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The map label for this gene is fixI [H]

Identifier: 87200585

GI number: 87200585

Start: 2775281

End: 2777404

Strand: Reverse

Name: fixI [H]

Synonym: Saro_2572

Alternate gene names: 87200585

Gene position: 2777404-2775281 (Counterclockwise)

Preceding gene: 87200586

Following gene: 87200584

Centisome position: 77.98

GC content: 68.88

Gene sequence:

>2124_bases
GTGAACACTCTCGCGCGCAGGTCGGCCGAGCCAGTGCCCGCGGTCCACACCGTTCTGGCTGTCCCGGGCATGCATTGCGC
GGGTTGCATGGGCAAGGTCGAGCGCGCGCTCGGCGCGGTCGAGGGCGTTGCCAGCGCGCGCACAAACCTGACCGCGCGCA
CGGTCGAGGTCATCCATGCACCCGCGATCGAAACGCCCGCGCTCGTTGCCGCACTTGCCGCAACCGGCTTCGAGGCCCAG
CCGCGCGAGATCGAGGCCGAGCCGGTATCTGCGGTGAAGCCGCTGCTCGCGCCGCTTGGCGTGGCCGCCTTCGCCTGCAT
GAACGTCATGCTCATGTCGGTCAGCGTCTGGTCGGGGGCGGATGGCACGACGCGGTCGCTGTTCCACTGGGTCTCGGCGC
TGATCGGCGTTCCCGCCATCGTCTATGCCGGGCGGCCATTCTTCGCCTCGGCGTGGAAGGTGCTGCGCCATGGCAGGACC
AACATGGACGTGCCGATCTCCATCGGCGTCACCATCGCCACCGGCCTCTCGTTCTATGAAACGCTGACCGGCGGGGCAGA
GGCGTGGTTCGACGGCGTTCTCATGCTCCTGATGTTCCTCCTGGCAGGCCGCGTGCTCGATGCGATGATGCGCGACCGCG
CCCGCTCGGGCGTCGATGCGCTGCTGCGCCAGGTTGCCACCGGCACGATGGTGGTCGATGCCGACGGTTCGACCCGCTGG
GTGGAGGCAAAGTCGCTCGAGCCTGGCATGATCATGCGGGTTGCCTCGGGCGAGCGCCTTGCGGCCGATGGTGCGATCGT
TCGCGGCACCAGCCGGTTCGACCAGTCGTTGCTTACCGGTGAGAGCGAACCCGTCAACGCCGGGCCGGGAAGCATTGTCC
ACGCGGGCACGCTCAACGTCGAACTGCCGGTCGATGTGCGCGTGACCGCCATCGGCCGCGATACCGCCTTGTCAGAGATC
GCCCGCCTGATGGAAGCGGCCGGCCAGTCGCGGTCGAAGTACGTGCGCATTGCCGACCGTGCCTCGCGGCTTTACGCACC
AGCCGTCCATACGCTCGCGGCGCTGACGTTCGTCGGCTGGATGATCGCGGGCGCAGGCGTCTACAAGTCGCTGGTCATCG
CGATCGCAGTGCTCATCATTACCTGCCCTTGTGCGCTTGGCCTTGCGGTGCCGGTGGCGCAGGTCGTTGCCGCGGGTGCG
CTGATGAAGCGCGGCGTGTTGGTGAAGGACGGCTCCGCGCTCGAACGCATGGCCGAAGTCGACCGCCTCCTGCTCGACAA
GACCGGTACGCTGACGCTCGGTCGCCCGGTTCCCGACGAAGCGGCCCTCGACGCCCTGTCCGGCGCCGAAGCCTCTGTCG
CCCTCGCGCTCGCCAGTCACAGTCGCCACCCCCTGTCGCTGGGTCTCGTGCAGGCCCTCGGCGCGCGCGGTGTCCTCGCG
GCGGCCCTGGAAGATGTGGTGGAAGAGCCGGGGAAGGGCGTGAGCGGCAAATGGAATGGCCTTGCGGTGTCGCTCGGTCG
CCCGCAATCGGCCCAGGGCATGGCCACGGCGCTTCATGTTGAGGGCGGGCCGGTCCGTGTCATCGCCTTTGCCGACCGGC
TTCGTCCCGACGCCGAAGCCGCGCTCGGGCAGTTGTCGAGCATGGGTGTTCCAGCCTCCATCCTCTCCGGCGATTCCATC
CCTGCGGTCGCCGCCGTCTCGCGCGCGACGGGTCTCACCGCGCAGGCCGCCGCCAGCCCGATGGAGAAGCAGGAAGCCAT
CGAGCGTCTCCAGGCAGCGGGGCGCACCGTGCTTATGGCGGGCGACGGGTTGAACGATGGTCCGGCGCTTGCCGCCGCCA
ACGCCTCGATCGCCCCCGGTTCTGCCAGCGACGTCGGTCGGCAGGCTGCGGACATGGTGTTCACCGGCGATTCCCTGCTC
GCCATCCCTCGCGCGGTGCGGGTGGCCCGCGCCACCATGCGGGTCGTGCGCCAGAACTTCGTCCTCGCCATCGGCTACAA
CGCGCTGGCGGTGCCGCTGGCGATCCTTGGCCACGTTACCCCGCTCATCGCTGCCATCGCCATGTCGACCAGCTCGCTTG
TCGTCGTGGCCAATTCGCTGCGTCTTGCCGGAGAAGCGAAATGA

Upstream 100 bases:

>100_bases
TGCCCGGCGTTTACGCTGCCCCGCTTGAAGCCGGTCGCTGGCGCCTGCGGGTGACTGTCGAGGCCGAAGGCAAGGTCTGG
CGCACGGTCGGGGAAGTGTT

Downstream 100 bases:

>100_bases
ACGGCATCGCCTTCATGCTCCCGATCGCCCTTGGCATGGGCCTGTTCGGACTGGCCTCCTTCTTCTGGGCTCTCAAGCGC
GGGCAGTTCAGCGATCTCGA

Product: heavy metal translocating P-type ATPase

Products: ADP; Orthophosphate. [C]

Alternate protein names: E1-E2 type cation ATPase FixI [H]

Number of amino acids: Translated: 707; Mature: 707

Protein sequence:

>707_residues
MNTLARRSAEPVPAVHTVLAVPGMHCAGCMGKVERALGAVEGVASARTNLTARTVEVIHAPAIETPALVAALAATGFEAQ
PREIEAEPVSAVKPLLAPLGVAAFACMNVMLMSVSVWSGADGTTRSLFHWVSALIGVPAIVYAGRPFFASAWKVLRHGRT
NMDVPISIGVTIATGLSFYETLTGGAEAWFDGVLMLLMFLLAGRVLDAMMRDRARSGVDALLRQVATGTMVVDADGSTRW
VEAKSLEPGMIMRVASGERLAADGAIVRGTSRFDQSLLTGESEPVNAGPGSIVHAGTLNVELPVDVRVTAIGRDTALSEI
ARLMEAAGQSRSKYVRIADRASRLYAPAVHTLAALTFVGWMIAGAGVYKSLVIAIAVLIITCPCALGLAVPVAQVVAAGA
LMKRGVLVKDGSALERMAEVDRLLLDKTGTLTLGRPVPDEAALDALSGAEASVALALASHSRHPLSLGLVQALGARGVLA
AALEDVVEEPGKGVSGKWNGLAVSLGRPQSAQGMATALHVEGGPVRVIAFADRLRPDAEAALGQLSSMGVPASILSGDSI
PAVAAVSRATGLTAQAAASPMEKQEAIERLQAAGRTVLMAGDGLNDGPALAAANASIAPGSASDVGRQAADMVFTGDSLL
AIPRAVRVARATMRVVRQNFVLAIGYNALAVPLAILGHVTPLIAAIAMSTSSLVVVANSLRLAGEAK

Sequences:

>Translated_707_residues
MNTLARRSAEPVPAVHTVLAVPGMHCAGCMGKVERALGAVEGVASARTNLTARTVEVIHAPAIETPALVAALAATGFEAQ
PREIEAEPVSAVKPLLAPLGVAAFACMNVMLMSVSVWSGADGTTRSLFHWVSALIGVPAIVYAGRPFFASAWKVLRHGRT
NMDVPISIGVTIATGLSFYETLTGGAEAWFDGVLMLLMFLLAGRVLDAMMRDRARSGVDALLRQVATGTMVVDADGSTRW
VEAKSLEPGMIMRVASGERLAADGAIVRGTSRFDQSLLTGESEPVNAGPGSIVHAGTLNVELPVDVRVTAIGRDTALSEI
ARLMEAAGQSRSKYVRIADRASRLYAPAVHTLAALTFVGWMIAGAGVYKSLVIAIAVLIITCPCALGLAVPVAQVVAAGA
LMKRGVLVKDGSALERMAEVDRLLLDKTGTLTLGRPVPDEAALDALSGAEASVALALASHSRHPLSLGLVQALGARGVLA
AALEDVVEEPGKGVSGKWNGLAVSLGRPQSAQGMATALHVEGGPVRVIAFADRLRPDAEAALGQLSSMGVPASILSGDSI
PAVAAVSRATGLTAQAAASPMEKQEAIERLQAAGRTVLMAGDGLNDGPALAAANASIAPGSASDVGRQAADMVFTGDSLL
AIPRAVRVARATMRVVRQNFVLAIGYNALAVPLAILGHVTPLIAAIAMSTSSLVVVANSLRLAGEAK
>Mature_707_residues
MNTLARRSAEPVPAVHTVLAVPGMHCAGCMGKVERALGAVEGVASARTNLTARTVEVIHAPAIETPALVAALAATGFEAQ
PREIEAEPVSAVKPLLAPLGVAAFACMNVMLMSVSVWSGADGTTRSLFHWVSALIGVPAIVYAGRPFFASAWKVLRHGRT
NMDVPISIGVTIATGLSFYETLTGGAEAWFDGVLMLLMFLLAGRVLDAMMRDRARSGVDALLRQVATGTMVVDADGSTRW
VEAKSLEPGMIMRVASGERLAADGAIVRGTSRFDQSLLTGESEPVNAGPGSIVHAGTLNVELPVDVRVTAIGRDTALSEI
ARLMEAAGQSRSKYVRIADRASRLYAPAVHTLAALTFVGWMIAGAGVYKSLVIAIAVLIITCPCALGLAVPVAQVVAAGA
LMKRGVLVKDGSALERMAEVDRLLLDKTGTLTLGRPVPDEAALDALSGAEASVALALASHSRHPLSLGLVQALGARGVLA
AALEDVVEEPGKGVSGKWNGLAVSLGRPQSAQGMATALHVEGGPVRVIAFADRLRPDAEAALGQLSSMGVPASILSGDSI
PAVAAVSRATGLTAQAAASPMEKQEAIERLQAAGRTVLMAGDGLNDGPALAAANASIAPGSASDVGRQAADMVFTGDSLL
AIPRAVRVARATMRVVRQNFVLAIGYNALAVPLAILGHVTPLIAAIAMSTSSLVVVANSLRLAGEAK

Specific function: FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=776, Percent_Identity=27.5773195876289, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI115529486, Length=557, Percent_Identity=28.1867145421903, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI55743073, Length=493, Percent_Identity=29.2089249492901, Blast_Score=183, Evalue=5e-46,
Organism=Escherichia coli, GI1786691, Length=735, Percent_Identity=32.9251700680272, Blast_Score=295, Evalue=9e-81,
Organism=Escherichia coli, GI1789879, Length=716, Percent_Identity=30.4469273743017, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1786914, Length=434, Percent_Identity=27.8801843317972, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI2367363, Length=381, Percent_Identity=25.1968503937008, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17556548, Length=339, Percent_Identity=32.7433628318584, Blast_Score=163, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6319772, Length=614, Percent_Identity=29.3159609120521, Blast_Score=224, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6320475, Length=623, Percent_Identity=26.3242375601926, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6321430, Length=257, Percent_Identity=28.7937743190661, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6325221, Length=257, Percent_Identity=28.4046692607004, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI221329854, Length=512, Percent_Identity=28.515625, Blast_Score=174, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR006403
- InterPro:   IPR000695
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00702 Hydrolase [H]

EC number: 3.6.3.4 [C]

Molecular weight: Translated: 72583; Mature: 72583

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS00154 ATPASE_E1_E2 ; PS50846 HMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLARRSAEPVPAVHTVLAVPGMHCAGCMGKVERALGAVEGVASARTNLTARTVEVIHA
CCCHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
PAIETPALVAALAATGFEAQPREIEAEPVSAVKPLLAPLGVAAFACMNVMLMSVSVWSGA
CCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DGTTRSLFHWVSALIGVPAIVYAGRPFFASAWKVLRHGRTNMDVPISIGVTIATGLSFYE
CCHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHH
TLTGGAEAWFDGVLMLLMFLLAGRVLDAMMRDRARSGVDALLRQVATGTMVVDADGSTRW
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCEEE
VEAKSLEPGMIMRVASGERLAADGAIVRGTSRFDQSLLTGESEPVNAGPGSIVHAGTLNV
EECCCCCCCEEEEECCCCEEECCCCEEECCHHHHHHHHCCCCCCCCCCCCCEEEECEEEE
ELPVDVRVTAIGRDTALSEIARLMEAAGQSRSKYVRIADRASRLYAPAVHTLAALTFVGW
EECCEEEEEEECCHHHHHHHHHHHHHHCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHH
MIAGAGVYKSLVIAIAVLIITCPCALGLAVPVAQVVAAGALMKRGVLVKDGSALERMAEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHH
DRLLLDKTGTLTLGRPVPDEAALDALSGAEASVALALASHSRHPLSLGLVQALGARGVLA
HHHHHCCCCCEEECCCCCCHHHHHHHCCCCHHEEEEEHHCCCCCHHHHHHHHHCCCHHHH
AALEDVVEEPGKGVSGKWNGLAVSLGRPQSAQGMATALHVEGGPVRVIAFADRLRPDAEA
HHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCEEEEEEHHHCCCCHHH
ALGQLSSMGVPASILSGDSIPAVAAVSRATGLTAQAAASPMEKQEAIERLQAAGRTVLMA
HHHHHHHCCCCHHHHCCCCCCHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCEEEEE
GDGLNDGPALAAANASIAPGSASDVGRQAADMVFTGDSLLAIPRAVRVARATMRVVRQNF
CCCCCCCCCEEECCCCCCCCCHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHHHHCE
VLAIGYNALAVPLAILGHVTPLIAAIAMSTSSLVVVANSLRLAGEAK
EEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MNTLARRSAEPVPAVHTVLAVPGMHCAGCMGKVERALGAVEGVASARTNLTARTVEVIHA
CCCHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
PAIETPALVAALAATGFEAQPREIEAEPVSAVKPLLAPLGVAAFACMNVMLMSVSVWSGA
CCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DGTTRSLFHWVSALIGVPAIVYAGRPFFASAWKVLRHGRTNMDVPISIGVTIATGLSFYE
CCHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHH
TLTGGAEAWFDGVLMLLMFLLAGRVLDAMMRDRARSGVDALLRQVATGTMVVDADGSTRW
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCEEE
VEAKSLEPGMIMRVASGERLAADGAIVRGTSRFDQSLLTGESEPVNAGPGSIVHAGTLNV
EECCCCCCCEEEEECCCCEEECCCCEEECCHHHHHHHHCCCCCCCCCCCCCEEEECEEEE
ELPVDVRVTAIGRDTALSEIARLMEAAGQSRSKYVRIADRASRLYAPAVHTLAALTFVGW
EECCEEEEEEECCHHHHHHHHHHHHHHCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHH
MIAGAGVYKSLVIAIAVLIITCPCALGLAVPVAQVVAAGALMKRGVLVKDGSALERMAEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHH
DRLLLDKTGTLTLGRPVPDEAALDALSGAEASVALALASHSRHPLSLGLVQALGARGVLA
HHHHHCCCCCEEECCCCCCHHHHHHHCCCCHHEEEEEHHCCCCCHHHHHHHHHCCCHHHH
AALEDVVEEPGKGVSGKWNGLAVSLGRPQSAQGMATALHVEGGPVRVIAFADRLRPDAEA
HHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCEEEEEEHHHCCCCHHH
ALGQLSSMGVPASILSGDSIPAVAAVSRATGLTAQAAASPMEKQEAIERLQAAGRTVLMA
HHHHHHHCCCCHHHHCCCCCCHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCEEEEE
GDGLNDGPALAAANASIAPGSASDVGRQAADMVFTGDSLLAIPRAVRVARATMRVVRQNF
CCCCCCCCCEEECCCCCCCCCHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHHHHCE
VLAIGYNALAVPLAILGHVTPLIAAIAMSTSSLVVVANSLRLAGEAK
EEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Cu [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O [C]

Specific reaction: ATP + H2O = ADP + Orthophosphate. [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2536685; 11481432 [H]