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The map label for this gene is hemB [H]

Identifier: 88607137

GI number: 88607137

Start: 903572

End: 904564

Strand: Direct

Name: hemB [H]

Synonym: APH_0851

Alternate gene names: 88607137

Gene position: 903572-904564 (Clockwise)

Preceding gene: 88607851

Following gene: 88606869

Centisome position: 61.41

GC content: 47.43

Gene sequence:

>993_bases
ATGCAGTTCCCAAAAACTCGACTTAGACGTAAGCGCGCTCACGGGTGGATGCGTGCCATGGTTAGGGAGACGGTGTTACA
TCCCAAGGATCTGATCTTGCCTATATTTATCCATGAGAAAGATGAAGCGTCTGTAGTATTAGAAGGGTTGGACTGCATGA
GGCGAGTCTCAATGACGGAAGCGGCAAATCTCGCTCAGGAGGCTCACAGCTTAGGCATTAGTGCTGTCATACTATTTCCT
GCAGACTGTGAGAAGTCTCCTGATGCTGCTGAGGCTTGTAATCCAGAAAATTTAGTTTGCAGAACAATAAGACTCATCAA
GGAATCTGTTCCTGATATAGGCGTGATGGCAGATGTTGCTTTAGATCCGTACACGACCTCTGGGCACGATGGGATTGTCG
TAAATGAGGTAGTGGATAATGATGCCACGTTGGATGTATTGTGTAAGCAAGCGGTTGTCCTTGCTGCAGCTGGGTGTGAT
GTAGTAGCCCCTTCTGAAATGATGGATGGTAGGGTTGGAGTCATACGTGAATTCCTTGATAATTGTTCATTTAGTCACGT
AAGCATTTTGTCTTATGCCGCAAAGTTTTGCTCACACCTCCATAGGCCATTCCGCGTAGTGGTTGGGTCGCGTACAGCAT
CTCAATCTGTGGATAAAAGCACATACCATATGGATCCTGCAAATGCCCGAGAGGCGCTGCTAGAAGCACAACTTGATATT
GATGAAGGGGCTGACATGATACTGGTCAAGCCAGGAATTTTTTACTTAGATGTCCTTTCTTCCATAAGCAACACGTTTGA
TATACCTGTGCTTACTTATCAGGTTAGTGGGGAGTATGCCATGCTGAAGGCCGCGGCCCTGCAGGGATGGTTGGATTATG
AACAATGTATGCACGAGGCACTGCTGAGTATGAAGCGTGCTGGCGCACGAAATATTATTACCTATGCCGCGCTGGAAGTT
GCAAGGAAGTTACGCGGCGCGGGTGATGTGTAG

Upstream 100 bases:

>100_bases
AAAATTACTAACTATCTTAATTTCTAATAACCCAATACAAATGGATTGATGTCATCTTACAAAAAAAATATACTCAGAGC
AGGTATTTTGTGTTTTGAGT

Downstream 100 bases:

>100_bases
TGACAAGAATACTCGTAAAGCAGCTAAATTATGGCCCTAATGCAGTTCATAGTAACTGTGAGAGGATACTTGCGGAATGT
AGAGAGGCATCGGCCGGAGA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MQFPKTRLRRKRAHGWMRAMVRETVLHPKDLILPIFIHEKDEASVVLEGLDCMRRVSMTEAANLAQEAHSLGISAVILFP
ADCEKSPDAAEACNPENLVCRTIRLIKESVPDIGVMADVALDPYTTSGHDGIVVNEVVDNDATLDVLCKQAVVLAAAGCD
VVAPSEMMDGRVGVIREFLDNCSFSHVSILSYAAKFCSHLHRPFRVVVGSRTASQSVDKSTYHMDPANAREALLEAQLDI
DEGADMILVKPGIFYLDVLSSISNTFDIPVLTYQVSGEYAMLKAAALQGWLDYEQCMHEALLSMKRAGARNIITYAALEV
ARKLRGAGDV

Sequences:

>Translated_330_residues
MQFPKTRLRRKRAHGWMRAMVRETVLHPKDLILPIFIHEKDEASVVLEGLDCMRRVSMTEAANLAQEAHSLGISAVILFP
ADCEKSPDAAEACNPENLVCRTIRLIKESVPDIGVMADVALDPYTTSGHDGIVVNEVVDNDATLDVLCKQAVVLAAAGCD
VVAPSEMMDGRVGVIREFLDNCSFSHVSILSYAAKFCSHLHRPFRVVVGSRTASQSVDKSTYHMDPANAREALLEAQLDI
DEGADMILVKPGIFYLDVLSSISNTFDIPVLTYQVSGEYAMLKAAALQGWLDYEQCMHEALLSMKRAGARNIITYAALEV
ARKLRGAGDV
>Mature_330_residues
MQFPKTRLRRKRAHGWMRAMVRETVLHPKDLILPIFIHEKDEASVVLEGLDCMRRVSMTEAANLAQEAHSLGISAVILFP
ADCEKSPDAAEACNPENLVCRTIRLIKESVPDIGVMADVALDPYTTSGHDGIVVNEVVDNDATLDVLCKQAVVLAAAGCD
VVAPSEMMDGRVGVIREFLDNCSFSHVSILSYAAKFCSHLHRPFRVVVGSRTASQSVDKSTYHMDPANAREALLEAQLDI
DEGADMILVKPGIFYLDVLSSISNTFDIPVLTYQVSGEYAMLKAAALQGWLDYEQCMHEALLSMKRAGARNIITYAALEV
ARKLRGAGDV

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=328, Percent_Identity=33.5365853658537, Blast_Score=164, Evalue=9e-41,
Organism=Escherichia coli, GI87081728, Length=317, Percent_Identity=41.6403785488959, Blast_Score=224, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6321398, Length=312, Percent_Identity=35.5769230769231, Blast_Score=172, Evalue=9e-44,
Organism=Drosophila melanogaster, GI21358291, Length=317, Percent_Identity=33.7539432176656, Blast_Score=167, Evalue=9e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36176; Mature: 36176

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFPKTRLRRKRAHGWMRAMVRETVLHPKDLILPIFIHEKDEASVVLEGLDCMRRVSMTE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHH
AANLAQEAHSLGISAVILFPADCEKSPDAAEACNPENLVCRTIRLIKESVPDIGVMADVA
HHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCE
LDPYTTSGHDGIVVNEVVDNDATLDVLCKQAVVLAAAGCDVVAPSEMMDGRVGVIREFLD
ECCCCCCCCCCEEEEEHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHH
NCSFSHVSILSYAAKFCSHLHRPFRVVVGSRTASQSVDKSTYHMDPANAREALLEAQLDI
CCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCC
DEGADMILVKPGIFYLDVLSSISNTFDIPVLTYQVSGEYAMLKAAALQGWLDYEQCMHEA
CCCCCEEEECCCHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
LLSMKRAGARNIITYAALEVARKLRGAGDV
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQFPKTRLRRKRAHGWMRAMVRETVLHPKDLILPIFIHEKDEASVVLEGLDCMRRVSMTE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHH
AANLAQEAHSLGISAVILFPADCEKSPDAAEACNPENLVCRTIRLIKESVPDIGVMADVA
HHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCE
LDPYTTSGHDGIVVNEVVDNDATLDVLCKQAVVLAAAGCDVVAPSEMMDGRVGVIREFLD
ECCCCCCCCCCEEEEEHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHH
NCSFSHVSILSYAAKFCSHLHRPFRVVVGSRTASQSVDKSTYHMDPANAREALLEAQLDI
CCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCC
DEGADMILVKPGIFYLDVLSSISNTFDIPVLTYQVSGEYAMLKAAALQGWLDYEQCMHEA
CCCCCEEEECCCHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
LLSMKRAGARNIITYAALEVARKLRGAGDV
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]