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The map label for this gene is thcR [H]

Identifier: 87200624

GI number: 87200624

Start: 2823727

End: 2824686

Strand: Reverse

Name: thcR [H]

Synonym: Saro_2611

Alternate gene names: 87200624

Gene position: 2824686-2823727 (Counterclockwise)

Preceding gene: 87200628

Following gene: 87200620

Centisome position: 79.31

GC content: 66.04

Gene sequence:

>960_bases
ATGTATCCAGAATGCGCAAAGCTTCCGACTTTTCTCAGGACAGTGCATGAAGATGTCGATGCGCAGGCCAGAAGCCTCTT
CACGCTCGACCAGAACTACATGCAGGTCGATCCGGGGCCGTTCTGCGGGACCATGGCCACCTGCGATTTCGGCACCGGCC
TCGTCGTCTCGTTCGAACAGGTGAACAAGCGCGTGCTGCAGGTCGCGGCTGTTCCTTTCGGCACCGCACAGGTCGCGCTG
ATACTCGAGGCGGGCGAGCCGACGACCCACAACCAGGTCGGGCGGATCCGGAACGCGGGGGTAGTCATGGCCCCCGGTGC
CGAAGCGCGCCTGAGCGCGGGCGCCAACACCCGCGTCTGCCTGATTTCCATCGCCGGAGAGGCGGCCGATCACCTGCGGT
CACGTTACAACTTCGATCCCGACAGCTTGCGCGCAGACATCTTCCTCGATCCGGCGGAAACCACCGCGCTGCGCGGGCTC
GTGACCGAGGCCTACCAGCGCTTCAGCAGTCCCCAGGCCGACAGGTCATTCGACCAGGCAAGCGCCGCCGCCTTTCACGA
GGCGGTGCTCAGTGTGACCGCGTGGACATGCGCCAGTTCGGCCAGGACGCAGAACCAGCACCGCGTCGCGGGGGATTGCC
GCACCACTGCGCTGACCGCCGCGATCAGGCTGATCGACCACAACCTTCACAACCCGGTCACCGTCGAATGGCTCGCCAGG
ACGCTGGGCGTCAGCCGCCGGTCGCTCGAACAGGCGTTCGGCGAAGCTTTCGACACCTCGCCCGGCCGCTACATCAAGGT
GCTGCGGCTCAATTCGCTGCGCCAGATGCTGCGCGATCCGTCCAATGCCGAGGTCAGCATCGGCGATCTGGCCGCACGCT
GCGGGCTTTGGCACCAGGGGCGGCTCGCCAGCGAGTTCCGCGAACTGTTCGGCGTCCTGCCCTCCGAAGCGCGCGGTTAG

Upstream 100 bases:

>100_bases
TGAATCTAGCAAAGATGGGTAATTCGGGTGATCCGGATCACGCGCTGCGCTTGCCTGTTACTCGTCTTTTTTTCCTTTTT
TGAAGAGTGACTTGCCGCTC

Downstream 100 bases:

>100_bases
TCGGCCATCCGGCTATCGCGGGGCTGCAGTTTCAGACGACGCTCCCCTCCAGGATTTGCAAGGTCTTGAGGTCGGAGTGG
AAGTCCAGGGCATAGTCGCC

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MYPECAKLPTFLRTVHEDVDAQARSLFTLDQNYMQVDPGPFCGTMATCDFGTGLVVSFEQVNKRVLQVAAVPFGTAQVAL
ILEAGEPTTHNQVGRIRNAGVVMAPGAEARLSAGANTRVCLISIAGEAADHLRSRYNFDPDSLRADIFLDPAETTALRGL
VTEAYQRFSSPQADRSFDQASAAAFHEAVLSVTAWTCASSARTQNQHRVAGDCRTTALTAAIRLIDHNLHNPVTVEWLAR
TLGVSRRSLEQAFGEAFDTSPGRYIKVLRLNSLRQMLRDPSNAEVSIGDLAARCGLWHQGRLASEFRELFGVLPSEARG

Sequences:

>Translated_319_residues
MYPECAKLPTFLRTVHEDVDAQARSLFTLDQNYMQVDPGPFCGTMATCDFGTGLVVSFEQVNKRVLQVAAVPFGTAQVAL
ILEAGEPTTHNQVGRIRNAGVVMAPGAEARLSAGANTRVCLISIAGEAADHLRSRYNFDPDSLRADIFLDPAETTALRGL
VTEAYQRFSSPQADRSFDQASAAAFHEAVLSVTAWTCASSARTQNQHRVAGDCRTTALTAAIRLIDHNLHNPVTVEWLAR
TLGVSRRSLEQAFGEAFDTSPGRYIKVLRLNSLRQMLRDPSNAEVSIGDLAARCGLWHQGRLASEFRELFGVLPSEARG
>Mature_319_residues
MYPECAKLPTFLRTVHEDVDAQARSLFTLDQNYMQVDPGPFCGTMATCDFGTGLVVSFEQVNKRVLQVAAVPFGTAQVAL
ILEAGEPTTHNQVGRIRNAGVVMAPGAEARLSAGANTRVCLISIAGEAADHLRSRYNFDPDSLRADIFLDPAETTALRGL
VTEAYQRFSSPQADRSFDQASAAAFHEAVLSVTAWTCASSARTQNQHRVAGDCRTTALTAAIRLIDHNLHNPVTVEWLAR
TLGVSRRSLEQAFGEAFDTSPGRYIKVLRLNSLRQMLRDPSNAEVSIGDLAARCGLWHQGRLASEFRELFGVLPSEARG

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=98, Percent_Identity=35.7142857142857, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 34735; Mature: 34735

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYPECAKLPTFLRTVHEDVDAQARSLFTLDQNYMQVDPGPFCGTMATCDFGTGLVVSFEQ
CCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCCCCCEECCCCCCCEEEHHH
VNKRVLQVAAVPFGTAQVALILEAGEPTTHNQVGRIRNAGVVMAPGAEARLSAGANTRVC
HHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHCCCCEEEECCCCHHHCCCCCCEEE
LISIAGEAADHLRSRYNFDPDSLRADIFLDPAETTALRGLVTEAYQRFSSPQADRSFDQA
EEEECCHHHHHHHHHCCCCHHHCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
SAAAFHEAVLSVTAWTCASSARTQNQHRVAGDCRTTALTAAIRLIDHNLHNPVTVEWLAR
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
TLGVSRRSLEQAFGEAFDTSPGRYIKVLRLNSLRQMLRDPSNAEVSIGDLAARCGLWHQG
HHCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCCCCEEEHHHHHHHHCCCCCC
RLASEFRELFGVLPSEARG
CHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MYPECAKLPTFLRTVHEDVDAQARSLFTLDQNYMQVDPGPFCGTMATCDFGTGLVVSFEQ
CCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCCCCCEECCCCCCCEEEHHH
VNKRVLQVAAVPFGTAQVALILEAGEPTTHNQVGRIRNAGVVMAPGAEARLSAGANTRVC
HHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHCCCCEEEECCCCHHHCCCCCCEEE
LISIAGEAADHLRSRYNFDPDSLRADIFLDPAETTALRGLVTEAYQRFSSPQADRSFDQA
EEEECCHHHHHHHHHCCCCHHHCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
SAAAFHEAVLSVTAWTCASSARTQNQHRVAGDCRTTALTAAIRLIDHNLHNPVTVEWLAR
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
TLGVSRRSLEQAFGEAFDTSPGRYIKVLRLNSLRQMLRDPSNAEVSIGDLAARCGLWHQG
HHCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCCCCEEEHHHHHHHHCCCCCC
RLASEFRELFGVLPSEARG
CHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]