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The map label for this gene is folK

Identifier: 88608128

GI number: 88608128

Start: 402270

End: 402830

Strand: Reverse

Name: folK

Synonym: NSE_0486

Alternate gene names: 88608128

Gene position: 402830-402270 (Counterclockwise)

Preceding gene: 88608194

Following gene: 88608700

Centisome position: 46.89

GC content: 38.15

Gene sequence:

>561_bases
GTGATGCGTCAGGGGAATTTAGTAGTACTTGGCCTGGGTTCAAACGTAGGTGATACGAAAAAAAACATTTTAGCTGCAGT
AAGAAAGTTACCTGTGACAAGAAAAATACTTTCCCCAATCATTAAAACGAAAGCGCTTCTAACAAAGAATGCTCCTGAAG
AGTGGAATATGGATTATCATAATGCTGTTGTTATCGGAAGAGCACTCTTCGAGCCATTCGAGACACTGAACCGCCTCAAA
AAGATAGAAAAGCAGATGAAAAAGATCAAATTAGGTAAGTATTCTCCAAGAACTATAGATATCGACATCTTATTTTGGGG
GAAGGCTATCCTATTTGAAAAACAACTCAGGATCCCGCACAAAGAAGTTCTGAACCGCTACTTTACGCTCAAACCAGCAG
TAGAAATAGTTCCACTTCTGCATCATCCAATCTGTAAAATAAACATGAGGCAGGCTTTACTTAACCACAATAGAAACACT
AGAAGAAGTTTTACCTCTGAGAATCACGTTAGTCCACAAAGTAAATACGCAAAGCCTTTGTATCAACTAAAAGAATATTA
G

Upstream 100 bases:

>100_bases
AAATACCTGAACCCAAAATGCATAAAATATAGGAAAATCTTTTATATTTCAGGGAGGCATTATACAATACCGCCCTGTGC
TTTTCCATCATGATCCTCTG

Downstream 100 bases:

>100_bases
CTCCAGTATATATAGGTATATATACCATTACCATATCTATAAGGTCTCTGGTTTTACTATTACCTTGCCAATTGATTCCA
CTTATCCACAAATCTCATCT

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase; 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK; Dihydropteroate synthase; DHPS; Dihydropteroate pyrophosphorylase [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MMRQGNLVVLGLGSNVGDTKKNILAAVRKLPVTRKILSPIIKTKALLTKNAPEEWNMDYHNAVVIGRALFEPFETLNRLK
KIEKQMKKIKLGKYSPRTIDIDILFWGKAILFEKQLRIPHKEVLNRYFTLKPAVEIVPLLHHPICKINMRQALLNHNRNT
RRSFTSENHVSPQSKYAKPLYQLKEY

Sequences:

>Translated_186_residues
MMRQGNLVVLGLGSNVGDTKKNILAAVRKLPVTRKILSPIIKTKALLTKNAPEEWNMDYHNAVVIGRALFEPFETLNRLK
KIEKQMKKIKLGKYSPRTIDIDILFWGKAILFEKQLRIPHKEVLNRYFTLKPAVEIVPLLHHPICKINMRQALLNHNRNT
RRSFTSENHVSPQSKYAKPLYQLKEY
>Mature_186_residues
MMRQGNLVVLGLGSNVGDTKKNILAAVRKLPVTRKILSPIIKTKALLTKNAPEEWNMDYHNAVVIGRALFEPFETLNRLK
KIEKQMKKIKLGKYSPRTIDIDILFWGKAILFEKQLRIPHKEVLNRYFTLKPAVEIVPLLHHPICKINMRQALLNHNRNT
RRSFTSENHVSPQSKYAKPLYQLKEY

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=154, Percent_Identity=31.8181818181818, Blast_Score=69, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324073, Length=146, Percent_Identity=32.1917808219178, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006390
- InterPro:   IPR011005
- InterPro:   IPR000550
- InterPro:   IPR000489 [H]

Pfam domain/function: PF01288 HPPK; PF00809 Pterin_bind [H]

EC number: =2.7.6.3; =2.5.1.15 [H]

Molecular weight: Translated: 21621; Mature: 21621

Theoretical pI: Translated: 10.92; Mature: 10.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRQGNLVVLGLGSNVGDTKKNILAAVRKLPVTRKILSPIIKTKALLTKNAPEEWNMDYH
CCCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCH
NAVVIGRALFEPFETLNRLKKIEKQMKKIKLGKYSPRTIDIDILFWGKAILFEKQLRIPH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHHCCCH
KEVLNRYFTLKPAVEIVPLLHHPICKINMRQALLNHNRNTRRSFTSENHVSPQSKYAKPL
HHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHCCCCCCCCCCHHHHHHHH
YQLKEY
HHHHCC
>Mature Secondary Structure
MMRQGNLVVLGLGSNVGDTKKNILAAVRKLPVTRKILSPIIKTKALLTKNAPEEWNMDYH
CCCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCH
NAVVIGRALFEPFETLNRLKKIEKQMKKIKLGKYSPRTIDIDILFWGKAILFEKQLRIPH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHHCCCH
KEVLNRYFTLKPAVEIVPLLHHPICKINMRQALLNHNRNTRRSFTSENHVSPQSKYAKPL
HHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHCCCCCCCCCCHHHHHHHH
YQLKEY
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9784136 [H]