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The map label for this gene is sir2 [H]

Identifier: 87200571

GI number: 87200571

Start: 2760303

End: 2761937

Strand: Direct

Name: sir2 [H]

Synonym: Saro_2558

Alternate gene names: 87200571

Gene position: 2760303-2761937 (Clockwise)

Preceding gene: 87200570

Following gene: 87200572

Centisome position: 77.5

GC content: 64.4

Gene sequence:

>1635_bases
ATGTATCAGTACGACAGCTATGACCAGGCAATGGTCGATGCCCGCGTCGCCGAATTCCGCGACCAGGTGCAGCGGCGCCT
TGCCGGCGACATCACCGAGGACCAGTTCAAGCCGCTGCGCCTGAAGAACGGCCTCTATCTCCAGCTTCATGCCTACATGC
TGCGCGTGGCTGTGCCCTATGGCACGCTGAACAGCCGGCAGATGCGCATGCTGGGCGACATCGCGGACAAGTACGACCGC
GGATACGGCCACTTCACCACGCGCCAGAACATCCAGTACAACTGGATCAAGCTGGAAGACGCGCCCGACATCCTGGCGGA
CCTCGCCTCGGTCGAGATGCACGCGATCCAGACCAGCGGCAACTGCATCCGCAACACCACCGCCGACCAGTTCGCCGGTG
CCGCGGCCGACGAGCTGGTCGATCCGCGCCCCTATGCCGAACTGCTGCGCCAGTGGTCGACGTTCCATCCGGAGTTCGAC
TACCTGCCGCGCAAGTTCAAGATCGCGATCATCGGCAGCGAAACCGACCGCGCGGCGATGAAGCTGCACGACATCGGCAT
CCAGCTCGTGAAGAACGACGCGGGCGAGATCGGCTGCCGGTTCTACGTCGGTGGCGGCATGGGCCGCACGCCGATGATCG
CCCCGGTGATCGGCGACTTCGTGCCGCCGCTGCAGATGATCTCCTATGCCGAGGCCTGCCTGCGCGTGTACAACCGCTAT
GGCCGCCGCGACAACAAGTACAAGGCGCGCATCAAGATCCTCGTCCACGAGATCGGCGCCGATGAATATCGCCGCCAGGT
CGAGGAAGAGTTCAAGCACATCCTGACGCTGGGCCTCGAGCCGCCTGCCGCCGAACTGGAGCGCATCAAGGGCTTCTTCG
AGCAGCCGGGCTTCGATGCCAACGCATCGGACGAGCTGGACCTGTCCGATCCCGACTTCGCACTGTGGGTGGACCAGAAC
GTCCATGCCCACAAGGCCAAGGGCTATGCCATCGTCAACATCAGCCTGAAGCCGGTCGGCGGCATTCCGGGCGATGCGAC
GGCCGACCAGATCCGCCTTATGGCGGACCTCGCGCTCGAGCATTCGTTCGACGAGCTGCGCGTTACCCATGCGCAGAACA
TCGTCCTGCCGCACGTGAAGAAGGCGGACCTCCATGCGATCTGGCAGAAGCTGGACGCGGCGGGCCTGGCGAATGCGAAC
CTCGACCTGATCGGCGACATCATCGCTTGCCCGGGCCTCGACTATTGCAGCCTTGCCAATGCCCGTTCGATCCCGGTGGC
GCAGAAGATTTCGGAGCGCTTCGCAGACCTCGGCCGCCAGCGCGAGCTGGGCGAGCTGAAGCTGAAGATCTCGGGCTGCA
TCAACGCCTGCGGACATCACCACGCCGGCCACATCGGCATCCTCGGCGTCGACCGCAAGGGCGTGGAGAACTACCAGCTC
CTGCTCGGCGGGTGCGAGGGCGCGGATACCTCGCTCGGCACGATCACCGGCCCCGGCTTCAACGAGGACGGGATCGTGGA
TGCGGTCGAAAAGGCCACTGACGTCTATCTCGCCCAGCGCGCCGAAGGGGAACGCTTCGTCGACACCTATCGCCGCATCG
GCATGGCCCCGTTCAAGGAGGCAATCTATGGTTGA

Upstream 100 bases:

>100_bases
GCGCACATCAAGGACCGCATTCGCGCCCTCGGGCCCACGGTCCGCCTCGACCTTTCCCTCAAGCCTGCCGACCCTGCCGC
CGGCAACTGGGTGATCTGAG

Downstream 100 bases:

>100_bases
GCAGAACCTGCGTTTCCGGGGCGATGAACCGGTGGCCGATCCGGCCGTGACCGTCGACGCCTTTGCCGAGCAGACCAACG
CGACCGCCGTACGGATCGAG

Product: nitrite and sulfite reductase 4Fe-4S region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MYQYDSYDQAMVDARVAEFRDQVQRRLAGDITEDQFKPLRLKNGLYLQLHAYMLRVAVPYGTLNSRQMRMLGDIADKYDR
GYGHFTTRQNIQYNWIKLEDAPDILADLASVEMHAIQTSGNCIRNTTADQFAGAAADELVDPRPYAELLRQWSTFHPEFD
YLPRKFKIAIIGSETDRAAMKLHDIGIQLVKNDAGEIGCRFYVGGGMGRTPMIAPVIGDFVPPLQMISYAEACLRVYNRY
GRRDNKYKARIKILVHEIGADEYRRQVEEEFKHILTLGLEPPAAELERIKGFFEQPGFDANASDELDLSDPDFALWVDQN
VHAHKAKGYAIVNISLKPVGGIPGDATADQIRLMADLALEHSFDELRVTHAQNIVLPHVKKADLHAIWQKLDAAGLANAN
LDLIGDIIACPGLDYCSLANARSIPVAQKISERFADLGRQRELGELKLKISGCINACGHHHAGHIGILGVDRKGVENYQL
LLGGCEGADTSLGTITGPGFNEDGIVDAVEKATDVYLAQRAEGERFVDTYRRIGMAPFKEAIYG

Sequences:

>Translated_544_residues
MYQYDSYDQAMVDARVAEFRDQVQRRLAGDITEDQFKPLRLKNGLYLQLHAYMLRVAVPYGTLNSRQMRMLGDIADKYDR
GYGHFTTRQNIQYNWIKLEDAPDILADLASVEMHAIQTSGNCIRNTTADQFAGAAADELVDPRPYAELLRQWSTFHPEFD
YLPRKFKIAIIGSETDRAAMKLHDIGIQLVKNDAGEIGCRFYVGGGMGRTPMIAPVIGDFVPPLQMISYAEACLRVYNRY
GRRDNKYKARIKILVHEIGADEYRRQVEEEFKHILTLGLEPPAAELERIKGFFEQPGFDANASDELDLSDPDFALWVDQN
VHAHKAKGYAIVNISLKPVGGIPGDATADQIRLMADLALEHSFDELRVTHAQNIVLPHVKKADLHAIWQKLDAAGLANAN
LDLIGDIIACPGLDYCSLANARSIPVAQKISERFADLGRQRELGELKLKISGCINACGHHHAGHIGILGVDRKGVENYQL
LLGGCEGADTSLGTITGPGFNEDGIVDAVEKATDVYLAQRAEGERFVDTYRRIGMAPFKEAIYG
>Mature_544_residues
MYQYDSYDQAMVDARVAEFRDQVQRRLAGDITEDQFKPLRLKNGLYLQLHAYMLRVAVPYGTLNSRQMRMLGDIADKYDR
GYGHFTTRQNIQYNWIKLEDAPDILADLASVEMHAIQTSGNCIRNTTADQFAGAAADELVDPRPYAELLRQWSTFHPEFD
YLPRKFKIAIIGSETDRAAMKLHDIGIQLVKNDAGEIGCRFYVGGGMGRTPMIAPVIGDFVPPLQMISYAEACLRVYNRY
GRRDNKYKARIKILVHEIGADEYRRQVEEEFKHILTLGLEPPAAELERIKGFFEQPGFDANASDELDLSDPDFALWVDQN
VHAHKAKGYAIVNISLKPVGGIPGDATADQIRLMADLALEHSFDELRVTHAQNIVLPHVKKADLHAIWQKLDAAGLANAN
LDLIGDIIACPGLDYCSLANARSIPVAQKISERFADLGRQRELGELKLKISGCINACGHHHAGHIGILGVDRKGVENYQL
LLGGCEGADTSLGTITGPGFNEDGIVDAVEKATDVYLAQRAEGERFVDTYRRIGMAPFKEAIYG

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

None

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 60608; Mature: 60608

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQYDSYDQAMVDARVAEFRDQVQRRLAGDITEDQFKPLRLKNGLYLQLHAYMLRVAVPY
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEEHHHHHHHHCCCC
GTLNSRQMRMLGDIADKYDRGYGHFTTRQNIQYNWIKLEDAPDILADLASVEMHAIQTSG
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHEEEECCC
NCIRNTTADQFAGAAADELVDPRPYAELLRQWSTFHPEFDYLPRKFKIAIIGSETDRAAM
CEEECCCHHHHCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCHHHH
KLHDIGIQLVKNDAGEIGCRFYVGGGMGRTPMIAPVIGDFVPPLQMISYAEACLRVYNRY
HHHHHCCEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHH
GRRDNKYKARIKILVHEIGADEYRRQVEEEFKHILTLGLEPPAAELERIKGFFEQPGFDA
CCCCCCHHHHEEEEHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
NASDELDLSDPDFALWVDQNVHAHKAKGYAIVNISLKPVGGIPGDATADQIRLMADLALE
CCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
HSFDELRVTHAQNIVLPHVKKADLHAIWQKLDAAGLANANLDLIGDIIACPGLDYCSLAN
CCHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHCCC
ARSIPVAQKISERFADLGRQRELGELKLKISGCINACGHHHAGHIGILGVDRKGVENYQL
CCCCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHCCCCCCCEEEEEECCCCCCCCEEE
LLGGCEGADTSLGTITGPGFNEDGIVDAVEKATDVYLAQRAEGERFVDTYRRIGMAPFKE
EEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHH
AIYG
HHCC
>Mature Secondary Structure
MYQYDSYDQAMVDARVAEFRDQVQRRLAGDITEDQFKPLRLKNGLYLQLHAYMLRVAVPY
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEEHHHHHHHHCCCC
GTLNSRQMRMLGDIADKYDRGYGHFTTRQNIQYNWIKLEDAPDILADLASVEMHAIQTSG
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHEEEECCC
NCIRNTTADQFAGAAADELVDPRPYAELLRQWSTFHPEFDYLPRKFKIAIIGSETDRAAM
CEEECCCHHHHCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCHHHH
KLHDIGIQLVKNDAGEIGCRFYVGGGMGRTPMIAPVIGDFVPPLQMISYAEACLRVYNRY
HHHHHCCEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHH
GRRDNKYKARIKILVHEIGADEYRRQVEEEFKHILTLGLEPPAAELERIKGFFEQPGFDA
CCCCCCHHHHEEEEHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
NASDELDLSDPDFALWVDQNVHAHKAKGYAIVNISLKPVGGIPGDATADQIRLMADLALE
CCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
HSFDELRVTHAQNIVLPHVKKADLHAIWQKLDAAGLANANLDLIGDIIACPGLDYCSLAN
CCHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHCCC
ARSIPVAQKISERFADLGRQRELGELKLKISGCINACGHHHAGHIGILGVDRKGVENYQL
CCCCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHCCCCCCCEEEEEECCCCCCCCEEE
LLGGCEGADTSLGTITGPGFNEDGIVDAVEKATDVYLAQRAEGERFVDTYRRIGMAPFKE
EEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHH
AIYG
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA