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The map label for this gene is ywcJ [H]

Identifier: 88194085

GI number: 88194085

Start: 296347

End: 297171

Strand: Reverse

Name: ywcJ [H]

Synonym: SAOUHSC_00281

Alternate gene names: 88194085

Gene position: 297171-296347 (Counterclockwise)

Preceding gene: 88194086

Following gene: 88194062

Centisome position: 10.53

GC content: 33.33

Gene sequence:

>825_bases
GTGAAGGAGAAACATATTAAATGGGATAAGATTTTTTATGGGGACGACTGGGTAAATAACGTAGTCGAAACGATAAGAAC
AAAAGATATATTACAAAGCGTTTATTTAAAACGTTATTTATTACGCGCGATGATGGCAGGATTTATTATCGGGATTATTA
CGGTCTTCGTATTATCAGTTAAAGCAACACACGAACCAGATTTACCGCCAGGCATTGTGAATATGGCCAGTGCCATTACA
TTCAGCTTTGCGTTAGTACTCATTTTATTTACAAACTCCGAACTACTAACCAGTAACTTCATGTACTTTACTGTAGGCCT
GTATTATAAAGTAATTAAACCAACTAGAGTATTGAAAATATTTTTATTATGCTTTGCAGGAAATATTTTAGGTGCTGCTA
TTTTATTTAGTTTCATGCGTTTTTCAAATGTAATGACGCCAGATATGTTAAATCAGTTATCAGCAGTTATAGAGCATAAA
ACGTTGTCTACTGGTTTTGTAAGTATATTAATGAAAGCGATCTTCGCCAATTTCTTTATCAATATTTCATTAGTTATTGC
CATGCAAATTGACGATGTACTCGCAAAAATGTTTGTCATGATGTTCGGTGTTACCATATTCGCCTTTATGGGTTATGAAC
ACGTCGTTTACAACAGTTGCTTATTTATGGGTGGTTTAATTTATCAAGTTGATACATTGCACTTCATACCAGCTATTTCG
AATATCGCTGCGGCTTTTATCGGTAACTATATTGGAGGCGGACTCATTATAGGTTTATTCTATGCTTACTTGAATGATCA
TCATCAATTTTATAAAAATAATTAA

Upstream 100 bases:

>100_bases
TCAAAAATATTAGTTTTTAAAATTAATTTTGTGAAATTATTCACAAATAAGGCAATTCATAGTATACTCTGATTAACAAA
TGAAAAGTGAGGATGTCATT

Downstream 100 bases:

>100_bases
CTATATTTGAACAATGCGTTAGTCGATGCCTTTAATATTTAGAAGTGCATTGTCTAACGTATTTTTTTAAAGTTCGTCAA
TGATTTTTATCCGATGCTAT

Product: hypothetical protein

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAMMAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAIT
FSFALVLILFTNSELLTSNFMYFTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYNSCLFMGGLIYQVDTLHFIPAIS
NIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN

Sequences:

>Translated_274_residues
MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAMMAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAIT
FSFALVLILFTNSELLTSNFMYFTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYNSCLFMGGLIYQVDTLHFIPAIS
NIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN
>Mature_274_residues
MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAMMAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAIT
FSFALVLILFTNSELLTSNFMYFTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYNSCLFMGGLIYQVDTLHFIPAIS
NIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN

Specific function: Involved In The Bidirectional Transport Of Formate. [C]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=268, Percent_Identity=27.9850746268657, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 31019; Mature: 31019

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2163394; 7934828; 9384377 [H]