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The map label for this gene is leuB [H]

Identifier: 87200675

GI number: 87200675

Start: 2899661

End: 2900770

Strand: Direct

Name: leuB [H]

Synonym: Saro_2662

Alternate gene names: 87200675

Gene position: 2899661-2900770 (Clockwise)

Preceding gene: 87200674

Following gene: 87200676

Centisome position: 81.41

GC content: 66.49

Gene sequence:

>1110_bases
TTGACGTCGCGCCGGAAGAAGACGCCGGAAAAGACGGACGAAGGGGTTTCCATGAAGATTGCGGTTCTGCCGGGCGACGG
CATCGGTCCCGAAGTGACGCGCGAAGCGGTGCGCGTGATCGAGGCACTTGGCCTTGGCGACATCGAGATGAAGGAAGCCC
CGGTCGGCGGCGCGGCCTACAAGGCCTATGGCCACCCGCTGCCGCCCGAGACACTGGAGATCGCGCGTCTTTCCGACGGC
ATCCTGTTCGGCGCGGTGGGCGACCCCGACTGCGACTCGCTTGAACGCCACCTGCGCCCGGAACAGGCGATCCTCGGCCT
GCGCAAGGCGCTGACGCTGTTCGCCAACCTGCGCCCCGCGCGGGTGTTCAAGGGCATGGAAGACTTTTCCGCCCTGCGCC
CCGAAGTGGCCGGCGCGATCGACCTGCTGATCGTGCGCGAACTGAACGGTGACGTGTACTTCGGAGAAAAGGGCTTCCGC
ACCGCCGCCAACGGCGACCGCGAAGGCTACGACGTCATGTCCTACAGCGAGAGCGAAGTGCGCCGCATTGCCCACGTCGC
GTTCCGCGCCGCGATGGGCCGCAAGAAGCGCCTGTGCTCGGTCGACAAGGCCAACGTCCTCGAGACCAGCCAGCTCTGGC
GCGACGTGATGCTCGAAGTGGCGAAAGACTATCCCGAAGTCGCGCTCGAGCACATGTACGTCGACAACGCCGCGATGCAG
CTCGTCCGCGCTCCCGGCAACTTCGACGTCGTCGTTACCGGCAACCTGTTCGGCGACATCCTTTCGGACCAGGCATCGAT
GTGCGTCGGCTCGATCGGGCTCCTGGCCTCGGCCTCGCTCGGCGAACGCGAGACCGAATACGGCACGTTCGGCCTGTACG
AGCCGATCCACGGCTCGGCTCCCGACATCGCGGGCAAGGGCCTTGCGAACCCGATGGCCACGATCCTTTCGGCGGCCATG
CTGCTGCGCCACTCGCTCGGCCTCGAAGTGGCTGCGGACCGCATCGAGGCGGCTGTTGCCAAGGCGCTGGCCGACGGCGT
GCTTGGCCGCGACCTTGGCGGAACCGCAGGTACGACGGAAATTGGTGACGCGGTGCTTGCAAGGCTGTAA

Upstream 100 bases:

>100_bases
AGGGCAAGGCAATCGGAAACCGCCTGCTTGCCGATCGAGCCGGCGATGTTATGGCGGCCCGCGCGATTCTGCTGGATCGG
CTGAAGCGCATCGGCGGCGA

Downstream 100 bases:

>100_bases
GGGCCCCGCGTCATGCTGCAACTCGCCGTAATCCTGCCGACTTACAACGAGCGCAAGAACATTGCGCTCATGGTCCAACG
GCTCGACGCCGCCCTTCAGG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MTSRRKKTPEKTDEGVSMKIAVLPGDGIGPEVTREAVRVIEALGLGDIEMKEAPVGGAAYKAYGHPLPPETLEIARLSDG
ILFGAVGDPDCDSLERHLRPEQAILGLRKALTLFANLRPARVFKGMEDFSALRPEVAGAIDLLIVRELNGDVYFGEKGFR
TAANGDREGYDVMSYSESEVRRIAHVAFRAAMGRKKRLCSVDKANVLETSQLWRDVMLEVAKDYPEVALEHMYVDNAAMQ
LVRAPGNFDVVVTGNLFGDILSDQASMCVGSIGLLASASLGERETEYGTFGLYEPIHGSAPDIAGKGLANPMATILSAAM
LLRHSLGLEVAADRIEAAVAKALADGVLGRDLGGTAGTTEIGDAVLARL

Sequences:

>Translated_369_residues
MTSRRKKTPEKTDEGVSMKIAVLPGDGIGPEVTREAVRVIEALGLGDIEMKEAPVGGAAYKAYGHPLPPETLEIARLSDG
ILFGAVGDPDCDSLERHLRPEQAILGLRKALTLFANLRPARVFKGMEDFSALRPEVAGAIDLLIVRELNGDVYFGEKGFR
TAANGDREGYDVMSYSESEVRRIAHVAFRAAMGRKKRLCSVDKANVLETSQLWRDVMLEVAKDYPEVALEHMYVDNAAMQ
LVRAPGNFDVVVTGNLFGDILSDQASMCVGSIGLLASASLGERETEYGTFGLYEPIHGSAPDIAGKGLANPMATILSAAM
LLRHSLGLEVAADRIEAAVAKALADGVLGRDLGGTAGTTEIGDAVLARL
>Mature_368_residues
TSRRKKTPEKTDEGVSMKIAVLPGDGIGPEVTREAVRVIEALGLGDIEMKEAPVGGAAYKAYGHPLPPETLEIARLSDGI
LFGAVGDPDCDSLERHLRPEQAILGLRKALTLFANLRPARVFKGMEDFSALRPEVAGAIDLLIVRELNGDVYFGEKGFRT
AANGDREGYDVMSYSESEVRRIAHVAFRAAMGRKKRLCSVDKANVLETSQLWRDVMLEVAKDYPEVALEHMYVDNAAMQL
VRAPGNFDVVVTGNLFGDILSDQASMCVGSIGLLASASLGERETEYGTFGLYEPIHGSAPDIAGKGLANPMATILSAAML
LRHSLGLEVAADRIEAAVAKALADGVLGRDLGGTAGTTEIGDAVLARL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=368, Percent_Identity=30.4347826086957, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI28178821, Length=359, Percent_Identity=29.8050139275766, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI28178816, Length=358, Percent_Identity=29.3296089385475, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4758582, Length=362, Percent_Identity=29.5580110497238, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI28178838, Length=344, Percent_Identity=29.6511627906977, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI28178819, Length=213, Percent_Identity=33.8028169014084, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI87081683, Length=360, Percent_Identity=47.5, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI1788101, Length=363, Percent_Identity=36.6391184573003, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1787381, Length=405, Percent_Identity=25.9259259259259, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71986051, Length=356, Percent_Identity=31.4606741573034, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17550882, Length=363, Percent_Identity=31.129476584022, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI25144293, Length=361, Percent_Identity=31.8559556786704, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=354, Percent_Identity=28.2485875706215, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=358, Percent_Identity=44.4134078212291, Blast_Score=273, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6322097, Length=370, Percent_Identity=38.6486486486486, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6324709, Length=372, Percent_Identity=31.4516129032258, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324291, Length=366, Percent_Identity=30.6010928961749, Blast_Score=135, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24643268, Length=359, Percent_Identity=33.4261838440111, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24643270, Length=359, Percent_Identity=33.4261838440111, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24661184, Length=361, Percent_Identity=30.7479224376731, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI161078633, Length=363, Percent_Identity=28.099173553719, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24650122, Length=363, Percent_Identity=28.099173553719, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078635, Length=363, Percent_Identity=28.099173553719, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078637, Length=363, Percent_Identity=28.099173553719, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078639, Length=271, Percent_Identity=29.8892988929889, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI281362242, Length=358, Percent_Identity=28.4916201117318, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24648872, Length=358, Percent_Identity=28.4916201117318, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI20130355, Length=359, Percent_Identity=25.0696378830084, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39409; Mature: 39277

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRRKKTPEKTDEGVSMKIAVLPGDGIGPEVTREAVRVIEALGLGDIEMKEAPVGGAAY
CCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
KAYGHPLPPETLEIARLSDGILFGAVGDPDCDSLERHLRPEQAILGLRKALTLFANLRPA
HHCCCCCCCCHHHHHHHCCCEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCHH
RVFKGMEDFSALRPEVAGAIDLLIVRELNGDVYFGEKGFRTAANGDREGYDVMSYSESEV
HHHCCHHHHHHHCHHHHHHHHEEEEEECCCCEEECCCCCCCCCCCCCCCCHHHCCCHHHH
RRIAHVAFRAAMGRKKRLCSVDKANVLETSQLWRDVMLEVAKDYPEVALEHMYVDNAAMQ
HHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LVRAPGNFDVVVTGNLFGDILSDQASMCVGSIGLLASASLGERETEYGTFGLYEPIHGSA
HHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
PDIAGKGLANPMATILSAAMLLRHSLGLEVAADRIEAAVAKALADGVLGRDLGGTAGTTE
CCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
IGDAVLARL
HHHHHHHCC
>Mature Secondary Structure 
TSRRKKTPEKTDEGVSMKIAVLPGDGIGPEVTREAVRVIEALGLGDIEMKEAPVGGAAY
CCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
KAYGHPLPPETLEIARLSDGILFGAVGDPDCDSLERHLRPEQAILGLRKALTLFANLRPA
HHCCCCCCCCHHHHHHHCCCEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCHH
RVFKGMEDFSALRPEVAGAIDLLIVRELNGDVYFGEKGFRTAANGDREGYDVMSYSESEV
HHHCCHHHHHHHCHHHHHHHHEEEEEECCCCEEECCCCCCCCCCCCCCCCHHHCCCHHHH
RRIAHVAFRAAMGRKKRLCSVDKANVLETSQLWRDVMLEVAKDYPEVALEHMYVDNAAMQ
HHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LVRAPGNFDVVVTGNLFGDILSDQASMCVGSIGLLASASLGERETEYGTFGLYEPIHGSA
HHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
PDIAGKGLANPMATILSAAMLLRHSLGLEVAADRIEAAVAKALADGVLGRDLGGTAGTTE
CCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
IGDAVLARL
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA