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The map label for this gene is purH [H]

Identifier: 88607678

GI number: 88607678

Start: 1274876

End: 1276408

Strand: Reverse

Name: purH [H]

Synonym: APH_1207

Alternate gene names: 88607678

Gene position: 1276408-1274876 (Counterclockwise)

Preceding gene: 88607258

Following gene: 88607007

Centisome position: 86.75

GC content: 45.14

Gene sequence:

>1533_bases
ATGCAGAAAACAAAAGTGGCTCTTTTATCCGTAACGGATAAGGAGGGAGTAGAGGAGTTGGCTCGCTTTTTGGTGAAAAA
CGGTTTTCGTATCCTTGCTACAAAAAATACAAATCTTTTGCTGAGGGATTCTGGCATAGAGTCAACAGAGGTGTCGGAAT
ACACAGAGTATGATGAAATTATGGGAGGGCGTGTTAAGACGCTGCATCCGAAGATTTTTGCTGGAATTTTGTGCAACCGT
GGGTCGCACATGCAGGAGGGAGAGAGGCTTGGTATTGATAATATAGATCTCCTTGTGGTGAATTTGTATCCTTTTGCGCA
GTGTGTGGCTCGTGCTGATGCTACGGAGCATGATATTATAGAAAATATTGATATAGGTGGTGTGGCGCTTTTAAGGGCTG
GTGCGAAGAATTTTCAACATGTCACTGTTGTTTTGAGTGCTCAGGACTATGATGAGTTGAAGGGCGAGATTGAGAATAAT
GGGGGCAATACTTCCCTTGAGTATCGTAAAAAGATGGCTGCTAGGGCTTTTGCATTGACTGCTGCTTATGATGCGAGGGT
GCATAGCTGGTTGTTGTGTGATAAGCAGGATGAGGTATTACCGAGTGAGATCGTTATACATGGGAAGAAGGTTCAGGATC
TGCGTATAGGTGAGAATCCGCATCAAAAGGCTGCGCTTTATTGTGTGGGAAGTTATGGTGATGCGCTTCCTATAGAGCAA
ATACATGGGAAAGATCTGAGTTATAATAATATCGTTGATATAGAATCTGCCATAAAGATAGTGGCAGACTTTGATGTTCC
TGCGGTTTCGGTTATCAAGCATGGTAATCCTTGCGGGGTTGCCATTTCTAGTGAAGGTATTGACGATGCGTATGAGAAGG
CTATTTCGTGCGATCCACGTAGTAGTTTTGGGGGCATAATAGCGCTAAACAAGGCCATGACCATGGATATTGCTAGAAAG
GTCATGAGTATATTTATAGAAGCTGTAGTAGCGCCTGACTTTGATGATGGGGTGATAGATACTCTAACAGCGAAGAAGAA
CATGCGCATTATGAGGTATAAGCCGTATACTCCTGATAGGTTAGTGGTAAAGAGCACATTGTCTGGTGGGTTGTTGGTGC
AGGAGTGCGATCGTAGCGTGGTTAATATAAGTGATTTCAAGTTGGCTACGGAGGTTAGTGCTTCTCCTGAGGTGTTAAAT
GATCTTTTGTTTGCTTGGAGAGTGTGTAAGTACGTACGCTCGAATGCGATAGTGATCGCACGAGATGGTCGCGCAATTGG
TGTAGGGGCAGGGCAAATGAGCCGTGTTGATAGTGTGGAAATAGCGATACGTAAGGCTGGGAATTGTGCAGGTGCGGTTT
TAGCTTCTGATGCCTTTTTTCCGTTTGCGGATAGTATTCAGCATGCTGTTTCTGCTGGTATAGCGGCTATAGTGCAGCCA
GGGGGCTCGCTGCGGGATCAGGAAGTCATAGATGCGGCGAATGCCAGTGGTATACCTATGTATTTCACGGGAGTGCGCGG
TTTTTGCCATTAG

Upstream 100 bases:

>100_bases
ACAGCTGTAGGTTTGACTGTCGTGCTTATGTAGAAGATGTCTTGGTGACTGAGGCGCAGATCTTGGCAATGATGAATAAA
TAGTGGATAAAGCAATGGAT

Downstream 100 bases:

>100_bases
AGAGTTGTCTAGTGCGCCATCAAATTGATGGGGAGCGGAGCTATTGCCTTGGTTAATGTTGGGGTTCTGTAGATTGTTTC
GGCTAACAGTTTGATTGAAA

Product: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MQKTKVALLSVTDKEGVEELARFLVKNGFRILATKNTNLLLRDSGIESTEVSEYTEYDEIMGGRVKTLHPKIFAGILCNR
GSHMQEGERLGIDNIDLLVVNLYPFAQCVARADATEHDIIENIDIGGVALLRAGAKNFQHVTVVLSAQDYDELKGEIENN
GGNTSLEYRKKMAARAFALTAAYDARVHSWLLCDKQDEVLPSEIVIHGKKVQDLRIGENPHQKAALYCVGSYGDALPIEQ
IHGKDLSYNNIVDIESAIKIVADFDVPAVSVIKHGNPCGVAISSEGIDDAYEKAISCDPRSSFGGIIALNKAMTMDIARK
VMSIFIEAVVAPDFDDGVIDTLTAKKNMRIMRYKPYTPDRLVVKSTLSGGLLVQECDRSVVNISDFKLATEVSASPEVLN
DLLFAWRVCKYVRSNAIVIARDGRAIGVGAGQMSRVDSVEIAIRKAGNCAGAVLASDAFFPFADSIQHAVSAGIAAIVQP
GGSLRDQEVIDAANASGIPMYFTGVRGFCH

Sequences:

>Translated_510_residues
MQKTKVALLSVTDKEGVEELARFLVKNGFRILATKNTNLLLRDSGIESTEVSEYTEYDEIMGGRVKTLHPKIFAGILCNR
GSHMQEGERLGIDNIDLLVVNLYPFAQCVARADATEHDIIENIDIGGVALLRAGAKNFQHVTVVLSAQDYDELKGEIENN
GGNTSLEYRKKMAARAFALTAAYDARVHSWLLCDKQDEVLPSEIVIHGKKVQDLRIGENPHQKAALYCVGSYGDALPIEQ
IHGKDLSYNNIVDIESAIKIVADFDVPAVSVIKHGNPCGVAISSEGIDDAYEKAISCDPRSSFGGIIALNKAMTMDIARK
VMSIFIEAVVAPDFDDGVIDTLTAKKNMRIMRYKPYTPDRLVVKSTLSGGLLVQECDRSVVNISDFKLATEVSASPEVLN
DLLFAWRVCKYVRSNAIVIARDGRAIGVGAGQMSRVDSVEIAIRKAGNCAGAVLASDAFFPFADSIQHAVSAGIAAIVQP
GGSLRDQEVIDAANASGIPMYFTGVRGFCH
>Mature_510_residues
MQKTKVALLSVTDKEGVEELARFLVKNGFRILATKNTNLLLRDSGIESTEVSEYTEYDEIMGGRVKTLHPKIFAGILCNR
GSHMQEGERLGIDNIDLLVVNLYPFAQCVARADATEHDIIENIDIGGVALLRAGAKNFQHVTVVLSAQDYDELKGEIENN
GGNTSLEYRKKMAARAFALTAAYDARVHSWLLCDKQDEVLPSEIVIHGKKVQDLRIGENPHQKAALYCVGSYGDALPIEQ
IHGKDLSYNNIVDIESAIKIVADFDVPAVSVIKHGNPCGVAISSEGIDDAYEKAISCDPRSSFGGIIALNKAMTMDIARK
VMSIFIEAVVAPDFDDGVIDTLTAKKNMRIMRYKPYTPDRLVVKSTLSGGLLVQECDRSVVNISDFKLATEVSASPEVLN
DLLFAWRVCKYVRSNAIVIARDGRAIGVGAGQMSRVDSVEIAIRKAGNCAGAVLASDAFFPFADSIQHAVSAGIAAIVQP
GGSLRDQEVIDAANASGIPMYFTGVRGFCH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=477, Percent_Identity=34.5911949685535, Blast_Score=223, Evalue=3e-58,
Organism=Escherichia coli, GI1790439, Length=523, Percent_Identity=43.0210325047801, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71985564, Length=500, Percent_Identity=31.8, Blast_Score=218, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI71985574, Length=330, Percent_Identity=27.2727272727273, Blast_Score=95, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6323768, Length=473, Percent_Identity=34.0380549682875, Blast_Score=226, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6323056, Length=473, Percent_Identity=32.9809725158562, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24649832, Length=479, Percent_Identity=36.1169102296451, Blast_Score=244, Evalue=1e-64,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55298; Mature: 55298

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTKVALLSVTDKEGVEELARFLVKNGFRILATKNTNLLLRDSGIESTEVSEYTEYDEI
CCCCEEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHH
MGGRVKTLHPKIFAGILCNRGSHMQEGERLGIDNIDLLVVNLYPFAQCVARADATEHDII
HCCCEEEECHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCHHHHH
ENIDIGGVALLRAGAKNFQHVTVVLSAQDYDELKGEIENNGGNTSLEYRKKMAARAFALT
HCCCCCHHHHHHHCCCCCEEEEEEEECCCHHHHCCEEECCCCCCHHHHHHHHHHHHHHHH
AAYDARVHSWLLCDKQDEVLPSEIVIHGKKVQDLRIGENPHQKAALYCVGSYGDALPIEQ
HHHHHHHHEEEEECCCCCCCCCCEEEECCEEEEEECCCCCCCCEEEEEEECCCCCCCHHH
IHGKDLSYNNIVDIESAIKIVADFDVPAVSVIKHGNPCGVAISSEGIDDAYEKAISCDPR
HCCCCCCCCCEEEHHHHEEEEECCCCCEEEEEECCCCCEEEEECCCCCHHHHHHCCCCCC
SSFGGIIALNKAMTMDIARKVMSIFIEAVVAPDFDDGVIDTLTAKKNMRIMRYKPYTPDR
CCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEECCCCCCC
LVVKSTLSGGLLVQECDRSVVNISDFKLATEVSASPEVLNDLLFAWRVCKYVRSNAIVIA
EEEEECCCCCEEHHHHCCCCEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEE
RDGRAIGVGAGQMSRVDSVEIAIRKAGNCAGAVLASDAFFPFADSIQHAVSAGIAAIVQP
ECCCEEEECCCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCHHHHHHHHHHCEEEEECC
GGSLRDQEVIDAANASGIPMYFTGVRGFCH
CCCCCCHHHHHHCCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MQKTKVALLSVTDKEGVEELARFLVKNGFRILATKNTNLLLRDSGIESTEVSEYTEYDEI
CCCCEEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHH
MGGRVKTLHPKIFAGILCNRGSHMQEGERLGIDNIDLLVVNLYPFAQCVARADATEHDII
HCCCEEEECHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCHHHHH
ENIDIGGVALLRAGAKNFQHVTVVLSAQDYDELKGEIENNGGNTSLEYRKKMAARAFALT
HCCCCCHHHHHHHCCCCCEEEEEEEECCCHHHHCCEEECCCCCCHHHHHHHHHHHHHHHH
AAYDARVHSWLLCDKQDEVLPSEIVIHGKKVQDLRIGENPHQKAALYCVGSYGDALPIEQ
HHHHHHHHEEEEECCCCCCCCCCEEEECCEEEEEECCCCCCCCEEEEEEECCCCCCCHHH
IHGKDLSYNNIVDIESAIKIVADFDVPAVSVIKHGNPCGVAISSEGIDDAYEKAISCDPR
HCCCCCCCCCEEEHHHHEEEEECCCCCEEEEEECCCCCEEEEECCCCCHHHHHHCCCCCC
SSFGGIIALNKAMTMDIARKVMSIFIEAVVAPDFDDGVIDTLTAKKNMRIMRYKPYTPDR
CCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEECCCCCCC
LVVKSTLSGGLLVQECDRSVVNISDFKLATEVSASPEVLNDLLFAWRVCKYVRSNAIVIA
EEEEECCCCCEEHHHHCCCCEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEE
RDGRAIGVGAGQMSRVDSVEIAIRKAGNCAGAVLASDAFFPFADSIQHAVSAGIAAIVQP
ECCCEEEECCCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCHHHHHHHHHHCEEEEECC
GGSLRDQEVIDAANASGIPMYFTGVRGFCH
CCCCCCHHHHHHCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA