Click here to switch to the map view.

The map label for this gene is proP [H]

Identifier: 87200636

GI number: 87200636

Start: 2837522

End: 2839213

Strand: Reverse

Name: proP [H]

Synonym: Saro_2623

Alternate gene names: 87200636

Gene position: 2839213-2837522 (Counterclockwise)

Preceding gene: 87200637

Following gene: 87200635

Centisome position: 79.72

GC content: 63.18

Gene sequence:

>1692_bases
ATGTCCAACCTTGAGATAGCGGATGATTTTTCGGCTTTGCCGAAACATCATCAGGCCACGCACAACGAGAAGCTGATCAT
AACCGCGTCTTCGCTAGGCACGGTGTTCGAGTGGTACGACTTCTACCTCTATGGTCTCCTCACCGCGATCATCGCGGCGA
AGTTCCTGACAGGGCTCAACCCGACGACCTCGTTCATCATGGCGCTGCTCGTCTTCGCGGCGGGCTTCATCGTCCGTCCT
TTCGGGGCACTCGTGTTCGGCCGCATCGGGGACATGGTGGGCCGCCGCTATACCTTCATCGTGACGCTGCTGGTCATGGG
CCTGTCGACCTTCCTTGTCGGTTGCCTGCCCACCTATGAAACCGTCGGCGTGGCCGCGCCGATCATGCTCGTCGTCCTGC
GCATGTTCCAGGGCCTGGCCCTGGGCGGGGAATACGGCGGCGCCGCAACCTACGTGGCCGAGCACGCGCCCGAAGGAAAG
CGGGGGCTCTATACGAGCTGGATCCAGATCACCGCGACGGCCGGGCTGGCCATGGCCTTGCTGATCGTGATCCTCGTGCG
CTCGCCGGTCACCGGCGTGGGTGAGGAGGCGTTCAAGGACTGGGGCTGGCGCGTGCCCTACCTGATCTCGGGCCTGTTCC
TCTGCGTGGGGCTGTGGCTGCGCCTGAAGCTGCATGAATCGCCCGTCTTCCAGAAGATGAAGGACGAAGGCACATCCTCC
AAGCGTCCGCTGGGCGAAGCCTTCGGCGAATGGAAGAACCTCAAGATCGTGCTGATCGCGTTCTTCGGCGCCATCGCGGG
CCAGGCCGTGGTCTGGTACACCGGCCAGCTCTACGCGATGTACTTTCTCGAAAAGATGCTCAAGGTCGATGGCCTTACCG
CCAACACGCTGATCATCGTCGCGCTTGCCTGTGCCACGCCGTTCTTCCTGTTCTTCGGCTGGCTGTCGGACAAGATCGGT
CGCAAGAAGATCATCCTGGCCGGTTGCGCGCTCGCTGCGCTCACCATGTTCCCGGCGTTCAAGGCGCTGACCGAAGCGGC
GAACCCCGCCCTTGCCCATGCCCAGGCCAATGCGCCGGTAAAGGTCTTGGCCAACCCCGCCGAATGCTCGTCGCAGTTCG
ACCCGGTAGGCGCGAACAAGTTCGACACCACAAGCTGCGACATCGTCAAGAACGCGCTCGCCAAGGCGGCGGTGAACTAC
GAGAACGTCGCGGCACCCGCCGGCACCATTGCTTCGATCCGGATCGGCAGCACCACCATCGTCGCTCCCGATCCGTCGAA
GGTTTCGGGTGACGAGAAGAAGGCCGCCATCGCCGCCTTCGCCAAGCAGGTCACCTCCGACCTGGAGACCGTGGGCTACC
CGGCCAAGGCCGACCCTGACCAGATCGACAAGCCGGTCGTCGTGGCGATCCTGTTCTATCTCGTCCTGCTGGTGACCATG
GTCTACGGTCCCATCGCGGCGATGCTGGTCGAACTGTTCCCCAGCCGCATCCGCTACACCTCGATGAGCCTGCCGTACCA
CATCGGCAATGGCTGGCTCGGCGGCCTGCTCCCGGCCATCGGTTTTGCCATGGTCGCGGCCAACGGGGATATCTACCACG
GCTTCTGGTACCCGGTGATCGTGGCCGCCGCCACTTGCGTCATAGGCCTGGTGTTCCTGCCGGAGACGTACAAGCGGAGC
ATCGACGACTAA

Upstream 100 bases:

>100_bases
GGTGAGCGCAAGCTCGTCAACGGTGCGAGCGGTTCGCTCGATCGCATCGAGTTCGCCGCGAAGTACGGTTTCTAAGAGCC
CACAATAATAGGGGAAGAGG

Downstream 100 bases:

>100_bases
GGTCTTACTGGAGCGGGTAGCGGTTTGGTCCGATACCCGCTCCAACCGTTTGAAGAGTCGGGTGAGGACATGAGCGAAGC
ATACGACCGGGCATGGCAGG

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MSNLEIADDFSALPKHHQATHNEKLIITASSLGTVFEWYDFYLYGLLTAIIAAKFLTGLNPTTSFIMALLVFAAGFIVRP
FGALVFGRIGDMVGRRYTFIVTLLVMGLSTFLVGCLPTYETVGVAAPIMLVVLRMFQGLALGGEYGGAATYVAEHAPEGK
RGLYTSWIQITATAGLAMALLIVILVRSPVTGVGEEAFKDWGWRVPYLISGLFLCVGLWLRLKLHESPVFQKMKDEGTSS
KRPLGEAFGEWKNLKIVLIAFFGAIAGQAVVWYTGQLYAMYFLEKMLKVDGLTANTLIIVALACATPFFLFFGWLSDKIG
RKKIILAGCALAALTMFPAFKALTEAANPALAHAQANAPVKVLANPAECSSQFDPVGANKFDTTSCDIVKNALAKAAVNY
ENVAAPAGTIASIRIGSTTIVAPDPSKVSGDEKKAAIAAFAKQVTSDLETVGYPAKADPDQIDKPVVVAILFYLVLLVTM
VYGPIAAMLVELFPSRIRYTSMSLPYHIGNGWLGGLLPAIGFAMVAANGDIYHGFWYPVIVAAATCVIGLVFLPETYKRS
IDD

Sequences:

>Translated_563_residues
MSNLEIADDFSALPKHHQATHNEKLIITASSLGTVFEWYDFYLYGLLTAIIAAKFLTGLNPTTSFIMALLVFAAGFIVRP
FGALVFGRIGDMVGRRYTFIVTLLVMGLSTFLVGCLPTYETVGVAAPIMLVVLRMFQGLALGGEYGGAATYVAEHAPEGK
RGLYTSWIQITATAGLAMALLIVILVRSPVTGVGEEAFKDWGWRVPYLISGLFLCVGLWLRLKLHESPVFQKMKDEGTSS
KRPLGEAFGEWKNLKIVLIAFFGAIAGQAVVWYTGQLYAMYFLEKMLKVDGLTANTLIIVALACATPFFLFFGWLSDKIG
RKKIILAGCALAALTMFPAFKALTEAANPALAHAQANAPVKVLANPAECSSQFDPVGANKFDTTSCDIVKNALAKAAVNY
ENVAAPAGTIASIRIGSTTIVAPDPSKVSGDEKKAAIAAFAKQVTSDLETVGYPAKADPDQIDKPVVVAILFYLVLLVTM
VYGPIAAMLVELFPSRIRYTSMSLPYHIGNGWLGGLLPAIGFAMVAANGDIYHGFWYPVIVAAATCVIGLVFLPETYKRS
IDD
>Mature_562_residues
SNLEIADDFSALPKHHQATHNEKLIITASSLGTVFEWYDFYLYGLLTAIIAAKFLTGLNPTTSFIMALLVFAAGFIVRPF
GALVFGRIGDMVGRRYTFIVTLLVMGLSTFLVGCLPTYETVGVAAPIMLVVLRMFQGLALGGEYGGAATYVAEHAPEGKR
GLYTSWIQITATAGLAMALLIVILVRSPVTGVGEEAFKDWGWRVPYLISGLFLCVGLWLRLKLHESPVFQKMKDEGTSSK
RPLGEAFGEWKNLKIVLIAFFGAIAGQAVVWYTGQLYAMYFLEKMLKVDGLTANTLIIVALACATPFFLFFGWLSDKIGR
KKIILAGCALAALTMFPAFKALTEAANPALAHAQANAPVKVLANPAECSSQFDPVGANKFDTTSCDIVKNALAKAAVNYE
NVAAPAGTIASIRIGSTTIVAPDPSKVSGDEKKAAIAAFAKQVTSDLETVGYPAKADPDQIDKPVVVAILFYLVLLVTMV
YGPIAAMLVELFPSRIRYTSMSLPYHIGNGWLGGLLPAIGFAMVAANGDIYHGFWYPVIVAAATCVIGLVFLPETYKRSI
DD

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=325, Percent_Identity=35.0769230769231, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788942, Length=333, Percent_Identity=34.2342342342342, Blast_Score=179, Evalue=6e-46,
Organism=Escherichia coli, GI1788292, Length=318, Percent_Identity=34.2767295597484, Blast_Score=179, Evalue=6e-46,
Organism=Escherichia coli, GI1789941, Length=345, Percent_Identity=35.0724637681159, Blast_Score=165, Evalue=7e-42,
Organism=Escherichia coli, GI87082231, Length=290, Percent_Identity=26.551724137931, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87082404, Length=325, Percent_Identity=25.2307692307692, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 60594; Mature: 60463

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCHHHHHCCCC
>Mature Secondary Structure 
SNLEIADDFSALPKHHQATHNEKLIITASSLGTVFEWYDFYLYGLLTAIIAAKFLTGLN
CCCCCCCHHHHCCCHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC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HHHHHHHHHHHHCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]