Click here to switch to the map view.

The map label for this gene is 87201194

Identifier: 87201194

GI number: 87201194

Start: 3402497

End: 3403762

Strand: Reverse

Name: 87201194

Synonym: Saro_3182

Alternate gene names: NA

Gene position: 3403762-3402497 (Counterclockwise)

Preceding gene: 87201199

Following gene: 87201190

Centisome position: 95.57

GC content: 65.01

Gene sequence:

>1266_bases
GTGTTCGCAGTGCTCTACAAGGCTTACGAGATTCAGCGTTCCTTGATGAACGCGGGCAGCGCCTGGGCTTCCATGGCCGC
CGAGATTATCAACGACGCGCGCAATCCCTGGTCCGGCTTCGGCGGCAACCAGATGCTCGCTTCCGCGCTCGACGTCTTCG
CCCACGCCGCCGCGCCGCGCGGCAAGCCCGCTTTCGGGATCAAGGTCGTCCACGTCGATGGCGCGGCCCATGCGGTCAAG
GAAACGACCGTCATCACCCGCCCGTTCGGCGATCTCAAGATGTTCACGCACGATGGCCTGCCCAAGGACGCGCCGCGCCT
GCTGATCGTCGCGCCGATGAGCGGCCACTATGCCACGTTGCTGCGCGGCACGGTCGAACGCATGCTCGAACGCTGCGTGG
TCTACATCACCGACTGGGCCGATGCGAAGTACGTGCCGCTCGCCGAAGGCATCTTCGACCTCGACGACTACATCGACTAC
CTGACCGGCTTCCTCGAGCATATCGGCGAAGGGGCGCACATGATGGCGGTGTGCCAGCCCTCGGTCCCGGCCTTCGCCGC
CACTGCCATCATGGCCGCGAAGAAGCACCCCTGCCGTCCGGTCACGCTGACCATGATGGGCGGCCCGATCGATACGCGCG
AAAGCCCGACGGCGGTCAACGACCATGCCATCACCAAGCCGTATGTCTGGTTCAAGCACAACGTGATCACCACCGTGCCG
ACCAACTATCCGGGCGAGGGGCGGCGGGTCTATCCGGGCTTCGTCCAGCTTGCCAGCTTCATGTCGATGAACCTCGGCAG
CCACATGATGAGCCATTACAAGCTGTTCCAGCACATGATGGCCGGCGCCGACGAAAGCGCCGAGGCGACCAAGGCGTTCT
ACGACGAATACCGCGCGGTCTGCGATCTCCCGGCGGAGTTCTACCTGCAGACCGTGGACGTGGTCTTCCAGCGCCACGCC
CTGCCCAAGGGCGAGTTCGTCCATCGCGGTCAGGCGGTCGATCTCGGCGCGATCGCCGACACCGCGATCCTGTGCATCGA
GGGCGAGCGGGACGACATTTCGGGCATCGGCCAGACGAGGGCCGCGCTCAACGTCACGCCGAACCTCCCGGACGACATGA
AGCAATACCTGCTCGCGCCGGAAGTCGGCCACTACGGCATCTTCAACGGTTCGCGCTGGCGCGGCTCCATCGCCCCGGTG
GTGGAACAGTGGATCGCGAAGTTCGAGAAGAAGGCAAGGGGCCGCAAGCTGAGCGTGGTGGCCTGA

Upstream 100 bases:

>100_bases
GCGAGGGGCAGGGCCGATTTGTTGCGTTGCACAAGTGCTCGAATCCCGGCAATCTGGGCTCAACGACTCTCCGTCAGGTG
CGGGGCAACAAATCGAGAAG

Downstream 100 bases:

>100_bases
GGCGCTTGACCGGCGAATGCGCATCGTGCGTCTTCGCCGGTCCGATACCCGAACCCTACCTGAAAGACCTCTAACGCACC
CTGTAGAAGCGTATTCGCAC

Product: polyhydroxyalkanoate depolymerase, intracellular

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; PHB De-Polymerase Domain-Containing Protein; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Intracellular Polyhydroxyalkanoate Depolymerase; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Poly(3-Hydroxyalkanoate) Depolymerase; Poly(3-Hydroxybutyrate) Depolymerase; Poly(3-Hydroxyalkanoate)Synthetase; Alpha/Beta Hydrolase Fold; Alpha/Beta Hydrolase Fold Protein; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; PHB De-Polymerase Domain Protein; PHB De-Polymerase; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Polyhydroxybutanoate Depolymerase; Poly-Beta-Hydroxybutyrate Depolymerase

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MFAVLYKAYEIQRSLMNAGSAWASMAAEIINDARNPWSGFGGNQMLASALDVFAHAAAPRGKPAFGIKVVHVDGAAHAVK
ETTVITRPFGDLKMFTHDGLPKDAPRLLIVAPMSGHYATLLRGTVERMLERCVVYITDWADAKYVPLAEGIFDLDDYIDY
LTGFLEHIGEGAHMMAVCQPSVPAFAATAIMAAKKHPCRPVTLTMMGGPIDTRESPTAVNDHAITKPYVWFKHNVITTVP
TNYPGEGRRVYPGFVQLASFMSMNLGSHMMSHYKLFQHMMAGADESAEATKAFYDEYRAVCDLPAEFYLQTVDVVFQRHA
LPKGEFVHRGQAVDLGAIADTAILCIEGERDDISGIGQTRAALNVTPNLPDDMKQYLLAPEVGHYGIFNGSRWRGSIAPV
VEQWIAKFEKKARGRKLSVVA

Sequences:

>Translated_421_residues
MFAVLYKAYEIQRSLMNAGSAWASMAAEIINDARNPWSGFGGNQMLASALDVFAHAAAPRGKPAFGIKVVHVDGAAHAVK
ETTVITRPFGDLKMFTHDGLPKDAPRLLIVAPMSGHYATLLRGTVERMLERCVVYITDWADAKYVPLAEGIFDLDDYIDY
LTGFLEHIGEGAHMMAVCQPSVPAFAATAIMAAKKHPCRPVTLTMMGGPIDTRESPTAVNDHAITKPYVWFKHNVITTVP
TNYPGEGRRVYPGFVQLASFMSMNLGSHMMSHYKLFQHMMAGADESAEATKAFYDEYRAVCDLPAEFYLQTVDVVFQRHA
LPKGEFVHRGQAVDLGAIADTAILCIEGERDDISGIGQTRAALNVTPNLPDDMKQYLLAPEVGHYGIFNGSRWRGSIAPV
VEQWIAKFEKKARGRKLSVVA
>Mature_421_residues
MFAVLYKAYEIQRSLMNAGSAWASMAAEIINDARNPWSGFGGNQMLASALDVFAHAAAPRGKPAFGIKVVHVDGAAHAVK
ETTVITRPFGDLKMFTHDGLPKDAPRLLIVAPMSGHYATLLRGTVERMLERCVVYITDWADAKYVPLAEGIFDLDDYIDY
LTGFLEHIGEGAHMMAVCQPSVPAFAATAIMAAKKHPCRPVTLTMMGGPIDTRESPTAVNDHAITKPYVWFKHNVITTVP
TNYPGEGRRVYPGFVQLASFMSMNLGSHMMSHYKLFQHMMAGADESAEATKAFYDEYRAVCDLPAEFYLQTVDVVFQRHA
LPKGEFVHRGQAVDLGAIADTAILCIEGERDDISGIGQTRAALNVTPNLPDDMKQYLLAPEVGHYGIFNGSRWRGSIAPV
VEQWIAKFEKKARGRKLSVVA

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 46269; Mature: 46269

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAVLYKAYEIQRSLMNAGSAWASMAAEIINDARNPWSGFGGNQMLASALDVFAHAAAPR
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCC
GKPAFGIKVVHVDGAAHAVKETTVITRPFGDLKMFTHDGLPKDAPRLLIVAPMSGHYATL
CCCCCEEEEEEECCCHHHHHHCEEEECCCCCCEEEECCCCCCCCCCEEEEECCCCCHHHH
LRGTVERMLERCVVYITDWADAKYVPLAEGIFDLDDYIDYLTGFLEHIGEGAHMMAVCQP
HHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
SVPAFAATAIMAAKKHPCRPVTLTMMGGPIDTRESPTAVNDHAITKPYVWFKHNVITTVP
CCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCEEEECC
TNYPGEGRRVYPGFVQLASFMSMNLGSHMMSHYKLFQHMMAGADESAEATKAFYDEYRAV
CCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
CDLPAEFYLQTVDVVFQRHALPKGEFVHRGQAVDLGAIADTAILCIEGERDDISGIGQTR
HCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCEEECHHCCEEEEEEECCCCCCCCCCCCC
AALNVTPNLPDDMKQYLLAPEVGHYGIFNGSRWRGSIAPVVEQWIAKFEKKARGRKLSVV
EEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEE
A
C
>Mature Secondary Structure
MFAVLYKAYEIQRSLMNAGSAWASMAAEIINDARNPWSGFGGNQMLASALDVFAHAAAPR
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCC
GKPAFGIKVVHVDGAAHAVKETTVITRPFGDLKMFTHDGLPKDAPRLLIVAPMSGHYATL
CCCCCEEEEEEECCCHHHHHHCEEEECCCCCCEEEECCCCCCCCCCEEEEECCCCCHHHH
LRGTVERMLERCVVYITDWADAKYVPLAEGIFDLDDYIDYLTGFLEHIGEGAHMMAVCQP
HHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
SVPAFAATAIMAAKKHPCRPVTLTMMGGPIDTRESPTAVNDHAITKPYVWFKHNVITTVP
CCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCEEEECC
TNYPGEGRRVYPGFVQLASFMSMNLGSHMMSHYKLFQHMMAGADESAEATKAFYDEYRAV
CCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
CDLPAEFYLQTVDVVFQRHALPKGEFVHRGQAVDLGAIADTAILCIEGERDDISGIGQTR
HCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCEEECHHCCEEEEEEECCCCCCCCCCCCC
AALNVTPNLPDDMKQYLLAPEVGHYGIFNGSRWRGSIAPVVEQWIAKFEKKARGRKLSVV
EEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEE
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA