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The map label for this gene is pimA [H]

Identifier: 87201190

GI number: 87201190

Start: 3398543

End: 3399751

Strand: Reverse

Name: pimA [H]

Synonym: Saro_3178

Alternate gene names: 87201190

Gene position: 3399751-3398543 (Counterclockwise)

Preceding gene: 87201194

Following gene: 87201189

Centisome position: 95.46

GC content: 68.73

Gene sequence:

>1209_bases
ATGACCCGCGTGCTTCATGTTCTCGACCATTCGCTGCCGCTCCACAGCGGCTATACCTTCCGCACCCGCGCCATTCTGAA
GGCGCAGGAGGCGATGGGCATCGAGGTGCGTGGCGTCACCGGCCGGCGCCATGTCGCCCCTCCCGCCGCGCAGGAGCCGG
AAGAGAGCGACGGCCTGCGCTTCTATCGCACCCCTGGCGCAGCCGGGAACCTGCCGCTTGTACGGGAATGGGCCGAAGTG
TCGGCCCTTGCCCGGCGCATCGTCGAGGTCGCCGGTGAATGGCGACCCGATATCCTCCACGCGCACTCGCCCGCCTTGTG
CGGCCTCGCGGCGGTAAAGGCCGGGCGCAAGCTCGGCATTCCGGTGGTCTACGAAATCCGCGCCTTCTGGGAGGATGCGG
CCGTCGGCAACGGGACAGGCCGCGAAGGCAGCCTCAAGTACCGCGTCACGCGGGCGATGGAGAACGACGTCGTCGGTGCC
GCCGCCCGTGTCGTCACGATCTGCGAGGGTCTTCGCCAGGACCTGGTCGGACGCGGATTCGCGCCCGAGAAGCTGTCGAT
CATGCCCAACGGCGTCGACCTCGATCTGTTCGGCGCGCCCCTGCCGCGCGACCTTGGCCTCGCGCAGGAGCTTGGTCTCG
GCGACGGACCCGTCATCGGCTTCCTGGGCAGCTTTTATCCCTACGAGGGGCTGGACGATCTTGTCGACGCAATGCCGGCG
ATCGCCGGCGCGGTGCCTGGCGCCACGCTCCTGCTCGTCGGCGGAGGGCCGGCGGAAGCAGACCTTCGTGCCCGCGCCGC
CGCTTCGCCGGCGGCCCCGGCGATCCGTTTCGTCGGCCGCGTGCCTCATCACGAGGTGGACCGCTATTATTCGCTGGTCG
ATGTCGTCTGCTATCCGCGCAAGGCCATGCGCCTTACCGAAATGGTGACCCCGCTCAAGCCGCTTGAGGCGATGGCTCAG
GGCAAGCTCGTGGCGGCGTCCGACGTCGGCGGGCACCGCGAACTTGTCACCGATGGCGAGACCGGGGCGCTGTTCCCCCC
GGACGACCCTGCGGGTCTCGCCGCCGCGCTTGTTTCGCTGCTCGCCGGGCGCGACGGCTGGGAGGAAAGGCGTGCGACGG
CGAGGGCGTTCGTCCGGGACCGGCACGATTGGGCGATCAATGTGCGGCGTTATCAGGACGTTTACCAAGCCTTGTTACCG
AGTCCTTGA

Upstream 100 bases:

>100_bases
ACGTGCGGCAGGAATATCGTGAACTTGTCGATCATCTGGCCTGACGGCTAGCGCATCGAGGTTGACGCTGCGTTAAGCCT
TCCGTGGCACGGGTGTGGCC

Downstream 100 bases:

>100_bases
AAGACGGTGGCGAGAACCACGTGTTCGACTTTCCCGAATGCAAAACAGGCCCGGGCATAGGCGGGCCGGAACGTAGACAG
GACGGACCGAAGTGACTGCG

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: Alpha-mannosyltransferase; Guanosine diphosphomannose-phosphatidyl-inositol alpha-mannosyltransferase; Phosphatidylinositol alpha-mannosyltransferase; PI alpha-mannosyltransferase [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTRVLHVLDHSLPLHSGYTFRTRAILKAQEAMGIEVRGVTGRRHVAPPAAQEPEESDGLRFYRTPGAAGNLPLVREWAEV
SALARRIVEVAGEWRPDILHAHSPALCGLAAVKAGRKLGIPVVYEIRAFWEDAAVGNGTGREGSLKYRVTRAMENDVVGA
AARVVTICEGLRQDLVGRGFAPEKLSIMPNGVDLDLFGAPLPRDLGLAQELGLGDGPVIGFLGSFYPYEGLDDLVDAMPA
IAGAVPGATLLLVGGGPAEADLRARAAASPAAPAIRFVGRVPHHEVDRYYSLVDVVCYPRKAMRLTEMVTPLKPLEAMAQ
GKLVAASDVGGHRELVTDGETGALFPPDDPAGLAAALVSLLAGRDGWEERRATARAFVRDRHDWAINVRRYQDVYQALLP
SP

Sequences:

>Translated_402_residues
MTRVLHVLDHSLPLHSGYTFRTRAILKAQEAMGIEVRGVTGRRHVAPPAAQEPEESDGLRFYRTPGAAGNLPLVREWAEV
SALARRIVEVAGEWRPDILHAHSPALCGLAAVKAGRKLGIPVVYEIRAFWEDAAVGNGTGREGSLKYRVTRAMENDVVGA
AARVVTICEGLRQDLVGRGFAPEKLSIMPNGVDLDLFGAPLPRDLGLAQELGLGDGPVIGFLGSFYPYEGLDDLVDAMPA
IAGAVPGATLLLVGGGPAEADLRARAAASPAAPAIRFVGRVPHHEVDRYYSLVDVVCYPRKAMRLTEMVTPLKPLEAMAQ
GKLVAASDVGGHRELVTDGETGALFPPDDPAGLAAALVSLLAGRDGWEERRATARAFVRDRHDWAINVRRYQDVYQALLP
SP
>Mature_401_residues
TRVLHVLDHSLPLHSGYTFRTRAILKAQEAMGIEVRGVTGRRHVAPPAAQEPEESDGLRFYRTPGAAGNLPLVREWAEVS
ALARRIVEVAGEWRPDILHAHSPALCGLAAVKAGRKLGIPVVYEIRAFWEDAAVGNGTGREGSLKYRVTRAMENDVVGAA
ARVVTICEGLRQDLVGRGFAPEKLSIMPNGVDLDLFGAPLPRDLGLAQELGLGDGPVIGFLGSFYPYEGLDDLVDAMPAI
AGAVPGATLLLVGGGPAEADLRARAAASPAAPAIRFVGRVPHHEVDRYYSLVDVVCYPRKAMRLTEMVTPLKPLEAMAQG
KLVAASDVGGHRELVTDGETGALFPPDDPAGLAAALVSLLAGRDGWEERRATARAFVRDRHDWAINVRRYQDVYQALLPS
P

Specific function: Catalyzes the addition of a mannose residue from GDP-D- mannose to the position 2 of a phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: =2.4.1.57 [H]

Molecular weight: Translated: 43124; Mature: 42993

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVLHVLDHSLPLHSGYTFRTRAILKAQEAMGIEVRGVTGRRHVAPPAAQEPEESDGLR
CCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCEE
FYRTPGAAGNLPLVREWAEVSALARRIVEVAGEWRPDILHAHSPALCGLAAVKAGRKLGI
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHCCCCCCC
PVVYEIRAFWEDAAVGNGTGREGSLKYRVTRAMENDVVGAAARVVTICEGLRQDLVGRGF
CCEEHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
APEKLSIMPNGVDLDLFGAPLPRDLGLAQELGLGDGPVIGFLGSFYPYEGLDDLVDAMPA
CCCCEEECCCCCEEEECCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHH
IAGAVPGATLLLVGGGPAEADLRARAAASPAAPAIRFVGRVPHHEVDRYYSLVDVVCYPR
HHHCCCCCEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCH
KAMRLTEMVTPLKPLEAMAQGKLVAASDVGGHRELVTDGETGALFPPDDPAGLAAALVSL
HHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
LAGRDGWEERRATARAFVRDRHDWAINVRRYQDVYQALLPSP
HHCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRVLHVLDHSLPLHSGYTFRTRAILKAQEAMGIEVRGVTGRRHVAPPAAQEPEESDGLR
CHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCEE
FYRTPGAAGNLPLVREWAEVSALARRIVEVAGEWRPDILHAHSPALCGLAAVKAGRKLGI
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHCCCCCCC
PVVYEIRAFWEDAAVGNGTGREGSLKYRVTRAMENDVVGAAARVVTICEGLRQDLVGRGF
CCEEHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
APEKLSIMPNGVDLDLFGAPLPRDLGLAQELGLGDGPVIGFLGSFYPYEGLDDLVDAMPA
CCCCEEECCCCCEEEECCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHH
IAGAVPGATLLLVGGGPAEADLRARAAASPAAPAIRFVGRVPHHEVDRYYSLVDVVCYPR
HHHCCCCCEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCH
KAMRLTEMVTPLKPLEAMAQGKLVAASDVGGHRELVTDGETGALFPPDDPAGLAAALVSL
HHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
LAGRDGWEERRATARAFVRDRHDWAINVRRYQDVYQALLPSP
HHCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]