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The map label for this gene is hpcB [H]

Identifier: 87200620

GI number: 87200620

Start: 2818565

End: 2819437

Strand: Reverse

Name: hpcB [H]

Synonym: Saro_2607

Alternate gene names: 87200620

Gene position: 2819437-2818565 (Counterclockwise)

Preceding gene: 87200624

Following gene: 87200617

Centisome position: 79.16

GC content: 63.69

Gene sequence:

>873_bases
ATGGGCGAGATCATCGGAGCCGGCATCGTCGCGCACGCGCCGACCATCATGATGTCGAAGGAAGACCGCTTCGAGCTTAA
CGAAGGCAAGGAAATCAGCCTTGTACCGGGCCTGCATCGGCTGAAGGCCGAAGTGCTCGACGTGCTGAAACCCGACACCA
TCGTGCTGTTCGACACGCACTGGTTCACCATCGTCGAATTCTGCATCGCCGCGCACGGCAGGCGGACCGGGCTCTACACC
TCGGACGAATTGCCGCGCGGAATGAAGCAGATACCCTACGACCTGAAAGGCAGTCCGGCGCTCGCCCACGCGATGGCGGA
ACGGGCGACCGAGGCCGGGGTACGCACCACGGCCATCGACGACCCCTGGCTTCCGATCCACTATCCCACTGTAAACCTTT
CCACTTTCCTTCAGGGAGAGGAGGAGTGGGTCTCCGTCAGCCACGCCTATTCGGGGGAGCCGGACGACTTCCTGAAGGTG
GGCGAGGGGATCGGCAGGGCGATCGCGGAATCCGACAAGCGGGTCGTTCTCATCGCCAGCGGATCGATGAGCCACCGGTT
CTGGCCATTCAGGCAGATCCCGCTGCACGAAGCCTCTGATCCCGTTCACATTTCCAGCCCGGAGGCGCGGGAGGCCGACA
TGGAGCGGTTGCGCTGGTTTGCCGAAGGCAACCATGCGCAAGTCATTGATACGATGCCCGATTACTTGCGCCACGCGCCA
GAGGCCCGCTTCGGGCACTACCTGATGATGGCGGCGGCATGCGGCGGACGCGGCTGGAAGGCGAAGGGGCGGCAGTTCTC
GGACTACGAGAACGCGACCGGGACCAGCCAGGTCCACGTCTGGTTCGACAAGCCGGACGGCGGCTGGAACTAG

Upstream 100 bases:

>100_bases
GGACTTGGCATAAGCGGACAGACTCGACGGGGGCTATTCAGGTCGGCCGGACTTGCGCCTAACAGGATCGGCGATCCTCA
GGACAAGGAACGCGATCACC

Downstream 100 bases:

>100_bases
GGTACAGGAAAGGGGGCGCGAGGCCCCCTTTCCGTTGTCCCGTGTGCATCCAGGTGCCTTTAGGTGCTTTTAGGTGCCGT
TCAGGACTCGGCTCTCGATC

Product: extradiol ring-cleavage dioxygenase III subunit B

Products: NA

Alternate protein names: Homoprotocatechuate 2,3-dioxygenase; HPC dioxygenase [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MGEIIGAGIVAHAPTIMMSKEDRFELNEGKEISLVPGLHRLKAEVLDVLKPDTIVLFDTHWFTIVEFCIAAHGRRTGLYT
SDELPRGMKQIPYDLKGSPALAHAMAERATEAGVRTTAIDDPWLPIHYPTVNLSTFLQGEEEWVSVSHAYSGEPDDFLKV
GEGIGRAIAESDKRVVLIASGSMSHRFWPFRQIPLHEASDPVHISSPEAREADMERLRWFAEGNHAQVIDTMPDYLRHAP
EARFGHYLMMAAACGGRGWKAKGRQFSDYENATGTSQVHVWFDKPDGGWN

Sequences:

>Translated_290_residues
MGEIIGAGIVAHAPTIMMSKEDRFELNEGKEISLVPGLHRLKAEVLDVLKPDTIVLFDTHWFTIVEFCIAAHGRRTGLYT
SDELPRGMKQIPYDLKGSPALAHAMAERATEAGVRTTAIDDPWLPIHYPTVNLSTFLQGEEEWVSVSHAYSGEPDDFLKV
GEGIGRAIAESDKRVVLIASGSMSHRFWPFRQIPLHEASDPVHISSPEAREADMERLRWFAEGNHAQVIDTMPDYLRHAP
EARFGHYLMMAAACGGRGWKAKGRQFSDYENATGTSQVHVWFDKPDGGWN
>Mature_289_residues
GEIIGAGIVAHAPTIMMSKEDRFELNEGKEISLVPGLHRLKAEVLDVLKPDTIVLFDTHWFTIVEFCIAAHGRRTGLYTS
DELPRGMKQIPYDLKGSPALAHAMAERATEAGVRTTAIDDPWLPIHYPTVNLSTFLQGEEEWVSVSHAYSGEPDDFLKVG
EGIGRAIAESDKRVVLIASGSMSHRFWPFRQIPLHEASDPVHISSPEAREADMERLRWFAEGNHAQVIDTMPDYLRHAPE
ARFGHYLMMAAACGGRGWKAKGRQFSDYENATGTSQVHVWFDKPDGGWN

Specific function: Transforms homoprotocatechuic acid (HPC) into 5- carboxymethyl-2-hydroxy-muconic semialdehyde (CHMS) [H]

COG id: COG3384

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011984
- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.15 [H]

Molecular weight: Translated: 32363; Mature: 32232

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEIIGAGIVAHAPTIMMSKEDRFELNEGKEISLVPGLHRLKAEVLDVLKPDTIVLFDTH
CCCCCCCCHHHHCCEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEECCH
WFTIVEFCIAAHGRRTGLYTSDELPRGMKQIPYDLKGSPALAHAMAERATEAGVRTTAID
HHHHHHHHHHHCCCCCCCCCCHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEECC
DPWLPIHYPTVNLSTFLQGEEEWVSVSHAYSGEPDDFLKVGEGIGRAIAESDKRVVLIAS
CCCCEEEECCCCHHHHHCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEC
GSMSHRFWPFRQIPLHEASDPVHISSPEAREADMERLRWFAEGNHAQVIDTMPDYLRHAP
CCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCC
EARFGHYLMMAAACGGRGWKAKGRQFSDYENATGTSQVHVWFDKPDGGWN
CCHHHHHEEEHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure 
GEIIGAGIVAHAPTIMMSKEDRFELNEGKEISLVPGLHRLKAEVLDVLKPDTIVLFDTH
CCCCCCCHHHHCCEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEECCH
WFTIVEFCIAAHGRRTGLYTSDELPRGMKQIPYDLKGSPALAHAMAERATEAGVRTTAID
HHHHHHHHHHHCCCCCCCCCCHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEECC
DPWLPIHYPTVNLSTFLQGEEEWVSVSHAYSGEPDDFLKVGEGIGRAIAESDKRVVLIAS
CCCCEEEECCCCHHHHHCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEC
GSMSHRFWPFRQIPLHEASDPVHISSPEAREADMERLRWFAEGNHAQVIDTMPDYLRHAP
CCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCC
EARFGHYLMMAAACGGRGWKAKGRQFSDYENATGTSQVHVWFDKPDGGWN
CCHHHHHEEEHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2261999 [H]