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The map label for this gene is pbpG [H]

Identifier: 87201341

GI number: 87201341

Start: 3548577

End: 3550715

Strand: Direct

Name: pbpG [H]

Synonym: Saro_3329

Alternate gene names: 87201341

Gene position: 3548577-3550715 (Clockwise)

Preceding gene: 87201340

Following gene: 87201342

Centisome position: 99.63

GC content: 67.32

Gene sequence:

>2139_bases
ATGGCCAGACGGGACATTCCCCGCCGCCCCGAAGGCGGCAGGTCCGCTCCGCAACCCTCCGGTCCCTCGGCGCTTCGCCG
CTGGGTGGGAAGGCTGTTTGCCTGGGGCATCGGATTCGCGCTGCTTGGCGCCATAGCGCTGATAGTGGCCGTCGCGGTCA
CCGCGCGCTCGCTGCCCGGCTACCAGGAGCTGAAGTCCAGCCAGACCGGGCAGATGGTGGTCGTGCGGGCCGCCGACGGT
TCCGAAATCGTGACGCTCGGCCCCAGCTACGGCAAGTGGGTTCCCTATGAGCGCATCCCCGAGGTGATGAAGGACGCCAT
GACCTCGGTCGAGGACAGGCGCTTCCGCAGCCACTGGGGCGTGGACCCGATCGGGATCGTGCGATCGCTGATGGTGCGCG
TGGAGAGCGGCTACTGGAAGCAGGGCGGCTCGACCATCACACAGCAGCTTGCCCGCAACATCTTCCTCAACAATACCAAG
ACCTTCGGCCGCAAGGCGCGGGAATGGGTGCTGGCGCTGGCGCTGGAGCAGAAGTTCTCGAAGGACCAGATCCTCGAGCT
GTACCTCAACAAGGTCTACTTCGGCGGCGGCGCCTATGGCATCGATTCGGCCGCACGCAAGTTCTTCGGCCATTCGGGCG
AGGAGATCACCCTGCCCGAGGCCGCGATCATCGCCGGCCTGGTCAAGGCACCGTCCCGCTATTCGCCCACGGCCGATGCC
GATGCCGCCGTCGGTCGCGCCGGCGTGGTGCTGCAGACGATGCAGGACAACGGCGTGCTGACCGCGCAGGAAGCCGCCGC
CGTCGACCTCAGGAAGGTCAAGATGGTGGAGGACCGCGCCAGCCAGAACTCGGCGCGCTACTTCACCGACTGGGCGTTGC
CGCAACTCGACCTGATCCTGCCCGAGAACGACCAGCCGATCGAGGTTTGGACCACGCTCGATCCGGTGATGCAGCGCGCC
GCGACGAGCGCCATTGCCGGCTTCGCGCCGAAGGGCGCGCAAGGCGCGCTTGTCTCGGTGGACCGCGACGGCGCCGTCCT
CGCGATGGTCGGCGGGACCGACTACGTCAGCTCGAACTACAACCGCGCGGTAAACGCCGTCCGCCAGCCGGGTTCGGCGT
GGAAGCTGTTCGTCTACCTCGCCGCGCTCGAGGCCGGATATACCCCCGACGACCGCGTGGTCGACGAGCCGGTGACGATC
GACGGCTGGACGCCGCGCAACGACGGCGCGCGCTATGCTGGCGAGATCGACCTGCGCACCGCATTTGCCTATTCCAAGAA
CACCGTTGCAGCACAACTCGGCAACGAGGTGGGCTTCGGCGCGGTGGCCAACATGGCACGGCGCTTCGGCATCACCACGC
CGATCAACACCTTGCCCTCGATGGTCCTTGGATCGTCGGAAGTTCGGGTGATCGACATGGTCCGCGCCTTCGCCTCGGTC
CAGGCCAAGGGCAATTCGGTGACCCCCTATGGCATCCGCAAGGTGACGACGACCGATGGCGAAGTGCTCTACAAGCACCG
CGCCGATGCCTCGCAGCAGCTCGTCCCGCCCTATGTCGCGGCGGGCATGACCGATCTCATGCAGACCGCGGTGAATATCG
GCACTGGCCGCGCCGCGCAGATCGGACGACCCGTCGCAGGCAAGACCGGCACCACCTCTTCCAACAAGGACGGCTGGTTC
CTCGGTTTCTCCAGTGGCATCACCACGGGCGTATGGATGGGCCGTGACGATGCCAAGCCGGTGCCCGGCCTCCAGGGCGG
CGCCGCGCCCGCGCGCGCATTCGCTGCCTACATGAAGGTCGCCACGGCCAAGCGCCCGTCCGAAAAGTTCGATACCGAAC
TCAAGCTGCCGCAATGGCAGCTCGAACCCGACGACGAGGCGATGCAGGGCAATCCCGACGATTACTTCTATGTCGACGAA
CAGGGCAACCTTGTGGACCCGTCACGTCGCCCATCGCCCGGCGCCAATCCCGACGCCGTCGATCCGGATGCGCTGCCCGA
AGGCGTGCCCCCGGATGCGCCCCAGGCGGCGACCGACGATTTCCTCAACCGCGCCACGGGGCGGCAGGGAGACACGCCAC
CCGCGACAGGACCGCGCACCCGTCCGACGAGCAGCGCCCGGACGGTGACCGTTCCCTGA

Upstream 100 bases:

>100_bases
CTTCGGCGATTTGCGGTTAACCCTGCGTTCCGATTGCCCTATGCATTGGGCCGTGACGATGCGCCTGCCTGCGCCGGATT
GAGGAAGCGACAAGAGACTG

Downstream 100 bases:

>100_bases
ACTTTTTGCCCGATTGATCGTTGCGAGTCCGATGGAAGGCGTCTGCCTTCCGACAGAGGAGACTTGCAATGAGCCTGCTG
CAATGGGCCGTCGTCGGCGC

Product: penicillin-binding protein 1A

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 712; Mature: 711

Protein sequence:

>712_residues
MARRDIPRRPEGGRSAPQPSGPSALRRWVGRLFAWGIGFALLGAIALIVAVAVTARSLPGYQELKSSQTGQMVVVRAADG
SEIVTLGPSYGKWVPYERIPEVMKDAMTSVEDRRFRSHWGVDPIGIVRSLMVRVESGYWKQGGSTITQQLARNIFLNNTK
TFGRKAREWVLALALEQKFSKDQILELYLNKVYFGGGAYGIDSAARKFFGHSGEEITLPEAAIIAGLVKAPSRYSPTADA
DAAVGRAGVVLQTMQDNGVLTAQEAAAVDLRKVKMVEDRASQNSARYFTDWALPQLDLILPENDQPIEVWTTLDPVMQRA
ATSAIAGFAPKGAQGALVSVDRDGAVLAMVGGTDYVSSNYNRAVNAVRQPGSAWKLFVYLAALEAGYTPDDRVVDEPVTI
DGWTPRNDGARYAGEIDLRTAFAYSKNTVAAQLGNEVGFGAVANMARRFGITTPINTLPSMVLGSSEVRVIDMVRAFASV
QAKGNSVTPYGIRKVTTTDGEVLYKHRADASQQLVPPYVAAGMTDLMQTAVNIGTGRAAQIGRPVAGKTGTTSSNKDGWF
LGFSSGITTGVWMGRDDAKPVPGLQGGAAPARAFAAYMKVATAKRPSEKFDTELKLPQWQLEPDDEAMQGNPDDYFYVDE
QGNLVDPSRRPSPGANPDAVDPDALPEGVPPDAPQAATDDFLNRATGRQGDTPPATGPRTRPTSSARTVTVP

Sequences:

>Translated_712_residues
MARRDIPRRPEGGRSAPQPSGPSALRRWVGRLFAWGIGFALLGAIALIVAVAVTARSLPGYQELKSSQTGQMVVVRAADG
SEIVTLGPSYGKWVPYERIPEVMKDAMTSVEDRRFRSHWGVDPIGIVRSLMVRVESGYWKQGGSTITQQLARNIFLNNTK
TFGRKAREWVLALALEQKFSKDQILELYLNKVYFGGGAYGIDSAARKFFGHSGEEITLPEAAIIAGLVKAPSRYSPTADA
DAAVGRAGVVLQTMQDNGVLTAQEAAAVDLRKVKMVEDRASQNSARYFTDWALPQLDLILPENDQPIEVWTTLDPVMQRA
ATSAIAGFAPKGAQGALVSVDRDGAVLAMVGGTDYVSSNYNRAVNAVRQPGSAWKLFVYLAALEAGYTPDDRVVDEPVTI
DGWTPRNDGARYAGEIDLRTAFAYSKNTVAAQLGNEVGFGAVANMARRFGITTPINTLPSMVLGSSEVRVIDMVRAFASV
QAKGNSVTPYGIRKVTTTDGEVLYKHRADASQQLVPPYVAAGMTDLMQTAVNIGTGRAAQIGRPVAGKTGTTSSNKDGWF
LGFSSGITTGVWMGRDDAKPVPGLQGGAAPARAFAAYMKVATAKRPSEKFDTELKLPQWQLEPDDEAMQGNPDDYFYVDE
QGNLVDPSRRPSPGANPDAVDPDALPEGVPPDAPQAATDDFLNRATGRQGDTPPATGPRTRPTSSARTVTVP
>Mature_711_residues
ARRDIPRRPEGGRSAPQPSGPSALRRWVGRLFAWGIGFALLGAIALIVAVAVTARSLPGYQELKSSQTGQMVVVRAADGS
EIVTLGPSYGKWVPYERIPEVMKDAMTSVEDRRFRSHWGVDPIGIVRSLMVRVESGYWKQGGSTITQQLARNIFLNNTKT
FGRKAREWVLALALEQKFSKDQILELYLNKVYFGGGAYGIDSAARKFFGHSGEEITLPEAAIIAGLVKAPSRYSPTADAD
AAVGRAGVVLQTMQDNGVLTAQEAAAVDLRKVKMVEDRASQNSARYFTDWALPQLDLILPENDQPIEVWTTLDPVMQRAA
TSAIAGFAPKGAQGALVSVDRDGAVLAMVGGTDYVSSNYNRAVNAVRQPGSAWKLFVYLAALEAGYTPDDRVVDEPVTID
GWTPRNDGARYAGEIDLRTAFAYSKNTVAAQLGNEVGFGAVANMARRFGITTPINTLPSMVLGSSEVRVIDMVRAFASVQ
AKGNSVTPYGIRKVTTTDGEVLYKHRADASQQLVPPYVAAGMTDLMQTAVNIGTGRAAQIGRPVAGKTGTTSSNKDGWFL
GFSSGITTGVWMGRDDAKPVPGLQGGAAPARAFAAYMKVATAKRPSEKFDTELKLPQWQLEPDDEAMQGNPDDYFYVDEQ
GNLVDPSRRPSPGANPDAVDPDALPEGVPPDAPQAATDDFLNRATGRQGDTPPATGPRTRPTSSARTVTVP

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=536, Percent_Identity=31.9029850746269, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI87082258, Length=238, Percent_Identity=42.0168067226891, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1788867, Length=531, Percent_Identity=29.0018832391714, Blast_Score=160, Evalue=3e-40,
Organism=Escherichia coli, GI1789601, Length=170, Percent_Identity=32.3529411764706, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 76442; Mature: 76311

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRDIPRRPEGGRSAPQPSGPSALRRWVGRLFAWGIGFALLGAIALIVAVAVTARSLPG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YQELKSSQTGQMVVVRAADGSEIVTLGPSYGKWVPYERIPEVMKDAMTSVEDRRFRSHWG
HHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
VDPIGIVRSLMVRVESGYWKQGGSTITQQLARNIFLNNTKTFGRKAREWVLALALEQKFS
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
KDQILELYLNKVYFGGGAYGIDSAARKFFGHSGEEITLPEAAIIAGLVKAPSRYSPTADA
HHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCCCCCCCCC
DAAVGRAGVVLQTMQDNGVLTAQEAAAVDLRKVKMVEDRASQNSARYFTDWALPQLDLIL
HHHCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEE
PENDQPIEVWTTLDPVMQRAATSAIAGFAPKGAQGALVSVDRDGAVLAMVGGTDYVSSNY
CCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEEECCCHHHHCCH
NRAVNAVRQPGSAWKLFVYLAALEAGYTPDDRVVDEPVTIDGWTPRNDGARYAGEIDLRT
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCEECEEEEEE
AFAYSKNTVAAQLGNEVGFGAVANMARRFGITTPINTLPSMVLGSSEVRVIDMVRAFASV
EEHHCCCHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
QAKGNSVTPYGIRKVTTTDGEVLYKHRADASQQLVPPYVAAGMTDLMQTAVNIGTGRAAQ
HCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
IGRPVAGKTGTTSSNKDGWFLGFSSGITTGVWMGRDDAKPVPGLQGGAAPARAFAAYMKV
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
ATAKRPSEKFDTELKLPQWQLEPDDEAMQGNPDDYFYVDEQGNLVDPSRRPSPGANPDAV
HHCCCCCHHCCCCCCCCCCCCCCCCHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
DPDALPEGVPPDAPQAATDDFLNRATGRQGDTPPATGPRTRPTSSARTVTVP
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECC
>Mature Secondary Structure 
ARRDIPRRPEGGRSAPQPSGPSALRRWVGRLFAWGIGFALLGAIALIVAVAVTARSLPG
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YQELKSSQTGQMVVVRAADGSEIVTLGPSYGKWVPYERIPEVMKDAMTSVEDRRFRSHWG
HHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
VDPIGIVRSLMVRVESGYWKQGGSTITQQLARNIFLNNTKTFGRKAREWVLALALEQKFS
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
KDQILELYLNKVYFGGGAYGIDSAARKFFGHSGEEITLPEAAIIAGLVKAPSRYSPTADA
HHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCCCCCCCCC
DAAVGRAGVVLQTMQDNGVLTAQEAAAVDLRKVKMVEDRASQNSARYFTDWALPQLDLIL
HHHCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEE
PENDQPIEVWTTLDPVMQRAATSAIAGFAPKGAQGALVSVDRDGAVLAMVGGTDYVSSNY
CCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEEECCCHHHHCCH
NRAVNAVRQPGSAWKLFVYLAALEAGYTPDDRVVDEPVTIDGWTPRNDGARYAGEIDLRT
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCEECEEEEEE
AFAYSKNTVAAQLGNEVGFGAVANMARRFGITTPINTLPSMVLGSSEVRVIDMVRAFASV
EEHHCCCHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
QAKGNSVTPYGIRKVTTTDGEVLYKHRADASQQLVPPYVAAGMTDLMQTAVNIGTGRAAQ
HCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
IGRPVAGKTGTTSSNKDGWFLGFSSGITTGVWMGRDDAKPVPGLQGGAAPARAFAAYMKV
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
ATAKRPSEKFDTELKLPQWQLEPDDEAMQGNPDDYFYVDEQGNLVDPSRRPSPGANPDAV
HHCCCCCHHCCCCCCCCCCCCCCCCHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
DPDALPEGVPPDAPQAATDDFLNRATGRQGDTPPATGPRTRPTSSARTVTVP
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]