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The map label for this gene is 87200959

Identifier: 87200959

GI number: 87200959

Start: 3165157

End: 3165831

Strand: Reverse

Name: 87200959

Synonym: Saro_2947

Alternate gene names: NA

Gene position: 3165831-3165157 (Counterclockwise)

Preceding gene: 87200960

Following gene: 87200958

Centisome position: 88.89

GC content: 64.0

Gene sequence:

>675_bases
ATGAGCACCGCCGTCTTCACACCCAAGTTCATCAGCTTCGACTGCTACGGCACGCTGACCCGCTTCCGCATGACCGAGAT
GGCGACGCAATTCTACGCCGACCGTCTTCCCGCCGAGGCGCTGCCTCGGTTCTGCAAGGACTGGTCCGCCTATCGCCTCG
ACGAAGTGCTGGGCGCCTGGAAGCCCTACCGCGAAGTGATCGCCAATTCGCTTGAACGCACCTCGAAGAAGTGGGGCGTG
GAGTTCCGCGAGGAAGAGGCCAGCGCGGTCTATGAAGCTGTGCCGACCTGGGGCCCGCACGAAGACGTTCCGGGCGGCCT
GTCGAAGATCTGCGACAAGATCCCGCTCGTCATCCTCTCCAACGCGATGAATTCGCAGATCATGTCGAATGTCGGCATGC
TCGGCGCGACGTTCCACCGCGTCTACACCGCCGAGAGCGCGCAGGCATACAAGCCGCGCATGCAGGCCTTCGAATACATG
TTCGACCAGCTGGGCATGGGTCCCGAGGTCGGCATGCACGTCTCGTCGAGCTTCCGCTACGACCAGAACACCGCGACCGA
CCTCGGCTTCGGCTGCCGCGTCTTCGTCGGCCGTGGCCACGAACCCTCGAACGCCAATTACCGCGACGTCGAGATCCCGC
ACATCGGCGCGCTGCCGGCGGTGGTCGGTCTCTGA

Upstream 100 bases:

>100_bases
AACGGATGCAAGTTCCGGCCCGGCGGTGGTGCTATCAATGACAGGAACCGCAGCCATCCGGCGTTGACCGGGGGCGACAG
ACAGGGATCAATGGCAACCC

Downstream 100 bases:

>100_bases
TCCGATGACGCCGCAGTCCCTCTCCTACTGGCAGGCCAGCACGCCGCCCTTCGCCTCGGGTGAGAAGGCGCGGGTCGAGG
GGGACTGCGACGTCGCGATC

Product: haloacid dehalogenase, type II

Products: (R)-2-hydroxyacid; halide

Alternate protein names: HAD Family Hydrolase; Hydrolase; Haloalkanoic Acid Dehalogenase; Dehalogenase; 2-Haloacid Dehalogenase; Haloacid Dehalogenase-Like Hydrolase; Haloacid Dehalogenase; Haloacid Dehalogenase I; 2-Haloalkanoic Acid Dehalogenase; Haloacid Dehydrogenase; 2-Haloacid Halidohydrolase Type II; Haloacid Dehalogenase Type II Truncated; 2-Haloacid Halidohydrolase IVa; 2-Haloacid Halidohydrolase IVA; Dehalogenase Protein; HAD Superfamily Haloalkanoic Acid Dehalogenase

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MSTAVFTPKFISFDCYGTLTRFRMTEMATQFYADRLPAEALPRFCKDWSAYRLDEVLGAWKPYREVIANSLERTSKKWGV
EFREEEASAVYEAVPTWGPHEDVPGGLSKICDKIPLVILSNAMNSQIMSNVGMLGATFHRVYTAESAQAYKPRMQAFEYM
FDQLGMGPEVGMHVSSSFRYDQNTATDLGFGCRVFVGRGHEPSNANYRDVEIPHIGALPAVVGL

Sequences:

>Translated_224_residues
MSTAVFTPKFISFDCYGTLTRFRMTEMATQFYADRLPAEALPRFCKDWSAYRLDEVLGAWKPYREVIANSLERTSKKWGV
EFREEEASAVYEAVPTWGPHEDVPGGLSKICDKIPLVILSNAMNSQIMSNVGMLGATFHRVYTAESAQAYKPRMQAFEYM
FDQLGMGPEVGMHVSSSFRYDQNTATDLGFGCRVFVGRGHEPSNANYRDVEIPHIGALPAVVGL
>Mature_223_residues
STAVFTPKFISFDCYGTLTRFRMTEMATQFYADRLPAEALPRFCKDWSAYRLDEVLGAWKPYREVIANSLERTSKKWGVE
FREEEASAVYEAVPTWGPHEDVPGGLSKICDKIPLVILSNAMNSQIMSNVGMLGATFHRVYTAESAQAYKPRMQAFEYMF
DQLGMGPEVGMHVSSSFRYDQNTATDLGFGCRVFVGRGHEPSNANYRDVEIPHIGALPAVVGL

Specific function: Unknown

COG id: COG1011

COG function: function code R; Predicted hydrolase (HAD superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.8.1.2

Molecular weight: Translated: 25134; Mature: 25003

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAVFTPKFISFDCYGTLTRFRMTEMATQFYADRLPAEALPRFCKDWSAYRLDEVLGAW
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
KPYREVIANSLERTSKKWGVEFREEEASAVYEAVPTWGPHEDVPGGLSKICDKIPLVILS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
NAMNSQIMSNVGMLGATFHRVYTAESAQAYKPRMQAFEYMFDQLGMGPEVGMHVSSSFRY
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
DQNTATDLGFGCRVFVGRGHEPSNANYRDVEIPHIGALPAVVGL
CCCCCCCCCCCEEEEEECCCCCCCCCCCEECCCCCCCHHHHHCC
>Mature Secondary Structure 
STAVFTPKFISFDCYGTLTRFRMTEMATQFYADRLPAEALPRFCKDWSAYRLDEVLGAW
CCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
KPYREVIANSLERTSKKWGVEFREEEASAVYEAVPTWGPHEDVPGGLSKICDKIPLVILS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
NAMNSQIMSNVGMLGATFHRVYTAESAQAYKPRMQAFEYMFDQLGMGPEVGMHVSSSFRY
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
DQNTATDLGFGCRVFVGRGHEPSNANYRDVEIPHIGALPAVVGL
CCCCCCCCCCCEEEEEECCCCCCCCCCCEECCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-haloacid; H2O

Specific reaction: (S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA