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The map label for this gene is braB [H]

Identifier: 88194086

GI number: 88194086

Start: 297408

End: 298685

Strand: Reverse

Name: braB [H]

Synonym: SAOUHSC_00282

Alternate gene names: 88194086

Gene position: 298685-297408 (Counterclockwise)

Preceding gene: 88196673

Following gene: 88194085

Centisome position: 10.59

GC content: 35.92

Gene sequence:

>1278_bases
ATGTTATTTTCACTATTTTTTGGAGCCGGAAATTTAATATTCCCGCCCATGCTTGGCCATACAGCGGGTCAAAATATGTG
GATTGGTATGCTAGGCTTTGCCCTTACAGGCATATTACTCCCCTTTATTACTGTTATTGTTGTTGCATTTTATGATGAAG
GTGTTGAAAGTGTAGGCAATCGTATACATCCATGGTTCGGGTTTATTTTTGCTGTCGTGATTTACATGTCTATCGGAGCA
TTTTACGGTATTCCACGTGCTGCAAATGTCGCGTACGAAATTGGTACAAGACACATTTTACCTGTGCATAACCAATGGAC
TTTAATTATATTCGCAGCAATCTTTTTTGCCATCGTTTACTGGATTAGTTTAAATCCATCGAAAATCGTTGATAATTTAG
GTAAATTATTAACACCGTTATTACTATTAATGGTCGCTCTATTAAGTATTGCTGTCATTTTCAACCCTGAATCTGCACTA
AGTGCACCTAAGGATAAATATATAACACATCCTTTCATTTCAGGAAGTTTGGAAGGCTATTTTACAATGGATCTTGTTGC
TGCGTTAGCTTTTTCCGTAGTCATTGTCAATGGCTATAAGTTTAAAGGCCTCACAGATCGCATGAAAATTTTAAAATATG
TCTGCTTTTCAGGTCTTATTGCAGCCATATTACTTGGAATGATTTACTTTGCACTTGCATACGTTGGGGCATCAACAGCT
CCAGGAAACTTTAAAGATGGTACAGATATATTGACGTACAACTCATTACGATTATTTGGTTCGTTCGGTAACCTCGTATT
TGGAATGACGGTTATCCTTGCATGCCTAACAACATGTATAGGACTCGTCAATGCTTGCGCCACATTTACTAAGAAACACG
TACCTAAGTTTTCTTATAAAATATTCGCACTTATTTTCTCTATCATAGGGTTCTTATTTACAACACTTGGTTTAGAAATG
ATTTTAAAAATTGCTGTCCCATTATTGACTTTAATATATCCCGTGTCGATTGCACTTGTACTCATATCATTTGCTAACAT
GTTTAGCACATTCAGATTCAGTTGGGCCTATCGACTCGCAACTGTTATTACATTGATTATTTCAATTTTACAAATACTAA
ATAGTTTCAACTTATTACACGGTGTTATTTTGAAATCGTTTATGATGTTACCTTTAGCAGATATCGATTTAGCTTGGCTT
GTACCATTCATGCTCTTTGCTATTATCGGTTTCATAATCGATGTATTTATACGCCGTCCGAAACAAGCGACAACTTAA

Upstream 100 bases:

>100_bases
AGTAAGAACGTTTTTATTTTTCAGATTTTTTAAATTATTATGAATATCTAGTTTTAGGAAGGAAATTACATTGAAAAAAC
AAGTTATTATTTCGGGCCTC

Downstream 100 bases:

>100_bases
TAAATGCTCACTGCCTAGTAATGATTGACCCATCGTTACTAGGCTTTTTTATATGAAATTCATGACTTTTCAGTGATACC
GTCTGTCTATTGATTATTTT

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFALTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGA
FYGIPRAANVAYEIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSIAVIFNPESAL
SAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRMKILKYVCFSGLIAAILLGMIYFALAYVGASTA
PGNFKDGTDILTYNSLRLFGSFGNLVFGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEM
ILKIAVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGVILKSFMMLPLADIDLAWL
VPFMLFAIIGFIIDVFIRRPKQATT

Sequences:

>Translated_425_residues
MLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFALTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGA
FYGIPRAANVAYEIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSIAVIFNPESAL
SAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRMKILKYVCFSGLIAAILLGMIYFALAYVGASTA
PGNFKDGTDILTYNSLRLFGSFGNLVFGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEM
ILKIAVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGVILKSFMMLPLADIDLAWL
VPFMLFAIIGFIIDVFIRRPKQATT
>Mature_425_residues
MLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFALTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGA
FYGIPRAANVAYEIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSIAVIFNPESAL
SAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRMKILKYVCFSGLIAAILLGMIYFALAYVGASTA
PGNFKDGTDILTYNSLRLFGSFGNLVFGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEM
ILKIAVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGVILKSFMMLPLADIDLAWL
VPFMLFAIIGFIIDVFIRRPKQATT

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=428, Percent_Identity=34.8130841121495, Blast_Score=243, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46935; Mature: 46935

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFALTGILLPFITVIVVAFYDEGVESVGN
CEEEEEECCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RIHPWFGFIFAVVIYMSIGAFYGIPRAANVAYEIGTRHILPVHNQWTLIIFAAIFFAIVY
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHH
WISLNPSKIVDNLGKLLTPLLLLMVALLSIAVIFNPESALSAPKDKYITHPFISGSLEGY
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCH
FTMDLVAALAFSVVIVNGYKFKGLTDRMKILKYVCFSGLIAAILLGMIYFALAYVGASTA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PGNFKDGTDILTYNSLRLFGSFGNLVFGMTVILACLTTCIGLVNACATFTKKHVPKFSYK
CCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IFALIFSIIGFLFTTLGLEMILKIAVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVITLIISILQILNSFNLLHGVILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
KQATT
CCCCC
>Mature Secondary Structure
MLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFALTGILLPFITVIVVAFYDEGVESVGN
CEEEEEECCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RIHPWFGFIFAVVIYMSIGAFYGIPRAANVAYEIGTRHILPVHNQWTLIIFAAIFFAIVY
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHH
WISLNPSKIVDNLGKLLTPLLLLMVALLSIAVIFNPESALSAPKDKYITHPFISGSLEGY
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCH
FTMDLVAALAFSVVIVNGYKFKGLTDRMKILKYVCFSGLIAAILLGMIYFALAYVGASTA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PGNFKDGTDILTYNSLRLFGSFGNLVFGMTVILACLTTCIGLVNACATFTKKHVPKFSYK
CCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IFALIFSIIGFLFTTLGLEMILKIAVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVITLIISILQILNSFNLLHGVILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
KQATT
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]