECCCCEEECCCHH
EKIEEKIREIIQRNYRIIREEWSRDDAVSFFQKKGEKYKVELISRIPPEEKISIYKQGDF
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEHHHHHCCCHHHEEEEECCCE
IDLCRGPHAPSTGWLKHFKLLKVSTAYWLGDAKSDSLQRIYGTAWATKDGLEAYLSFLHE
EEECCCCCCCCCHHHHHHHHHEEEHHEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
AKKRDHRAIGKTMELFHLQEEAQGQVFWHENGFILYRVLENYIRNKLKTHGYREVKTPLV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHHHCCCHHHCCCHH
YNKCLWERSGHWSKFKENMFVIRSDDGEFSLKPMNCPAHIEVYKQSLKSYRDLPLRLAEF
HHHHHHCCCCCHHHHCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHH
GMCHRNEASGALHGLMRVRGFVQDDAHIFCTPQQICDETIAFCGLLKEVYRDLGFTEVEV
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
RFSDRPEVRLGNDETWDKAEIALKSAMDKAGLIYTLNPGEGAFYGPKIEFVLVDALKRKW
EECCCCCEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEECCEEEEEEEHHHHCCC
QCGTLQVDFVLPERLDATYIDHEGKKQHPVMLHRAILGTFERFLGILIEHYDGKFPLWLA
CCCCEEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PTQVAVLTITEKVEEYAKSLLNHLSKTGIRAVSDFSNQRIDYKIRYHTTRRVPILWIIGR
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEE
IEEKSNSVTVRRMGSSESEVKTVDTALGDILREFNVR
EECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GRVVLTVTLLSSSFSLSSGKTVGSVISDFDAEIFSKTIAASVNGVSVDLSYRLDCDANI
CEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCC
EPILLESEAGIEILRHDCAHVMAQAVQELYPETKLGIGPVIDDGFYYDFSTPIAFSDSDF
CEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCEEEECCCCEEECCCHH
EKIEEKIREIIQRNYRIIREEWSRDDAVSFFQKKGEKYKVELISRIPPEEKISIYKQGDF
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEHHHHHCCCHHHEEEEECCCE
IDLCRGPHAPSTGWLKHFKLLKVSTAYWLGDAKSDSLQRIYGTAWATKDGLEAYLSFLHE
EEECCCCCCCCCHHHHHHHHHEEEHHEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
AKKRDHRAIGKTMELFHLQEEAQGQVFWHENGFILYRVLENYIRNKLKTHGYREVKTPLV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHHHCCCHHHCCCHH
YNKCLWERSGHWSKFKENMFVIRSDDGEFSLKPMNCPAHIEVYKQSLKSYRDLPLRLAEF
HHHHHHCCCCCHHHHCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHH
GMCHRNEASGALHGLMRVRGFVQDDAHIFCTPQQICDETIAFCGLLKEVYRDLGFTEVEV
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
RFSDRPEVRLGNDETWDKAEIALKSAMDKAGLIYTLNPGEGAFYGPKIEFVLVDALKRKW
EECCCCCEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEECCEEEEEEEHHHHCCC
QCGTLQVDFVLPERLDATYIDHEGKKQHPVMLHRAILGTFERFLGILIEHYDGKFPLWLA
CCCCEEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PTQVAVLTITEKVEEYAKSLLNHLSKTGIRAVSDFSNQRIDYKIRYHTTRRVPILWIIGR
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEE
IEEKSNSVTVRRMGSSESEVKTVDTALGDILREFNVR
EECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA