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The map label for this gene is metZ [H]

Identifier: 87200678

GI number: 87200678

Start: 2903330

End: 2904538

Strand: Direct

Name: metZ [H]

Synonym: Saro_2665

Alternate gene names: 87200678

Gene position: 2903330-2904538 (Clockwise)

Preceding gene: 87200676

Following gene: 87200679

Centisome position: 81.52

GC content: 66.25

Gene sequence:

>1209_bases
ATGAAACGCACGACTGGCCAGGACCGTTCGATCACCCGCAAGTGGCGCCCCGCAACCCAGGCGATTCGCGGGGGCACGTG
GCGCTCGGAGATGGGCGAGACATCCGAAGCGCTGTTCCTCACCTCGGGCTTCACGTACGACGACGCCGCCACCGTTGCGG
CGCGGTTCGCCGGCGAAGCGGAAGGCATGACCTACTCCCGCCTGCAGAATCCGACCGTGCAGATGCTGGAAGAACGCATC
GCCCTGCTCGAAGGCGCGGAAGCTTGCCGCACACAGGCAACGGGCATGGCCGCGATGACCACCGCCCTTCTCTGCCAGCT
TTCCGCGGGCGACCACATCGTCGCCGCCAAGGCCGCGTTCGGCTCGTGCCGCTGGCTCGTCGACAACCTGCTGCCCCGTT
TCGGCGTCCAGGGCACGACGATCGATGCCTCGGACAATGCCGCCTGGGAAGCGGCGATCCGGCCCAACACGAAGGTGTTC
TTCTTCGAAAGCCCGGCAAACCCGACGATGGACATCGTCGATCTCGAGTTCGTCTGCGGCCTCGCCAAAAAGCATGGCAT
CACCACCGTGGTCGACAATGCCTTCGCCACGGCCGCCCTCCAGCGCCCGATGGACTTCGGCGCCGACGTGGTTGCCTATT
CGGCTACCAAGATGATGGACGGCCAGGGCCGCGTCATGGCAGGCGCCGTCTGCGGCTCGGCCGACTGGATCAACAACGTG
CTGCTGCCGTTCCAGCGCAACACCGGCCCGAACATCGCCGCATTCAATGCCTGGGTCGTGCTCAAGGGCCTCGAAACGCT
CGACCTGCGCATCCACCGGCAGAGCGAGAACGCGCTGAAGGTCGCCAGCTTCGTCGAGAAGCGCGTGCCGCGCCTGCTCT
ATCCGGGCCTGCCGAGCCACCCCCAGCATGCCCTCGCGCAAAAGCAGATGAAGGCCGGCGGGACGATCTTCTCGTTCGAT
CTCGACGGTGGCCGGGCCCAGGCGCACGCACTGCTGGACGCGCTGCAACTGATCGACATCTCGAACAACATCGGCGACTC
GCGTTCGCTCATGTGCCACCCGGCCTCGACCACGCATCACGGCGTCGGGCCCGAGACTCGCGCGGACATGGGCGTTGGCG
AAGGCATGCTGCGCCTCAACGTGGGCCTGGAAGACGCGGACGACCTGATCGAGGATCTTGACCAGGCACTGCGGAAAGCC
GGGCTCTAA

Upstream 100 bases:

>100_bases
TGGAAGGTGGGCGTCATGCCCGCGCAGGGATAGGGCGCGCGCCGGCGCTCCGCAATCCCGCCGCTTGCACGCAAACCCGC
GCCTGCCTAAGGCAAGCGCC

Downstream 100 bases:

>100_bases
CCTCAGGACGCAATGCGTTTGCATCGTGGCAGACTTGGCCTAAAGTGAAAAACGAGGGTGGGGTCGCAACGGAGCAATCA
GCATTGCTGGCCGAAGCGCA

Product: cystathionine gamma-synthase

Products: methanethiol; NH3; 2-oxobutanoate

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKRTTGQDRSITRKWRPATQAIRGGTWRSEMGETSEALFLTSGFTYDDAATVAARFAGEAEGMTYSRLQNPTVQMLEERI
ALLEGAEACRTQATGMAAMTTALLCQLSAGDHIVAAKAAFGSCRWLVDNLLPRFGVQGTTIDASDNAAWEAAIRPNTKVF
FFESPANPTMDIVDLEFVCGLAKKHGITTVVDNAFATAALQRPMDFGADVVAYSATKMMDGQGRVMAGAVCGSADWINNV
LLPFQRNTGPNIAAFNAWVVLKGLETLDLRIHRQSENALKVASFVEKRVPRLLYPGLPSHPQHALAQKQMKAGGTIFSFD
LDGGRAQAHALLDALQLIDISNNIGDSRSLMCHPASTTHHGVGPETRADMGVGEGMLRLNVGLEDADDLIEDLDQALRKA
GL

Sequences:

>Translated_402_residues
MKRTTGQDRSITRKWRPATQAIRGGTWRSEMGETSEALFLTSGFTYDDAATVAARFAGEAEGMTYSRLQNPTVQMLEERI
ALLEGAEACRTQATGMAAMTTALLCQLSAGDHIVAAKAAFGSCRWLVDNLLPRFGVQGTTIDASDNAAWEAAIRPNTKVF
FFESPANPTMDIVDLEFVCGLAKKHGITTVVDNAFATAALQRPMDFGADVVAYSATKMMDGQGRVMAGAVCGSADWINNV
LLPFQRNTGPNIAAFNAWVVLKGLETLDLRIHRQSENALKVASFVEKRVPRLLYPGLPSHPQHALAQKQMKAGGTIFSFD
LDGGRAQAHALLDALQLIDISNNIGDSRSLMCHPASTTHHGVGPETRADMGVGEGMLRLNVGLEDADDLIEDLDQALRKA
GL
>Mature_402_residues
MKRTTGQDRSITRKWRPATQAIRGGTWRSEMGETSEALFLTSGFTYDDAATVAARFAGEAEGMTYSRLQNPTVQMLEERI
ALLEGAEACRTQATGMAAMTTALLCQLSAGDHIVAAKAAFGSCRWLVDNLLPRFGVQGTTIDASDNAAWEAAIRPNTKVF
FFESPANPTMDIVDLEFVCGLAKKHGITTVVDNAFATAALQRPMDFGADVVAYSATKMMDGQGRVMAGAVCGSADWINNV
LLPFQRNTGPNIAAFNAWVVLKGLETLDLRIHRQSENALKVASFVEKRVPRLLYPGLPSHPQHALAQKQMKAGGTIFSFD
LDGGRAQAHALLDALQLIDISNNIGDSRSLMCHPASTTHHGVGPETRADMGVGEGMLRLNVGLEDADDLIEDLDQALRKA
GL

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=346, Percent_Identity=37.2832369942197, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI299473759, Length=346, Percent_Identity=34.6820809248555, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI25453487, Length=345, Percent_Identity=32.463768115942, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1790375, Length=395, Percent_Identity=34.4303797468354, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1789383, Length=386, Percent_Identity=27.4611398963731, Blast_Score=138, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17538055, Length=376, Percent_Identity=38.031914893617, Blast_Score=238, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI17560056, Length=348, Percent_Identity=38.5057471264368, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI71980708, Length=345, Percent_Identity=36.5217391304348, Blast_Score=213, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6319307, Length=392, Percent_Identity=32.1428571428571, Blast_Score=200, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6323333, Length=408, Percent_Identity=29.9019607843137, Blast_Score=185, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6321170, Length=295, Percent_Identity=29.1525423728814, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6321254, Length=413, Percent_Identity=24.9394673123487, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321904, Length=394, Percent_Identity=25.3807106598985, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19922554, Length=347, Percent_Identity=38.328530259366, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 4.4.1.11

Molecular weight: Translated: 43324; Mature: 43324

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTTGQDRSITRKWRPATQAIRGGTWRSEMGETSEALFLTSGFTYDDAATVAARFAGEA
CCCCCCCCCCHHHHCCHHHHHHCCCCCHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCC
EGMTYSRLQNPTVQMLEERIALLEGAEACRTQATGMAAMTTALLCQLSAGDHIVAAKAAF
CCCCHHHHCCCHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEHHHH
GSCRWLVDNLLPRFGVQGTTIDASDNAAWEAAIRPNTKVFFFESPANPTMDIVDLEFVCG
HHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEECCCCEEEEEECCCCCCEEEEEHHHHHH
LAKKHGITTVVDNAFATAALQRPMDFGADVVAYSATKMMDGQGRVMAGAVCGSADWINNV
HHHHCCCHHHHHHHHHHHHHHCCHHCCCCHHEEHHHHEECCCCCEEEEEECCCHHHHHCC
LLPFQRNTGPNIAAFNAWVVLKGLETLDLRIHRQSENALKVASFVEKRVPRLLYPGLPSH
EEEEECCCCCCEEEEEEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHCCHHCCCCCCCC
PQHALAQKQMKAGGTIFSFDLDGGRAQAHALLDALQLIDISNNIGDSRSLMCHPASTTHH
HHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCC
GVGPETRADMGVGEGMLRLNVGLEDADDLIEDLDQALRKAGL
CCCCCCHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRTTGQDRSITRKWRPATQAIRGGTWRSEMGETSEALFLTSGFTYDDAATVAARFAGEA
CCCCCCCCCCHHHHCCHHHHHHCCCCCHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCC
EGMTYSRLQNPTVQMLEERIALLEGAEACRTQATGMAAMTTALLCQLSAGDHIVAAKAAF
CCCCHHHHCCCHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEHHHH
GSCRWLVDNLLPRFGVQGTTIDASDNAAWEAAIRPNTKVFFFESPANPTMDIVDLEFVCG
HHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEECCCCEEEEEECCCCCCEEEEEHHHHHH
LAKKHGITTVVDNAFATAALQRPMDFGADVVAYSATKMMDGQGRVMAGAVCGSADWINNV
HHHHCCCHHHHHHHHHHHHHHCCHHCCCCHHEEHHHHEECCCCCEEEEEECCCHHHHHCC
LLPFQRNTGPNIAAFNAWVVLKGLETLDLRIHRQSENALKVASFVEKRVPRLLYPGLPSH
EEEEECCCCCCEEEEEEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHCCHHCCCCCCCC
PQHALAQKQMKAGGTIFSFDLDGGRAQAHALLDALQLIDISNNIGDSRSLMCHPASTTHH
HHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCC
GVGPETRADMGVGEGMLRLNVGLEDADDLIEDLDQALRKAGL
CCCCCCHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-methionine

Specific reaction: L-methionine = methanethiol + NH3 + 2-oxobutanoate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]