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The map label for this gene is truB

Identifier: 87200497

GI number: 87200497

Start: 2686189

End: 2687103

Strand: Reverse

Name: truB

Synonym: Saro_2484

Alternate gene names: 87200497

Gene position: 2687103-2686189 (Counterclockwise)

Preceding gene: 87200498

Following gene: 87200496

Centisome position: 75.45

GC content: 64.7

Gene sequence:

>915_bases
GTGGTCGACGGCTGGATCATCCTCGACAAGCCTCTGGAACTCGGGTCGACTCAAGGGGTTGCGGCGGTAAAGCGCAATCT
GCGGCAGGCCGGCTACGGCAAGTGCAAGGTCGGACACGGCGGGACGCTCGACCCCCTGGCTACCGGGGTGCTTCCGATCG
CGGTGGGCGAGGCGACGAAACTGTGCGGACGGATGCTCGATGCGAGCAAGGTCTATGCTTTCACGGTGGCCTTCGGAACG
GAGACCGACAGCCTCGATCTGGAGGGCGGAATCGTCGCCACCAGCGACGTCCGGCCGCCTCTTGCGGACGTTCATGTCAC
TTTAGGTGCGTTTCGGGATTCCATCGATCAGGTTCCCCCGGCGTACTCGGCCCTGATGGTCGAGGGCAGGCGCGCCTATG
ATCTCGCAAGGGCGGGCGAAACAGTCGAACTGCCCTCGCGCCGGGTCACGATCCATGCACTCACGCTCGATTCGTGGACA
GGAAGCGAGGGCGCGGTGGAAACGGCGACGCTGACCGCGCACGTTTCGAAAGGCACATACATCCGCAGCCTGGCCCGCGA
TATCGCGCGCGCGCTGGGCACGGTGGGCCATGTGACGATGCTGCGACGGCTCAAGGCGGGGCCGTTCGCCATCGAGCAGG
CGATTTCGCTGGACAAATTGAACGCATTGGGTCAAGGCGCGCCCCTTGAACACGTACTCTTGCCGCTGGAGGCAGGGCTG
GTCGACATCCCGGCTCTTGACCTTGATCCCGAGCAGGCGAGGGCGGTCCGTCAGGGCCGCGTTCTGACGGGATTGCCCTT
CGAGGACGGGCTCTACTGGGCGAGGCTTGGCACTGTGCCGGTCGCGCTGGTCGAGCTTTCCGACGGAAACCTGAAGGTTT
CGCGGGGCTTCAACTTACAGGATGTCGCGGAGTAG

Upstream 100 bases:

>100_bases
TTCATCTCGTGGAGCGGCTGATCGGCCCGCCGGTCGACTGGCTGGGACAGCAGCTCGCCAATCTCGCGGTGGCGGTTGCC
GGGCCGGAGCCGATCTGAGC

Downstream 100 bases:

>100_bases
AATACATGTCGATTACCGCCGAGAAGAAGCAGGAAGTCATTCAGAGCAATGCTCGCGCCGAGGGCGACACCGGTTCGCCG
GAAGTCCAGGTCGCGATCCT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MVDGWIILDKPLELGSTQGVAAVKRNLRQAGYGKCKVGHGGTLDPLATGVLPIAVGEATKLCGRMLDASKVYAFTVAFGT
ETDSLDLEGGIVATSDVRPPLADVHVTLGAFRDSIDQVPPAYSALMVEGRRAYDLARAGETVELPSRRVTIHALTLDSWT
GSEGAVETATLTAHVSKGTYIRSLARDIARALGTVGHVTMLRRLKAGPFAIEQAISLDKLNALGQGAPLEHVLLPLEAGL
VDIPALDLDPEQARAVRQGRVLTGLPFEDGLYWARLGTVPVALVELSDGNLKVSRGFNLQDVAE

Sequences:

>Translated_304_residues
MVDGWIILDKPLELGSTQGVAAVKRNLRQAGYGKCKVGHGGTLDPLATGVLPIAVGEATKLCGRMLDASKVYAFTVAFGT
ETDSLDLEGGIVATSDVRPPLADVHVTLGAFRDSIDQVPPAYSALMVEGRRAYDLARAGETVELPSRRVTIHALTLDSWT
GSEGAVETATLTAHVSKGTYIRSLARDIARALGTVGHVTMLRRLKAGPFAIEQAISLDKLNALGQGAPLEHVLLPLEAGL
VDIPALDLDPEQARAVRQGRVLTGLPFEDGLYWARLGTVPVALVELSDGNLKVSRGFNLQDVAE
>Mature_304_residues
MVDGWIILDKPLELGSTQGVAAVKRNLRQAGYGKCKVGHGGTLDPLATGVLPIAVGEATKLCGRMLDASKVYAFTVAFGT
ETDSLDLEGGIVATSDVRPPLADVHVTLGAFRDSIDQVPPAYSALMVEGRRAYDLARAGETVELPSRRVTIHALTLDSWT
GSEGAVETATLTAHVSKGTYIRSLARDIARALGTVGHVTMLRRLKAGPFAIEQAISLDKLNALGQGAPLEHVLLPLEAGL
VDIPALDLDPEQARAVRQGRVLTGLPFEDGLYWARLGTVPVALVELSDGNLKVSRGFNLQDVAE

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=210, Percent_Identity=37.6190476190476, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI2367200, Length=256, Percent_Identity=35.15625, Blast_Score=154, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6324037, Length=144, Percent_Identity=38.8888888888889, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_NOVAD (Q2G5F3)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497754.1
- ProteinModelPortal:   Q2G5F3
- SMR:   Q2G5F3
- STRING:   Q2G5F3
- GeneID:   3916804
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2484
- NMPDR:   fig|48935.1.peg.1943
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   TDDMQGE
- PhylomeDB:   Q2G5F3
- ProtClustDB:   CLSK2524611
- BioCyc:   NARO279238:SARO_2484-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 32190; Mature: 32190

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: ACT_SITE 44-44

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDGWIILDKPLELGSTQGVAAVKRNLRQAGYGKCKVGHGGTLDPLATGVLPIAVGEATK
CCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCHHHHCCEEEEECHHHH
LCGRMLDASKVYAFTVAFGTETDSLDLEGGIVATSDVRPPLADVHVTLGAFRDSIDQVPP
HHHHHHCCCEEEEEEEEECCCCCCEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
AYSALMVEGRRAYDLARAGETVELPSRRVTIHALTLDSWTGSEGAVETATLTAHVSKGTY
CHHHHEECCCHHHHHHHCCCEEECCCCEEEEEEEEEECCCCCCCCEEEEEEEEEECCCHH
IRSLARDIARALGTVGHVTMLRRLKAGPFAIEQAISLDKLNALGQGAPLEHVLLPLEAGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHEEEEHHCCE
VDIPALDLDPEQARAVRQGRVLTGLPFEDGLYWARLGTVPVALVELSDGNLKVSRGFNLQ
EECCCCCCCHHHHHHHHCCCEEECCCCCCCEEEEEECCCEEEEEEECCCCEEEECCCCHH
DVAE
HHCC
>Mature Secondary Structure
MVDGWIILDKPLELGSTQGVAAVKRNLRQAGYGKCKVGHGGTLDPLATGVLPIAVGEATK
CCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCHHHHCCEEEEECHHHH
LCGRMLDASKVYAFTVAFGTETDSLDLEGGIVATSDVRPPLADVHVTLGAFRDSIDQVPP
HHHHHHCCCEEEEEEEEECCCCCCEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
AYSALMVEGRRAYDLARAGETVELPSRRVTIHALTLDSWTGSEGAVETATLTAHVSKGTY
CHHHHEECCCHHHHHHHCCCEEECCCCEEEEEEEEEECCCCCCCCEEEEEEEEEECCCHH
IRSLARDIARALGTVGHVTMLRRLKAGPFAIEQAISLDKLNALGQGAPLEHVLLPLEAGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHEEEEHHCCE
VDIPALDLDPEQARAVRQGRVLTGLPFEDGLYWARLGTVPVALVELSDGNLKVSRGFNLQ
EECCCCCCCHHHHHHHHCCCEEECCCCCCCEEEEEECCCEEEEEEECCCCEEEECCCCHH
DVAE
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA