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The map label for this gene is clpB

Identifier: 88608018

GI number: 88608018

Start: 94667

End: 97231

Strand: Reverse

Name: clpB

Synonym: NSE_0119

Alternate gene names: 88608018

Gene position: 97231-94667 (Counterclockwise)

Preceding gene: 88608745

Following gene: 88608164

Centisome position: 11.32

GC content: 42.88

Gene sequence:

>2565_bases
ATGCGGTTCGACAAATTTACAGATAATTCAGCGTCAATCATTCAAAGCGCGCAGCTAGATGCGGTAAGCATGGGGCATCA
GACGCTTACATCGCTTCACATACTTAGGGCGCTGCTGAAGAATACCAATGGTATTGTCACCCAACTACTAGAACAATGCG
GCGCAAATTTAGACGTGATTCTAAATAGCGTAGAACTGACACTGAGAAAGCTACCATCCGTGAGTGGCCAAGGTGCAGGA
CACATTCATCTAACGCCGGATGCAGCACAGACAATACAGACATCAATTGAAATAGCTGGAAGGTCACAAGACAGTTTCGT
CACTGTAGAAAGACTCTTGCAAGGTGTAGCAGCAACAGAATCTGAAGCATCTAAAATTCTAAAGGAACAAGGTGTAGACG
CAGCAAAGTTGCAACAAATCATTGACGCAAATAGGGGAAGTAGGAGAGCTGATTCTGCAAATGCGGAGAACAACTTCAAC
GCTCTTAGACGCTATACAAAAGACCTAACTGAGATTGCACGCTCGGGAAAACTTGACCCGGTTATCGGCAGAGACGAAGA
AATCCGGAGGGTGATGCAGGTCTTGTCTAGACGAACAAAAAATAATCCGGTACTCATTGGTGATCCAGGCGTTGGCAAGA
CGGCAGTAGTTGAAGGTCTTGCAATGCGGATGGTCGCAAAAGATGTTCCACAAAATTTGGCAAGCGCTTCTCTACTTTCA
CTTGACTTAGGTGCACTAATAGCAGGTTCAAAGTACAGAGGTGAATTCGAGGAAAGATTAAAATCAGTAATCAACGAACT
TGCAGATTCTGGGGGTAGCGTCATTCTTTTTATAGATGAGCTCCACACACTCGTAGGTGCGGGTGCAACAGAAGGTGCAA
TGGACGCCTCGAATTTACTAAAACCAGCACTTGCACGCGGGGAAATACACTGCGTAGGTGCAACTACACTGGAAGAGTAC
AAGAAACACATAGAAAAGGATGCCGCACTAGCGAGGAGATTTCAGCCGATTTTCGTTGGACAACCCACAGAAGAGGATAC
CATTTCAATACTGCGCGGCATAAAAGAGAAGTATGAAGTCCACCACGGAATAAGAATTGCTGATAGCGCAGTTGTCTCCG
CAGCAGTTCTCTCAAATCGGTATATCACAGACAGATTCCTCCCAGATAAGGCCATAGACTTAATGGACGAGGCTGCAAGT
AAGATCAGGATTGAATCTGACAGTAAGCCTGAGGTAATAGATGAACTCGACAGGAAAATCATACAACTAAAAATAGAAGC
AGAAGCACTGAAAAAAGAAAAGGATGCTAATTCTATCGGCAGATTGGAAAAAATAAAGCAGGATCTAGAAGGTCTTGAAA
AAAAACAAGCGGATCTCGAAAGCTCCTGGCAAGCTGAAAAGTCAAAGCTTAAAGAGATACAAAAATTGAAAGAGGAACTC
AACGAAGCACGGTATTCACTGGAGATTGCACAGAGAAATGGTGATCTTGCAACCGCAGGAAAGATCCTATACGGCACCAT
TCCAGCCTTGGAAAATAGATTGGTCAATGAAGAAAAACAAGTAGGCGGTACACTCCTAAAAAGAGAGGTCACAGAGGACG
ACATAGCAAACATTGTTGCCAAATGGACAGGAATTCCTGTTGAAAAAATGATGGGGAGTGAACAACAAAGACTTTTAAAG
ATAGAAGATGAATTGAAAAAACACGTCATAGGACAAGATGAAGCCGTAGAAGCAGTCAGCAGTGCGGTAAAACGCTCTAG
AGCTGGCGTCCAGGATGCAAATCGTCCGCTTGGATCTTTCATGTTTTTAGGTTCAACAGGCGTTGGAAAAACAGAGCTCA
GCAAAGCACTAGCAAAGTTCCTGTTCGATAATGAAACAGCACTGCTTCGCATTGATATGTCCGAATATATGGAAAAACAT
GCCGTAGCACGATTGATAGGTGCACCTCCTGGATATGTCGGGTATGAGGAAGGTGGAATACTTACTGAAGCAGTAAGACG
GCGTCCATATCAGGTAATCTTATTCGACGAAGTGGAAAAAGCACATCATGATGTGTTCAATATATTGCTTCAAGTATTAG
ACGAGGGCAGACTAACCGATACTCAAGGCCATGTCGTTGATTTTAAAAATACAATATTAATTCTTACATCAAACCTGGGA
TCGGATGTTTTATCGGAAGTTACACAAATCACCGAGGTTGAGAAAACTAAAATAATGGAAATAGTCAGGCAATTCTTCCG
TCCTGAGTTTTTGAACCGTCTTGACGAGATCCTTTTCTTCAATCGCTTGAGCAAAGAAAACATAGAAAAGATACTGGAGA
TTCAATTGCATAAACTACAGGAACGCATAAAAACCAAGAACCTTCACCTTTCTTTTACTCACGAAGCCAAGAGGTGGATA
TGTGAACAGGGGTATCATCCAGTATACGGTGCTCGACCATTAAAACGCGTGATTCAGCAATACGTTTATAATCCACTAGC
ACAAGCAATCCTCTCGGGTAGCCTGCCTGAAAATGGCAAAATTATATTCGATAAAATTGGTAACAAGCTAGAAATCCAGT
ATTAG

Upstream 100 bases:

>100_bases
CACATTACCGTAGTTGCTTAGTCTACACACAACTCTCATAATTCACTGATGAAAGTATCCAATTTATGCTTACATATACT
ATGTGCTATCTACTATAAAC

Downstream 100 bases:

>100_bases
CAAGAACTGGATATGAACATTTGGTGCGTTGGGAAGGATAGGATTGCAGGCGTGTGTCTTGTATGGACACCGCAATCCTA
TCTACATGTTAACAACTCAG

Product: ClpB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 854; Mature: 854

Protein sequence:

>854_residues
MRFDKFTDNSASIIQSAQLDAVSMGHQTLTSLHILRALLKNTNGIVTQLLEQCGANLDVILNSVELTLRKLPSVSGQGAG
HIHLTPDAAQTIQTSIEIAGRSQDSFVTVERLLQGVAATESEASKILKEQGVDAAKLQQIIDANRGSRRADSANAENNFN
ALRRYTKDLTEIARSGKLDPVIGRDEEIRRVMQVLSRRTKNNPVLIGDPGVGKTAVVEGLAMRMVAKDVPQNLASASLLS
LDLGALIAGSKYRGEFEERLKSVINELADSGGSVILFIDELHTLVGAGATEGAMDASNLLKPALARGEIHCVGATTLEEY
KKHIEKDAALARRFQPIFVGQPTEEDTISILRGIKEKYEVHHGIRIADSAVVSAAVLSNRYITDRFLPDKAIDLMDEAAS
KIRIESDSKPEVIDELDRKIIQLKIEAEALKKEKDANSIGRLEKIKQDLEGLEKKQADLESSWQAEKSKLKEIQKLKEEL
NEARYSLEIAQRNGDLATAGKILYGTIPALENRLVNEEKQVGGTLLKREVTEDDIANIVAKWTGIPVEKMMGSEQQRLLK
IEDELKKHVIGQDEAVEAVSSAVKRSRAGVQDANRPLGSFMFLGSTGVGKTELSKALAKFLFDNETALLRIDMSEYMEKH
AVARLIGAPPGYVGYEEGGILTEAVRRRPYQVILFDEVEKAHHDVFNILLQVLDEGRLTDTQGHVVDFKNTILILTSNLG
SDVLSEVTQITEVEKTKIMEIVRQFFRPEFLNRLDEILFFNRLSKENIEKILEIQLHKLQERIKTKNLHLSFTHEAKRWI
CEQGYHPVYGARPLKRVIQQYVYNPLAQAILSGSLPENGKIIFDKIGNKLEIQY

Sequences:

>Translated_854_residues
MRFDKFTDNSASIIQSAQLDAVSMGHQTLTSLHILRALLKNTNGIVTQLLEQCGANLDVILNSVELTLRKLPSVSGQGAG
HIHLTPDAAQTIQTSIEIAGRSQDSFVTVERLLQGVAATESEASKILKEQGVDAAKLQQIIDANRGSRRADSANAENNFN
ALRRYTKDLTEIARSGKLDPVIGRDEEIRRVMQVLSRRTKNNPVLIGDPGVGKTAVVEGLAMRMVAKDVPQNLASASLLS
LDLGALIAGSKYRGEFEERLKSVINELADSGGSVILFIDELHTLVGAGATEGAMDASNLLKPALARGEIHCVGATTLEEY
KKHIEKDAALARRFQPIFVGQPTEEDTISILRGIKEKYEVHHGIRIADSAVVSAAVLSNRYITDRFLPDKAIDLMDEAAS
KIRIESDSKPEVIDELDRKIIQLKIEAEALKKEKDANSIGRLEKIKQDLEGLEKKQADLESSWQAEKSKLKEIQKLKEEL
NEARYSLEIAQRNGDLATAGKILYGTIPALENRLVNEEKQVGGTLLKREVTEDDIANIVAKWTGIPVEKMMGSEQQRLLK
IEDELKKHVIGQDEAVEAVSSAVKRSRAGVQDANRPLGSFMFLGSTGVGKTELSKALAKFLFDNETALLRIDMSEYMEKH
AVARLIGAPPGYVGYEEGGILTEAVRRRPYQVILFDEVEKAHHDVFNILLQVLDEGRLTDTQGHVVDFKNTILILTSNLG
SDVLSEVTQITEVEKTKIMEIVRQFFRPEFLNRLDEILFFNRLSKENIEKILEIQLHKLQERIKTKNLHLSFTHEAKRWI
CEQGYHPVYGARPLKRVIQQYVYNPLAQAILSGSLPENGKIIFDKIGNKLEIQY
>Mature_854_residues
MRFDKFTDNSASIIQSAQLDAVSMGHQTLTSLHILRALLKNTNGIVTQLLEQCGANLDVILNSVELTLRKLPSVSGQGAG
HIHLTPDAAQTIQTSIEIAGRSQDSFVTVERLLQGVAATESEASKILKEQGVDAAKLQQIIDANRGSRRADSANAENNFN
ALRRYTKDLTEIARSGKLDPVIGRDEEIRRVMQVLSRRTKNNPVLIGDPGVGKTAVVEGLAMRMVAKDVPQNLASASLLS
LDLGALIAGSKYRGEFEERLKSVINELADSGGSVILFIDELHTLVGAGATEGAMDASNLLKPALARGEIHCVGATTLEEY
KKHIEKDAALARRFQPIFVGQPTEEDTISILRGIKEKYEVHHGIRIADSAVVSAAVLSNRYITDRFLPDKAIDLMDEAAS
KIRIESDSKPEVIDELDRKIIQLKIEAEALKKEKDANSIGRLEKIKQDLEGLEKKQADLESSWQAEKSKLKEIQKLKEEL
NEARYSLEIAQRNGDLATAGKILYGTIPALENRLVNEEKQVGGTLLKREVTEDDIANIVAKWTGIPVEKMMGSEQQRLLK
IEDELKKHVIGQDEAVEAVSSAVKRSRAGVQDANRPLGSFMFLGSTGVGKTELSKALAKFLFDNETALLRIDMSEYMEKH
AVARLIGAPPGYVGYEEGGILTEAVRRRPYQVILFDEVEKAHHDVFNILLQVLDEGRLTDTQGHVVDFKNTILILTSNLG
SDVLSEVTQITEVEKTKIMEIVRQFFRPEFLNRLDEILFFNRLSKENIEKILEIQLHKLQERIKTKNLHLSFTHEAKRWI
CEQGYHPVYGARPLKRVIQQYVYNPLAQAILSGSLPENGKIIFDKIGNKLEIQY

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=310, Percent_Identity=38.0645161290323, Blast_Score=210, Evalue=4e-54,
Organism=Escherichia coli, GI1788943, Length=848, Percent_Identity=54.4811320754717, Blast_Score=924, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=328, Percent_Identity=45.1219512195122, Blast_Score=289, Evalue=5e-79,
Organism=Saccharomyces cerevisiae, GI6320464, Length=691, Percent_Identity=54.8480463096961, Blast_Score=772, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=853, Percent_Identity=41.9695193434936, Blast_Score=651, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 94927; Mature: 94927

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDKFTDNSASIIQSAQLDAVSMGHQTLTSLHILRALLKNTNGIVTQLLEQCGANLDVI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
LNSVELTLRKLPSVSGQGAGHIHLTPDAAQTIQTSIEIAGRSQDSFVTVERLLQGVAATE
HHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCH
SEASKILKEQGVDAAKLQQIIDANRGSRRADSANAENNFNALRRYTKDLTEIARSGKLDP
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
VIGRDEEIRRVMQVLSRRTKNNPVLIGDPGVGKTAVVEGLAMRMVAKDVPQNLASASLLS
CCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDLGALIAGSKYRGEFEERLKSVINELADSGGSVILFIDELHTLVGAGATEGAMDASNLL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHH
KPALARGEIHCVGATTLEEYKKHIEKDAALARRFQPIFVGQPTEEDTISILRGIKEKYEV
HHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHH
HHGIRIADSAVVSAAVLSNRYITDRFLPDKAIDLMDEAASKIRIESDSKPEVIDELDRKI
HCCCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHH
IQLKIEAEALKKEKDANSIGRLEKIKQDLEGLEKKQADLESSWQAEKSKLKEIQKLKEEL
HHHEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEARYSLEIAQRNGDLATAGKILYGTIPALENRLVNEEKQVGGTLLKREVTEDDIANIVA
HHHHHHHHHHHCCCCHHHHCHHHHCCHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHH
KWTGIPVEKMMGSEQQRLLKIEDELKKHVIGQDEAVEAVSSAVKRSRAGVQDANRPLGSF
HHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHE
MFLGSTGVGKTELSKALAKFLFDNETALLRIDMSEYMEKHAVARLIGAPPGYVGYEEGGI
EEECCCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCH
LTEAVRRRPYQVILFDEVEKAHHDVFNILLQVLDEGRLTDTQGHVVDFKNTILILTSNLG
HHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCCC
SDVLSEVTQITEVEKTKIMEIVRQFFRPEFLNRLDEILFFNRLSKENIEKILEIQLHKLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
ERIKTKNLHLSFTHEAKRWICEQGYHPVYGARPLKRVIQQYVYNPLAQAILSGSLPENGK
HHHHHCCCEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IIFDKIGNKLEIQY
EEEECCCCEEEECC
>Mature Secondary Structure
MRFDKFTDNSASIIQSAQLDAVSMGHQTLTSLHILRALLKNTNGIVTQLLEQCGANLDVI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
LNSVELTLRKLPSVSGQGAGHIHLTPDAAQTIQTSIEIAGRSQDSFVTVERLLQGVAATE
HHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCH
SEASKILKEQGVDAAKLQQIIDANRGSRRADSANAENNFNALRRYTKDLTEIARSGKLDP
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
VIGRDEEIRRVMQVLSRRTKNNPVLIGDPGVGKTAVVEGLAMRMVAKDVPQNLASASLLS
CCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDLGALIAGSKYRGEFEERLKSVINELADSGGSVILFIDELHTLVGAGATEGAMDASNLL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHH
KPALARGEIHCVGATTLEEYKKHIEKDAALARRFQPIFVGQPTEEDTISILRGIKEKYEV
HHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHH
HHGIRIADSAVVSAAVLSNRYITDRFLPDKAIDLMDEAASKIRIESDSKPEVIDELDRKI
HCCCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHH
IQLKIEAEALKKEKDANSIGRLEKIKQDLEGLEKKQADLESSWQAEKSKLKEIQKLKEEL
HHHEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEARYSLEIAQRNGDLATAGKILYGTIPALENRLVNEEKQVGGTLLKREVTEDDIANIVA
HHHHHHHHHHHCCCCHHHHCHHHHCCHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHH
KWTGIPVEKMMGSEQQRLLKIEDELKKHVIGQDEAVEAVSSAVKRSRAGVQDANRPLGSF
HHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHE
MFLGSTGVGKTELSKALAKFLFDNETALLRIDMSEYMEKHAVARLIGAPPGYVGYEEGGI
EEECCCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCH
LTEAVRRRPYQVILFDEVEKAHHDVFNILLQVLDEGRLTDTQGHVVDFKNTILILTSNLG
HHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCCC
SDVLSEVTQITEVEKTKIMEIVRQFFRPEFLNRLDEILFFNRLSKENIEKILEIQLHKLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
ERIKTKNLHLSFTHEAKRWICEQGYHPVYGARPLKRVIQQYVYNPLAQAILSGSLPENGK
HHHHHCCCEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IIFDKIGNKLEIQY
EEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA