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The map label for this gene is purF [H]

Identifier: 88607063

GI number: 88607063

Start: 1367224

End: 1368630

Strand: Reverse

Name: purF [H]

Synonym: APH_1300

Alternate gene names: 88607063

Gene position: 1368630-1367224 (Counterclockwise)

Preceding gene: 88607642

Following gene: 88607244

Centisome position: 93.02

GC content: 45.06

Gene sequence:

>1407_bases
ATGCCGTTTGCCAGTATATATGAGGAATGCGGAATATTTGCCGTGCAAAATCACAGCAATGCTGTGCACAAGTGCCTGTT
GGGTTTGCACGCACTGCAGCATCGTGGTCAGGAGTCATTTGGTATTGCAGCGTCAACACCGGGCATTTCGGAGCTGGTTT
CGTTTTATAGTGGAGGCTGCGTTAGCAACATATTTTCTAACCCCGAGGTTCATACTCTTACCGGAAGGATGGCGATTGGA
CATGTAAGATATTCCACAAGTGGGGGTCTAACAGCTTCAGGAGCGCAGCCTTTTATTGTGCAAGGGAGGTTCGGGCCGCT
TGCCGTGGCACACAATGGCAATTTGACAAATGCCCGAAGTTTGAGGGAAGAACTCATAGACAAGGGCTGTCAATTTACCT
CCGAAATAGATACAGAGGTTGTTGCTCATTTAGCGGTAGTAGACGAAGCAGAAACTTTTGTGGATTGTGTGATCAATGCA
CTAAAAAGGACAAAGGGAGCCTACTCGTTTGTTATAATGGTACGTGACACACTTATAGGCGCGCGAGATCCTGCGGGGAT
CAGACCGTTGGTATTAGGAATGGTAGAAGGTTCGTACGTCTTAGCTTCGGAGACCTGTGCTTTAGACATTGTAGGAGCAA
CATTTGTACGTGATATTAAGCCAGGTGAACTAGTAGTTATAGATAAGTCAAATAATATAACTAGTTTGTTTCCGTTTAAG
CCTCTGAAGTATAGTTTCTGCATTTTTGAGTACGTATATTTTGCGCGTCCTGATAGTGTGGTAGAGAGCAAACCTGTGTA
TGAAACACGTAAAAACATTGGTGCGATGCTTGCTACAGAAAGCCCTCCTCCAAGTGATGTAGACATGGTAGTGCCGGTAC
CGGATTCTGGCATTCCGGCGGCTATGGGTTATGCAACAAAATCAGGAGTGCCATTTGAGTTCGGCATAGTAAGGAACCGT
TACGTAGGGCGCACGTTCATACAGCCAACGGATAAAGCAAGGAAGCTTGGTGTTGAGCTAAAACACAATGCAAACTCGGC
AATTTTAAAAGGGAAGAGCATAGTATTGGTGGATGACAGCATAGTACGTGGCACAACACTGCGTGAAGTTATAGTTTTAC
TTAGAAAAGCCGGTACCAAAAATATACATCTTAGGATATCGAGCCCTCCTACAGTGTACTCATGTTTCTATGGGATAGAT
ACTCCACATAGGTCAAGTTTGGTAGCTAATAACATGTCTATAGCAGATCTAGTGAAGATGCTAGATTGCGACAGTCTCTC
ATTCATCAGCATAGACGGGCTATATAAAGCCATAAGGGGCGCAAAGCGAGACAGTGACAATCCTCAGTACTGTGATGCAT
GCTTCACCGGGTATTACCCCGTGGGTGAAATGGAGTGTAATAGCTAA

Upstream 100 bases:

>100_bases
CGAGTATTGTTATGCACACTTGAGGCCAATAATGCAATTATTACTAGAGGGAACTATTCCTAAATGTTAAAATGCTTAAT
GGTAGAGTTTGGGGAGGCGC

Downstream 100 bases:

>100_bases
TCATACCGTACAGGAGCGATTGTTCGTGCATGTTCTACTGTAGGTGCGGTTTTAATAAACAGGCTGGATACATGGGTATT
GCGCTTGTGTTTAATTAGAT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MPFASIYEECGIFAVQNHSNAVHKCLLGLHALQHRGQESFGIAASTPGISELVSFYSGGCVSNIFSNPEVHTLTGRMAIG
HVRYSTSGGLTASGAQPFIVQGRFGPLAVAHNGNLTNARSLREELIDKGCQFTSEIDTEVVAHLAVVDEAETFVDCVINA
LKRTKGAYSFVIMVRDTLIGARDPAGIRPLVLGMVEGSYVLASETCALDIVGATFVRDIKPGELVVIDKSNNITSLFPFK
PLKYSFCIFEYVYFARPDSVVESKPVYETRKNIGAMLATESPPPSDVDMVVPVPDSGIPAAMGYATKSGVPFEFGIVRNR
YVGRTFIQPTDKARKLGVELKHNANSAILKGKSIVLVDDSIVRGTTLREVIVLLRKAGTKNIHLRISSPPTVYSCFYGID
TPHRSSLVANNMSIADLVKMLDCDSLSFISIDGLYKAIRGAKRDSDNPQYCDACFTGYYPVGEMECNS

Sequences:

>Translated_468_residues
MPFASIYEECGIFAVQNHSNAVHKCLLGLHALQHRGQESFGIAASTPGISELVSFYSGGCVSNIFSNPEVHTLTGRMAIG
HVRYSTSGGLTASGAQPFIVQGRFGPLAVAHNGNLTNARSLREELIDKGCQFTSEIDTEVVAHLAVVDEAETFVDCVINA
LKRTKGAYSFVIMVRDTLIGARDPAGIRPLVLGMVEGSYVLASETCALDIVGATFVRDIKPGELVVIDKSNNITSLFPFK
PLKYSFCIFEYVYFARPDSVVESKPVYETRKNIGAMLATESPPPSDVDMVVPVPDSGIPAAMGYATKSGVPFEFGIVRNR
YVGRTFIQPTDKARKLGVELKHNANSAILKGKSIVLVDDSIVRGTTLREVIVLLRKAGTKNIHLRISSPPTVYSCFYGID
TPHRSSLVANNMSIADLVKMLDCDSLSFISIDGLYKAIRGAKRDSDNPQYCDACFTGYYPVGEMECNS
>Mature_467_residues
PFASIYEECGIFAVQNHSNAVHKCLLGLHALQHRGQESFGIAASTPGISELVSFYSGGCVSNIFSNPEVHTLTGRMAIGH
VRYSTSGGLTASGAQPFIVQGRFGPLAVAHNGNLTNARSLREELIDKGCQFTSEIDTEVVAHLAVVDEAETFVDCVINAL
KRTKGAYSFVIMVRDTLIGARDPAGIRPLVLGMVEGSYVLASETCALDIVGATFVRDIKPGELVVIDKSNNITSLFPFKP
LKYSFCIFEYVYFARPDSVVESKPVYETRKNIGAMLATESPPPSDVDMVVPVPDSGIPAAMGYATKSGVPFEFGIVRNRY
VGRTFIQPTDKARKLGVELKHNANSAILKGKSIVLVDDSIVRGTTLREVIVLLRKAGTKNIHLRISSPPTVYSCFYGIDT
PHRSSLVANNMSIADLVKMLDCDSLSFISIDGLYKAIRGAKRDSDNPQYCDACFTGYYPVGEMECNS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=515, Percent_Identity=37.8640776699029, Blast_Score=327, Evalue=1e-89,
Organism=Escherichia coli, GI1788651, Length=470, Percent_Identity=35.7446808510638, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1790167, Length=212, Percent_Identity=33.4905660377358, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17554892, Length=480, Percent_Identity=36.875, Blast_Score=300, Evalue=9e-82,
Organism=Saccharomyces cerevisiae, GI6323958, Length=480, Percent_Identity=35, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24659598, Length=487, Percent_Identity=40.4517453798768, Blast_Score=345, Evalue=4e-95,
Organism=Drosophila melanogaster, GI28573187, Length=495, Percent_Identity=39.1919191919192, Blast_Score=344, Evalue=6e-95,
Organism=Drosophila melanogaster, GI24659604, Length=463, Percent_Identity=41.4686825053996, Blast_Score=342, Evalue=3e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 50747; Mature: 50616

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFASIYEECGIFAVQNHSNAVHKCLLGLHALQHRGQESFGIAASTPGISELVSFYSGGC
CCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHCCCH
VSNIFSNPEVHTLTGRMAIGHVRYSTSGGLTASGAQPFIVQGRFGPLAVAHNGNLTNARS
HHHHCCCCCEEEEECCEEEEEEEEECCCCEEECCCCCEEEECCCCCEEEEECCCCCHHHH
LREELIDKGCQFTSEIDTEVVAHLAVVDEAETFVDCVINALKRTKGAYSFVIMVRDTLIG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHCC
ARDPAGIRPLVLGMVEGSYVLASETCALDIVGATFVRDIKPGELVVIDKSNNITSLFPFK
CCCCCCCCEEEEEEECCCEEEEECCEEEEEECHHHHHCCCCCEEEEEECCCCEEEECCCC
PLKYSFCIFEYVYFARPDSVVESKPVYETRKNIGAMLATESPPPSDVDMVVPVPDSGIPA
CCHHHHHHHHHHHHCCCCHHHCCCCHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCCH
AMGYATKSGVPFEFGIVRNRYVGRTFIQPTDKARKLGVELKHNANSAILKGKSIVLVDDS
HHCCCCCCCCCEEEEEHHCCCCCCEECCCCHHHHHCCCEEECCCCCEEEECCEEEEECCC
IVRGTTLREVIVLLRKAGTKNIHLRISSPPTVYSCFYGIDTPHRSSLVANNMSIADLVKM
CCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHCCCCCHHHHHHH
LDCDSLSFISIDGLYKAIRGAKRDSDNPQYCDACFTGYYPVGEMECNS
HCCCCCCEEEHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
PFASIYEECGIFAVQNHSNAVHKCLLGLHALQHRGQESFGIAASTPGISELVSFYSGGC
CHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHCCCH
VSNIFSNPEVHTLTGRMAIGHVRYSTSGGLTASGAQPFIVQGRFGPLAVAHNGNLTNARS
HHHHCCCCCEEEEECCEEEEEEEEECCCCEEECCCCCEEEECCCCCEEEEECCCCCHHHH
LREELIDKGCQFTSEIDTEVVAHLAVVDEAETFVDCVINALKRTKGAYSFVIMVRDTLIG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHCC
ARDPAGIRPLVLGMVEGSYVLASETCALDIVGATFVRDIKPGELVVIDKSNNITSLFPFK
CCCCCCCCEEEEEEECCCEEEEECCEEEEEECHHHHHCCCCCEEEEEECCCCEEEECCCC
PLKYSFCIFEYVYFARPDSVVESKPVYETRKNIGAMLATESPPPSDVDMVVPVPDSGIPA
CCHHHHHHHHHHHHCCCCHHHCCCCHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCCH
AMGYATKSGVPFEFGIVRNRYVGRTFIQPTDKARKLGVELKHNANSAILKGKSIVLVDDS
HHCCCCCCCCCEEEEEHHCCCCCCEECCCCHHHHHCCCEEECCCCCEEEECCEEEEECCC
IVRGTTLREVIVLLRKAGTKNIHLRISSPPTVYSCFYGIDTPHRSSLVANNMSIADLVKM
CCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHCCCCCHHHHHHH
LDCDSLSFISIDGLYKAIRGAKRDSDNPQYCDACFTGYYPVGEMECNS
HCCCCCCEEEHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]