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The map label for this gene is tig

Identifier: 87201071

GI number: 87201071

Start: 3275439

End: 3276980

Strand: Reverse

Name: tig

Synonym: Saro_3059

Alternate gene names: 87201071

Gene position: 3276980-3275439 (Counterclockwise)

Preceding gene: 87201072

Following gene: 87201070

Centisome position: 92.01

GC content: 64.72

Gene sequence:

>1542_bases
ATGCAGATCGTCGAAACCACCAACGAGGGCCTGAAGCGCGCCTACACTGTCACCATCCCCGCGGCCGACATCGCCGCCCG
GGTCGAGGGTGAAATCAAGAAGATCGCGCCGCAGGTGCGCATGCCCGGCTTCCGCCCCGGCAAGGTTCCCGCCAACCTCG
TCCGCAAGATGCACGGCCCGGCGCTCCACCAGGAAGCGCTGCAGACCACCATCCGCGAAGCGATGGACAAGCTGGTTGCC
GACAACAAGCTGCGTCCCGCGATGCAGCCTGCCGTCTCGCTCGGCGAAGGCTATGACGAAGGCAAGGACGCCGAGCTGAC
CGTCGAGCTCGAAGTCCTGCCCCAGATCGAAGCGCCTTCGCTTGATGGCTTGAAGCTCGAGAAGCTCGTCGTTCCCGTCA
CCGAAGCCGAAGTCGACGAAGCCGTCGCCCGCATCGGCCAGGGCCAGAAGTCCTTCACCGACGCCAAGAAGGGCGCGAAG
GCCAAGGACGGTGACCAGATCATCATCGACTTCCTCGGCAAGGTCGATGGCGTTGCCTTCGAAGGCGGCGCTGCCGAAGA
CACCCCGCTGGAACTCGGCGCGGGCCGCTTCATCCCCGGCTTCGAGGAACAGCTCGTCGGCGTGAAGGTCGGTGACGAGA
AGACCATCACCGTCACTTTCCCCGAAGACTATCCGGCCGAAAACCTCAAGGGCAAGGAAGCCACCTTCGACATCACCGTC
AAGGCGGTGAAGAACGCTGGTGAATTCGAAGCCAACGACGAATTCGCCAAGATGCTCGGCCTTGAAAGCCTCGAGCAGCT
CAAGACCCTGCTGCGCGGCCAGCTCGAGCAGGAAACTGCCGGCCTGACCCGCACCCAGATGAAGCGCGCCCTGCTTGACC
AGCTCGCCGCCGGCCACGACTTCGCCGTGCCGCCCTCGATGGTCGAAGCCGAGTTCGAGCAGATCTGGGCCCAGCTCCAG
CAGGAAGCCCAGCGCGAAGAGAACCCGGAAGAGGCCCTGAAGGAAATCGAGGCCGAGAAGGACGATTACCGCAAGATCGC
CGAACGTCGCGTGCGTCTCGGCCTGCTCCTGTCGGAAATCGGCCAGGCCAACGGGGTCCAGGTCACCAGCCAGGAAATGG
AAATGCTGATCCGCCAGGCGGCGCAGCAGTACCGCGAACAGGACCGCCAGCGCTTTGTCGACTACGTGCGTTCCGAACCC
CTTGCTGCCGCCCAGCTTCGCGCACCGCTCTACGAGGACAAGGTCGTCGACTTCCTGTTCGACAAGGCCGAAGTGACCGA
GCGTGAAGTGACCCGCGAGGAACTCCAGGCCGCGATCGAGGCCGAGGAAGGCGCCGCCGCCGAAGCCAAGCCCGCCAAGA
AGGCTCCGGCCAAGAAGAAGGCCGCCAAGGAAGAGGCTCCGGCCGAGGAAGCTGCCGCCGAGGAGAAGCCCAAGAAGGCC
GCTCCGAAGAAGAAGGCCGCTGCGGCTGACGAAGCCCCTGCCGCCGAGGAAGCTCCGGCTGCCAAGAAGAAGGCGCCGGC
CAAGAAAAAGGCCGAAGGCTGA

Upstream 100 bases:

>100_bases
GGGTTCGAGTCCCTTCGCCCGCACCAGTCCGCGCGCCGGCGTCCGAACAGCTTTTGCCGTGCCCAGTGGCGCGTGTGTGA
TACGAGAAAGTTGGATCAGG

Downstream 100 bases:

>100_bases
TCCATTACGGATTGATCCGGCCCGACGGGGCTGGATATCGGACGGCCCGGTGCGCTCAGGCGCGCCGGGCCGTTGCCGTT
GGAGAGATCGCCCTTCGACC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MQIVETTNEGLKRAYTVTIPAADIAARVEGEIKKIAPQVRMPGFRPGKVPANLVRKMHGPALHQEALQTTIREAMDKLVA
DNKLRPAMQPAVSLGEGYDEGKDAELTVELEVLPQIEAPSLDGLKLEKLVVPVTEAEVDEAVARIGQGQKSFTDAKKGAK
AKDGDQIIIDFLGKVDGVAFEGGAAEDTPLELGAGRFIPGFEEQLVGVKVGDEKTITVTFPEDYPAENLKGKEATFDITV
KAVKNAGEFEANDEFAKMLGLESLEQLKTLLRGQLEQETAGLTRTQMKRALLDQLAAGHDFAVPPSMVEAEFEQIWAQLQ
QEAQREENPEEALKEIEAEKDDYRKIAERRVRLGLLLSEIGQANGVQVTSQEMEMLIRQAAQQYREQDRQRFVDYVRSEP
LAAAQLRAPLYEDKVVDFLFDKAEVTEREVTREELQAAIEAEEGAAAEAKPAKKAPAKKKAAKEEAPAEEAAAEEKPKKA
APKKKAAAADEAPAAEEAPAAKKKAPAKKKAEG

Sequences:

>Translated_513_residues
MQIVETTNEGLKRAYTVTIPAADIAARVEGEIKKIAPQVRMPGFRPGKVPANLVRKMHGPALHQEALQTTIREAMDKLVA
DNKLRPAMQPAVSLGEGYDEGKDAELTVELEVLPQIEAPSLDGLKLEKLVVPVTEAEVDEAVARIGQGQKSFTDAKKGAK
AKDGDQIIIDFLGKVDGVAFEGGAAEDTPLELGAGRFIPGFEEQLVGVKVGDEKTITVTFPEDYPAENLKGKEATFDITV
KAVKNAGEFEANDEFAKMLGLESLEQLKTLLRGQLEQETAGLTRTQMKRALLDQLAAGHDFAVPPSMVEAEFEQIWAQLQ
QEAQREENPEEALKEIEAEKDDYRKIAERRVRLGLLLSEIGQANGVQVTSQEMEMLIRQAAQQYREQDRQRFVDYVRSEP
LAAAQLRAPLYEDKVVDFLFDKAEVTEREVTREELQAAIEAEEGAAAEAKPAKKAPAKKKAAKEEAPAEEAAAEEKPKKA
APKKKAAAADEAPAAEEAPAAKKKAPAKKKAEG
>Mature_513_residues
MQIVETTNEGLKRAYTVTIPAADIAARVEGEIKKIAPQVRMPGFRPGKVPANLVRKMHGPALHQEALQTTIREAMDKLVA
DNKLRPAMQPAVSLGEGYDEGKDAELTVELEVLPQIEAPSLDGLKLEKLVVPVTEAEVDEAVARIGQGQKSFTDAKKGAK
AKDGDQIIIDFLGKVDGVAFEGGAAEDTPLELGAGRFIPGFEEQLVGVKVGDEKTITVTFPEDYPAENLKGKEATFDITV
KAVKNAGEFEANDEFAKMLGLESLEQLKTLLRGQLEQETAGLTRTQMKRALLDQLAAGHDFAVPPSMVEAEFEQIWAQLQ
QEAQREENPEEALKEIEAEKDDYRKIAERRVRLGLLLSEIGQANGVQVTSQEMEMLIRQAAQQYREQDRQRFVDYVRSEP
LAAAQLRAPLYEDKVVDFLFDKAEVTEREVTREELQAAIEAEEGAAAEAKPAKKAPAKKKAAKEEAPAEEAAAEEKPKKA
APKKKAAAADEAPAAEEAPAAKKKAPAKKKAEG

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=442, Percent_Identity=35.2941176470588, Blast_Score=233, Evalue=2e-62,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_NOVAD (Q2G3S9)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_498328.1
- ProteinModelPortal:   Q2G3S9
- SMR:   Q2G3S9
- STRING:   Q2G3S9
- GeneID:   3916672
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_3059
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   Q2G3S9
- ProtClustDB:   PRK01490
- BioCyc:   NARO279238:SARO_3059-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PANTHER:   PTHR10516
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 55974; Mature: 55974

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIVETTNEGLKRAYTVTIPAADIAARVEGEIKKIAPQVRMPGFRPGKVPANLVRKMHGP
CCCCCCHHHHHHEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCH
ALHQEALQTTIREAMDKLVADNKLRPAMQPAVSLGEGYDEGKDAELTVELEVLPQIEAPS
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCC
LDGLKLEKLVVPVTEAEVDEAVARIGQGQKSFTDAKKGAKAKDGDQIIIDFLGKVDGVAF
CCCCCHHHEEECCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEE
EGGAAEDTPLELGAGRFIPGFEEQLVGVKVGDEKTITVTFPEDYPAENLKGKEATFDITV
CCCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCEEEEEECCCCCCCCCCCCCCCEEEEEH
KAVKNAGEFEANDEFAKMLGLESLEQLKTLLRGQLEQETAGLTRTQMKRALLDQLAAGHD
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCC
FAVPPSMVEAEFEQIWAQLQQEAQREENPEEALKEIEAEKDDYRKIAERRVRLGLLLSEI
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
GQANGVQVTSQEMEMLIRQAAQQYREQDRQRFVDYVRSEPLAAAQLRAPLYEDKVVDFLF
CCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHH
DKAEVTEREVTREELQAAIEAEEGAAAEAKPAKKAPAKKKAAKEEAPAEEAAAEEKPKKA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCHHC
APKKKAAAADEAPAAEEAPAAKKKAPAKKKAEG
CCHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure
MQIVETTNEGLKRAYTVTIPAADIAARVEGEIKKIAPQVRMPGFRPGKVPANLVRKMHGP
CCCCCCHHHHHHEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCH
ALHQEALQTTIREAMDKLVADNKLRPAMQPAVSLGEGYDEGKDAELTVELEVLPQIEAPS
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCC
LDGLKLEKLVVPVTEAEVDEAVARIGQGQKSFTDAKKGAKAKDGDQIIIDFLGKVDGVAF
CCCCCHHHEEECCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEE
EGGAAEDTPLELGAGRFIPGFEEQLVGVKVGDEKTITVTFPEDYPAENLKGKEATFDITV
CCCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCEEEEEECCCCCCCCCCCCCCCEEEEEH
KAVKNAGEFEANDEFAKMLGLESLEQLKTLLRGQLEQETAGLTRTQMKRALLDQLAAGHD
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCC
FAVPPSMVEAEFEQIWAQLQQEAQREENPEEALKEIEAEKDDYRKIAERRVRLGLLLSEI
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
GQANGVQVTSQEMEMLIRQAAQQYREQDRQRFVDYVRSEPLAAAQLRAPLYEDKVVDFLF
CCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHH
DKAEVTEREVTREELQAAIEAEEGAAAEAKPAKKAPAKKKAAKEEAPAEEAAAEEKPKKA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCHHC
APKKKAAAADEAPAAEEAPAAKKKAPAKKKAEG
CCHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA