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The map label for this gene is rffG [H]

Identifier: 87201247

GI number: 87201247

Start: 3456553

End: 3457611

Strand: Reverse

Name: rffG [H]

Synonym: Saro_3235

Alternate gene names: 87201247

Gene position: 3457611-3456553 (Counterclockwise)

Preceding gene: 87201248

Following gene: 87201245

Centisome position: 97.08

GC content: 63.08

Gene sequence:

>1059_bases
TTGTCTAACCTGCTTGTCACCGGCGGCGCCGGCTTCATCGGCGGCAATTTCGTCCACTATTGGGCGCAGCAGCACCCTGA
CGACACGATCGTCGTGCTCGACTGCCTGACTTATGCTGGCAACCGGTCGACCATTGCGGGGGTGGAACAGGCCGAACTCG
TGGTGGGCGACATTCGCGACACCGACCTCGTCGAGAAGCTGCTGCGCGAGCGGGACATCGCGACGCTCGTCCACTTCGCC
GCAGAAAGCCATGTCGACCGTTCGATTACCGGACCGGACGCCTTCATCGAAACCAATATCCTTGGGACCAACAGCCTGCT
CAAGGCCGCGCGCAAGGTCTGGCTGGACGAAGGTTCGGGCCGCGCCCACCGCTTCCACCACATCTCGACCGACGAAGTAT
ACGGGTCGCTCGGTCCCAGCGATCCGGCCTTCTCGGAAACCACGCAGTACCAGCCGAACTCGCCCTATTCGGCGTCGAAG
GCCGCATCGGACCACCTCGTGCGCGCCTATCACCATACCTATGGTCTGGATGTGACGACGACGAACTGTTCGAACAATTA
TGGGCCGTACCATTACCCGGAAAAGCTGATCCCGCTGTTCATCCTCAACGCGCTGTCGGGCAAGCCGCTGCCGATCTACG
GCGACGGCATGAACGTGCGCGACTGGCTTTACGTCGAGGACCACTGCCGGGGAATCGAGGCGGCGCTGAAGAACGGCAAG
GCCGGCGAGACCTACAACATCGGTGGCGGCGAGGAACTGCCCAACATGGCGGTTATCGACCGTATCTGCGCGGAAGTGGA
TCGGGCATTCGTCGAAGTCGAGGGGCTTGCGGAGCGTTATCCGGATGCGCCCGCCGCCAAGGGCCGGGCGACCAGCGAAC
TCAAGACCTTCGTCGAGGACCGCAAGGGGCACGATCGCCGATATGCAATCGACGAGACCAAGGCGCGTGCGGAGCTGGGC
TATGTGCCGCAGCACGACTTCGAGACAGGCCTTCGCGGCACCCTGCGCTGGTACTTCGACAACGAAGCGTGGTGGCGGCC
GCTCAAGGATCGCGGCTGA

Upstream 100 bases:

>100_bases
TTCTCGGTGCTCGACGTCAGCGGTACGCGAAGCCTGCTGGGCGACGCGCACGTTCATTGGCGCACCAATCTCAAGACAAT
GCTGAAAGAGGAAAAGGCTC

Downstream 100 bases:

>100_bases
TCTGCCCGGCTGGACCGGTTCCCGATCGGGCCGGTCTAGCCATTCGCCGCGTCGCGGTTGCCTTCGTCGAGTGCGCGGGC
GGTCGCCTCTAGATTGGCGT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSNLLVTGGAGFIGGNFVHYWAQQHPDDTIVVLDCLTYAGNRSTIAGVEQAELVVGDIRDTDLVEKLLRERDIATLVHFA
AESHVDRSITGPDAFIETNILGTNSLLKAARKVWLDEGSGRAHRFHHISTDEVYGSLGPSDPAFSETTQYQPNSPYSASK
AASDHLVRAYHHTYGLDVTTTNCSNNYGPYHYPEKLIPLFILNALSGKPLPIYGDGMNVRDWLYVEDHCRGIEAALKNGK
AGETYNIGGGEELPNMAVIDRICAEVDRAFVEVEGLAERYPDAPAAKGRATSELKTFVEDRKGHDRRYAIDETKARAELG
YVPQHDFETGLRGTLRWYFDNEAWWRPLKDRG

Sequences:

>Translated_352_residues
MSNLLVTGGAGFIGGNFVHYWAQQHPDDTIVVLDCLTYAGNRSTIAGVEQAELVVGDIRDTDLVEKLLRERDIATLVHFA
AESHVDRSITGPDAFIETNILGTNSLLKAARKVWLDEGSGRAHRFHHISTDEVYGSLGPSDPAFSETTQYQPNSPYSASK
AASDHLVRAYHHTYGLDVTTTNCSNNYGPYHYPEKLIPLFILNALSGKPLPIYGDGMNVRDWLYVEDHCRGIEAALKNGK
AGETYNIGGGEELPNMAVIDRICAEVDRAFVEVEGLAERYPDAPAAKGRATSELKTFVEDRKGHDRRYAIDETKARAELG
YVPQHDFETGLRGTLRWYFDNEAWWRPLKDRG
>Mature_351_residues
SNLLVTGGAGFIGGNFVHYWAQQHPDDTIVVLDCLTYAGNRSTIAGVEQAELVVGDIRDTDLVEKLLRERDIATLVHFAA
ESHVDRSITGPDAFIETNILGTNSLLKAARKVWLDEGSGRAHRFHHISTDEVYGSLGPSDPAFSETTQYQPNSPYSASKA
ASDHLVRAYHHTYGLDVTTTNCSNNYGPYHYPEKLIPLFILNALSGKPLPIYGDGMNVRDWLYVEDHCRGIEAALKNGKA
GETYNIGGGEELPNMAVIDRICAEVDRAFVEVEGLAERYPDAPAAKGRATSELKTFVEDRKGHDRRYAIDETKARAELGY
VPQHDFETGLRGTLRWYFDNEAWWRPLKDRG

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=346, Percent_Identity=33.8150289017341, Blast_Score=194, Evalue=7e-50,
Organism=Homo sapiens, GI42516563, Length=336, Percent_Identity=27.0833333333333, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI48994969, Length=355, Percent_Identity=54.0845070422535, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1788353, Length=354, Percent_Identity=52.5423728813559, Blast_Score=346, Evalue=2e-96,
Organism=Escherichia coli, GI1786974, Length=343, Percent_Identity=26.2390670553936, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788366, Length=215, Percent_Identity=25.1162790697674, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17568069, Length=342, Percent_Identity=33.6257309941521, Blast_Score=183, Evalue=9e-47,
Organism=Caenorhabditis elegans, GI115532424, Length=339, Percent_Identity=31.5634218289086, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17539532, Length=257, Percent_Identity=26.4591439688716, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21356223, Length=348, Percent_Identity=25.8620689655172, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19923002, Length=373, Percent_Identity=24.9329758713137, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39143; Mature: 39012

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLLVTGGAGFIGGNFVHYWAQQHPDDTIVVLDCLTYAGNRSTIAGVEQAELVVGDIRD
CCCEEEECCCCCCCCCHHHHHHHCCCCCEEEEEEEHHHCCCCCEECCCCHHHEEEECCCC
TDLVEKLLRERDIATLVHFAAESHVDRSITGPDAFIETNILGTNSLLKAARKVWLDEGSG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCC
RAHRFHHISTDEVYGSLGPSDPAFSETTQYQPNSPYSASKAASDHLVRAYHHTYGLDVTT
CCEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEE
TNCSNNYGPYHYPEKLIPLFILNALSGKPLPIYGDGMNVRDWLYVEDHCRGIEAALKNGK
ECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEHHHCCHHHHHHCCCC
AGETYNIGGGEELPNMAVIDRICAEVDRAFVEVEGLAERYPDAPAAKGRATSELKTFVED
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
RKGHDRRYAIDETKARAELGYVPQHDFETGLRGTLRWYFDNEAWWRPLKDRG
HCCCCCEEECHHHHHHHHCCCCCCCCHHCCCCEEEEEEECCHHHCCCCCCCC
>Mature Secondary Structure 
SNLLVTGGAGFIGGNFVHYWAQQHPDDTIVVLDCLTYAGNRSTIAGVEQAELVVGDIRD
CCEEEECCCCCCCCCHHHHHHHCCCCCEEEEEEEHHHCCCCCEECCCCHHHEEEECCCC
TDLVEKLLRERDIATLVHFAAESHVDRSITGPDAFIETNILGTNSLLKAARKVWLDEGSG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCC
RAHRFHHISTDEVYGSLGPSDPAFSETTQYQPNSPYSASKAASDHLVRAYHHTYGLDVTT
CCEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEE
TNCSNNYGPYHYPEKLIPLFILNALSGKPLPIYGDGMNVRDWLYVEDHCRGIEAALKNGK
ECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEHHHCCHHHHHHCCCC
AGETYNIGGGEELPNMAVIDRICAEVDRAFVEVEGLAERYPDAPAAKGRATSELKTFVED
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
RKGHDRRYAIDETKARAELGYVPQHDFETGLRGTLRWYFDNEAWWRPLKDRG
HCCCCCEEECHHHHHHHHCCCCCCCCHHCCCCEEEEEEECCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]