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The map label for this gene is nuoI

Identifier: 88607532

GI number: 88607532

Start: 844832

End: 845326

Strand: Reverse

Name: nuoI

Synonym: APH_0801

Alternate gene names: 88607532

Gene position: 845326-844832 (Counterclockwise)

Preceding gene: 88607948

Following gene: 88606877

Centisome position: 57.46

GC content: 46.26

Gene sequence:

>495_bases
ATGTTATATTGTGTGCAGTTTGGGAGAAGGGTCATAAGGCTGGTATCTGCAACTGTGCACGGTTTCTTTTTGACGCTTCT
GGCAATGTTTAAGCCCAAAGTTACGTTGCGGTATCCGCATGAGAAGGGGCCTTTGAGTACTAGATTCCGTGGTGAGCATG
CATTGCGTAGGTATGAAAGTGGAGAAGAGAGGTGTATAGCCTGTAAGCTCTGTGAGGCCATTTGTCCAGCTCAAGCTATA
ACGATAGAGGCGGAAGAAAGGAGTGACGGTAGCCGCAGGACTACAAGGTACGACATAGATATGACGAAGTGTATATACTG
CGGATTTTGTCAGGAGGCATGTCCAGTTGATGCTATTGTTGAGGGGCCTAACTTCGAGTACTCTACGGAGACAAGGGAAG
AGTTGATGTATAACAAGGAAAAGCTTCTAGCGAACGGAGACATTTGGGAAGAGGCTTTACGCTATCGGATTAAGAAGAAC
CAGCCTTACTATTAG

Upstream 100 bases:

>100_bases
ATAGGAAAGGCGCTTATAGTTGTTCTTTCTTTTCAGGGTAGTGATGGCTGGTTTCCATTCAAGATTAGGGCGGATAGAAT
CTTACATAAGGTGCGATAAA

Downstream 100 bases:

>100_bases
TTTTCATTTGTGGCTTTGTGGTTTGGGGCGGTATAGCTACTGAGGTAGTTACTGTTCTATATCAGTATGGTGCTAGTTTA
GTTTGGAGTGTTTCCCTATA

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MLYCVQFGRRVIRLVSATVHGFFLTLLAMFKPKVTLRYPHEKGPLSTRFRGEHALRRYESGEERCIACKLCEAICPAQAI
TIEAEERSDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEYSTETREELMYNKEKLLANGDIWEEALRYRIKKN
QPYY

Sequences:

>Translated_164_residues
MLYCVQFGRRVIRLVSATVHGFFLTLLAMFKPKVTLRYPHEKGPLSTRFRGEHALRRYESGEERCIACKLCEAICPAQAI
TIEAEERSDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEYSTETREELMYNKEKLLANGDIWEEALRYRIKKN
QPYY
>Mature_164_residues
MLYCVQFGRRVIRLVSATVHGFFLTLLAMFKPKVTLRYPHEKGPLSTRFRGEHALRRYESGEERCIACKLCEAICPAQAI
TIEAEERSDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEYSTETREELMYNKEKLLANGDIWEEALRYRIKKN
QPYY

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=150, Percent_Identity=74, Blast_Score=231, Evalue=2e-61,
Organism=Escherichia coli, GI1788617, Length=128, Percent_Identity=39.84375, Blast_Score=102, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17555194, Length=138, Percent_Identity=78.9855072463768, Blast_Score=228, Evalue=8e-61,
Organism=Drosophila melanogaster, GI17864306, Length=145, Percent_Identity=77.2413793103448, Blast_Score=238, Evalue=1e-63,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_ANAPZ (Q2GJS3)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505378.1
- HSSP:   Q9I6D2
- ProteinModelPortal:   Q2GJS3
- SMR:   Q2GJS3
- STRING:   Q2GJS3
- GeneID:   3930927
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0801
- NMPDR:   fig|212042.5.peg.766
- TIGR:   APH_0801
- eggNOG:   COG1143
- HOGENOM:   HBG724359
- OMA:   NYPYEKG
- PhylomeDB:   Q2GJS3
- ProtClustDB:   PRK05888
- BioCyc:   APHA212042:APH_0801-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 19128; Mature: 19128

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
5.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYCVQFGRRVIRLVSATVHGFFLTLLAMFKPKVTLRYPHEKGPLSTRFRGEHALRRYES
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHCC
GEERCIACKLCEAICPAQAITIEAEERSDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIV
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEE
EGPNFEYSTETREELMYNKEKLLANGDIWEEALRYRIKKNQPYY
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLYCVQFGRRVIRLVSATVHGFFLTLLAMFKPKVTLRYPHEKGPLSTRFRGEHALRRYES
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHCC
GEERCIACKLCEAICPAQAITIEAEERSDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIV
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEE
EGPNFEYSTETREELMYNKEKLLANGDIWEEALRYRIKKNQPYY
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA