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The map label for this gene is hsaB [H]

Identifier: 87200595

GI number: 87200595

Start: 2787539

End: 2788027

Strand: Reverse

Name: hsaB [H]

Synonym: Saro_2582

Alternate gene names: 87200595

Gene position: 2788027-2787539 (Counterclockwise)

Preceding gene: 87200596

Following gene: 87200594

Centisome position: 78.28

GC content: 64.42

Gene sequence:

>489_bases
ATGATCGATCCCCGCCATTTCCGCAACGTGCTGGGCGCCTGGCCGACCGGCGTCTGTGTCATCACTGCCGTCGACGATGA
TGGGCAAAGGCATGGGCTGGTGGTGGGCTCGTTCACTTCGATCTCGCTCGATCCCCCGCTGGTCGGCTTCTTTCCGGACA
AACGTTCCAGCACCTGGCCGAAGATCGCCGCGACGGGGCGTTTCTGCGCCAACGTGCTGGGTTCGGAACAGCTTGATCTG
TGCAAGCGCTTTGCCGCGCGCGCGGACGACAAGTTTGCCGAACTGGCGCATGGTCACACGCCTTCCGGACTGCCGCTGCT
GGACGATGCGATCGCCTGGATCGATTGCAGTATCGAACGGGTCGAGGACATCGGCGACCACCTGCTGGTCGTCGGCGCGG
TCGAGGCCCTCGATCGACGCGAGGAAGGCACGCCGCTGCTGTTCTTCCGTGGCCAGTATCACGACGTCACGCACCTGCCC
GGCGTCTGA

Upstream 100 bases:

>100_bases
AACCCGCTCGAGCATCGAGGTGCGCTGCATCGCCTTCTTCGAATAGGAATTCGGCGGGAAGATTGTTGACGCGCGCCGCG
TTTGACGACAGGCAGGCGCG

Downstream 100 bases:

>100_bases
GAACCAGCGAAAATCCTAAAAATTGTTGGCGTTTTGACATCCGTCAACGCGCGGCCGGACGGTCGCGCCTAATCCTGACC
TCGACGAAGCGGGAACAAGA

Product: flavin reductase-like protein

Products: NAD(+); reduced flavin [C]

Alternate protein names: Flavin:NADH reductase [H]

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MIDPRHFRNVLGAWPTGVCVITAVDDDGQRHGLVVGSFTSISLDPPLVGFFPDKRSSTWPKIAATGRFCANVLGSEQLDL
CKRFAARADDKFAELAHGHTPSGLPLLDDAIAWIDCSIERVEDIGDHLLVVGAVEALDRREEGTPLLFFRGQYHDVTHLP
GV

Sequences:

>Translated_162_residues
MIDPRHFRNVLGAWPTGVCVITAVDDDGQRHGLVVGSFTSISLDPPLVGFFPDKRSSTWPKIAATGRFCANVLGSEQLDL
CKRFAARADDKFAELAHGHTPSGLPLLDDAIAWIDCSIERVEDIGDHLLVVGAVEALDRREEGTPLLFFRGQYHDVTHLP
GV
>Mature_162_residues
MIDPRHFRNVLGAWPTGVCVITAVDDDGQRHGLVVGSFTSISLDPPLVGFFPDKRSSTWPKIAATGRFCANVLGSEQLDL
CKRFAARADDKFAELAHGHTPSGLPLLDDAIAWIDCSIERVEDIGDHLLVVGAVEALDRREEGTPLLFFRGQYHDVTHLP
GV

Specific function: Catalyzes the reduction of free flavins (FMN or FAD) by NADH. Subsequently, the reduced flavins diffuse to the HsaA oxygenase subunit [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=156, Percent_Identity=26.2820512820513, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 17701; Mature: 17701

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDPRHFRNVLGAWPTGVCVITAVDDDGQRHGLVVGSFTSISLDPPLVGFFPDKRSSTWP
CCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEECCEEEECCCCEEEECCCCCCCCCC
KIAATGRFCANVLGSEQLDLCKRFAARADDKFAELAHGHTPSGLPLLDDAIAWIDCSIER
CHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHH
VEDIGDHLLVVGAVEALDRREEGTPLLFFRGQYHDVTHLPGV
HHHCCHHEEEHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MIDPRHFRNVLGAWPTGVCVITAVDDDGQRHGLVVGSFTSISLDPPLVGFFPDKRSSTWP
CCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEECCEEEECCCCEEEECCCCCCCCCC
KIAATGRFCANVLGSEQLDLCKRFAARADDKFAELAHGHTPSGLPLLDDAIAWIDCSIER
CHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHH
VEDIGDHLLVVGAVEALDRREEGTPLLFFRGQYHDVTHLPGV
HHHCCHHEEEHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA