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The map label for this gene is nadE [H]

Identifier: 88606869

GI number: 88606869

Start: 904564

End: 906366

Strand: Direct

Name: nadE [H]

Synonym: APH_0852

Alternate gene names: 88606869

Gene position: 904564-906366 (Clockwise)

Preceding gene: 88607137

Following gene: 88607706

Centisome position: 61.48

GC content: 42.32

Gene sequence:

>1803_bases
GTGACAAGAATACTCGTAAAGCAGCTAAATTATGGCCCTAATGCAGTTCATAGTAACTGTGAGAGGATACTTGCGGAATG
TAGAGAGGCATCGGCCGGAGATATTGTGCTATTTAGCAGGTATGCAGTTTGCGGATACTTTGACAAGGCTCCCCTGCTTT
CTGGAAACCTTTTGCAGCTTTGTAACAAACACCTGGAGAGCTTAGCTTCAAGTATAGGTAGCGCTTGTGCTATTATAGGA
GGGCTGGCGCTGCAAGATGGGGTGTTGACTGAAGCTATATATCTCATATCAGACGGTAACTACGAGAAACTTATAGAGCT
TCCTATTTCTTCGTACAATGCAGCAGAGAAACATATTGTTTTAAGCTTAGGTGGCTTGCGTATAGCATTGCTATTGGAAG
AACATACTGCACATTCAAATGCGCAAACCTGGCATGGTGCATATCAAAATGTGCCCACCGATATAGATGTACTAATACTA
TTAGGAAGGTCACAGTATAGCTACTACAGTTTGCTTAGTTCCAATGCTGCAATTTCTATAATGCAGAACCATCGAGCATT
TATTTATCTTAACATACTGGGAGGGTATGGCAGCGATGTCTTTGCAGGGGGGTCTTTAATACACGATGGAATAAATGCCA
CTACACTGGCACTTTGTAAGGAAGACGCTAGGATTATTGAAATTACAGCTACGAACAGCATAGTAACGTGCGCTGATGGA
GTTCCTAGAGCGCATTCAGAACATATTTCGGATCACAATTGTACTTCCGGTATCTCTGCGTCGGAGAATCTTACCCTTGA
TGCATCTCCTACTTCAGAGAGAAGTGTAACTGAAGGGGTTTTATCCACTTCAAAACACGTTGAGCAGAGCCCTAACATTG
GAGTATTGGAATCTCATACTCTAAATGCGCACTCCCAATTGCATAGTCTCAAAACCGATAAAGTTTTTAGTCTTGCTGAG
CTAAAGTCCCTGAACATGGACTGGGATGTGTTTACGTATCAAATGCTGGTGCTAGCATTGAGGGATTATGTAAAAAAAAG
CGGTTTCTCCGGTGTGCTATTGGGGTTATCTGGCGGTATAGACTCCGCGCTCGTCGCTGCTATAGCTTCTGACGCTCTAG
GGGCAGAGCATGTGCATACATTTATGTTGACCACAAGACATACCTCTCAGTCCAGCGTAACCGACGCACAAAGGTGCGCA
GAACTTCTTGGAACGCATCATGAAGTTGTATCGATAGAAGAGGCGTTCTGCACTTGTATTGAGAGTTTAAAGACATATAT
TGATACTCCTACTCCTAATAATGCACTAGAAAACATGCAGTCTAGAATTAGGGGGATGTATTTGATGGCCATCAGCAATG
CTAACTCGCTTCTTCTTCTAGCTACGGGAAATAAGTCGGAATTGCTGACAGGCTATATGACGTTATACGGGGATACTTGC
GGGGGATATGCTCCGATTAACAATGTATATAAGACAAAGGTTTATGATCTTGTGAAGTGGCGTAACAGCAATATTCCCGC
AAATTCACTTTGTAGAAAAATGCATGTAATTCCCGAAAATATAATAACAAAGGCCCCTTCAGCAGAGTTAAAACCTAATC
AGACTGATCAAGATACTTTGCCTGAGTACGATAAACTTGATGGAATTCTAAGTTTATTAGTAGACAGATTTGCTACTAGG
GAAGATATCGTGCTTTCTGGTTACACTGAAGAAGAAGTGGATCTGGTTATGAATCTTGTGAAAAAATCTGCTTTCAAGCT
CGATCAGGTTGCACCAGGACCGGACATACGTGCCAAATCATAA

Upstream 100 bases:

>100_bases
ACGAGGCACTGCTGAGTATGAAGCGTGCTGGCGCACGAAATATTATTACCTATGCCGCGCTGGAAGTTGCAAGGAAGTTA
CGCGGCGCGGGTGATGTGTA

Downstream 100 bases:

>100_bases
AAAACAAAACTACTACTGTGTTGGCATTTTTTTATTCCACAATCTCGGGATGTTGGCTGAACAGCATAAAATTGCGTGGA
CATGCGGTTGATCTCAGAAA

Product: putative glutamine-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 600; Mature: 599

Protein sequence:

>600_residues
MTRILVKQLNYGPNAVHSNCERILAECREASAGDIVLFSRYAVCGYFDKAPLLSGNLLQLCNKHLESLASSIGSACAIIG
GLALQDGVLTEAIYLISDGNYEKLIELPISSYNAAEKHIVLSLGGLRIALLLEEHTAHSNAQTWHGAYQNVPTDIDVLIL
LGRSQYSYYSLLSSNAAISIMQNHRAFIYLNILGGYGSDVFAGGSLIHDGINATTLALCKEDARIIEITATNSIVTCADG
VPRAHSEHISDHNCTSGISASENLTLDASPTSERSVTEGVLSTSKHVEQSPNIGVLESHTLNAHSQLHSLKTDKVFSLAE
LKSLNMDWDVFTYQMLVLALRDYVKKSGFSGVLLGLSGGIDSALVAAIASDALGAEHVHTFMLTTRHTSQSSVTDAQRCA
ELLGTHHEVVSIEEAFCTCIESLKTYIDTPTPNNALENMQSRIRGMYLMAISNANSLLLLATGNKSELLTGYMTLYGDTC
GGYAPINNVYKTKVYDLVKWRNSNIPANSLCRKMHVIPENIITKAPSAELKPNQTDQDTLPEYDKLDGILSLLVDRFATR
EDIVLSGYTEEEVDLVMNLVKKSAFKLDQVAPGPDIRAKS

Sequences:

>Translated_600_residues
MTRILVKQLNYGPNAVHSNCERILAECREASAGDIVLFSRYAVCGYFDKAPLLSGNLLQLCNKHLESLASSIGSACAIIG
GLALQDGVLTEAIYLISDGNYEKLIELPISSYNAAEKHIVLSLGGLRIALLLEEHTAHSNAQTWHGAYQNVPTDIDVLIL
LGRSQYSYYSLLSSNAAISIMQNHRAFIYLNILGGYGSDVFAGGSLIHDGINATTLALCKEDARIIEITATNSIVTCADG
VPRAHSEHISDHNCTSGISASENLTLDASPTSERSVTEGVLSTSKHVEQSPNIGVLESHTLNAHSQLHSLKTDKVFSLAE
LKSLNMDWDVFTYQMLVLALRDYVKKSGFSGVLLGLSGGIDSALVAAIASDALGAEHVHTFMLTTRHTSQSSVTDAQRCA
ELLGTHHEVVSIEEAFCTCIESLKTYIDTPTPNNALENMQSRIRGMYLMAISNANSLLLLATGNKSELLTGYMTLYGDTC
GGYAPINNVYKTKVYDLVKWRNSNIPANSLCRKMHVIPENIITKAPSAELKPNQTDQDTLPEYDKLDGILSLLVDRFATR
EDIVLSGYTEEEVDLVMNLVKKSAFKLDQVAPGPDIRAKS
>Mature_599_residues
TRILVKQLNYGPNAVHSNCERILAECREASAGDIVLFSRYAVCGYFDKAPLLSGNLLQLCNKHLESLASSIGSACAIIGG
LALQDGVLTEAIYLISDGNYEKLIELPISSYNAAEKHIVLSLGGLRIALLLEEHTAHSNAQTWHGAYQNVPTDIDVLILL
GRSQYSYYSLLSSNAAISIMQNHRAFIYLNILGGYGSDVFAGGSLIHDGINATTLALCKEDARIIEITATNSIVTCADGV
PRAHSEHISDHNCTSGISASENLTLDASPTSERSVTEGVLSTSKHVEQSPNIGVLESHTLNAHSQLHSLKTDKVFSLAEL
KSLNMDWDVFTYQMLVLALRDYVKKSGFSGVLLGLSGGIDSALVAAIASDALGAEHVHTFMLTTRHTSQSSVTDAQRCAE
LLGTHHEVVSIEEAFCTCIESLKTYIDTPTPNNALENMQSRIRGMYLMAISNANSLLLLATGNKSELLTGYMTLYGDTCG
GYAPINNVYKTKVYDLVKWRNSNIPANSLCRKMHVIPENIITKAPSAELKPNQTDQDTLPEYDKLDGILSLLVDRFATRE
DIVLSGYTEEEVDLVMNLVKKSAFKLDQVAPGPDIRAKS

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 65160; Mature: 65029

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRILVKQLNYGPNAVHSNCERILAECREASAGDIVLFSRYAVCGYFDKAPLLSGNLLQL
CCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECHHHHCCCCCCCCCCCHHHHH
CNKHLESLASSIGSACAIIGGLALQDGVLTEAIYLISDGNYEKLIELPISSYNAAEKHIV
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEEECCCCEEEEECCCCCCCCCCCEEE
LSLGGLRIALLLEEHTAHSNAQTWHGAYQNVPTDIDVLILLGRSQYSYYSLLSSNAAISI
EEECCEEEEEEEEHHCCCCCCCHHCCHHCCCCCCCEEEEEECCCCCHHHHHHHCCCEEEE
MQNHRAFIYLNILGGYGSDVFAGGSLIHDGINATTLALCKEDARIIEITATNSIVTCADG
EECCCEEEEEEEECCCCCCHHCCCHHHCCCCCHHHHEEECCCCEEEEEEECCCEEEECCC
VPRAHSEHISDHNCTSGISASENLTLDASPTSERSVTEGVLSTSKHVEQSPNIGVLESHT
CCCCHHHCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCC
LNAHSQLHSLKTDKVFSLAELKSLNMDWDVFTYQMLVLALRDYVKKSGFSGVLLGLSGGI
CCHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCH
DSALVAAIASDALGAEHVHTFMLTTRHTSQSSVTDAQRCAELLGTHHEVVSIEEAFCTCI
HHHHHHHHHHHCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ESLKTYIDTPTPNNALENMQSRIRGMYLMAISNANSLLLLATGNKSELLTGYMTLYGDTC
HHHHHHCCCCCCCHHHHHHHHHHCEEEEEEEECCCEEEEEEECCCCHHHHHHHHEECCCC
GGYAPINNVYKTKVYDLVKWRNSNIPANSLCRKMHVIPENIITKAPSAELKPNQTDQDTL
CCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCC
PEYDKLDGILSLLVDRFATREDIVLSGYTEEEVDLVMNLVKKSAFKLDQVAPGPDIRAKS
CCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TRILVKQLNYGPNAVHSNCERILAECREASAGDIVLFSRYAVCGYFDKAPLLSGNLLQL
CCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECHHHHCCCCCCCCCCCHHHHH
CNKHLESLASSIGSACAIIGGLALQDGVLTEAIYLISDGNYEKLIELPISSYNAAEKHIV
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEEECCCCEEEEECCCCCCCCCCCEEE
LSLGGLRIALLLEEHTAHSNAQTWHGAYQNVPTDIDVLILLGRSQYSYYSLLSSNAAISI
EEECCEEEEEEEEHHCCCCCCCHHCCHHCCCCCCCEEEEEECCCCCHHHHHHHCCCEEEE
MQNHRAFIYLNILGGYGSDVFAGGSLIHDGINATTLALCKEDARIIEITATNSIVTCADG
EECCCEEEEEEEECCCCCCHHCCCHHHCCCCCHHHHEEECCCCEEEEEEECCCEEEECCC
VPRAHSEHISDHNCTSGISASENLTLDASPTSERSVTEGVLSTSKHVEQSPNIGVLESHT
CCCCHHHCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCC
LNAHSQLHSLKTDKVFSLAELKSLNMDWDVFTYQMLVLALRDYVKKSGFSGVLLGLSGGI
CCHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCH
DSALVAAIASDALGAEHVHTFMLTTRHTSQSSVTDAQRCAELLGTHHEVVSIEEAFCTCI
HHHHHHHHHHHCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ESLKTYIDTPTPNNALENMQSRIRGMYLMAISNANSLLLLATGNKSELLTGYMTLYGDTC
HHHHHHCCCCCCCHHHHHHHHHHCEEEEEEEECCCEEEEEEECCCCHHHHHHHHEECCCC
GGYAPINNVYKTKVYDLVKWRNSNIPANSLCRKMHVIPENIITKAPSAELKPNQTDQDTL
CCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCC
PEYDKLDGILSLLVDRFATREDIVLSGYTEEEVDLVMNLVKKSAFKLDQVAPGPDIRAKS
CCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]