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The map label for this gene is ynfL [H]

Identifier: 87200520

GI number: 87200520

Start: 2710035

End: 2710988

Strand: Direct

Name: ynfL [H]

Synonym: Saro_2507

Alternate gene names: 87200520

Gene position: 2710035-2710988 (Clockwise)

Preceding gene: 87200513

Following gene: 87200522

Centisome position: 76.09

GC content: 65.09

Gene sequence:

>954_bases
ATGTCCCCAGATGTCGACATCAAGGCACTTCGCTGCTTTCTCGCGCTGGCGCAGGAGCTTAGCTTCGGTCGCGCGGCGGA
ACGGATGAACCTCACCCAACCCAGCCTGTCTGCCCAGATCCGTAAGCTGGAGGATCAGGTCGGCCATCGCCTGTTCGAGC
GCACCACGCGCGCGGTGCATCTTTCACCGACAGGAAAGGCGCTGATCGAACAGGCCCGCACATTCGTCTCGCAGGCTGAT
GCCTTTGCCGCCCATCTTTCCGCGTGGCGCGAGCGACCGGACCGGCGGATGGTCCTGGGGGCGCCAATCTACACTTTCGA
GCTGCCCGAACACGGCGCCCTCCTCTCCGCGATTGCGCGCGAACTTCCGGACATGTCGCTGCGCGTGGACAACGGCTTCG
CCAACTCGCTGGTCGAAGGACTGATCAAGGGTTCGGTCGACATGGCGATGGTCGTTGCCGCCGCCGTCCCCCACGACCGC
TATCTGGCGGACATGGCCGGGGAAGGCGCGGGCGAGCTGGAGATGCCCGATGGCCTGCAGCGGATCACCTTGAGCGACGA
GCAGATCGGGCTCGCCATCCCCGAAGAACACCCGCTCGCCAGCTACGACATCGTCCCGCCCGAAGCGCTTTCGGGATCGG
TCATCGCCATGCTCGCGCCGCTTCACGGGCGCAGCCTCTACCGCCCGATTTCAGTATGGCTTTCAGCGGCCGGGGCGACC
GGCTTCCTCCCGCCCGAAGCCCACGCCTTCGCGCTGGAACGCTATTGCAGGGAGTATCGCATACCCGCGATCTCGATTGC
ACAGTTCCGCCCGCGAGAGACCGGCAATGTCGTCTATCGTCCTGCCGGAGGGCTCAACGTCCGCACCGAACTTGCCGTAT
TGCGCAGCACGCGAAAGCAGCGTTCCTCGATAGAGGAACGGCTTTGGGACCTGGCCTCAAGCCTCGGCCGCTAG

Upstream 100 bases:

>100_bases
GCGCACTTCGTCGCCTTCGAGGAAAGGCTAGAGAGGGGGTCTTGCTGCGTCCAATAGATTTAATCTTTCGATTTATAGAA
GTCTCCAATATATCTGCCGC

Downstream 100 bases:

>100_bases
CGCGCGGGCACTGCCCGGACGACCGCGCCCCGGCCTTACCTGCGCCATCAACGCGCGGGCGCCGGTCGACATGTGCTGGC
GCAGGAAACGGCTTGCCGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSPDVDIKALRCFLALAQELSFGRAAERMNLTQPSLSAQIRKLEDQVGHRLFERTTRAVHLSPTGKALIEQARTFVSQAD
AFAAHLSAWRERPDRRMVLGAPIYTFELPEHGALLSAIARELPDMSLRVDNGFANSLVEGLIKGSVDMAMVVAAAVPHDR
YLADMAGEGAGELEMPDGLQRITLSDEQIGLAIPEEHPLASYDIVPPEALSGSVIAMLAPLHGRSLYRPISVWLSAAGAT
GFLPPEAHAFALERYCREYRIPAISIAQFRPRETGNVVYRPAGGLNVRTELAVLRSTRKQRSSIEERLWDLASSLGR

Sequences:

>Translated_317_residues
MSPDVDIKALRCFLALAQELSFGRAAERMNLTQPSLSAQIRKLEDQVGHRLFERTTRAVHLSPTGKALIEQARTFVSQAD
AFAAHLSAWRERPDRRMVLGAPIYTFELPEHGALLSAIARELPDMSLRVDNGFANSLVEGLIKGSVDMAMVVAAAVPHDR
YLADMAGEGAGELEMPDGLQRITLSDEQIGLAIPEEHPLASYDIVPPEALSGSVIAMLAPLHGRSLYRPISVWLSAAGAT
GFLPPEAHAFALERYCREYRIPAISIAQFRPRETGNVVYRPAGGLNVRTELAVLRSTRKQRSSIEERLWDLASSLGR
>Mature_316_residues
SPDVDIKALRCFLALAQELSFGRAAERMNLTQPSLSAQIRKLEDQVGHRLFERTTRAVHLSPTGKALIEQARTFVSQADA
FAAHLSAWRERPDRRMVLGAPIYTFELPEHGALLSAIARELPDMSLRVDNGFANSLVEGLIKGSVDMAMVVAAAVPHDRY
LADMAGEGAGELEMPDGLQRITLSDEQIGLAIPEEHPLASYDIVPPEALSGSVIAMLAPLHGRSLYRPISVWLSAAGATG
FLPPEAHAFALERYCREYRIPAISIAQFRPRETGNVVYRPAGGLNVRTELAVLRSTRKQRSSIEERLWDLASSLGR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=230, Percent_Identity=30.4347826086957, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI2367136, Length=217, Percent_Identity=33.1797235023041, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1790399, Length=311, Percent_Identity=26.3665594855305, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788887, Length=282, Percent_Identity=25.886524822695, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI145693105, Length=207, Percent_Identity=25.6038647342995, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786713, Length=92, Percent_Identity=36.9565217391304, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34786; Mature: 34655

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPDVDIKALRCFLALAQELSFGRAAERMNLTQPSLSAQIRKLEDQVGHRLFERTTRAVH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEE
LSPTGKALIEQARTFVSQADAFAAHLSAWRERPDRRMVLGAPIYTFELPEHGALLSAIAR
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCHHHHHHHHH
ELPDMSLRVDNGFANSLVEGLIKGSVDMAMVVAAAVPHDRYLADMAGEGAGELEMPDGLQ
HCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCE
RITLSDEQIGLAIPEEHPLASYDIVPPEALSGSVIAMLAPLHGRSLYRPISVWLSAAGAT
EEEECCCCCCEECCCCCCCCCCCCCCCHHHCCCHHEECCCCCCCHHHHHHHHHHHHCCCC
GFLPPEAHAFALERYCREYRIPAISIAQFRPRETGNVVYRPAGGLNVRTELAVLRSTRKQ
CCCCCHHHHHHHHHHHHHHCCCEEEHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHH
RSSIEERLWDLASSLGR
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SPDVDIKALRCFLALAQELSFGRAAERMNLTQPSLSAQIRKLEDQVGHRLFERTTRAVH
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEE
LSPTGKALIEQARTFVSQADAFAAHLSAWRERPDRRMVLGAPIYTFELPEHGALLSAIAR
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCHHHHHHHHH
ELPDMSLRVDNGFANSLVEGLIKGSVDMAMVVAAAVPHDRYLADMAGEGAGELEMPDGLQ
HCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCE
RITLSDEQIGLAIPEEHPLASYDIVPPEALSGSVIAMLAPLHGRSLYRPISVWLSAAGAT
EEEECCCCCCEECCCCCCCCCCCCCCCHHHCCCHHEECCCCCCCHHHHHHHHHHHHCCCC
GFLPPEAHAFALERYCREYRIPAISIAQFRPRETGNVVYRPAGGLNVRTELAVLRSTRKQ
CCCCCHHHHHHHHHHHHHHCCCEEEHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHH
RSSIEERLWDLASSLGR
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]