Click here to switch to the map view.

The map label for this gene is purK

Identifier: 88607982

GI number: 88607982

Start: 851895

End: 852944

Strand: Direct

Name: purK

Synonym: NSE_0965

Alternate gene names: 88607982

Gene position: 851895-852944 (Clockwise)

Preceding gene: 88608316

Following gene: 88608556

Centisome position: 99.17

GC content: 37.9

Gene sequence:

>1050_bases
ATGGACCTTTATAGTAGGAAAATGCAGCGCATTGGAATAATCGGAGGCGGACAGCTAGGAAAAATGACAGCCATAGCTGC
ATATAACCTGGGTTTTAAAGTTTGTGTCCTAGCAGAGAAGGAGAACTCCCCAGCTATAGATGTAGCCAGAGATTATGTTG
TTTCACCTTTCCTCGACAGGTCAGGGATTCTTAACTTTGTAGAACATGTAGACGCTATAACATTTGAGAGCGAAAACATA
CCAACAGAGACGCTTGATCTACTCCACGATAAGTTCGACGTTCCAAATACTAAAGCGATTAAGGTAGCACAGGACAGGTT
CTTAGAAAAAGAATTCTTAAGGAAAAATGGAATACCAACTACCGAATATTGGTACATCGAAAAGGAGGAGGACCTTGATA
GTGTAGATTTTCCAGCAATATTAAAAACAATCAATGGTGGCTATGATGGAAAAGGACAATTCCTCCTAGAGGATCATGAT
TATGTAAGAAGAGAAGCCGGAAACCTCAAATTCCCTCTAATAGCAGAAAAGTTATTTAGGATAAGTAAAGAATTCTCCAT
AATAGTTTCGAGAAATGAAACTGGAAGTGTGTGTTTTCCGATAGCAGAAAATGTTCATGTTAATGGAATACTCAAAACAT
CCAGCGTGCCAGCTGTACTTCCTCATCATGTCGCACTTGAAATAAAAAACATAGGATTTCAAATAGCAGATCTATTAGAA
ATAAAGGGTCTCTTGTGTGTAGAATTTTTTCTCGACGAAGATAACAAGCTAGTTGTGAATGAAATCGCTCCTAGACCACA
CAATTCTGGTCACTGGAGCATGGATTGCTGCGACATTGACCAATTTGAAGAACTGGTTCTTGCAATTACAGGTAATAAAC
TCAAAAAGCCTAATCTCGTAGTTCCGTGCACAATGAAGAATATTCTTGGCAATGAAATAAATACTTGGAAAGATTTATTC
CTACAAAAAAATGTAAAACTCTACAACTATGGTAAAGAACAGCCTAAAATCCTAAGGAAAATGGGGCATATAAACATTCT
GCATCCGTAA

Upstream 100 bases:

>100_bases
TGAACTAGTAAGTTTCAATGACCTGAAAACAAGTAGCTATCCTTTTGCATTAAGGTAGTTCGTATTCAGATTAGTTTTGG
TGTAACTATAAATAAAATAG

Downstream 100 bases:

>100_bases
GACATCCTCAAGTGTTTGTTTTACTTGATTCATGTAAGAGAGAACAAGCATCCTAAGAAAGATGTAACTTACAAACATTT
TACGTCCGCAAGATATTCTC

Product: phosphoribosylaminoimidazole carboxylase, ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MDLYSRKMQRIGIIGGGQLGKMTAIAAYNLGFKVCVLAEKENSPAIDVARDYVVSPFLDRSGILNFVEHVDAITFESENI
PTETLDLLHDKFDVPNTKAIKVAQDRFLEKEFLRKNGIPTTEYWYIEKEEDLDSVDFPAILKTINGGYDGKGQFLLEDHD
YVRREAGNLKFPLIAEKLFRISKEFSIIVSRNETGSVCFPIAENVHVNGILKTSSVPAVLPHHVALEIKNIGFQIADLLE
IKGLLCVEFFLDEDNKLVVNEIAPRPHNSGHWSMDCCDIDQFEELVLAITGNKLKKPNLVVPCTMKNILGNEINTWKDLF
LQKNVKLYNYGKEQPKILRKMGHINILHP

Sequences:

>Translated_349_residues
MDLYSRKMQRIGIIGGGQLGKMTAIAAYNLGFKVCVLAEKENSPAIDVARDYVVSPFLDRSGILNFVEHVDAITFESENI
PTETLDLLHDKFDVPNTKAIKVAQDRFLEKEFLRKNGIPTTEYWYIEKEEDLDSVDFPAILKTINGGYDGKGQFLLEDHD
YVRREAGNLKFPLIAEKLFRISKEFSIIVSRNETGSVCFPIAENVHVNGILKTSSVPAVLPHHVALEIKNIGFQIADLLE
IKGLLCVEFFLDEDNKLVVNEIAPRPHNSGHWSMDCCDIDQFEELVLAITGNKLKKPNLVVPCTMKNILGNEINTWKDLF
LQKNVKLYNYGKEQPKILRKMGHINILHP
>Mature_349_residues
MDLYSRKMQRIGIIGGGQLGKMTAIAAYNLGFKVCVLAEKENSPAIDVARDYVVSPFLDRSGILNFVEHVDAITFESENI
PTETLDLLHDKFDVPNTKAIKVAQDRFLEKEFLRKNGIPTTEYWYIEKEEDLDSVDFPAILKTINGGYDGKGQFLLEDHD
YVRREAGNLKFPLIAEKLFRISKEFSIIVSRNETGSVCFPIAENVHVNGILKTSSVPAVLPHHVALEIKNIGFQIADLLE
IKGLLCVEFFLDEDNKLVVNEIAPRPHNSGHWSMDCCDIDQFEELVLAITGNKLKKPNLVVPCTMKNILGNEINTWKDLF
LQKNVKLYNYGKEQPKILRKMGHINILHP

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=349, Percent_Identity=29.7994269340974, Blast_Score=122, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324702, Length=364, Percent_Identity=33.7912087912088, Blast_Score=179, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 39526; Mature: 39526

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLYSRKMQRIGIIGGGQLGKMTAIAAYNLGFKVCVLAEKENSPAIDVARDYVVSPFLDR
CCHHHHHHHHEEEECCCCCCCEEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHCCCCCH
SGILNFVEHVDAITFESENIPTETLDLLHDKFDVPNTKAIKVAQDRFLEKEFLRKNGIPT
HHHHHHHHHHHHEEECCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCC
TEYWYIEKEEDLDSVDFPAILKTINGGYDGKGQFLLEDHDYVRREAGNLKFPLIAEKLFR
CEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCEEECCCHHHHHHCCCCCCHHHHHHHHH
ISKEFSIIVSRNETGSVCFPIAENVHVNGILKTSSVPAVLPHHVALEIKNIGFQIADLLE
HCCCEEEEEECCCCCCEEEEECCCEEEEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHH
IKGLLCVEFFLDEDNKLVVNEIAPRPHNSGHWSMDCCDIDQFEELVLAITGNKLKKPNLV
HHHHEEEEEEECCCCEEEEEECCCCCCCCCCCEEEECCHHHHHHEEEEEECCCCCCCCEE
VPCTMKNILGNEINTWKDLFLQKNVKLYNYGKEQPKILRKMGHINILHP
EEECHHHHHCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHCCCEEECCC
>Mature Secondary Structure
MDLYSRKMQRIGIIGGGQLGKMTAIAAYNLGFKVCVLAEKENSPAIDVARDYVVSPFLDR
CCHHHHHHHHEEEECCCCCCCEEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHCCCCCH
SGILNFVEHVDAITFESENIPTETLDLLHDKFDVPNTKAIKVAQDRFLEKEFLRKNGIPT
HHHHHHHHHHHHEEECCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCC
TEYWYIEKEEDLDSVDFPAILKTINGGYDGKGQFLLEDHDYVRREAGNLKFPLIAEKLFR
CEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCEEECCCHHHHHHCCCCCCHHHHHHHHH
ISKEFSIIVSRNETGSVCFPIAENVHVNGILKTSSVPAVLPHHVALEIKNIGFQIADLLE
HCCCEEEEEECCCCCCEEEEECCCEEEEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHH
IKGLLCVEFFLDEDNKLVVNEIAPRPHNSGHWSMDCCDIDQFEELVLAITGNKLKKPNLV
HHHHEEEEEEECCCCEEEEEECCCCCCCCCCCEEEECCHHHHHHEEEEEECCCCCCCCEE
VPCTMKNILGNEINTWKDLFLQKNVKLYNYGKEQPKILRKMGHINILHP
EEECHHHHHCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]