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The map label for this gene is rsmB [H]

Identifier: 87201108

GI number: 87201108

Start: 3315639

End: 3316898

Strand: Reverse

Name: rsmB [H]

Synonym: Saro_3096

Alternate gene names: 87201108

Gene position: 3316898-3315639 (Counterclockwise)

Preceding gene: 87201110

Following gene: 87201106

Centisome position: 93.13

GC content: 73.02

Gene sequence:

>1260_bases
ATGGCCCGTTCCAACACACCCGACGACATTCCCGGCGTCGCATCCCGCCGTGCCGCCCTTCGCCTGCTCGATGCGGTGCT
GCGCCGGGGCGATCCGCTCGACCAGGCCGCGCCGCCGGTCTTGCGCGGGATCGAGCGCGGCGCCGACCGCGCCCTTGCCC
TCGCAATTGCCCAGGAAGCGCTGCGCTGGATGGCCGACCTCGACGCGCTGATCGACGGCGCGACGCGGCAGATCCTGCCC
GAGGACGCAAAGGCGCGCAGCGTGCTTCGCATCATGCTGGCGCAGGCGCTGCGGCTCGATATGCCGGGGCACGCGGTGGT
GGCGACCGGCCTGCCGCTGCTCATGGGCGGGCCGCGCCGGCTTGCGCATGGCGTGTTCTCGGCGGTGACCAAGGGCGATG
CCAGGCTGCCCGAGGCGCCCACGCTGCCGCCGGCCGTCGCGGCACGCTGGCAGGCCGCCTGGCCGGACCGCGTTGACGGC
ATCGCGCGCGGTCTTGCCGAACCGCCGCCGCTCGACCTTACGCTGCGAGACCCGGCCGAAACCGCGCAGTGGGCGGAGCG
GCTCGGCGGCATGTCGCTTGCCCCGGGGCATGTGCGCATCGCCCGCGCTGGCGCGATCGAGGCGCTGCCGGGGTTCGCAG
AGGGCGCTTGGTGGGTGCAGGACCTTGCCGCGAGCGTCCCCGCCCGTCTGCTTGGCGCGGGAGAGGGGCGCCGTGCGCTC
GACCTCTGTGCCGCGCCGGGCGGGAAAACCCTGCAACTTGCTGCCGCTGGCTGGTCGGTGACCGCGCTCGACAAGTCCGC
GCGCCGGGTGGAGCGCCTCGAGGACAACCTGAAGCGCAGCCAGCTCGAGGCCGAGGTGGTGGTCGCCGATGCGCTGAAGT
GGCAGCCCGAGGTGAAGTTCGACGCCATTCTGCTCGATGCGCCGTGCAGCGCCACCGGGACCTGCCGCCGTCATCCCGAC
GTGCTGCACCGCAATCCCGGAATCGACGAGCTTGTCGCCTTGCAGAAGGCGATGCTCGAACGCGCGGCAGGCTGGCTCGC
GCCGGGCGGGCGGCTGGTCTATGCGGTCTGCTCGCTCGAGCCGGAGGAAGGCGAGGCGCAGGCCGCCGCCTTCACCGGCC
TCGCTCCCCTGCCGATAGGCGACGACGAACTGCCCGGGGGTTTGAAGGCGGCGGACGGCTGGTTCCGTTCCGACCCCGGG
ATGCTTGCCGATGCGGGCGGCATCGACGGTTTCTTCGCCGCCCGCTGGGTGAAGGGCTAG

Upstream 100 bases:

>100_bases
CCGGGTCGCGCGGGGAGTGAGCTTGTCCATGCCAGTGCAGATGGGCTTTCCCGATGCGAAGCGCAATTGCCATTTGGGGC
AGCGCAGGCGATAGGCGCGC

Downstream 100 bases:

>100_bases
GCCTTCACCTTGTTGGCGAAGCTCTTGCGCAACTTCATCAGCTTGGGCGGGATCACCGCCAGGCAGTAGGGGTTCCGCTG
GCCTTCGCCTTCCCAGTATT

Product: Fmu (Sun)

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MARSNTPDDIPGVASRRAALRLLDAVLRRGDPLDQAAPPVLRGIERGADRALALAIAQEALRWMADLDALIDGATRQILP
EDAKARSVLRIMLAQALRLDMPGHAVVATGLPLLMGGPRRLAHGVFSAVTKGDARLPEAPTLPPAVAARWQAAWPDRVDG
IARGLAEPPPLDLTLRDPAETAQWAERLGGMSLAPGHVRIARAGAIEALPGFAEGAWWVQDLAASVPARLLGAGEGRRAL
DLCAAPGGKTLQLAAAGWSVTALDKSARRVERLEDNLKRSQLEAEVVVADALKWQPEVKFDAILLDAPCSATGTCRRHPD
VLHRNPGIDELVALQKAMLERAAGWLAPGGRLVYAVCSLEPEEGEAQAAAFTGLAPLPIGDDELPGGLKAADGWFRSDPG
MLADAGGIDGFFAARWVKG

Sequences:

>Translated_419_residues
MARSNTPDDIPGVASRRAALRLLDAVLRRGDPLDQAAPPVLRGIERGADRALALAIAQEALRWMADLDALIDGATRQILP
EDAKARSVLRIMLAQALRLDMPGHAVVATGLPLLMGGPRRLAHGVFSAVTKGDARLPEAPTLPPAVAARWQAAWPDRVDG
IARGLAEPPPLDLTLRDPAETAQWAERLGGMSLAPGHVRIARAGAIEALPGFAEGAWWVQDLAASVPARLLGAGEGRRAL
DLCAAPGGKTLQLAAAGWSVTALDKSARRVERLEDNLKRSQLEAEVVVADALKWQPEVKFDAILLDAPCSATGTCRRHPD
VLHRNPGIDELVALQKAMLERAAGWLAPGGRLVYAVCSLEPEEGEAQAAAFTGLAPLPIGDDELPGGLKAADGWFRSDPG
MLADAGGIDGFFAARWVKG
>Mature_418_residues
ARSNTPDDIPGVASRRAALRLLDAVLRRGDPLDQAAPPVLRGIERGADRALALAIAQEALRWMADLDALIDGATRQILPE
DAKARSVLRIMLAQALRLDMPGHAVVATGLPLLMGGPRRLAHGVFSAVTKGDARLPEAPTLPPAVAARWQAAWPDRVDGI
ARGLAEPPPLDLTLRDPAETAQWAERLGGMSLAPGHVRIARAGAIEALPGFAEGAWWVQDLAASVPARLLGAGEGRRALD
LCAAPGGKTLQLAAAGWSVTALDKSARRVERLEDNLKRSQLEAEVVVADALKWQPEVKFDAILLDAPCSATGTCRRHPDV
LHRNPGIDELVALQKAMLERAAGWLAPGGRLVYAVCSLEPEEGEAQAAAFTGLAPLPIGDDELPGGLKAADGWFRSDPGM
LADAGGIDGFFAARWVKG

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=232, Percent_Identity=30.6034482758621, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI76150623, Length=232, Percent_Identity=30.6034482758621, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI301336155, Length=194, Percent_Identity=32.4742268041237, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI39995082, Length=194, Percent_Identity=32.4742268041237, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI32698918, Length=174, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI2367212, Length=436, Percent_Identity=33.4862385321101, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI87081985, Length=226, Percent_Identity=34.9557522123894, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17536757, Length=239, Percent_Identity=30.1255230125523, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25143471, Length=178, Percent_Identity=31.4606741573034, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319447, Length=228, Percent_Identity=32.8947368421053, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324268, Length=233, Percent_Identity=30.0429184549356, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI22024126, Length=230, Percent_Identity=28.2608695652174, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24668781, Length=176, Percent_Identity=35.2272727272727, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356513, Length=176, Percent_Identity=35.2272727272727, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 44236; Mature: 44105

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSNTPDDIPGVASRRAALRLLDAVLRRGDPLDQAAPPVLRGIERGADRALALAIAQEA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHCHHHHHHHHHHHHH
LRWMADLDALIDGATRQILPEDAKARSVLRIMLAQALRLDMPGHAVVATGLPLLMGGPRR
HHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHCCCHHHCCCHHH
LAHGVFSAVTKGDARLPEAPTLPPAVAARWQAAWPDRVDGIARGLAEPPPLDLTLRDPAE
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEECCHHH
TAQWAERLGGMSLAPGHVRIARAGAIEALPGFAEGAWWVQDLAASVPARLLGAGEGRRAL
HHHHHHHHCCCCCCCCCEEEEECCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
DLCAAPGGKTLQLAAAGWSVTALDKSARRVERLEDNLKRSQLEAEVVVADALKWQPEVKF
HHHCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
DAILLDAPCSATGTCRRHPDVLHRNPGIDELVALQKAMLERAAGWLAPGGRLVYAVCSLE
EEEEEECCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECC
PEEGEAQAAAFTGLAPLPIGDDELPGGLKAADGWFRSDPGMLADAGGIDGFFAARWVKG
CCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHCCC
>Mature Secondary Structure 
ARSNTPDDIPGVASRRAALRLLDAVLRRGDPLDQAAPPVLRGIERGADRALALAIAQEA
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHCHHHHHHHHHHHHH
LRWMADLDALIDGATRQILPEDAKARSVLRIMLAQALRLDMPGHAVVATGLPLLMGGPRR
HHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHCCCHHHCCCHHH
LAHGVFSAVTKGDARLPEAPTLPPAVAARWQAAWPDRVDGIARGLAEPPPLDLTLRDPAE
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEECCHHH
TAQWAERLGGMSLAPGHVRIARAGAIEALPGFAEGAWWVQDLAASVPARLLGAGEGRRAL
HHHHHHHHCCCCCCCCCEEEEECCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
DLCAAPGGKTLQLAAAGWSVTALDKSARRVERLEDNLKRSQLEAEVVVADALKWQPEVKF
HHHCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
DAILLDAPCSATGTCRRHPDVLHRNPGIDELVALQKAMLERAAGWLAPGGRLVYAVCSLE
EEEEEECCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECC
PEEGEAQAAAFTGLAPLPIGDDELPGGLKAADGWFRSDPGMLADAGGIDGFFAARWVKG
CCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA