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The map label for this gene is acsA

Identifier: 87200638

GI number: 87200638

Start: 2840827

End: 2842776

Strand: Direct

Name: acsA

Synonym: Saro_2625

Alternate gene names: 87200638

Gene position: 2840827-2842776 (Clockwise)

Preceding gene: 87200627

Following gene: 87200639

Centisome position: 79.76

GC content: 64.77

Gene sequence:

>1950_bases
ATGGGCGAGGCAGTCTATCCTGTACCGGCTGAGTGGGCGCAGAACGCGCTGGTCGACGAGGCGCGCTACCAGGATATGTA
TCGCCAGTCGGTCGACGATCCCGAAGGCTTCTGGGCCGAACACGGCAAGCGGATCGATTGGATCAGGCCTTTCACCAAGG
TCAAGAACACCTCGTTTCACGAAGCCGACTTCGGCATCCGCTGGTTCGAGGACGGCACGCTCAACCTCGCCGCGAACTGC
CTCGACCGTCACCTCGCCGAACGCGGCGACGAGATCGCGATCCTGTGGGAGCCCGACAGCCCCGACGAGGCACACCGCGA
GATCACCTACCGCCAGCTCCACGCCGATGTCTGCCGCTTTGCCAACCTGCTGAAGGCCAAGGGCGTCCAGAAGGGCGAGC
GCGTCACCATCTACCTGCCGATGGTGCCGGAAGCCGCCGTGGCGATGCTGGCCTGCGCCCGCATCGGCGCGATCCATTCC
ATCGTCTTTGCAGGCTTCAGCCCGGATGCGCTGGCCGGCCGCATCACCGACTGCGACAGCCGCATCGTCCTCACCTCGGA
CGAAGGCCTGCGCGGCGGACGCAAGGTTCCGCTGAAGGCCAATGTGGACGAGGCGCTGAAGCAATGCCCCGGCGTCGACA
CCGTGATCATGCTGCGCCGCACGGGCGCCGACGTGGACTTTGTCGAAGGCCGCGACATCGACTGGGCAACGGCAGTCGCC
GAGCAGAGCGCCGATTGCCAGCCCGAGGAAATGAACGCGGAAGACCCGCTGTTCATCCTCTACACCTCCGGCTCTACCGG
AAAGCCCAAGGGCGTGCTGCACACGACGGGCGGCTATTCGGTCTGGGCGTCGATGACGCACCAGTACGTGTTCGATTACC
GTCCCGGGCAGATCTACTGGTGCGCGGCCGACATCGGCTGGGTCACCGGCCACTCCTATGTCGTCTACGGACCGCTCATG
AACGGCGCCACTACGGTCATGTTTGAAGGCGTGCCGAACTTCCCGGACGCAAGCCGCTTCTGGCAGGTGGTCGACAAGTT
CAAGGTCGAAATCTTCTACGGCGCCCCTACCGCGCTGCGCGCCCTGATGCGCGAGGGCGACGAATGGGTGAAGAAGACGA
GCCGCGCCTCGCTGCGCCTGCTTGGTTCGGTGGGCGAACCGATCAATCCCGAAGCCTGGGAATGGTATCACAAGGTCGTA
GGCGACAGTCGCTGCCCGATCGTCGACACCTGGTGGCAGACCGAAACCGGCGGGGCGATGATCACCCCGCTACCGGGCGC
GACCGCGCTCAAGCCCGGCTCGGCCTCGCGCCCGTTCTTCGGCGTAAAGCCCGCGCTTGTCGATAACGACGGCACGTTCC
TTGAAGGCGCGACTGACGGGTGCCTCGTAGTGACCGATAGCTGGCCCGGCCAGATGCGCACCGTGTGGGGCGATCACGAG
CGGTTCTTCCAGACCTATTTCACCACTTTCAAGGGCCTGTACTTCACCGGCGACGGATGCCGCCGCGACGAGGACGGCTA
CTACTGGATCACCGGCCGCATCGACGACGTGATCAACGTGTCGGGCCACCGCATGGGAACGGCGGAAATCGAAAGCGCGC
TGGTCGCCCACCCGAAGGTGGCCGAAGCTGCGGTCGTCGGCATGCCGCACGACATCAAGGGCCAGGGCATCTACGCCTTC
GTCACCTGCAATGCCGAAATCGAGCCCGACGACCTGCTGCGCAAGGAGTTGATCCAGTGGGTCCGCCACGAAATCGGCCC
CATCGCCACGCCGGACGTGATCCAGTTCGCTCCCGGTCTGCCCAAAACCCGTTCGGGCAAGATCATGCGCCGCATCCTGC
GCAAGATCGGCGAGAACGACGTCTCCAACCTTGGCGACACGTCGACCCTTGCCGATCCGTCGGTTGTCGATAATCTGCTG
GCCAACAGACCGCAGCTCGCAGGGGCTTGA

Upstream 100 bases:

>100_bases
GAGGATGGTCTTATATCTACGACTATTGTCTAGAAGCCCCGCATGGCCGGGGTGCGGTAACACGCCCGGCAAGAGCAGAA
TCTGAAAAGGAGAGCCTGAA

Downstream 100 bases:

>100_bases
ACGCGGACTGTCCGGAGGGGCGTGAGCGGAACAATCCTGATTGCCGACGACCATCCGCTGTTCCGCCAGGCCCTCGCCCT
GGCGGTTTCAGCGGTGGCCC

Product: acetyl-coenzyme A synthetase

Products: NA

Alternate protein names: Acetate--CoA ligase; Acyl-activating enzyme

Number of amino acids: Translated: 649; Mature: 648

Protein sequence:

>649_residues
MGEAVYPVPAEWAQNALVDEARYQDMYRQSVDDPEGFWAEHGKRIDWIRPFTKVKNTSFHEADFGIRWFEDGTLNLAANC
LDRHLAERGDEIAILWEPDSPDEAHREITYRQLHADVCRFANLLKAKGVQKGERVTIYLPMVPEAAVAMLACARIGAIHS
IVFAGFSPDALAGRITDCDSRIVLTSDEGLRGGRKVPLKANVDEALKQCPGVDTVIMLRRTGADVDFVEGRDIDWATAVA
EQSADCQPEEMNAEDPLFILYTSGSTGKPKGVLHTTGGYSVWASMTHQYVFDYRPGQIYWCAADIGWVTGHSYVVYGPLM
NGATTVMFEGVPNFPDASRFWQVVDKFKVEIFYGAPTALRALMREGDEWVKKTSRASLRLLGSVGEPINPEAWEWYHKVV
GDSRCPIVDTWWQTETGGAMITPLPGATALKPGSASRPFFGVKPALVDNDGTFLEGATDGCLVVTDSWPGQMRTVWGDHE
RFFQTYFTTFKGLYFTGDGCRRDEDGYYWITGRIDDVINVSGHRMGTAEIESALVAHPKVAEAAVVGMPHDIKGQGIYAF
VTCNAEIEPDDLLRKELIQWVRHEIGPIATPDVIQFAPGLPKTRSGKIMRRILRKIGENDVSNLGDTSTLADPSVVDNLL
ANRPQLAGA

Sequences:

>Translated_649_residues
MGEAVYPVPAEWAQNALVDEARYQDMYRQSVDDPEGFWAEHGKRIDWIRPFTKVKNTSFHEADFGIRWFEDGTLNLAANC
LDRHLAERGDEIAILWEPDSPDEAHREITYRQLHADVCRFANLLKAKGVQKGERVTIYLPMVPEAAVAMLACARIGAIHS
IVFAGFSPDALAGRITDCDSRIVLTSDEGLRGGRKVPLKANVDEALKQCPGVDTVIMLRRTGADVDFVEGRDIDWATAVA
EQSADCQPEEMNAEDPLFILYTSGSTGKPKGVLHTTGGYSVWASMTHQYVFDYRPGQIYWCAADIGWVTGHSYVVYGPLM
NGATTVMFEGVPNFPDASRFWQVVDKFKVEIFYGAPTALRALMREGDEWVKKTSRASLRLLGSVGEPINPEAWEWYHKVV
GDSRCPIVDTWWQTETGGAMITPLPGATALKPGSASRPFFGVKPALVDNDGTFLEGATDGCLVVTDSWPGQMRTVWGDHE
RFFQTYFTTFKGLYFTGDGCRRDEDGYYWITGRIDDVINVSGHRMGTAEIESALVAHPKVAEAAVVGMPHDIKGQGIYAF
VTCNAEIEPDDLLRKELIQWVRHEIGPIATPDVIQFAPGLPKTRSGKIMRRILRKIGENDVSNLGDTSTLADPSVVDNLL
ANRPQLAGA
>Mature_648_residues
GEAVYPVPAEWAQNALVDEARYQDMYRQSVDDPEGFWAEHGKRIDWIRPFTKVKNTSFHEADFGIRWFEDGTLNLAANCL
DRHLAERGDEIAILWEPDSPDEAHREITYRQLHADVCRFANLLKAKGVQKGERVTIYLPMVPEAAVAMLACARIGAIHSI
VFAGFSPDALAGRITDCDSRIVLTSDEGLRGGRKVPLKANVDEALKQCPGVDTVIMLRRTGADVDFVEGRDIDWATAVAE
QSADCQPEEMNAEDPLFILYTSGSTGKPKGVLHTTGGYSVWASMTHQYVFDYRPGQIYWCAADIGWVTGHSYVVYGPLMN
GATTVMFEGVPNFPDASRFWQVVDKFKVEIFYGAPTALRALMREGDEWVKKTSRASLRLLGSVGEPINPEAWEWYHKVVG
DSRCPIVDTWWQTETGGAMITPLPGATALKPGSASRPFFGVKPALVDNDGTFLEGATDGCLVVTDSWPGQMRTVWGDHER
FFQTYFTTFKGLYFTGDGCRRDEDGYYWITGRIDDVINVSGHRMGTAEIESALVAHPKVAEAAVVGMPHDIKGQGIYAFV
TCNAEIEPDDLLRKELIQWVRHEIGPIATPDVIQFAPGLPKTRSGKIMRRILRKIGENDVSNLGDTSTLADPSVVDNLLA
NRPQLAGA

Specific function: Enables The Cell To Use Acetate During Aerobic Growth To Generate Energy Via The Tca Cycle, And Biosynthetic Compounds Via The Glyoxylate Shunt. Acetylates Chey, The Response Regulator Involved In Flagellar Movement And Chemotaxis. [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family

Homologues:

Organism=Homo sapiens, GI8923896, Length=668, Percent_Identity=53.5928143712575, Blast_Score=702, Evalue=0.0,
Organism=Homo sapiens, GI253970489, Length=681, Percent_Identity=52.5697503671072, Blast_Score=697, Evalue=0.0,
Organism=Homo sapiens, GI28416953, Length=624, Percent_Identity=51.7628205128205, Blast_Score=668, Evalue=0.0,
Organism=Homo sapiens, GI13375727, Length=634, Percent_Identity=38.01261829653, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI42544132, Length=514, Percent_Identity=29.9610894941634, Blast_Score=200, Evalue=4e-51,
Organism=Homo sapiens, GI31982927, Length=644, Percent_Identity=25.776397515528, Blast_Score=192, Evalue=1e-48,
Organism=Homo sapiens, GI122937307, Length=554, Percent_Identity=27.7978339350181, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI115511026, Length=553, Percent_Identity=28.0289330922242, Blast_Score=190, Evalue=4e-48,
Organism=Homo sapiens, GI38505220, Length=512, Percent_Identity=28.7109375, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI58082049, Length=509, Percent_Identity=27.5049115913556, Blast_Score=176, Evalue=6e-44,
Organism=Homo sapiens, GI157311624, Length=513, Percent_Identity=27.4853801169591, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI157311622, Length=513, Percent_Identity=27.4853801169591, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI187761345, Length=576, Percent_Identity=26.3888888888889, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI187761343, Length=576, Percent_Identity=26.3888888888889, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI195947378, Length=408, Percent_Identity=25.7352941176471, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI42544134, Length=333, Percent_Identity=26.1261261261261, Blast_Score=104, Evalue=3e-22,
Organism=Escherichia coli, GI1790505, Length=644, Percent_Identity=66.9254658385093, Blast_Score=914, Evalue=0.0,
Organism=Escherichia coli, GI1786529, Length=629, Percent_Identity=38.4737678855326, Blast_Score=420, Evalue=1e-118,
Organism=Escherichia coli, GI221142682, Length=521, Percent_Identity=23.4165067178503, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI145693145, Length=543, Percent_Identity=23.9410681399632, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1788107, Length=544, Percent_Identity=24.6323529411765, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1786810, Length=566, Percent_Identity=21.5547703180212, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71983001, Length=642, Percent_Identity=49.5327102803738, Blast_Score=619, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI71982997, Length=642, Percent_Identity=49.5327102803738, Blast_Score=617, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI17569507, Length=642, Percent_Identity=21.3395638629283, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=553, Percent_Identity=24.4122965641953, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=527, Percent_Identity=23.719165085389, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17559526, Length=586, Percent_Identity=24.061433447099, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=516, Percent_Identity=23.2558139534884, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17538037, Length=138, Percent_Identity=34.7826086956522, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323182, Length=642, Percent_Identity=47.6635514018692, Blast_Score=617, Evalue=1e-177,
Organism=Saccharomyces cerevisiae, GI6319264, Length=638, Percent_Identity=47.4921630094044, Blast_Score=595, Evalue=1e-171,
Organism=Saccharomyces cerevisiae, GI6319699, Length=367, Percent_Identity=24.2506811989101, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24667955, Length=659, Percent_Identity=54.4764795144158, Blast_Score=712, Evalue=0.0,
Organism=Drosophila melanogaster, GI62472339, Length=563, Percent_Identity=57.9040852575488, Blast_Score=660, Evalue=0.0,
Organism=Drosophila melanogaster, GI24667959, Length=521, Percent_Identity=58.1573896353167, Blast_Score=611, Evalue=1e-175,
Organism=Drosophila melanogaster, GI21355225, Length=634, Percent_Identity=37.0662460567823, Blast_Score=384, Evalue=1e-106,
Organism=Drosophila melanogaster, GI18859661, Length=537, Percent_Identity=26.2569832402235, Blast_Score=110, Evalue=5e-24,
Organism=Drosophila melanogaster, GI21355181, Length=515, Percent_Identity=24.0776699029126, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24581924, Length=380, Percent_Identity=22.3684210526316, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648260, Length=372, Percent_Identity=25, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACSA_NOVAD (Q2G512)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497895.1
- ProteinModelPortal:   Q2G512
- SMR:   Q2G512
- STRING:   Q2G512
- GeneID:   3917040
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2625
- NMPDR:   fig|48935.1.peg.2373
- eggNOG:   COG0365
- HOGENOM:   HBG547964
- OMA:   LVHEANE
- PhylomeDB:   Q2G512
- BioCyc:   NARO279238:SARO_2625-MONOMER
- HAMAP:   MF_01123
- InterPro:   IPR011904
- InterPro:   IPR020845
- InterPro:   IPR000873
- TIGRFAMs:   TIGR02188

Pfam domain/function: PF00501 AMP-binding

EC number: =6.2.1.1

Molecular weight: Translated: 71888; Mature: 71757

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00455 AMP_BINDING

Important sites: ACT_SITE 515-515

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEAVYPVPAEWAQNALVDEARYQDMYRQSVDDPEGFWAEHGKRIDWIRPFTKVKNTSFH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEECHHCCHHHHCCCCCC
EADFGIRWFEDGTLNLAANCLDRHLAERGDEIAILWEPDSPDEAHREITYRQLHADVCRF
CCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
ANLLKAKGVQKGERVTIYLPMVPEAAVAMLACARIGAIHSIVFAGFSPDALAGRITDCDS
HHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEECCCC
RIVLTSDEGLRGGRKVPLKANVDEALKQCPGVDTVIMLRRTGADVDFVEGRDIDWATAVA
EEEEECCCCCCCCCCCCEECCHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCCHHHHHH
EQSADCQPEEMNAEDPLFILYTSGSTGKPKGVLHTTGGYSVWASMTHQYVFDYRPGQIYW
HCCCCCCHHHCCCCCCEEEEEECCCCCCCCCEEECCCCHHHHHHHHHEEEEEECCCEEEE
CAADIGWVTGHSYVVYGPLMNGATTVMFEGVPNFPDASRFWQVVDKFKVEIFYGAPTALR
EECCCCEECCCCEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHEEEEEEECCHHHHH
ALMREGDEWVKKTSRASLRLLGSVGEPINPEAWEWYHKVVGDSRCPIVDTWWQTETGGAM
HHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCEE
ITPLPGATALKPGSASRPFFGVKPALVDNDGTFLEGATDGCLVVTDSWPGQMRTVWGDHE
EECCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEECCCCCEEEEECCCCCCEEECCCCHH
RFFQTYFTTFKGLYFTGDGCRRDEDGYYWITGRIDDVINVSGHRMGTAEIESALVAHPKV
HHHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHCCCCH
AEAAVVGMPHDIKGQGIYAFVTCNAEIEPDDLLRKELIQWVRHEIGPIATPDVIQFAPGL
HHHEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCC
PKTRSGKIMRRILRKIGENDVSNLGDTSTLADPSVVDNLLANRPQLAGA
CCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GEAVYPVPAEWAQNALVDEARYQDMYRQSVDDPEGFWAEHGKRIDWIRPFTKVKNTSFH
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEECHHCCHHHHCCCCCC
EADFGIRWFEDGTLNLAANCLDRHLAERGDEIAILWEPDSPDEAHREITYRQLHADVCRF
CCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
ANLLKAKGVQKGERVTIYLPMVPEAAVAMLACARIGAIHSIVFAGFSPDALAGRITDCDS
HHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEECCCC
RIVLTSDEGLRGGRKVPLKANVDEALKQCPGVDTVIMLRRTGADVDFVEGRDIDWATAVA
EEEEECCCCCCCCCCCCEECCHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCCHHHHHH
EQSADCQPEEMNAEDPLFILYTSGSTGKPKGVLHTTGGYSVWASMTHQYVFDYRPGQIYW
HCCCCCCHHHCCCCCCEEEEEECCCCCCCCCEEECCCCHHHHHHHHHEEEEEECCCEEEE
CAADIGWVTGHSYVVYGPLMNGATTVMFEGVPNFPDASRFWQVVDKFKVEIFYGAPTALR
EECCCCEECCCCEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHEEEEEEECCHHHHH
ALMREGDEWVKKTSRASLRLLGSVGEPINPEAWEWYHKVVGDSRCPIVDTWWQTETGGAM
HHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCEE
ITPLPGATALKPGSASRPFFGVKPALVDNDGTFLEGATDGCLVVTDSWPGQMRTVWGDHE
EECCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEECCCCCEEEEECCCCCCEEECCCCHH
RFFQTYFTTFKGLYFTGDGCRRDEDGYYWITGRIDDVINVSGHRMGTAEIESALVAHPKV
HHHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHCCCCH
AEAAVVGMPHDIKGQGIYAFVTCNAEIEPDDLLRKELIQWVRHEIGPIATPDVIQFAPGL
HHHEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCC
PKTRSGKIMRRILRKIGENDVSNLGDTSTLADPSVVDNLLANRPQLAGA
CCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA