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The map label for this gene is gltB [H]

Identifier: 87201187

GI number: 87201187

Start: 3390562

End: 3391995

Strand: Reverse

Name: gltB [H]

Synonym: Saro_3175

Alternate gene names: 87201187

Gene position: 3391995-3390562 (Counterclockwise)

Preceding gene: 87201188

Following gene: 87201185

Centisome position: 95.24

GC content: 65.97

Gene sequence:

>1434_bases
ATGGGCAAGGCAACCGGCTTTCTCGAAATCGACCGCAAGGACCGCACCTATGATGCGCCTGCGGAACGTCTGAAGCACTA
TCGCGAGTTCGTGGTCCCGCACGATGAGGCAGGCCTCAAGGGGCAGGCCGCGCGGTGCATGAACTGCGGCATTCCGTACT
GCCACAACGGCTGTCCGGTGAACAACCAGATCCCCGACTGGAACCACCTGGTCTACGAGAACGACTGGCGCGACGCGCTG
ACCAATCTCCACTCGACCAACAACTTCCCCGAGTTCACCGGTCGTATCTGCCCCGCTCCCTGCGAGGCGGCCTGCACGCT
GAACATCATCGACCAGCCGGTCACGATCAAGTCGATCGAATGCGCGATCGTCGACCGCGGGTGGAAGGAAGGCTGGATCG
AGCCGCAGGTTCCCGTGAAGAAGACCGGCAAGTCGGTTGCGGTCGTCGGGTCGGGCCCCGCGGGCCTTGCCGCCGCGCAG
CAGCTTGCGCGCGCCGGCCACTCGGTGACGGTGTTCGAGAAGAACGACCGCGTCGGCGGCCTGCTTCGCTATGGCATCCC
CGACTTCAAGATGGAAAAGACCCACATCAATCGCCGCGCGATGCAGATGGAGGCCGAAGGCGTCCAGTTCCGCACCGGCG
TCGAAGTGGGCGTGACCGTCTCGTTCGCCTCGCTGCAGGAGAACTTCGACGCGGTGGTGCTGTCGGGCGGTGCGGAAGAT
CCGCGCGGCCTCCAGATCCCGGGCGCCGAGCTGCCGAGCGTGCGCATGGCGATGGAATTCCTGACCCAGCAGAACAAGCG
CAACGCCGGCGACGACGAACTGCGCGCCGCTCCGCGCGGTTCGCTCTTCGCGACTGGCAAGCATGTCGTCGTGATCGGCG
GCGGCGATACAGGTTCGGACTGCGTCGGCACCTCGAACCGTCAGGGCGCGCTCTCGGTTACCCAGCTCGAGATCATGCCC
AAGCCGCCCGAAAAGGAAGACAAGGCGCTGTCCTGGCCGAACTGGCCGCTCAAGCTGCGCACCTCGTCGAGCCACGAGGA
AGGCTGCGAACGCGAGTTCGCCGTGCTAACCAAGCGCGTGGTGGGCGAGAACGATGTCACCGGCCTCGAGTGCGTCCGCG
TCGAGTGGGTCAATGGCCAGATGCAGGAAGTGCCCGGCAGCGAATTCGTGCTCAAGGCCGACCTCATCCTGCTTGCCATG
GGCTTCGTCGGCCCGCGCAAGCAGGGCCTGCTCGAACAGGCCGGCGTCGAGCTTGACGCGCGCGGCAACGTCAAGGGCAA
CGTCGTCGACTACAAGACCAGCGTCGAGAACGTGTTCGCCTGCGGCGACATGCGTCGCGGTCAGAGCCTGGTGGTCTGGG
CGATCCGCGAAGGCCGCCAGTGCGCGCGCGCCGTCGACGAAGCGCTCATGGGCGTCAGCGAACTGCCGCGCTGA

Upstream 100 bases:

>100_bases
CGCGCGATTATGCGCGTGCGCTCCGCCAGATGGAGGCAGAGCGCCTCGAAGCCGCCTCCGTGGCGGCCGAGTAAATTCGG
GGAATAAAGGTTAGATTACA

Downstream 100 bases:

>100_bases
CCGTCGCGAAATCGACCGAACAACGAAAAGGGCGCCCGCGACGGCGCCCTTTCTCGTTGGCTCGTACCGGGTGGGAAAGG
CCGTCCGGCTGACGGTCTCA

Product: glutamate synthase (NADH) small subunit

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MGKATGFLEIDRKDRTYDAPAERLKHYREFVVPHDEAGLKGQAARCMNCGIPYCHNGCPVNNQIPDWNHLVYENDWRDAL
TNLHSTNNFPEFTGRICPAPCEAACTLNIIDQPVTIKSIECAIVDRGWKEGWIEPQVPVKKTGKSVAVVGSGPAGLAAAQ
QLARAGHSVTVFEKNDRVGGLLRYGIPDFKMEKTHINRRAMQMEAEGVQFRTGVEVGVTVSFASLQENFDAVVLSGGAED
PRGLQIPGAELPSVRMAMEFLTQQNKRNAGDDELRAAPRGSLFATGKHVVVIGGGDTGSDCVGTSNRQGALSVTQLEIMP
KPPEKEDKALSWPNWPLKLRTSSSHEEGCEREFAVLTKRVVGENDVTGLECVRVEWVNGQMQEVPGSEFVLKADLILLAM
GFVGPRKQGLLEQAGVELDARGNVKGNVVDYKTSVENVFACGDMRRGQSLVVWAIREGRQCARAVDEALMGVSELPR

Sequences:

>Translated_477_residues
MGKATGFLEIDRKDRTYDAPAERLKHYREFVVPHDEAGLKGQAARCMNCGIPYCHNGCPVNNQIPDWNHLVYENDWRDAL
TNLHSTNNFPEFTGRICPAPCEAACTLNIIDQPVTIKSIECAIVDRGWKEGWIEPQVPVKKTGKSVAVVGSGPAGLAAAQ
QLARAGHSVTVFEKNDRVGGLLRYGIPDFKMEKTHINRRAMQMEAEGVQFRTGVEVGVTVSFASLQENFDAVVLSGGAED
PRGLQIPGAELPSVRMAMEFLTQQNKRNAGDDELRAAPRGSLFATGKHVVVIGGGDTGSDCVGTSNRQGALSVTQLEIMP
KPPEKEDKALSWPNWPLKLRTSSSHEEGCEREFAVLTKRVVGENDVTGLECVRVEWVNGQMQEVPGSEFVLKADLILLAM
GFVGPRKQGLLEQAGVELDARGNVKGNVVDYKTSVENVFACGDMRRGQSLVVWAIREGRQCARAVDEALMGVSELPR
>Mature_476_residues
GKATGFLEIDRKDRTYDAPAERLKHYREFVVPHDEAGLKGQAARCMNCGIPYCHNGCPVNNQIPDWNHLVYENDWRDALT
NLHSTNNFPEFTGRICPAPCEAACTLNIIDQPVTIKSIECAIVDRGWKEGWIEPQVPVKKTGKSVAVVGSGPAGLAAAQQ
LARAGHSVTVFEKNDRVGGLLRYGIPDFKMEKTHINRRAMQMEAEGVQFRTGVEVGVTVSFASLQENFDAVVLSGGAEDP
RGLQIPGAELPSVRMAMEFLTQQNKRNAGDDELRAAPRGSLFATGKHVVVIGGGDTGSDCVGTSNRQGALSVTQLEIMPK
PPEKEDKALSWPNWPLKLRTSSSHEEGCEREFAVLTKRVVGENDVTGLECVRVEWVNGQMQEVPGSEFVLKADLILLAMG
FVGPRKQGLLEQAGVELDARGNVKGNVVDYKTSVENVFACGDMRRGQSLVVWAIREGRQCARAVDEALMGVSELPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=466, Percent_Identity=25.1072961373391, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI111118983, Length=122, Percent_Identity=42.6229508196721, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI111118981, Length=122, Percent_Identity=42.6229508196721, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1789606, Length=473, Percent_Identity=38.0549682875264, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI1788811, Length=454, Percent_Identity=36.784140969163, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI87082180, Length=466, Percent_Identity=34.1201716738197, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1788468, Length=433, Percent_Identity=28.175519630485, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1789243, Length=435, Percent_Identity=29.1954022988506, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17570289, Length=495, Percent_Identity=45.2525252525253, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71984108, Length=451, Percent_Identity=23.9467849223947, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17543860, Length=185, Percent_Identity=33.5135135135135, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320030, Length=494, Percent_Identity=46.1538461538462, Blast_Score=429, Evalue=1e-121,
Organism=Drosophila melanogaster, GI28574881, Length=488, Percent_Identity=50.2049180327869, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24665539, Length=488, Percent_Identity=50.2049180327869, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24665547, Length=488, Percent_Identity=50.2049180327869, Blast_Score=470, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24665543, Length=488, Percent_Identity=50.2049180327869, Blast_Score=470, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24640763, Length=472, Percent_Identity=25.8474576271186, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI18858217, Length=472, Percent_Identity=25.8474576271186, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52108; Mature: 51977

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKATGFLEIDRKDRTYDAPAERLKHYREFVVPHDEAGLKGQAARCMNCGIPYCHNGCPV
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCC
NNQIPDWNHLVYENDWRDALTNLHSTNNFPEFTGRICPAPCEAACTLNIIDQPVTIKSIE
CCCCCCCCCEEEECCHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEEEECCCCEEEEEEE
CAIVDRGWKEGWIEPQVPVKKTGKSVAVVGSGPAGLAAAQQLARAGHSVTVFEKNDRVGG
EEEEECCCCCCCCCCCCCHHHCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCC
LLRYGIPDFKMEKTHINRRAMQMEAEGVQFRTGVEVGVTVSFASLQENFDAVVLSGGAED
EEECCCCCCEEHHHHHHHHHHHHHCCCEEEECCCEEEEEEEEHHHHCCCCEEEEECCCCC
PRGLQIPGAELPSVRMAMEFLTQQNKRNAGDDELRAAPRGSLFATGKHVVVIGGGDTGSD
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEECCCEEEEEECCCCCCC
CVGTSNRQGALSVTQLEIMPKPPEKEDKALSWPNWPLKLRTSSSHEEGCEREFAVLTKRV
CCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
VGENDVTGLECVRVEWVNGQMQEVPGSEFVLKADLILLAMGFVGPRKQGLLEQAGVELDA
CCCCCCCCEEEEEEEEECCHHHHCCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCEEEC
RGNVKGNVVDYKTSVENVFACGDMRRGQSLVVWAIREGRQCARAVDEALMGVSELPR
CCCCCCCEEEEECCHHHHEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKATGFLEIDRKDRTYDAPAERLKHYREFVVPHDEAGLKGQAARCMNCGIPYCHNGCPV
CCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCC
NNQIPDWNHLVYENDWRDALTNLHSTNNFPEFTGRICPAPCEAACTLNIIDQPVTIKSIE
CCCCCCCCCEEEECCHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEEEECCCCEEEEEEE
CAIVDRGWKEGWIEPQVPVKKTGKSVAVVGSGPAGLAAAQQLARAGHSVTVFEKNDRVGG
EEEEECCCCCCCCCCCCCHHHCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCC
LLRYGIPDFKMEKTHINRRAMQMEAEGVQFRTGVEVGVTVSFASLQENFDAVVLSGGAED
EEECCCCCCEEHHHHHHHHHHHHHCCCEEEECCCEEEEEEEEHHHHCCCCEEEEECCCCC
PRGLQIPGAELPSVRMAMEFLTQQNKRNAGDDELRAAPRGSLFATGKHVVVIGGGDTGSD
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEECCCEEEEEECCCCCCC
CVGTSNRQGALSVTQLEIMPKPPEKEDKALSWPNWPLKLRTSSSHEEGCEREFAVLTKRV
CCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
VGENDVTGLECVRVEWVNGQMQEVPGSEFVLKADLILLAMGFVGPRKQGLLEQAGVELDA
CCCCCCCCEEEEEEEEECCHHHHCCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCEEEC
RGNVKGNVVDYKTSVENVFACGDMRRGQSLVVWAIREGRQCARAVDEALMGVSELPR
CCCCCCCEEEEECCHHHHEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]