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The map label for this gene is ywkE [H]

Identifier: 88196021

GI number: 88196021

Start: 2179756

End: 2180508

Strand: Reverse

Name: ywkE [H]

Synonym: SAOUHSC_02358

Alternate gene names: 88196021

Gene position: 2180508-2179756 (Counterclockwise)

Preceding gene: 88196022

Following gene: 88196020

Centisome position: 77.29

GC content: 34.93

Gene sequence:

>753_bases
ATGTTAGATGTATTTCAATGGACGCGTACGGACTTTGTAGTCCACATGCATGATGATATGCCGAAAGCGATGATTATGAA
GTTCGACTTAGCATTACAACGTATGTTATTAGGAGAGCCTATACAGTATATAGTTGGCTTTGCCTCATTTTATGGTAGAA
CGTTTGATGTAAACTCAAATTGTTTGATACCAAGACCTGAAACTGAAGAAGTAATGTTGCATTTCTTACAACAGTTAGAA
GATGATGCAACAATCGTAGATATCGGAACGGGTAGTGGTGTACTTGCAATTACTTTGAAATGTGAAAAGCCGGATTTAAA
TGTTATTGCTACTGATATTTCACTTGAAGCAATGAATATGGCTCGTAATAATGCTGAGAAGCATCAATCACAAATACAAT
TTTTAACAGGGGATGCATTAAAGCCCTTAATTAATGAAGGTATCAAGTTGAACGGCTTGATATCTAATCCACCATATATA
GATGAAAAAGATATGGTTACGATGTCTCCAACGGTTACGAGATTCGAACCACATCAGGCATTGTTTGCAGATAACCATGG
ATATGCTATTTATGAATCAATCATTGAAGATTTACCTCACGTTATGGAAAAAGGCAGCCCAGTTGTTTTTGAAATTGGTT
ACAATCAAGGTGAGGCACTTAAATCAATAATTTTAAATAAATTTCCTGACAAAAAAATCGACATTATTAAAGATATAAAT
GGCCACGATCGAATCGTCTCATTTAAATGGTAA

Upstream 100 bases:

>100_bases
TTGAAAGAACTTAATAATGGTGAATTATAAAGAAAAGTTAGATGAAGCAATTCATTTAACACAACAAAAAGGGTTTGAAC
AAACACGAGCTGAATGGTTA

Downstream 100 bases:

>100_bases
TTAGAAGTTATGCCTTTGCTATGATTAGTTAAGTGCATAGCTTTTTGCTTTATATTATGATAAATAAGAAAGGCGTGATT
AAGTTGGATACTAAAATTTG

Product: HemK family modification methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MLDVFQWTRTDFVVHMHDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLHFLQQLE
DDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQIQFLTGDALKPLINEGIKLNGLISNPPYI
DEKDMVTMSPTVTRFEPHQALFADNHGYAIYESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDIN
GHDRIVSFKW

Sequences:

>Translated_250_residues
MLDVFQWTRTDFVVHMHDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLHFLQQLE
DDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQIQFLTGDALKPLINEGIKLNGLISNPPYI
DEKDMVTMSPTVTRFEPHQALFADNHGYAIYESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDIN
GHDRIVSFKW
>Mature_250_residues
MLDVFQWTRTDFVVHMHDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLHFLQQLE
DDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQIQFLTGDALKPLINEGIKLNGLISNPPYI
DEKDMVTMSPTVTRFEPHQALFADNHGYAIYESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDIN
GHDRIVSFKW

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=236, Percent_Identity=25.4237288135593, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1787463, Length=241, Percent_Identity=32.3651452282158, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI87082085, Length=210, Percent_Identity=26.1904761904762, Blast_Score=74, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24582226, Length=213, Percent_Identity=23.943661971831, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28342; Mature: 28342

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDVFQWTRTDFVVHMHDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSN
CCCCCEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCC
CLIPRPETEEVMLHFLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNM
EECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEECCCCCEEEEEECCHHHHHH
ARNNAEKHQSQIQFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQA
HHCCHHHHHHHHHHHCCHHHHHHHHCCEEEEEEECCCCCCCCCCEEEECCCEEEECCCCE
LFADNHGYAIYESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDIN
EEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHEECCC
GHDRIVSFKW
CCCCEEEECC
>Mature Secondary Structure
MLDVFQWTRTDFVVHMHDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSN
CCCCCEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCC
CLIPRPETEEVMLHFLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNM
EECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEECCCCCEEEEEECCHHHHHH
ARNNAEKHQSQIQFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQA
HHCCHHHHHHHHHHHCCHHHHHHHHCCEEEEEEECCCCCCCCCCEEEECCCEEEECCCCE
LFADNHGYAIYESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDIN
EEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHEECCC
GHDRIVSFKW
CCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]