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The map label for this gene is bcr [H]

Identifier: 87200670

GI number: 87200670

Start: 2894432

End: 2895709

Strand: Direct

Name: bcr [H]

Synonym: Saro_2657

Alternate gene names: 87200670

Gene position: 2894432-2895709 (Clockwise)

Preceding gene: 87200668

Following gene: 87200674

Centisome position: 81.27

GC content: 65.73

Gene sequence:

>1278_bases
ATGGCAGTTATGAACGCTAACAATTCAAACACCTCGACAAGCGCGCGCTTCATGGGGGAGCGCGAAACCATCGCGATGAT
GGCGCTTGTCATGGCGCTCCAGGCGCTCGCGGTCGATTCGATGCTGCCGGCGCTGGGCGACATTGCCCGCGAACTTGCCG
TGACCGATCCGAACGAGCGCCAGCTTGTCGTCGGCACGTTCCTGCTTGCCTCGGGCTTCGCCTCGCTGGTGCCGGGTTCG
CTGGCCGACCGCTACGGGCGGCGGCCGGTGCTGCTGAGCTGCATCGGGCTCTATATCCTGTTCTCGCTGGCCTGCGCGCT
GGCGACGACATTCGACATGCTGCTGGGCATGCGCGTGCTTCAAGCGGTGGGCTGCGGCGGGCTCGCCGTGCTGCCCGGCA
CGATCATCCGCGACCGCTTTGCCGGCGACCAGATGGCCAAGCAGATGTCGATCATCTCGGTCGTGTTCCTCGTCGTGCCG
ATGCTGGCACCAAGCATCGGGCAGGTCGTCCTGCTGTTTGCCGGGTGGCGGTGGATCTTCGTGTTCCTGGCGGTGATGGC
GCTGGCGATGGGCGTGTGGGTGGCCGTGCGCCTGCCAGAGACGCTGCATCCCGAATATCGCCAGCCGATCCGCCCTGCCT
CGATCGCGATCAACATGCTGGGCGCGGCGACGAACCGGTCGGCCATCGGCTATGTTCTGGGCGGCGCGCTGACGTTCGGC
GCGATGATCGGCTACGTGAACTGCTCGCAGCAGCTCGTGGGCGAGCATTTTGGCGCGGGCAAGATGTTCCCGGTGCTGTT
CGGCTTCTCCGCGCTGATGATGGCGGTGGCGAACTTCTCGAACTCGCGCATCGTCGAGCGGTTCGGCGCGCGGCGGGTTT
CGCACACCGCCGTGATCCTGTTCATCTGCGTGAGCGCGCTGCAGGCCTGGTTCTCCAGCCGCCCGGACGAAACACTGTGG
CAGTTCATGCCGCTGATGATCGCCAACATGATCCTCATCGGGTTCATCGGCGCGAACTTCGGCTCGATCGCGTTGCAGCC
CTTTGCGCGCACCGCAGGCACGGCCAATTCGATGCATGCGTTCCTGCGCATGGTCATCGGGTCGATCATCGGCATCGTGG
TGGGCCAGGCCTATGACGGAACCGCCCACCCCCTGGGACTTGCCCTGCTGGCGAGCGGGATACTGAGCCTCGCGCTGGTG
CTGTTCAGCGAGAAGGGCAAGCTGTTCCGGCGCCTGCACCCGCCGGGAACGCCGCGCCCCATCGTGCTGGAGCACTGA

Upstream 100 bases:

>100_bases
CGATCCGGTTCGCCATCTGCCGATCGAGGGTCCATTTCGCGATGCTAACCCGTTTCGCATTGCAACATAAAGGGTGGGAT
TCGCGTGCGAACTAGTTCAA

Downstream 100 bases:

>100_bases
TCAGGCGACGCGGGCGAGGTTCGACAGTACCTGCTCGGCCTCGTCCGGCCGCCCGAAGTAATAGCCCTGCATCAGGCGGA
TGCCGAGCGCGCGGGCCGTG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MAVMNANNSNTSTSARFMGERETIAMMALVMALQALAVDSMLPALGDIARELAVTDPNERQLVVGTFLLASGFASLVPGS
LADRYGRRPVLLSCIGLYILFSLACALATTFDMLLGMRVLQAVGCGGLAVLPGTIIRDRFAGDQMAKQMSIISVVFLVVP
MLAPSIGQVVLLFAGWRWIFVFLAVMALAMGVWVAVRLPETLHPEYRQPIRPASIAINMLGAATNRSAIGYVLGGALTFG
AMIGYVNCSQQLVGEHFGAGKMFPVLFGFSALMMAVANFSNSRIVERFGARRVSHTAVILFICVSALQAWFSSRPDETLW
QFMPLMIANMILIGFIGANFGSIALQPFARTAGTANSMHAFLRMVIGSIIGIVVGQAYDGTAHPLGLALLASGILSLALV
LFSEKGKLFRRLHPPGTPRPIVLEH

Sequences:

>Translated_425_residues
MAVMNANNSNTSTSARFMGERETIAMMALVMALQALAVDSMLPALGDIARELAVTDPNERQLVVGTFLLASGFASLVPGS
LADRYGRRPVLLSCIGLYILFSLACALATTFDMLLGMRVLQAVGCGGLAVLPGTIIRDRFAGDQMAKQMSIISVVFLVVP
MLAPSIGQVVLLFAGWRWIFVFLAVMALAMGVWVAVRLPETLHPEYRQPIRPASIAINMLGAATNRSAIGYVLGGALTFG
AMIGYVNCSQQLVGEHFGAGKMFPVLFGFSALMMAVANFSNSRIVERFGARRVSHTAVILFICVSALQAWFSSRPDETLW
QFMPLMIANMILIGFIGANFGSIALQPFARTAGTANSMHAFLRMVIGSIIGIVVGQAYDGTAHPLGLALLASGILSLALV
LFSEKGKLFRRLHPPGTPRPIVLEH
>Mature_424_residues
AVMNANNSNTSTSARFMGERETIAMMALVMALQALAVDSMLPALGDIARELAVTDPNERQLVVGTFLLASGFASLVPGSL
ADRYGRRPVLLSCIGLYILFSLACALATTFDMLLGMRVLQAVGCGGLAVLPGTIIRDRFAGDQMAKQMSIISVVFLVVPM
LAPSIGQVVLLFAGWRWIFVFLAVMALAMGVWVAVRLPETLHPEYRQPIRPASIAINMLGAATNRSAIGYVLGGALTFGA
MIGYVNCSQQLVGEHFGAGKMFPVLFGFSALMMAVANFSNSRIVERFGARRVSHTAVILFICVSALQAWFSSRPDETLWQ
FMPLMIANMILIGFIGANFGSIALQPFARTAGTANSMHAFLRMVIGSIIGIVVGQAYDGTAHPLGLALLASGILSLALVL
FSEKGKLFRRLHPPGTPRPIVLEH

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=388, Percent_Identity=26.2886597938144, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1790146, Length=392, Percent_Identity=28.0612244897959, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI87082312, Length=160, Percent_Identity=32.5, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1790195, Length=172, Percent_Identity=30.2325581395349, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI48994889, Length=354, Percent_Identity=25.1412429378531, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790794, Length=171, Percent_Identity=26.9005847953216, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45526; Mature: 45395

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVMNANNSNTSTSARFMGERETIAMMALVMALQALAVDSMLPALGDIARELAVTDPNER
CCEECCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QLVVGTFLLASGFASLVPGSLADRYGRRPVLLSCIGLYILFSLACALATTFDMLLGMRVL
EEHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAVGCGGLAVLPGTIIRDRFAGDQMAKQMSIISVVFLVVPMLAPSIGQVVLLFAGWRWIF
HHHCCCCHHHCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
VFLAVMALAMGVWVAVRLPETLHPEYRQPIRPASIAINMLGAATNRSAIGYVLGGALTFG
HHHHHHHHHHHHHHHHCCCHHCCHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AMIGYVNCSQQLVGEHFGAGKMFPVLFGFSALMMAVANFSNSRIVERFGARRVSHTAVIL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHH
FICVSALQAWFSSRPDETLWQFMPLMIANMILIGFIGANFGSIALQPFARTAGTANSMHA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHH
FLRMVIGSIIGIVVGQAYDGTAHPLGLALLASGILSLALVLFSEKGKLFRRLHPPGTPRP
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCC
IVLEH
CEECC
>Mature Secondary Structure 
AVMNANNSNTSTSARFMGERETIAMMALVMALQALAVDSMLPALGDIARELAVTDPNER
CEECCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC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CEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]