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The map label for this gene is tolB [H]

Identifier: 88607556

GI number: 88607556

Start: 1101089

End: 1102396

Strand: Direct

Name: tolB [H]

Synonym: APH_1037

Alternate gene names: 88607556

Gene position: 1101089-1102396 (Clockwise)

Preceding gene: 88607873

Following gene: 88607133

Centisome position: 74.84

GC content: 43.5

Gene sequence:

>1308_bases
ATGTCAATTAGGCTTGGAGTTGCTACGTTTTTCATATTTTTATTATTCTGTTGTGGGGGTGCTGTTGCCTCTTTGCGTCT
AAGTATCACTAAGGGCAACACTGGGAAACTAAGTATAGCTTTTGCGCCCTTGCACTCTTTTGAGAGCAATTTAGCGCAGA
AGATTGGCGAGAGTTGCACAGATATCATCTTAAAGGACTTGAACAACACAGGGTTATTTGATGCGCGTATTGAGATTCTT
GATCCAACCTCAGATGGCGCAGCTTACTCCAATGTATGGAGAGAATTGAAGCATGACATCCTGCTTACAGGCAATGTGCA
GGAGCTTTCACACGGAAGAATGGGTATCAAGTTATTCGTGTGGGATGTAGCTGCGGAAAAGAAATTGTCTGGCAAGTCTT
TTAACTTTGAAATAGGAGGGTGGCGTCGGGCTGCTCATACCGTTGCAGATCATATTTATACCAGGATTACTGGCAATGGC
GGGTACTTCGACTCAAAAATTGCGTACATTGCGGATATGCTGGGTGAGAGAAGAATAGCTCTGATGGATTATGATGGAGC
TAACAGTGTTTACGCTTCTATAGATAATAATGGATGGCTATCTTTCCCTAGATTTTCTCCTGATACAAAAAGCATAGTGT
ACACTCTCACATCGCAGTCTTCTCCTTCTGGGAGAGTTATGTTGTACGATATGAGGAGAGAGGTTTCTTCGCTGCTAATA
GGCATGTCTGGTATTGTGTCGTCGGTGAGATTTTCGCCAAGTGGCCGCGAAATATTGCTATCAGAATCGCTTAATGGCAG
CACTGACATATACTCCTTTAGTCTTATAAGCAAAAAACTTACGAGGCTGACCGCTGATGAGTTCATGAACACGTCTGCTT
CTTATTCCCCTGATGAGAAATCTATAGTATTTGCTTCGGACCGAGACGGTGGATCTCATCTCTATATTATGGATGCTAAT
GGAAGTAACCCACGCAAAATAAGCTCAGGCTCAGGCAGGTACATGTCTCCTGTATGGTCTCCCAACGGAACTTGGATAGC
ATTTACTAAGGTCAAGGGTGCTCGTTGTTATATTGGGATCATGCGTCCTAATGGTAGTAAGGAAAGAATGATAGCGTCGG
ATTGCTTTGCTGAAGGACCGTCTTGGGCGCCGAATGGTCAGGCAGTTTTATATACTAAACATGTTCAAAGAGTGGAGCAT
TTGGCCACAGAACTTGCTATGGTAGACTTATCAAGCATGAATAGGAGGGCTATAAATACTCCAAGTGACGCCTTGATCGC
ACATTGGTCGAATACAATGCAAAATTAG

Upstream 100 bases:

>100_bases
CGTAGACCTAAAAACATAAAGGGTTATATAAGAAATTTGGTTTTTGCTTTTCTTAAAGACGTGGTAAAGGGCAAACCATT
GGTGGAAGTACAGTTGGAAT

Downstream 100 bases:

>100_bases
AAGTAATAAACGTTATTCCTGACACGGCATTACACTCGGTGGGTATATTTCAACGTATACCTTCTAACGTCATAGTGTGA
TACATATGAGAATAGTGGAC

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSIRLGVATFFIFLLFCCGGAVASLRLSITKGNTGKLSIAFAPLHSFESNLAQKIGESCTDIILKDLNNTGLFDARIEIL
DPTSDGAAYSNVWRELKHDILLTGNVQELSHGRMGIKLFVWDVAAEKKLSGKSFNFEIGGWRRAAHTVADHIYTRITGNG
GYFDSKIAYIADMLGERRIALMDYDGANSVYASIDNNGWLSFPRFSPDTKSIVYTLTSQSSPSGRVMLYDMRREVSSLLI
GMSGIVSSVRFSPSGREILLSESLNGSTDIYSFSLISKKLTRLTADEFMNTSASYSPDEKSIVFASDRDGGSHLYIMDAN
GSNPRKISSGSGRYMSPVWSPNGTWIAFTKVKGARCYIGIMRPNGSKERMIASDCFAEGPSWAPNGQAVLYTKHVQRVEH
LATELAMVDLSSMNRRAINTPSDALIAHWSNTMQN

Sequences:

>Translated_435_residues
MSIRLGVATFFIFLLFCCGGAVASLRLSITKGNTGKLSIAFAPLHSFESNLAQKIGESCTDIILKDLNNTGLFDARIEIL
DPTSDGAAYSNVWRELKHDILLTGNVQELSHGRMGIKLFVWDVAAEKKLSGKSFNFEIGGWRRAAHTVADHIYTRITGNG
GYFDSKIAYIADMLGERRIALMDYDGANSVYASIDNNGWLSFPRFSPDTKSIVYTLTSQSSPSGRVMLYDMRREVSSLLI
GMSGIVSSVRFSPSGREILLSESLNGSTDIYSFSLISKKLTRLTADEFMNTSASYSPDEKSIVFASDRDGGSHLYIMDAN
GSNPRKISSGSGRYMSPVWSPNGTWIAFTKVKGARCYIGIMRPNGSKERMIASDCFAEGPSWAPNGQAVLYTKHVQRVEH
LATELAMVDLSSMNRRAINTPSDALIAHWSNTMQN
>Mature_434_residues
SIRLGVATFFIFLLFCCGGAVASLRLSITKGNTGKLSIAFAPLHSFESNLAQKIGESCTDIILKDLNNTGLFDARIEILD
PTSDGAAYSNVWRELKHDILLTGNVQELSHGRMGIKLFVWDVAAEKKLSGKSFNFEIGGWRRAAHTVADHIYTRITGNGG
YFDSKIAYIADMLGERRIALMDYDGANSVYASIDNNGWLSFPRFSPDTKSIVYTLTSQSSPSGRVMLYDMRREVSSLLIG
MSGIVSSVRFSPSGREILLSESLNGSTDIYSFSLISKKLTRLTADEFMNTSASYSPDEKSIVFASDRDGGSHLYIMDANG
SNPRKISSGSGRYMSPVWSPNGTWIAFTKVKGARCYIGIMRPNGSKERMIASDCFAEGPSWAPNGQAVLYTKHVQRVEHL
ATELAMVDLSSMNRRAINTPSDALIAHWSNTMQN

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=397, Percent_Identity=25.1889168765743, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 47801; Mature: 47670

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRLGVATFFIFLLFCCGGAVASLRLSITKGNTGKLSIAFAPLHSFESNLAQKIGESCT
CEEEEHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHH
DIILKDLNNTGLFDARIEILDPTSDGAAYSNVWRELKHDILLTGNVQELSHGRMGIKLFV
HHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCEEEECCHHHHCCCCCEEEEEE
WDVAAEKKLSGKSFNFEIGGWRRAAHTVADHIYTRITGNGGYFDSKIAYIADMLGERRIA
EEECCCCCCCCCCEEEECCCHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCEEE
LMDYDGANSVYASIDNNGWLSFPRFSPDTKSIVYTLTSQSSPSGRVMLYDMRREVSSLLI
EEECCCCCCEEEEECCCCCEECCCCCCCCCEEEEEEECCCCCCCCEEEEHHHHHHHHHHH
GMSGIVSSVRFSPSGREILLSESLNGSTDIYSFSLISKKLTRLTADEFMNTSASYSPDEK
HHHHHHHHEEECCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SIVFASDRDGGSHLYIMDANGSNPRKISSGSGRYMSPVWSPNGTWIAFTKVKGARCYIGI
EEEEEECCCCCCEEEEEECCCCCCCEECCCCCCEECCEECCCCCEEEEEEECCCEEEEEE
MRPNGSKERMIASDCFAEGPSWAPNGQAVLYTKHVQRVEHLATELAMVDLSSMNRRAINT
ECCCCCCCCEEHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PSDALIAHWSNTMQN
CCHHEEEECHHHHCC
>Mature Secondary Structure 
SIRLGVATFFIFLLFCCGGAVASLRLSITKGNTGKLSIAFAPLHSFESNLAQKIGESCT
EEEEHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHH
DIILKDLNNTGLFDARIEILDPTSDGAAYSNVWRELKHDILLTGNVQELSHGRMGIKLFV
HHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCEEEECCHHHHCCCCCEEEEEE
WDVAAEKKLSGKSFNFEIGGWRRAAHTVADHIYTRITGNGGYFDSKIAYIADMLGERRIA
EEECCCCCCCCCCEEEECCCHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCEEE
LMDYDGANSVYASIDNNGWLSFPRFSPDTKSIVYTLTSQSSPSGRVMLYDMRREVSSLLI
EEECCCCCCEEEEECCCCCEECCCCCCCCCEEEEEEECCCCCCCCEEEEHHHHHHHHHHH
GMSGIVSSVRFSPSGREILLSESLNGSTDIYSFSLISKKLTRLTADEFMNTSASYSPDEK
HHHHHHHHEEECCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SIVFASDRDGGSHLYIMDANGSNPRKISSGSGRYMSPVWSPNGTWIAFTKVKGARCYIGI
EEEEEECCCCCCEEEEEECCCCCCCEECCCCCCEECCEECCCCCEEEEEEECCCEEEEEE
MRPNGSKERMIASDCFAEGPSWAPNGQAVLYTKHVQRVEHLATELAMVDLSSMNRRAINT
ECCCCCCCCEEHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PSDALIAHWSNTMQN
CCHHEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA