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The map label for this gene is sppA [H]

Identifier: 87201072

GI number: 87201072

Start: 3277719

End: 3279596

Strand: Reverse

Name: sppA [H]

Synonym: Saro_3060

Alternate gene names: 87201072

Gene position: 3279596-3277719 (Counterclockwise)

Preceding gene: 87201075

Following gene: 87201071

Centisome position: 92.08

GC content: 67.2

Gene sequence:

>1878_bases
ATGAGCTTTGCCCGTTCCGTGTGGAAGATCCTCGTCGCCATCAAGGACGGTCTCGTCCTCCTGTTCCTGCTGCTGTTCTT
TGCGGGCCTATATGCCGTGCTCGCGATGCGCCCCGCGCCCGGCATGGTGCGCGAAGGGGCGCTCTACCTGCCGCTCGAAG
GCCGCGTAGTGGAAGAGGCGACCGAAATCTCGCCCGCCGACTTCCTGACCGGCCAGACCCCGGCGCCGGAATATGTCGAA
CGCGACCTGACCCGTGCGATCGAGGCTGCCGCCACGGACAAGCGCATCAAGGCCGTGGTACTCGATCTCGAATCCTTCGG
CGGCGGCTCTGCCGTCCATCTTTCAAGCATCGGCGCCGCGATGGACAGGGTCCGCGCCGCCAGGAAGCCGGTCTTCGTTC
GCTCGCTCATCTATACCGACGATGCGATGCAACTGGCCGCACATGCGTCGGAAGCGTGGGTCGATCCGATGGGCGGCGTC
GCGATCACCGGGCCGGGCGGCACGATGCTGTTCTATAAGGGGCTGATCGACAAGCTGAAGGCCAACGTCCACATCTTCAA
GGTCGGCACCTACAAGAGCGCGGTCGAACCCTACATGCGCGCGCAATCCTCGCCCGAGGCGCGCGAGGCGATGCAGGCGG
TCTATGGCGCGATCTGGCAGAACTGGCAGGACGAAGTGCGCAAGGCCCGGCCCAAGGCCGACCTTGCCCTCGCGACGACC
GACCCGGCGAAGTGGGTCGCAGACAATGGCGGCGATGCCGCGCAGGCCGCGCTGAAATCCGGCCTCATCGACCGCGTCGG
TGACCGCATCGCTTTCGGTAAGCGCGTGGCCGAGGTTGTCGGCGCGGACGATCAGGGACCGCTGGGTTCGTTCAAGGCGA
CCGAGCTTCCTGTCTATCTGGCCGACAAGCCCGCCGCCAAGGACGGCAGTGCTATCGCGGTGGTGACCGTGGCCGGCGAG
ATCGTCGATGGTGATGCCGGACCCGGCGTTGCGGGCGGCGACCGTATCGCGGACCTCATCGACGGCGTGACACAGGCCGA
CGACTACGCCGGCCTCGTGCTGCGCGTCGATTCGCCCGGCGGCTCGGTCATGGCCTCGGAACGCATCCGGGCGGCGGTGG
AGCGGGTCAGGGCCAAGGGCCTGCCGGTGGCGGTTTCGATGGGCAGCGTCGCGGCCAGCGGCGGCTACTGGGTTTCGACG
CCCGCGCAGCGCATCTTTGCAGAGCCCAGCACGATCACTGGTTCGATCGGCGTGTTCGCCGTCCTGCCGACGTTCGAGCA
GACCCTGCCGCAGTATGGGGTGACGACCGAACAGGTCCGCACCACCCCGCTTTCCGGCCAGCCCGATCTCCTCGGTGGTC
TCAACCCTCAGGTCTCGGCGCTCATGCAGGGGCAGGTCGAACAGACCTACACCCGCTTCCTCGGCCTCGTCGCCAAGGCG
CGCGGCAAATCCCCTGCCGACATCGACAGGATCGCGCAGGGACGCATCTGGGACGGCGGTACTGCACGCCAATTGGGCCT
GGTCGACCAGTTCGGCGGGCTGGACGACGCGGTCGCATGGGTCGCCAAGCAGGCCAAGGCCGACAAGTGGCACGCCGAAT
ACGTCGAGGACGAACCCAGCCCTGTCGCCCAGTTCCTCCGCCAGATGGAAACCGGCGAGGCACCCGAGGCAAGAGCGCAC
GATCTTGCAGGCGCGCTGGCAGCGCAGCAGCAGGGCCTTGTCGCGCGCATGCAGGCGGACCTGCTGCGGCTGATCGAAGG
CGGCGGGATCAAGGCTTATTGCCTGGAGTGTGCAAGCGATGATCGGGGCACCGCGAATATGCGGCAGCGGAAGGCCGATT
TGGGGCTTCTGGCCTGGCTCGGACGGCTGGTTGCCTGA

Upstream 100 bases:

>100_bases
TCTGGCTTTGCATTGAAACCAGTTCCCTAGTCGCGGGTTGAAGCGGGCTCAAGCGGTTTCCCGCTGGCCTTGGGGAGTCG
TCCACAATAGAGTCGAACCC

Downstream 100 bases:

>100_bases
GAGATCGGTTCGTCGAAACCACAACGCCGTCATCCTGAACTTGTTTCAGGATCCATCTCTCCCCACGAACCACAGGGTGG
GTTGAAAGTTGGACCCTGAA

Product: signal peptide peptidase SppA, 67K type

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MSFARSVWKILVAIKDGLVLLFLLLFFAGLYAVLAMRPAPGMVREGALYLPLEGRVVEEATEISPADFLTGQTPAPEYVE
RDLTRAIEAAATDKRIKAVVLDLESFGGGSAVHLSSIGAAMDRVRAARKPVFVRSLIYTDDAMQLAAHASEAWVDPMGGV
AITGPGGTMLFYKGLIDKLKANVHIFKVGTYKSAVEPYMRAQSSPEAREAMQAVYGAIWQNWQDEVRKARPKADLALATT
DPAKWVADNGGDAAQAALKSGLIDRVGDRIAFGKRVAEVVGADDQGPLGSFKATELPVYLADKPAAKDGSAIAVVTVAGE
IVDGDAGPGVAGGDRIADLIDGVTQADDYAGLVLRVDSPGGSVMASERIRAAVERVRAKGLPVAVSMGSVAASGGYWVST
PAQRIFAEPSTITGSIGVFAVLPTFEQTLPQYGVTTEQVRTTPLSGQPDLLGGLNPQVSALMQGQVEQTYTRFLGLVAKA
RGKSPADIDRIAQGRIWDGGTARQLGLVDQFGGLDDAVAWVAKQAKADKWHAEYVEDEPSPVAQFLRQMETGEAPEARAH
DLAGALAAQQQGLVARMQADLLRLIEGGGIKAYCLECASDDRGTANMRQRKADLGLLAWLGRLVA

Sequences:

>Translated_625_residues
MSFARSVWKILVAIKDGLVLLFLLLFFAGLYAVLAMRPAPGMVREGALYLPLEGRVVEEATEISPADFLTGQTPAPEYVE
RDLTRAIEAAATDKRIKAVVLDLESFGGGSAVHLSSIGAAMDRVRAARKPVFVRSLIYTDDAMQLAAHASEAWVDPMGGV
AITGPGGTMLFYKGLIDKLKANVHIFKVGTYKSAVEPYMRAQSSPEAREAMQAVYGAIWQNWQDEVRKARPKADLALATT
DPAKWVADNGGDAAQAALKSGLIDRVGDRIAFGKRVAEVVGADDQGPLGSFKATELPVYLADKPAAKDGSAIAVVTVAGE
IVDGDAGPGVAGGDRIADLIDGVTQADDYAGLVLRVDSPGGSVMASERIRAAVERVRAKGLPVAVSMGSVAASGGYWVST
PAQRIFAEPSTITGSIGVFAVLPTFEQTLPQYGVTTEQVRTTPLSGQPDLLGGLNPQVSALMQGQVEQTYTRFLGLVAKA
RGKSPADIDRIAQGRIWDGGTARQLGLVDQFGGLDDAVAWVAKQAKADKWHAEYVEDEPSPVAQFLRQMETGEAPEARAH
DLAGALAAQQQGLVARMQADLLRLIEGGGIKAYCLECASDDRGTANMRQRKADLGLLAWLGRLVA
>Mature_624_residues
SFARSVWKILVAIKDGLVLLFLLLFFAGLYAVLAMRPAPGMVREGALYLPLEGRVVEEATEISPADFLTGQTPAPEYVER
DLTRAIEAAATDKRIKAVVLDLESFGGGSAVHLSSIGAAMDRVRAARKPVFVRSLIYTDDAMQLAAHASEAWVDPMGGVA
ITGPGGTMLFYKGLIDKLKANVHIFKVGTYKSAVEPYMRAQSSPEAREAMQAVYGAIWQNWQDEVRKARPKADLALATTD
PAKWVADNGGDAAQAALKSGLIDRVGDRIAFGKRVAEVVGADDQGPLGSFKATELPVYLADKPAAKDGSAIAVVTVAGEI
VDGDAGPGVAGGDRIADLIDGVTQADDYAGLVLRVDSPGGSVMASERIRAAVERVRAKGLPVAVSMGSVAASGGYWVSTP
AQRIFAEPSTITGSIGVFAVLPTFEQTLPQYGVTTEQVRTTPLSGQPDLLGGLNPQVSALMQGQVEQTYTRFLGLVAKAR
GKSPADIDRIAQGRIWDGGTARQLGLVDQFGGLDDAVAWVAKQAKADKWHAEYVEDEPSPVAQFLRQMETGEAPEARAHD
LAGALAAQQQGLVARMQADLLRLIEGGGIKAYCLECASDDRGTANMRQRKADLGLLAWLGRLVA

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=612, Percent_Identity=35.9477124183007, Blast_Score=333, Evalue=3e-92,
Organism=Escherichia coli, GI1787527, Length=167, Percent_Identity=31.7365269461078, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.- [C]

Molecular weight: Translated: 66210; Mature: 66079

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFARSVWKILVAIKDGLVLLFLLLFFAGLYAVLAMRPAPGMVREGALYLPLEGRVVEEA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCEEEECCCCCHHHHH
TEISPADFLTGQTPAPEYVERDLTRAIEAAATDKRIKAVVLDLESFGGGSAVHLSSIGAA
HHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHCCCCCEEHHHHHHHH
MDRVRAARKPVFVRSLIYTDDAMQLAAHASEAWVDPMGGVAITGPGGTMLFYKGLIDKLK
HHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHH
ANVHIFKVGTYKSAVEPYMRAQSSPEAREAMQAVYGAIWQNWQDEVRKARPKADLALATT
CCEEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
DPAKWVADNGGDAAQAALKSGLIDRVGDRIAFGKRVAEVVGADDQGPLGSFKATELPVYL
CCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
ADKPAAKDGSAIAVVTVAGEIVDGDAGPGVAGGDRIADLIDGVTQADDYAGLVLRVDSPG
CCCCCCCCCCEEEEEEEECEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCC
GSVMASERIRAAVERVRAKGLPVAVSMGSVAASGGYWVSTPAQRIFAEPSTITGSIGVFA
CCHHHHHHHHHHHHHHHHCCCCEEEECCCEECCCCEEECCCHHHHHCCCCCCCCCCCCEE
VLPTFEQTLPQYGVTTEQVRTTPLSGQPDLLGGLNPQVSALMQGQVEQTYTRFLGLVAKA
EECCHHHHHHHCCCCHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RGKSPADIDRIAQGRIWDGGTARQLGLVDQFGGLDDAVAWVAKQAKADKWHAEYVEDEPS
CCCCCCCHHHHHCCCCCCCCCCHHHCCHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCCC
PVAQFLRQMETGEAPEARAHDLAGALAAQQQGLVARMQADLLRLIEGGGIKAYCLECASD
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEECCC
DRGTANMRQRKADLGLLAWLGRLVA
CCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SFARSVWKILVAIKDGLVLLFLLLFFAGLYAVLAMRPAPGMVREGALYLPLEGRVVEEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCEEEECCCCCHHHHH
TEISPADFLTGQTPAPEYVERDLTRAIEAAATDKRIKAVVLDLESFGGGSAVHLSSIGAA
HHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHCCCCCEEHHHHHHHH
MDRVRAARKPVFVRSLIYTDDAMQLAAHASEAWVDPMGGVAITGPGGTMLFYKGLIDKLK
HHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHH
ANVHIFKVGTYKSAVEPYMRAQSSPEAREAMQAVYGAIWQNWQDEVRKARPKADLALATT
CCEEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
DPAKWVADNGGDAAQAALKSGLIDRVGDRIAFGKRVAEVVGADDQGPLGSFKATELPVYL
CCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
ADKPAAKDGSAIAVVTVAGEIVDGDAGPGVAGGDRIADLIDGVTQADDYAGLVLRVDSPG
CCCCCCCCCCEEEEEEEECEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCC
GSVMASERIRAAVERVRAKGLPVAVSMGSVAASGGYWVSTPAQRIFAEPSTITGSIGVFA
CCHHHHHHHHHHHHHHHHCCCCEEEECCCEECCCCEEECCCHHHHHCCCCCCCCCCCCEE
VLPTFEQTLPQYGVTTEQVRTTPLSGQPDLLGGLNPQVSALMQGQVEQTYTRFLGLVAKA
EECCHHHHHHHCCCCHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RGKSPADIDRIAQGRIWDGGTARQLGLVDQFGGLDDAVAWVAKQAKADKWHAEYVEDEPS
CCCCCCCHHHHHCCCCCCCCCCHHHCCHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCCC
PVAQFLRQMETGEAPEARAHDLAGALAAQQQGLVARMQADLLRLIEGGGIKAYCLECASD
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEECCC
DRGTANMRQRKADLGLLAWLGRLVA
CCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]