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The map label for this gene is citH [H]

Identifier: 88607308

GI number: 88607308

Start: 898794

End: 900071

Strand: Reverse

Name: citH [H]

Synonym: APH_0848

Alternate gene names: 88607308

Gene position: 900071-898794 (Counterclockwise)

Preceding gene: 88607521

Following gene: 88607787

Centisome position: 61.18

GC content: 43.04

Gene sequence:

>1278_bases
GTGTTTATCAAGACATGTAAGAGAGTTTTTTGCCTTAATGGTGTTGTGCTATCTACCCTTCTATGCAACGCAATAGAGCA
CTACGATTTTATGGTGTACGGTATAATGAGTAGTACCTTGAATAAGGTATTTTTCGTACAAGGTGAGCTTTTTTCCGGCA
CCAATAGCACGTATCTTGCTACGTTGATAGGTTTTTTGGTCTTTGCTGCTGCGTTTGTTTCTAGACCCTTGGGGGCTGTG
TTTTTCGGATATGTAGGTGATAAAAAAGGCCGTAAGGTGGCGCTAACGCTCTCTACCGGTTTGCTGGTAACTTCTGTTTT
AGGAATGGCATTACTCCCTACGCCGAAGGTTTGGGGCATTTTATCTATATTATTTTTGGCTCTTCTCAGAGTGGTTCAGG
GTTTAGCTTTTGGAGCAGAGGTAGGAGGAGTGGTCCTGATGGCTGAGACTGTAGGCGGTAGGAAAGTGCATGCTATTTGG
GTTGCGCGTATTTTCTCCACGACAGTGGGCACGATCTTGGGTGTGTTTGTTGTGCGGATGTGCGAGCTTATTCTATCTCC
TGAGCATATGAATGATTGGGGATGGAGAATACCATTTTTTGCTGCTGTCCTTGTGAGTTTGCCGTTACCGTACTTGCGTA
AGTTTCTCCATGAAAGTGCTGAGTACGTAGAATACAAAGCCAGTGGGCAACGGGAGAATATTTTTAAGTCTTTGTGCTGC
AATATATCCTCTGTTTTGCTCATAATTTCTATGTCGGCGCTTTCATCAGGGTTTTTCTACATGTCTGTGGTGTATATGGA
CATTGTGCACAAGTCTATGTTTTTGGAGCATGAAATGTCCATGCTAGTTTTGGCCTTGGTAGCTTGTAGCAGTTTGGCGG
TATTAGACTTTGCTAAAAGGAAAACTTGTTTTATCTTGATATTGCTTTCGATAGCCTTAACTGCCTATCCGACGGTTTAT
TTTATCGGCAAAGGCTACATGGTGGCACGTGTGCTGTATTTCTCGCTGTTGGGTTTGTATATTGGCTGGTATGGGTCGTT
TATAGCGCTAATTTTTCCGGTAGGAGCTAGGCAGACCTGTTTTTCATTTGCATATAGTTGTGGATATTTGACGGGTGCTC
TGTCGCCAGCGTTATGTTTATGGTTTTCTCACATCACGGGCAAAGACATAATGCCAGCGCTATATATAACATTTCTCTCA
TGTGTGATAGCTTTGATATTTACTTTCGGCGTACGTGTTGAAGGCGATCGGTATCGTCTTACGTTTTCAAAGTGCTAA

Upstream 100 bases:

>100_bases
TTCATGTAAATTATTTAGTATATATGCCGTTATCTGCACTTGTCATGTTTGTGGCGCTGTGTTACTTTTTGTCAAACTCA
TACTAATCGTAGAGGATAGG

Downstream 100 bases:

>100_bases
TTCAGTTGTATCACTGTATATCTCAAAGCTACGCAGTGGCAACGCGATATAACATTCTCTGATGGCGCTGATGTTTGTTT
TTCTATGCACACACATAGAG

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MFIKTCKRVFCLNGVVLSTLLCNAIEHYDFMVYGIMSSTLNKVFFVQGELFSGTNSTYLATLIGFLVFAAAFVSRPLGAV
FFGYVGDKKGRKVALTLSTGLLVTSVLGMALLPTPKVWGILSILFLALLRVVQGLAFGAEVGGVVLMAETVGGRKVHAIW
VARIFSTTVGTILGVFVVRMCELILSPEHMNDWGWRIPFFAAVLVSLPLPYLRKFLHESAEYVEYKASGQRENIFKSLCC
NISSVLLIISMSALSSGFFYMSVVYMDIVHKSMFLEHEMSMLVLALVACSSLAVLDFAKRKTCFILILLSIALTAYPTVY
FIGKGYMVARVLYFSLLGLYIGWYGSFIALIFPVGARQTCFSFAYSCGYLTGALSPALCLWFSHITGKDIMPALYITFLS
CVIALIFTFGVRVEGDRYRLTFSKC

Sequences:

>Translated_425_residues
MFIKTCKRVFCLNGVVLSTLLCNAIEHYDFMVYGIMSSTLNKVFFVQGELFSGTNSTYLATLIGFLVFAAAFVSRPLGAV
FFGYVGDKKGRKVALTLSTGLLVTSVLGMALLPTPKVWGILSILFLALLRVVQGLAFGAEVGGVVLMAETVGGRKVHAIW
VARIFSTTVGTILGVFVVRMCELILSPEHMNDWGWRIPFFAAVLVSLPLPYLRKFLHESAEYVEYKASGQRENIFKSLCC
NISSVLLIISMSALSSGFFYMSVVYMDIVHKSMFLEHEMSMLVLALVACSSLAVLDFAKRKTCFILILLSIALTAYPTVY
FIGKGYMVARVLYFSLLGLYIGWYGSFIALIFPVGARQTCFSFAYSCGYLTGALSPALCLWFSHITGKDIMPALYITFLS
CVIALIFTFGVRVEGDRYRLTFSKC
>Mature_425_residues
MFIKTCKRVFCLNGVVLSTLLCNAIEHYDFMVYGIMSSTLNKVFFVQGELFSGTNSTYLATLIGFLVFAAAFVSRPLGAV
FFGYVGDKKGRKVALTLSTGLLVTSVLGMALLPTPKVWGILSILFLALLRVVQGLAFGAEVGGVVLMAETVGGRKVHAIW
VARIFSTTVGTILGVFVVRMCELILSPEHMNDWGWRIPFFAAVLVSLPLPYLRKFLHESAEYVEYKASGQRENIFKSLCC
NISSVLLIISMSALSSGFFYMSVVYMDIVHKSMFLEHEMSMLVLALVACSSLAVLDFAKRKTCFILILLSIALTAYPTVY
FIGKGYMVARVLYFSLLGLYIGWYGSFIALIFPVGARQTCFSFAYSCGYLTGALSPALCLWFSHITGKDIMPALYITFLS
CVIALIFTFGVRVEGDRYRLTFSKC

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=418, Percent_Identity=27.7511961722488, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1788292, Length=233, Percent_Identity=29.6137339055794, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1789941, Length=433, Percent_Identity=29.7921478060046, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788942, Length=267, Percent_Identity=30.3370786516854, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46964; Mature: 46964

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIKTCKRVFCLNGVVLSTLLCNAIEHYDFMVYGIMSSTLNKVFFVQGELFSGTNSTYLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECEEECCCCCHHHH
TLIGFLVFAAAFVSRPLGAVFFGYVGDKKGRKVALTLSTGLLVTSVLGMALLPTPKVWGI
HHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCHHHHH
LSILFLALLRVVQGLAFGAEVGGVVLMAETVGGRKVHAIWVARIFSTTVGTILGVFVVRM
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
CELILSPEHMNDWGWRIPFFAAVLVSLPLPYLRKFLHESAEYVEYKASGQRENIFKSLCC
HHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NISSVLLIISMSALSSGFFYMSVVYMDIVHKSMFLEHEMSMLVLALVACSSLAVLDFAKR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTCFILILLSIALTAYPTVYFIGKGYMVARVLYFSLLGLYIGWYGSFIALIFPVGARQTC
HHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
FSFAYSCGYLTGALSPALCLWFSHITGKDIMPALYITFLSCVIALIFTFGVRVEGDRYRL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCEEEE
TFSKC
EEECC
>Mature Secondary Structure
MFIKTCKRVFCLNGVVLSTLLCNAIEHYDFMVYGIMSSTLNKVFFVQGELFSGTNSTYLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECEEECCCCCHHHH
TLIGFLVFAAAFVSRPLGAVFFGYVGDKKGRKVALTLSTGLLVTSVLGMALLPTPKVWGI
HHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCHHHHH
LSILFLALLRVVQGLAFGAEVGGVVLMAETVGGRKVHAIWVARIFSTTVGTILGVFVVRM
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
CELILSPEHMNDWGWRIPFFAAVLVSLPLPYLRKFLHESAEYVEYKASGQRENIFKSLCC
HHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NISSVLLIISMSALSSGFFYMSVVYMDIVHKSMFLEHEMSMLVLALVACSSLAVLDFAKR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTCFILILLSIALTAYPTVYFIGKGYMVARVLYFSLLGLYIGWYGSFIALIFPVGARQTC
HHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
FSFAYSCGYLTGALSPALCLWFSHITGKDIMPALYITFLSCVIALIFTFGVRVEGDRYRL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCEEEE
TFSKC
EEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]