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The map label for this gene is mdh

Identifier: 88607793

GI number: 88607793

Start: 654397

End: 655362

Strand: Direct

Name: mdh

Synonym: APH_0629

Alternate gene names: 88607793

Gene position: 654397-655362 (Clockwise)

Preceding gene: 88607213

Following gene: 88607811

Centisome position: 44.48

GC content: 44.41

Gene sequence:

>966_bases
ATGCGGTCTCATCGTTCAGTAAAAGTTTCATTAGTAGGTGCTGGGAATATAGGTGGTACTCTAGCCTATATGCTGGGTGT
TGCGGGTATCTGTCAGGAGCTGGTATTCGTAGATGTGATGGACGGTGTGCCACGGGGGAAGTTATTGGATATTGGGCATG
CGCTAGCAATATCGGGTGTGGATATAACAGCAGTTGGTGGTTCAGACTATGCAGCTATTGAGGGATCTGATGCGATTGTG
GTTACTGCAGGCCTTCCTAGAAAGGAAGGGATGAGTCGTGAAGATTTGCTCATGGCTAATGCAGCAGTGATAAAAGGTGT
GGCCGAAAATATTAGGAAATATTCTCCCGATGCTTTTGTTATAGTTGTGACTAATCCTCTTGATGCTATGGTGTGGTATA
TGCATCAATGTTCAGGATTGCCTGTGAACAAAGTAGTTGGCATGGCGGGCGTTCTGGATTCAGCGAGGTTTTCTTTTTTC
CTTGCTAAGCACATGAGTGTTTCGGTTAGTAGCGTGTCATCTGTTGTCTTAGGTGGGCACGGTGACCTTATGTTGCCTTT
GTTGAAGTATTCTACTGTTGGAGGTGTTTCTGTTTCAGACCTTATAAGCTGTGGTCGTCTGAGTTCTGAGGATGTGCATG
CAATTATAGAAAGGACTCGCAAGGGAGGAGAGGAGATTGTTAAATTGCTAAAGAGTGGTTCAGCGTATTATGCTCCGGCT
GCTTCTTGTATGAATATGCTAGAATCATACTTATTCGATAAGCGCTGTGTGATCCCTTGTTCAGTAGGGCTGGATGGTAA
ATATGGGGTGAATGGCGGTCTGTTTGTGGGGGTTCCAGCGGTTATAGGGAAGAATGGCGTAGAAGAGGTGATAGAATACG
TTCTTTCACAAGAAGAGCGGGAAATTTTCGAAAAATCAGTGGGATTAATCAGTAATTCAGTGAAGATTATTTCTGAGCAA
AAGTAG

Upstream 100 bases:

>100_bases
CTATCTCTGCTATATGGAATAAGGACAGGTAGGGAAGCCTGCTTGACAAGTATAGAAGGCCTGCTATACTCCGAGGGTTT
TTGTTTTTCACATTGTGGCT

Downstream 100 bases:

>100_bases
TATTGTATCTTACGTGACAGAATGCATTCGGGTGGGTGATGTTTCATCTTGTTTGGGATGAAATAAGACCGGTGTTGTGT
ATTATGCTCTCGCGATGTGG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MRSHRSVKVSLVGAGNIGGTLAYMLGVAGICQELVFVDVMDGVPRGKLLDIGHALAISGVDITAVGGSDYAAIEGSDAIV
VTAGLPRKEGMSREDLLMANAAVIKGVAENIRKYSPDAFVIVVTNPLDAMVWYMHQCSGLPVNKVVGMAGVLDSARFSFF
LAKHMSVSVSSVSSVVLGGHGDLMLPLLKYSTVGGVSVSDLISCGRLSSEDVHAIIERTRKGGEEIVKLLKSGSAYYAPA
ASCMNMLESYLFDKRCVIPCSVGLDGKYGVNGGLFVGVPAVIGKNGVEEVIEYVLSQEEREIFEKSVGLISNSVKIISEQ
K

Sequences:

>Translated_321_residues
MRSHRSVKVSLVGAGNIGGTLAYMLGVAGICQELVFVDVMDGVPRGKLLDIGHALAISGVDITAVGGSDYAAIEGSDAIV
VTAGLPRKEGMSREDLLMANAAVIKGVAENIRKYSPDAFVIVVTNPLDAMVWYMHQCSGLPVNKVVGMAGVLDSARFSFF
LAKHMSVSVSSVSSVVLGGHGDLMLPLLKYSTVGGVSVSDLISCGRLSSEDVHAIIERTRKGGEEIVKLLKSGSAYYAPA
ASCMNMLESYLFDKRCVIPCSVGLDGKYGVNGGLFVGVPAVIGKNGVEEVIEYVLSQEEREIFEKSVGLISNSVKIISEQ
K
>Mature_321_residues
MRSHRSVKVSLVGAGNIGGTLAYMLGVAGICQELVFVDVMDGVPRGKLLDIGHALAISGVDITAVGGSDYAAIEGSDAIV
VTAGLPRKEGMSREDLLMANAAVIKGVAENIRKYSPDAFVIVVTNPLDAMVWYMHQCSGLPVNKVVGMAGVLDSARFSFF
LAKHMSVSVSSVSSVVLGGHGDLMLPLLKYSTVGGVSVSDLISCGRLSSEDVHAIIERTRKGGEEIVKLLKSGSAYYAPA
ASCMNMLESYLFDKRCVIPCSVGLDGKYGVNGGLFVGVPAVIGKNGVEEVIEYVLSQEEREIFEKSVGLISNSVKIISEQ
K

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=302, Percent_Identity=32.1192052980132, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI221136809, Length=302, Percent_Identity=32.1192052980132, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI9257228, Length=308, Percent_Identity=33.7662337662338, Blast_Score=167, Evalue=8e-42,
Organism=Homo sapiens, GI4504973, Length=308, Percent_Identity=33.7662337662338, Blast_Score=167, Evalue=8e-42,
Organism=Homo sapiens, GI5031857, Length=305, Percent_Identity=30.8196721311475, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI260099723, Length=305, Percent_Identity=30.8196721311475, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI291575128, Length=305, Percent_Identity=31.1475409836066, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI4557032, Length=305, Percent_Identity=31.1475409836066, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI15082234, Length=321, Percent_Identity=28.9719626168224, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI260099727, Length=195, Percent_Identity=33.8461538461538, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI260099725, Length=195, Percent_Identity=33.8461538461538, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI207028494, Length=190, Percent_Identity=29.4736842105263, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI103472011, Length=291, Percent_Identity=22.680412371134, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI21735621, Length=316, Percent_Identity=26.2658227848101, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1789632, Length=297, Percent_Identity=30.976430976431, Blast_Score=95, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17535107, Length=288, Percent_Identity=29.8611111111111, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17554310, Length=325, Percent_Identity=29.2307692307692, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322765, Length=295, Percent_Identity=27.4576271186441, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324446, Length=252, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320125, Length=274, Percent_Identity=26.2773722627737, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17136226, Length=309, Percent_Identity=29.4498381877023, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI45550422, Length=314, Percent_Identity=23.8853503184713, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24647881, Length=328, Percent_Identity=28.3536585365854, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24663599, Length=324, Percent_Identity=26.2345679012346, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24663595, Length=253, Percent_Identity=26.8774703557312, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_ANAPZ (Q2GK85)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505216.1
- ProteinModelPortal:   Q2GK85
- SMR:   Q2GK85
- STRING:   Q2GK85
- GeneID:   3930971
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0629
- NMPDR:   fig|212042.5.peg.616
- TIGR:   APH_0629
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   DSARFSF
- PhylomeDB:   Q2GK85
- ProtClustDB:   PRK06223
- BioCyc:   APHA212042:APH_0629-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33836; Mature: 33836

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: ACT_SITE 180-180 BINDING 38-38 BINDING 87-87 BINDING 93-93 BINDING 100-100 BINDING 125-125 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSHRSVKVSLVGAGNIGGTLAYMLGVAGICQELVFVDVMDGVPRGKLLDIGHALAISGV
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEECCC
DITAVGGSDYAAIEGSDAIVVTAGLPRKEGMSREDLLMANAAVIKGVAENIRKYSPDAFV
EEEEECCCCEEEEECCCEEEEEECCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IVVTNPLDAMVWYMHQCSGLPVNKVVGMAGVLDSARFSFFLAKHMSVSVSSVSSVVLGGH
EEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCC
GDLMLPLLKYSTVGGVSVSDLISCGRLSSEDVHAIIERTRKGGEEIVKLLKSGSAYYAPA
CCHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCH
ASCMNMLESYLFDKRCVIPCSVGLDGKYGVNGGLFVGVPAVIGKNGVEEVIEYVLSQEER
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEECHHHHCCCCHHHHHHHHHCHHHH
EIFEKSVGLISNSVKIISEQK
HHHHHHHHHHHCCHHEEECCC
>Mature Secondary Structure
MRSHRSVKVSLVGAGNIGGTLAYMLGVAGICQELVFVDVMDGVPRGKLLDIGHALAISGV
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEECCC
DITAVGGSDYAAIEGSDAIVVTAGLPRKEGMSREDLLMANAAVIKGVAENIRKYSPDAFV
EEEEECCCCEEEEECCCEEEEEECCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IVVTNPLDAMVWYMHQCSGLPVNKVVGMAGVLDSARFSFFLAKHMSVSVSSVSSVVLGGH
EEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCC
GDLMLPLLKYSTVGGVSVSDLISCGRLSSEDVHAIIERTRKGGEEIVKLLKSGSAYYAPA
CCHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCH
ASCMNMLESYLFDKRCVIPCSVGLDGKYGVNGGLFVGVPAVIGKNGVEEVIEYVLSQEER
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEECHHHHCCCCHHHHHHHHHCHHHH
EIFEKSVGLISNSVKIISEQK
HHHHHHHHHHHCCHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA